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Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis

  • Park, So-Young;Yeung, Edward C.;Paek, Kee-Yoeup
    • Plant Biotechnology Reports
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    • v.4 no.4
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    • pp.303-309
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    • 2010
  • Endoreduplication is a developmental process that is unique to plants and occurs in all plants. The present study aimed to assess endoreduplication in various explant tissues and regenerated somatic embryos of Doritaenopsis. We further investigated the effects of light quality on endoreduplication and somatic embryo proliferation. To this end, we studied endoreduplication in leaves and root tips from regenerated plantlets and somatic embryos and in developing somatic embryos under 4 types of lighting conditions: red light, red + far-red light, red + blue light, and white light. We found that the degree of endoreduplication varied in different explants, and that the choice of explants used also influenced the ploidy levels of the newly regenerated somatic embryos. The DNA content of the leaf (2C-8C) was less than that of the root tip (2C-16C) and somatic embryo (2C-64C). In terms of light quality, the combination of red and far-red light produced the highest number of somatic embryos, while maintaining a low degree of endoreduplication. The data obtained indicate that this light combination stimulates somatic embryogenesis in Doritaenopsis and may exert some control on endoreduplication during cell division. These findings can be applied to achieve a reduction in somaclonal variations for the purpose of mass proliferation and genetic improvement.

Korean Red Ginseng extract ameliorates melanogenesis in humans and induces antiphotoaging effects in ultraviolet B-irradiated hairless mice

  • Saba, Evelyn;Kim, Seung-Hyung;Lee, Yuan Yee;Park, Chae-Kyu;Oh, Jae-Wook;Kim, Tae-Hwan;Kim, Hyun-Kyoung;Roh, Seong-Soo;Rhee, Man Hee
    • Journal of Ginseng Research
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    • v.44 no.3
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    • pp.496-505
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    • 2020
  • Background: Panax ginseng is a marvelous herbal remedy for all ailments of body. That may be why it is called Panax, which means "cure for all". Melanin is a pigment that gives color to our skin; however, increased melanin production can lead to tumor formation. Human exposure to ultraviolet B radiation has increased extensively owing to the increased sunlight due to global warming. Consequently, a phenomenon called photoaging has been observed for all skin colors and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases, which serve as degradation enzymes for extracellular matrix proteins, mainly collagen, is increased, causing depletion of collagen and resulting in early wrinkle formation. Methods: Therefore, in our study, we used the murine melanoma cell line B16/F10 to study the inhibition of melanogenesis by Korean Red Ginseng (KRG) extract in vitro and HRM-2 hairless mice exposed to artificial ultraviolet B to examine the efficacy of KRG in vivo. We prepared a 3% red ginseng extract cream and evaluated its effects on human skin. Results: Our results demonstrated that KRG induced potent suppression of tyrosinase activity and melanin production in B16/F10 cells; moreover, it reduced the transcription and translation of components involved in the melanin production pathway. In the in vivo experiments, KRG potently suppressed the expression of matrix metalloproteinases, reduced wrinkle formation, and inhibited collagen degradation. On human skin, ginseng cream increased skin resilience and skin moisture and enhanced skin tone. Conclusion: Therefore, we conclude that KRG is an excellent skin whitening and antiaging product.

Vegetative Compatibility Grouping and Pathogenicity of Colletotrichum gloeosporioides Isolates from Different Host Plants

  • Ahn, Il-Pyung;Kim, Soonok;Im, Kyung-Hwan;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.19 no.6
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    • pp.269-273
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    • 2003
  • A total of 57 isolates of Colletotrichum gloeosporioides were recovered from diseased tissues of Hall's crab apple (Malus haliana), 3 cultivars of edible apple (M. pumila var. dulcissima), red pepper (Capsicum annum), and grapevine (Vitis vinifera) fruits. All isolates showed strong virulence on their own host plants. Isolates from edible apple exhibited high level of cultivar specificity in pathogenicity tests. Ten isolates from apple cultivar 'Fuji' were virulent on 'Jonathan' and 'Rall's Genet'. However, 12 isolates from 'Jonathan' and 'Rall's Genet' were not virulent on 'Fuji'. Among the 24 isolates from red pepper, only seven and two isolates were infective on edible apple and grapevine fruits, respectively. All six isolates from grapevine were only virulent on their own host. These isolates were grouped into five vegetative compatibility groups (VCGs), A, B, C, D, and E, by demonstrating heterokaryosis through complementation using nitrate-nonutilizing (nit) mutants. Among them, isolates belong to VCG-A and VCG-D accounted for 24 and 17 isolates; those in VCG-A exhibited wide host range involving Hall's crab apple, all three edible apple cultivars, and red pepper. On the other hand, isolates of VCG-D and VCG-E showed limited host range specific to red pepper and grapevine, respectively. Taken together, the data suggest that among C. gloeosporioides isolates, the concepts of pathotype and/or forma specialis may exist, and that three is a relationship between host specificity and VCG grouping among C. gloeosporioides isolates.

The protective effect of Red Giseng on the organotin compounds(TBTO) poison : Focusing on the Immunity and sex hormone (유기주석화합물의 독성에 대한 홍삼의 방어효과 - 면역 및 성 호르몬물질 중심으로 -)

  • 최한영
    • Journal of environmental and Sanitary engineering
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    • v.16 no.3
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    • pp.8-13
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    • 2001
  • This study was carried out effect of red ginseng extract(1.0g/kg) on organotin compounds (10, 20 and 40 mg/kg) which poisons against some organs like thyroid gland, liver, kindey, testis, ovary, serum immuinty and sex hormone activity of rats were examinned by gastric tubing for 3 weeks. The weight of each organ in TBTO treated group were significantly increased other organs which excepted kinedy in males and only liver in females.(p<0.05, p<0.01). In case of Immunity activity of each sex, IgM level was small change comparsion with that of control group in all sex. but IgG level was significantly decreased females rather than males comparsion with that of control group.(p<0.05, p<0.01) In case of sex hormone activity, the testosterone activity of males and the estradiol activity of females were significantly decreased rather than the control group. on the other hand, red singsong treated group was only significantly increased estradiol activity.( p<0.05, p<0.01)

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A Study on the Evaluation of Economic Value of the Gulf of Mexico Recreational Red Grouper Fishery (여행비용모형 분석을 통한 유어(遊漁)활동의 경제적 가치 추정 -미국 멕시코만 Red Grouper 유어부문을 대상으로 -)

  • Kim, Do-Hoon
    • The Journal of Fisheries Business Administration
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    • v.36 no.2 s.68
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    • pp.121-134
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    • 2005
  • In order to evaluate the effectiveness of management measures and to provide policy suggestions for the allocation of total allowable catch between recreational and commercial sectors, the economic value of red grouper recreational fishery in the United States Gulf of Mexico was estimated using a Travel Cost Method(TCM), Due to the characteristic of count data, a Poisson model(PM) and a Negative binomial model(NBM) were used in the TCM. Results of models showed that the NBM was statistically more suitable than the PM since the overdispersion problem occurred in the PM. Results also indicated all signs of the estimated parameters were as expected and were significant, except for a Boat parameter in both models. Based on the results of NBM, the total economic value of the recreational red grouper fishery was estimated to be $\$698.6$ and the value per trip was $\$179.5$. In addition, the total changes in expected consumer surplus due to changes in catch rates was $ \$42.3$.

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Characteristics of Organic Acid of Makgeolli by Yeast Strains Type (효모의 종류에 따른 막걸리의 유기산 특성 연구)

  • Bang, Chan Mi;Moon, Joon-Kwan;Kong, Hong Sik
    • Korean journal of food and cookery science
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    • v.32 no.1
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    • pp.44-49
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    • 2016
  • Organic acid fermentation by yeast an important in the flavor and aroma. June 19th brewing each by each of the kinds of yeast (Instaferm, Instaferm red, La parisienne, La parisienne red, Fermivin, Safbrew wb-06, Safele s-04, Song chun) were analyzed organic acid during storage life. All yeast with reduced organic acid on 4 days. On 10 days, Instaferm, Instaferm red, La parisienne, La parisienne red, Fermivin yeast with the increased organic acid but on 19 days these reduced organic acid and maintain to 28 days. However use of Safbrew wb-06, Safele s-04, Song chun yeast the organic acid at storage and maintain organic acid content to 28 days. The variation of each organic acid during storage life characteristic of the yeast.

고추씨의 영양적 가치 평가와 사료 내 첨가가 산란계의 생산성에 미치는 영향

  • Chang, Ik-Hun;Roh, Seong-Rae;Kwon, Sun-Gwan;Kim, Eun-Jip;Lim, Ho-Jung;Kang, Chang-Won
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2001.11a
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    • pp.54-55
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    • 2001
  • The experiments were conducted to evaluate the feeding values of red pepper seed (Exp. 1) and to investigate its dietary supplementation on layer performances (Exp. 2). The values of TME, TMEn and average TAAA determined by force-feeding 14 roosters (Hy-line) were 2.83 kcal/g, 2.94 kcal/g and 82.72%, respectively. a total of 200 layers (ISA-brown) was divided into 4 groups with 5 replicates of 10 birds per replicate, and fed the experimental diets containing different level of red pepper seed (0, 3, 5, 10%). There were no differences in performances of laying hens among treatments. Liver lipids in all groups containing red pepper seed were significantly reduced as compared to those of control (P<0.05).

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INHIBITORY EFFECTS OF RED GINSENG EXTRACT ON PRENEOPLASTIC NODULE FORMATION IN THE RAT LIVER OBSERVED BY RAPID BIOASSAY OF CARCINOGENESIS

  • Kim, Yun-Bae;Lee, Yong-Soon
    • Toxicological Research
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    • v.4 no.2
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    • pp.159-179
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    • 1988
  • This study was carried out to investigate the inhibitory effects of red ginseng extract on preneoplastic nodule formation in the rat liver using a rapid bioassay of hepatocarcinogenesis. Wistar male rats in test groups were fed diets containing 3.0, 0.3 or 003% of red genseng extract for 6 weeks after 2 week of dosing with diethylnitrosamine (DEAN, 200 mg/kg B.W., I.P.) or saline solution. The rats in the control groups were treated with DENA or saline alone. Three weeks after the beginning of the experiment, the two-thirds partial hepatectomy was performed on all animals. The inhibitory effects were investigated by measuring the number, total area and size of hyperplastic nodules enhanced by the 2/3 partial hepatectomy following induction with DENA. The results obtained were summarized as follows: 1. In general, the body weight gains of the rats fed diets containing red ginseng extract were higher than those of other groups.

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Production of Red Pigments by Monascus purpureus in Solid-state Culture

  • Lee Bum-Kyu;Piao Hai Yan;Chung Wook-Jin
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.1
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    • pp.21-25
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    • 2002
  • To maximize and sustain the productivity of Monascus pigments, various environmental and nutritional parameters, such as the initial moisture content, pH, inoculum size, sample size, and nutrient supplement, that influence pigment production were evaluated in solid-state cultures as follows: initial moisture content, $50\%;$ pH, 6.0; inoculum size $1\;\times\;10^4$ spore cells $(grams\;of\;dry\;solid\;substrate)^{-1};$ sample size, 300 g. All supplementary nutrients (carbon, nitrogen, and mineral sources) added has inhibitory effects on the cell growth and red pigment production. In open tray culture the maximum biomass yield and specific productivity of red pigments were 223 mg DCW $(grams\;of\;initial\;dry\;substrate)^{-1}$ and, $47.6\;OD_{500}\;(DCW\;grams)^{-1}h^h{-1}$ respectively.

Effect of Controlled Atmosphere and Modified Atmosphere Storage on the Fatty Acid of Fresh and Red Ginseng (수삼의 CA 및 MA 저장이 홍삼의 지방산에 미치는 영향)

  • 전병선;최강주
    • Journal of Ginseng Research
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    • v.19 no.3
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    • pp.260-266
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    • 1995
  • Fifteen free fatty acids including myristic acid were isolated and identified from red ginseng processed from CA and MA stored fresh ginseng. Linoleic acid (80%) and palmitic acid (10.5%) content were the major components accounting for more than 90% of the total free fatty acid. The contents of free and identified fatty acids were not greatly changed in all the treatments. Especially when preservative was treated, the change of free fatty acid was quite stable over the control. Each fraction of neutral, glyco- and phospholipid was constant in terms of quantities before and after the treatment. Key words Controlled atmosphere, modified atmosphere, fresh ginseng, red ginseng, fatty acid.

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