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SNP discovery and applications in Brassica napus

  • Hayward, Alice;Mason, Annaliese S.;Dalton-Morgan, Jessica;Zander, Manuel;Edwards, David;Batley, Jacqueline
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.49-61
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    • 2012
  • This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

A Study on the Stereoscopic 3D Filmmaking Curriculum in the Film and Image Major (영화 영상 전공에서의 스테레오스코픽 3D 제작 교육 과정 연구)

  • Lee, Chan-Bok
    • The Journal of the Korea Contents Association
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    • v.10 no.6
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    • pp.222-235
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    • 2010
  • After its remarkable success, "Avatar" brought another way of creating cinematic story; the stereoscopic 3-D cinema. Comparing conventional 2-D filmmaking, you need twice as much of budget and manpower in 3-D filmmaking because of the complicated process and slow production speed. The 3-D hardware like 3-D TV and 3-D projector are already showing at the retail stores while 3-D filmmaking is still in veil, and no major educational institution is yet to start 3-D related education. As 3-D movies get popular and demand more 3-D filmmaking professional crew, educating 3-D filmmaking to 2-D based film students will improve their hiring rate in the market. The successful result of the box office showing 3-D films like "Alice in Wonderland 3D" and "Titan 3D" forecasts that there will be more demand on 3-D related jobs very soon.

Survival of Triple Negative versus Triple Positive Breast Cancers: Comparison and Contrast

  • Negi, Preety;Kingsley, Pamela Alice;Jain, Kunal;Sachdeva, Jaineet;Srivastava, Himanshu;Marcus, Sudeep;Pannu, Aman
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.8
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    • pp.3911-3916
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    • 2016
  • Background: Triple negative (TN) and triple positive (TP) breast cancers both are aggressive types but TN generally has a shorter survival. Objectives: To compare the clinical characteristics and treatment outcomes for patients with TN versus TP breast cancer and to assess various prognostic factors affecting overall survival. Materials and Methods: A retrospective audit of 85 breast cancer patients was conducted in the Department of Radiation Oncology and Medical Oncology on patients from 2006 to 2013 for whom IHC for ER, PgR and Her-2 neu were available. The patients were stratified into: ER-, PR- and Her-2 neu- (Arm A, n=47) and ER+, PgR+ and Her-2 neu+ (Arm B, n=38). Results: TN subtype had higher numbers of premenopausal and advanced stage patients as compared to TP subtype. The locoregional recurrence (LRR) and distant metastatic rate was also higher in TN subtype but there was no definite pattern in both the arms. Among the prognostic factors, patients with premenopausal status and advanced stage in TN breast cancer had inferior survival (P=0.07) whereas for those with postmenopausal status and early stage there was no survival difference between the two arms. Conclusions: TN subtype tends to be more aggressive in terms of younger age and advanced stage at presentation, higher tumour grade, LRR and metastasis, suggesting need for future research efforts on providing aggressive treatment to these patients. We could attribute better outcome for TP subtype to receptor positivity enabling role of hormonal treatment and targeted therapy, although less number of patients received targeted therapy.

Use of Vitamin D3 and Its Metabolites in Broiler Chicken Feed on Performance, Bone Parameters and Meat Quality

  • Garcia, Ana Flavia Quiles Marques;Murakami, Alice Eiko;Do Amaral Duarte, Cristiane Regina;Ospina Rojas, Ivan Camilo;Picoli, Karla Paola;Puzotti, Maira Mangili
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.3
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    • pp.408-415
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    • 2013
  • The objective of this experiment was to assess the use of different vitamin D metabolites in the feed of broiler chickens and the effects of the metabolites on performance, bone parameters and meat quality. A total of 952 one-day-old male broiler chicks were distributed in a completely randomised design, with four treatments, seven replicates and 34 birds per experimental unit. The treatments consisted of four different sources of vitamin D included in the diet, $D_3$, $25(OH)D_3$, $1,25(OH)_2D_3$, and $1{\alpha}(OH)D_3$, providing 2000 and 1600 IU of vitamin D in the starter (1 to 21 d) and growth phases (22 to 42 d), respectively. Mean weight, feed:gain and weight gain throughout the rearing period were less in animals fed $1{\alpha}(OH)D_3$ when compared with the other treatments (p<0.05). No significant differences were noted among the treatments (p>0.05) for various bone parameters. Meat colour differed among the treatments (p>0.05). All of the metabolites used in the diets, with the exception of $1{\alpha}(OH)D_3$, can be used for broiler chickens without problems for performance and bone quality, however, some aspects of meat quality were affected.

Sunflower cake with or without enzymatic complex for broiler chickens feeding

  • Berwanger, Eveline;Nunes, Ricardo Vianna;Pasquetti, Tiago Junior;Murakami, Alice Eiko;Oliveira, Taciana Maria Moraes de;Bayerle, Douglas Fernando;Frank, Rafael
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.410-416
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    • 2017
  • Objective: This study was to evaluate the sunflower cake and enzymatic complex fed to broilers from 22 to 42 d of age. Methods: In a completely randomized design, a total of 850 birds were allotted in a $2{\times}5$ factorial scheme (with and without enzymatic complex) and five inclusion levels (0%, 5%, 10%, 15%, 20%) of sunflower cake. There were 5 replications and 17 birds in each experimental unit. Data from performance, carcass yield and intestinal morphology were evaluated. Results: Feed intake, weight gain, final weight and feed:gain ratio linearly worsened as sunflower cake increased. For weight gain, final weight and feed:gain ratio, the birds whose diets contained levels of 15% and 20% of sunflower cake showed worse values (p<0.05) than the birds fed the control diet. When fed the enzymatic complex, birds improved (p<0.05) crypt depth and villus:crypt ratio in the jejunum. As inclusion levels of sunflower cake increased, villus depth and villus:crypt ratio in duodenum, jejunum and ileum linearly reduced and the crypt depth linearly increased. Carcass yield linearly reduced as sunflower cake increased. Conclusion: Based on performance, sunflower cake can be used up to 10% in broilers feeding from 21 to 42 days of age.

Statistical Applications for the Prediction of White Hispanic Breast Cancer Survival

  • Khan, Hafiz Mohammad Rafiqullah;Saxena, Anshul;Gabbidon, Kemesha;Ross, Elizabeth;Shrestha, Alice
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.14
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    • pp.5571-5575
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    • 2014
  • Background: The ability to predict the survival time of breast cancer patients is important because of the potential high morbidity and mortality associated with the disease. To develop a predictive inference for determining the survival of breast cancer patients, we applied a novel Bayesian method. In this paper, we propose the development of a databased statistical probability model and application of the Bayesian method to predict future survival times for White Hispanic female breast cancer patients, diagnosed in the US during 1973-2009. Materials and Methods: A stratified random sample of White Hispanic female patient survival data was selected from the Surveillance Epidemiology and End Results (SEER) database to derive statistical probability models. Four were considered to identify the best-fit model. We used three standard model-building criteria, which included Akaike Information Criteria (AIC), Bayesian Information Criteria (BIC), and Deviance Information Criteria (DIC) to measure the goodness of fit. Furthermore, the Bayesian method was used to derive future survival inferences for survival times. Results: The highest number of White Hispanic female breast cancer patients in this sample was from New Mexico and the lowest from Hawaii. The mean (SD) age at diagnosis (years) was 58.2 (14.2). The mean (SD) of survival time (months) for White Hispanic females was 72.7 (32.2). We found that the exponentiated Weibull model best fit the survival times compared to other widely known statistical probability models. The predictive inference for future survival times is presented using the Bayesian method. Conclusions: The findings are significant for treatment planning and health-care cost allocation. They should also contribute to further research on breast cancer survival issues.

Kuroshio Observation Program: Towards Real-Time Monitoring the Japanese Coastal Waters

  • Ostrovskii, Alexander;Kaneko, Arata;Stuart-Menteth, Alice;Takeuchi, Kensuke;Yamagata, Toshio;Park, Jae-Hun;Zhu, Xiao Hua;Gohda, Noriaki;Ichikawa, Hiroshi;Ichikawa, Kaoru;Isobe, Atsuhiko;Konda, Masanori;Umatani, Shin-Ichiro
    • Ocean and Polar Research
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    • v.23 no.2
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    • pp.141-160
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    • 2001
  • The challenge of predicting the Japanese coastal ocean motivated Frontier Observational Research System for Global Change (FORSGC) and the Japan Marine Science and Technology Center (JAMSTEC) to start a multiyear observational programme in the upstream Kuroshio in November 2000. This field effort, the Kuroshio Observation Program (KOP), should enable us to determine the barotropic and baroclinic components of the western boundary current system, thus, to better understand interactions of the currents with mesoscale eddies, the Kuroshio instabilities, and path bimodality. We, then, will be able to improve modeling predictability of the mesoscale, seasonal, and inter-annual processes in the midstream Kuroshio near the Japanese main islands by using this knowledge. The KOP is focused on an enhanced regional coverage of the sea surface height variability and the baroclinic structure of the mainstream Kuroshio in the East China Sea, the Ryukyu Current east of the Ryukyu's, and the Kuroshio recirculation. An attractive approach of the KOP is a development of a new data acquisition system via acoustic telemetry of the observational data. The monitoring system will provide observations for assimilation into extensive numerical models of the ocean circulation, targeting the real-time monitoring of the Japanese coastal waters.

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Route-optimized Handoff in Mobile CORBA Environment (Mobile CORBA 환경에서 게이트웨이간의 경로최적화 핸드오프)

  • Shin, Hye-Ryung;Lee, Hyung-Woo;Kim, Ju-Ho
    • Journal of KIISE:Information Networking
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    • v.29 no.3
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    • pp.224-232
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    • 2002
  • The routing protocols designed for wired networks can hardly be used for mobile ad-hoc networks due to limited bandwidth of wireless transmission and unpredictable topological change. Recently, several routing protocols for mobile ad-hoc networks have been proposed. However, when these protocols are applied to support real time services like multimedia transmission, they still have problems in ad-hoe networks, where the topology changes drastically. In this paper, we propose a new route selection algorithm which selects the most reliable rouse that is impervious to route failures by topological changes by mobile hosts. For reliable route selection, the concept of virtual zone (stable lone and caution zone) is proposed. The lone is located in a mobile node's transmission range and determined by mobile node's mobility information received by Global Positioning System (GPS). The proposed algorithm is applied to the route discovery procedure of the existing on-demand routing protocol, AODV, and evaluated by simulation in various traffic conditions and mobility patterns.

Identifying psychological predictors of adherence to a community-based lifestyle modification program for weight loss among Chinese overweight and obese adults

  • Leung, Alice Wai Yi;Chan, Ruth Suk Mei;Sea, Mandy Man Mei;Woo, Jean
    • Nutrition Research and Practice
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    • v.13 no.5
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    • pp.415-424
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    • 2019
  • BACKGROUND/OBJECTIVES: Existing evidence on lifestyle modification programs for weight loss is limited by the high attrition rate of such programs. Identifying predictors of adherence to a lifestyle modification program could result in program improvement. However, little is known about behavior-specific adherence and its psychological predictors. This study aimed to examine the psychological predictors of adherence after one-month participation in a community-based lifestyle modification program among Chinese overweight and obese adults in Hong Kong. SUBJECTS/METHODS: A total of 205 Chinese overweight and obese adults aged $38.9{\pm}10.5years$ completed the study. Data were collected at baseline and after one month using self-reported questionnaires, which assessed knowledge (self-developed scale), motivation (Treatment Self-Regulation Questionnaire), stage of change (Stage of Exercise Scale) and self-efficacy (Self-Rated Abilities for Health Practices Scale). At one month, a 4-day dietary recall and the International Physical Activity Questionnaire-Short Form were used to assess dietary and physical activity (PA) adherence. Food and PA diaries were examined to indicate self-monitoring. Program attendance was tracked between baseline and one-month follow-up. RESULTS: After one month, participants reported high dietary adherence, attendance, and adherence to self-monitoring but low PA adherence. Multiple regression analyses suggested that diet self-efficacy (baseline) and nutrition knowledge (one-month change) were independent predictors of dietary adherence score at one month, whereas autonomous PA motivation (baseline) and PA self-efficacy (both baseline and one-month change) were independent predictors of PA adherence score at one month. No significant psychological predictor was identified for attendance or self-monitoring. CONCLUSIONS: The results suggest that the effect of psychological factors on adherence differs between diet and PA adherence outcomes. To promote adherence, practitioners should assess self-efficacy, knowledge, and motivation at the beginning of a weight-loss program and explore behavior-specific strategies to improve knowledge and self-efficacy. The results of this study have direct implications for program improvements.

Temporal and spatial variability in the nutritive value of pasture vegetation and supplement feedstuffs for domestic ruminants in Western Kenya

  • Onyango, Alice Anyango;Dickhoefer, Uta;Rufino, Mariana Cristina;Butterbach-Bahl, Klaus;Goopy, John Patrick
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.5
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    • pp.637-647
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    • 2019
  • Objective: The study aimed at quantifying seasonal and spatial variations in availability and nutritive value of herbaceous vegetation on native pastures and supplement feedstuffs for domestic ruminants in Western Kenya. Methods: Samples of herbaceous pasture vegetation (n = 75) and local supplement feedstuffs (n = 46) for cattle, sheep, and goats were collected in 20 villages of three geographic zones (Highlands, Mid-slopes, Lowlands) in Lower Nyando, Western Kenya, over four seasons of one year. Concentrations of dry matter (DM), crude ash (CA), ether extract (EE), crude protein (CP), neutral detergent fibre (NDF), gross energy (GE), and minerals were determined. Apparent total tract organic matter digestibility (dOM) was estimated from in vitro gas production and proximate nutrient concentrations or chemical composition alone using published prediction equations. Results: Nutrient, energy, and mineral concentrations were 52 to 168 g CA, 367 to 741 g NDF, 32 to 140 g CP, 6 to 45 g EE, 14.5 to 18.8 MJ GE, 7.0 to 54.2 g potassium, 0.01 to 0.47 g sodium, 136 to 1825 mg iron, and 0.07 to 0.52 mg selenium/kg DM. The dOM was 416 to 650 g/kg organic matter but differed depending on the estimation method. Nutritive value of pasture herbage was superior to most supplement feedstuffs, but its value strongly declined in the driest season. Biomass yields and concentrations of CP and potassium in pasture herbage were highest in the Highlands amongst the three zones. Conclusion: Availability and nutritive value of pasture herbage and supplement feedstuffs greatly vary between seasons and geographical zones, suggesting need for season- and region-specific feeding strategies. Local supplement feedstuffs partly compensate for nutritional deficiencies. However, equations to accurately predict dOM and improved knowledge on nutritional characteristics of tropical ruminant feedstuffs are needed to enhance livestock production in this and similar environments.