• 대한본초학회지
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• 제29권6호
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• pp.165-173
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• 2014

Objectives : Platycodon Root is frequently used in traditional medicine to treat inflammatory diseases of the throat. The purpose of this study was to characterize the effect of the EtOH extract of fermented Platycodon grandiflorum on the ameliorative effects on the Antipruritic Effect of atopic dermatitis mouse model induced by compound 48/80 and ovalbumin (OVA)-induced allergic responses in mice. Methods : In the present study, we examined the anti allergic effect of Platycodon grandiflorum (PR) and its fermented production (FPR) in several mouse model. We measured acute ear edema in a mouse model caused by TPA and consecutively histological change of Ear tissue was observed by hematoxylin and eosin (H&E) staining. and also Scratching behaviors by compound 48/80 was investigated. The levels of allergic mediators such as immunoglobulin (Ig) E, and anti-oxidant markers such as SOD and MDA in the sera of OVA induced allergic mice were measured by enzyme-linked immunosorbent assay. Results : FPR inhibited compoud 48/80-induced scratching behavior in mice, as well as acetic acid-induced writhing in mice. The anti-scratching behavioral effect of FPR was more potent than PR. FPR extract significantly decreased the serum levels of IgE and MDA compared with those of OVA control group. Conclusions : These results indicate that Anti allergic effect of Platycodon grandiflorum is enhanced by fermentation with Saccharomyces cerevisae and FPR may be useful for protection from the itching reactions, which are IgE-mediated representative skin allergic diseases.

• Journal of Ginseng Research
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• 제32권1호
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• pp.33-38
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• 2008

To evaluate the antiatopic effect of Korea red ginseng (RG, steamed root of Panax ginseng CA Meyer, Family Araliaceae) fermented by Bifidobacterium longum H-1 (FRG), its inhibitory effect on passive cutaneous anaphylaxis (PCA) reaction and itching in mice was measured. FRG and its ingredient saponin fraction (FSF) potently inhibited PCA reaction and scratching behaviors. FRG at a dose of 200 mg/kg and FSF at a dose of 50 mg/kg significantly inhibited the scratching frequency by 45% and 47%, respectively. FRG and FSF also inhibited the degranulation and protein expression of tumor-necrosis $factor-{\alpha}$ and interleukin-4 of RBL-2H3 cells induced by IgE-complex. However, polysaccharide fraction of FRG (FPF) weakly inhibited it, compared with FSF. The inhibitory effect of FRG against PCA reaction and scratching behaviors more potently inhibited than that of RG. Based on these findings, FRG can improve allergic skin disorders atopic dermatitis by the regulation of $TNF-{\alpha}$, and IL-4 produced by mast cells and basophils and its degranulation.

• 대한의생명과학회지
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• 제19권1호
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• pp.48-54
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• 2013

Atopic dermatitis is a recurrent or chronic eczematous skin disease with severe pruritus and has annually increased in Korea. In this study, we investigated whether Duchesnea chrysantha (Dc) extracts have an anti-inflammatory effect in human monocytic THP-1 cells and human eosinophilic EoL-1 cells. The dried and powdered whole plants of Dc were extracted with 80% EtOH (Dc-1). The residue was diluted with water, and then successively partitioned with n-hexane, EtOAc, and BuOH to produce the n-hexane (Dc-2), EtOAc (Dc-3), BuOH (Dc-4), and the water-soluble fractions (Dc-5), respectively. The mite extract and LPS increased the production of IL-6, IL-8 and MCP-1 in THP-1 cells and the increase was strongly suppressed by Dc-3 extract, as compare with other extracts. Dc-3 also inhibited the release of IL-6 increased by mite extract and LPS in EoL-1 cells. However, Dc-3 extract increased IL-8 production induced by the mite extract and LPS in EoL-1 cells. These results suggest that Dc extract may be used as anti-inflammatory agents in treating allergic disorders such as asthma and atopic dermatitis.

• 대한본초학회지
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• 제24권2호
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• pp.1-5
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• 2009

Objectives : HRHDT has been known as a useful prescription with antibiotic, anti-inflammatory, antioxidative and immunosuppressive activity. To evaluate anti-inflammatory effect of HRHDT, we treated HRHDT-skin in Balb/c mice model induced contact hypersensitivity. Methods : Contact hypersensitivity, a local inflammatory respinse of skin, was induced by spreading the back skin of Balb/c mice with 1% DNCB. HRHDT-skin was prepared by dissolving 3% 1,3-butylene glycol extract of HRHDT in solution and treated 2 weeks on the back skin. Results: HRHDT-skin significantly reduced TEWL and erythema by 0.4-1% of DNCB treatment compared with control group. HRHDT-skin reduced IgE on serum obtained from blood of DNCB-treated Balb/c mice. Histopathological examination showed that thickening of the epidermis, hyperkeratosis and the infiltration of inflammatory cells were found in Balb/c mice under conventional circumstances. Conclusions : These results showed that HRHDT-skin could be used as a pharmaceutical material with antiinflammatory effects by reducing IgE in contact hypersensitivity dermatitis Balb/c mice by DNCB.

• 대한한방소아과학회지
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• 제25권1호
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• pp.1-27
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• 2011

Objectives: The purpose of this study is to investigate the effects of Yijungtang(YJT) on atopic dermatitis in an in-vitro and in-vivo experiment using a RBL-2H3 mast cells and a NC/Nga atopic dermatitis mouse. Methods: In-vitro experiment, IL-4, IL-13 mRNA expression were evaluated by a real-time PCR, IL-4, IL-13 production by ELISA and transcription factor as GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-kB by western blotting. In-vivo experiment, clinical skin score we evaluated by, hematology and Serum total IgE and IgG1 of NC/Nga atopic dermatitis mouse, cytokine level, total number of cell, Immunohistochemical staining and Histological features of auxiliary lymph node(ALN), draining lymph node(DLN), peripheral blood mononuclear cells(PBMCs) and dorsal skin tissue in NC/Nga mouse. Results: YJT decreased IL-4, IL-13 mRNA expression, IL-4, IL-13 production and prominently decreased the expression of mast cell specific transcription factors including GATA-2, NF-AT2, c-Fos and NF-kB. YJT oral administration reduced the levels of skin severity scores. It also decreased the level of inflammatory cytokines such as IL-5, IL-13, histamine and IgE in the serum. It elevated IFN-gamma level in the spleenocyte culture supernatant but decreased. $CD3e^+$, $CD19^+$, $CD4^+$, $CD8^+$, $CD3e^+CD69^+$, $CD11b^+Gr-1^+$, $CCR3^+$ in the PBMCs, $CD4^+$, $CD8^+$, $CD3e^+CD69^+$, $B220^+CD23^+$ in the ALN, $CD4^+$, $CD3e^+CD69^+$ in the ALN and $CD4^+$, $CD11b^+Gr-1^+$ in the dorsal skin. Histological examination showed that infiltration levels of immune cells in the skin of AD-induced NC/Nga mice were much improved by YJT oral administration. Conclusions: The anti-allergic activities of YJT may be mediated by down-regulation of Th2 cytokines, such as IL-4 and IL-13, through the regulation GATA-2, NF-AT2 and NF-kB transcription factors in mast cells. YJT would be regulate molecular mediators and immune cells which are functionally associated with atopic dermatitis induced in NC/Nga mice, and may play an important role in recovering AD symptoms.

• 생명과학회지
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• 제22권7호
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• pp.958-963
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• 2012

본 연구는 항산화 및 알러지성 피부질환에 대한 효과가 있는 안전성이 확보된 새로운 천연 향장 소재 개발을 위하여 인진호(A. capillaris)를 6종의 용매($dH_2O$, ethanol, methanol, acetone, ethyl acetate and hexane)로 추출한 추출물의 항산화 및 항아토피 활성을 검증하였다. 항산화 활성 검증에서는 methanol (DPPH: 85.87%, FRAP: 1.772)과 $dH_2O$ (DPPH: 60.69%, FRAP: 3.185) 등의 극성 용매 추출물에서 우수한 항산화 활성을 나타내었으나 비극성 용매에서는 활성이 거의 없었다(ethyl acetate 41.81%, 0.407, Hexane 8.37%, 0.328). DNFB-induced atopic dermatitis mouse model에 의한 항 아토피에 대한 치유력 측정에서는 인진호 추출물 처리군은 염증 세포의 수에서 음성 대조군과 비교하여 220% 증가로 양성 대조군의 400% 보다 약 45%의 완화 효과를 보여 주었으며, 귀의 두께는 음성 대조군의 175%로 양성 대조군의 330%보다 약 47%가 감소하는 뚜렷한 증상 완화 효과를 나타내었다. 이러한 결과는 인진호 추출물의 항산화 물질이 극성 물질이며, DNFB 처치에 의한 귀의 비후반응 감소와 염증부위의 면역세포 침투현상을 감소시키는 염증반응 완화 기능성 물질을 포함하고 있을 가능성을 알 수 있었다. 이상의 결과를 바탕으로 인진호 추출물에 대한 물리적 특성, 유전자 발현과 면역학적 실험 등이 추가 된다면 새로운 향장소재나 기능성 식품의 소재로도 이용 가능할 것으로 판단된다.

• 한국동물위생학회지
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• 제38권2호
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• pp.83-87
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• 2015

The exposure to house dust mites is closely connected with allergic diseases such as atopic dermatitis, allergic rhinitis and asthma. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. Therefore, repelling the house dust mites is more effective method than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus oils are regarded as safe compounds, there have been attempts to commercialize and market the insecticides/repellent products containing Eucalyptus oil as such or based upon them. For that reason, we experimented repellent activity against these mites using laundry detergent with Eucalyptus oil in this experiment. House dust mites (Dermatophagoides farinae and Dermatophagoides pteronyssinus) were exposed at different concentrations (4, 8, 16, $32{\mu}L/mL$) in fabric treated laundry detergent with Eucalyptus oil for 24 hours. The repellent effects (%) of laundry detergent with Eucalyptus oil exposed at 4, 8, 16, and $32{\mu}L/mL$ were 48.16%, 74.62%, 52.67%, and 44.47%, respectively. These results suggest that laundry detergent with Eucalyptus oil have potential effect to repel the house dust mites.

• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1725-1728
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• 2007

Mast cells are key effector cells in the early phase allergic inflammation and in diverse immunological and pathological processes. In order to understand the effect on reduction of the anti-dinitrophenyl (DNP) IgE antibody-induced β-hexosaminidase release in RBL-2H3 rat mast cells, a novel series of 4-senecioyloxymethyl- 6,7-dimethoxycoumarins (SMDC) was prepared by reacting 4-chloromethyl-6,7-dimethoxycoumarin with various carboxylic acids. Compounds 8-11 with cyclic moiety such as phenyl, thiophenyl, pyridinyl, and furanyl group were found to inhibit-hexosaminidase release more potently (5.98-9.62 μM) than compounds 3- 7 and 12 with acyclic moiety (19.32-76.78 μM). Furthermore, compounds 8 and 9 inhibited IgE-induced ear swelling and significantly reduced systemic passive cutaneous anaphylaxis reaction in mice.

• 한방안이비인후피부과학회지
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• 제13권2호
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• pp.165-181
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• 2000

Sinichengpaeum has been widely used in the oriental medical treatment of nasal diseases. These studies were performed done to investigate the effect of Sinicheng- paeum on the anti-allergic action We studied the vascular permeability response induced by the histamine and serotonin injection(allergy Ⅰ type), homologous PCA provoked by the IgE-like antibody against EWA(allergy Ⅳ type), contact dermatitis induced by picryl chloride(allergy Ⅳ type), delayed type hypersensitivity response to SRBC (allergy Ⅳ type), and the amount of eosinophil and IgE. The results were as follows: 1. The effect of Sinichengpaeum on vascular permeability responses to intradermal histamine and serotonin were significant. 2. In the homologous PCA provoked by the IgE-like antibody against white egg albumin, Sinichengpaeum showed a significant effect. 3. In the delayed type hypersensitivity responses to picryl chloride, Sini-chengpaeum provoked a significant effect. 4. After reaction provoked by picryl chloride, Sinichengpaeum showed an insignficant effect on amount of eosinophil, but a significant effect in IgE amount. 5. In the delayed type hypersensitivity responses to SRBC, Sinichengpaeum provoked a significant effect. 6. After reaction provoked by SRBC, Sinichengpaeum showed a significant effect on amount of cosinophil, but an insignificant effect in IgE amount.

• Toxicological Research
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• 제34권4호
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• pp.355-361
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• 2018

Methylisothiazolinone (MIT) has been used in combination with methylchloroisothiazolinone (CMIT) for cosmetic products such as shampoo, body lotion, and skin care products. The mixture of CMIT/MIT has been found to cause allergic contact dermatitis and is thus no longer permitted for use as a preservative in leave-on cosmetics. However, MIT itself was approved as a stand-alone preservative at a maximum concentration of 100 ppm as the toxicity was derived from CMIT rather than MIT. However, in many countries, allergic skin irritation caused by MIT remains a social concern. In this study, skin irritation was assessed for the presence of MIT, propylene glycol, and their mixture using a 3D human skin model $EpiDerm^{TM}$. Although non-diluted MIT causes serious skin toxicity, skin irritation was not observed at a concentration of 100 ppm, the maximum permissible level for cosmetics and personal care products according to European regulations. Propylene glycol, the most widely used vehicle for MIT, did not cause skin irritation in the 3D skin model. The results are expected to provide information for regulatory policies and guidelines on the use of biocides in consumer products.