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The Pharmaceutical Society of Korea (대한약학회)
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Effect of Ginsenoside Rd on Nitric Oxide System Induced by Lipopolysaccharide Plus $TNF-{\alpha}$ in C6 Rat Glioma Cells

  • Choi, Seong-Soo (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Lee, Jin-Koo (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Han, Eun-Jung (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Han, Ki-Jung (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Lee, Han-Kyu (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Lee, Jong-Ho (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University) ;
  • Suh, Hong-Won (Department of Pharmacology, College of Medicine and Institute of Natural Medicine, Hallym University)
  • Published : 2003.05.01
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Abstract

Effects of ginsenosides on nitric oxide (NO) production induced by lipopolysaccharide plus TNF-$\alpha$ (LNT) were examined in C6 rat glioma cells. Among several ginsenosides, ginsenoside Rd showed a complete inhibition against LNT-induced NO production. Ginsenoside Rd attenuated LNT-induced increased phosphorylation of ERK. Among several immediate early gene products, only Jun Band Fra-1 protein levels were increased by LNT, and ginsenoside Rd attenuated Jun Band Fra-1 protein levels induced by LNT. Furthermore, LNT increased AP-1 DNA binding activities, which were partially inhibited by ginsenoside Rd. Our results suggest that ginsenoside Rd exerts an inhibitory action against NO production via blocking phosphorylation of ERK, in turn, suppressing immediate early gene products such as Jun Band Fra-1 in C6 glioma cells.

Keywords

References

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