• Title/Summary/Keyword: Algicidal Activity

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Toxicity of Chloramine-T on the Flounder, Paralichthys olivaceus, and Bactertcidal Activity Against Fish Pathogenic Bacteria and Blue Green Algae, Tetraselmis suecica (Chloramine-T의 넙치에 대한 독성과 어병세균 및 Blue Green Algae에 대한 살균 효과)

  • Hwang, Eun-A;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.13 no.1
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    • pp.37-44
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    • 2000
  • CT(Sodium N-chloro-para toluenesulfonamide, Chloramine-T) known to be a strong oxidative agent was investigated to use as a candidate of disinfectant in the marine aquaculture industry by the analysis of the bactericidal activity against different aquatic microorganisms. One hundred percent mortality appeared at and above 16 ppm CT in sea water in flounder, the predominant species in the marine aquaculture of Korea, when exposed for 48hr. However, bactericidal activity was appeared to be very effective, and all different species of the fish pathogenic bacteria exposed to CT of less than 2 ppm in sea water were dead within 15 min. It allowed us to confirm that CT could be a very effective disinfectant to protect the spread of fish pathogenic bacteria derived from diseased fish or sea water in marine aquaculture. High concentration(10 ppm) with longer exposing time(24 hrs) was required for the algicidal activity of CT, at which concentration might induce the acute toxicity against fish, however, restricted the expanded use of CT for the elimination of zooplanktons at phytoplanktons in marine farms. Moreover the bactericidal activity of CT inhibited almost completely in the present of more than 10 ppm of organic materials pointed out that quality of the culturing sea water should be considered carefully for the application CT to the aquatic farms as a therapeutic agent.

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Algicidal Characteristics of 1-Alkyl-3-Methylimidazolium Chloride Ionic Liquids to Several Fresh-water Algae (이온성 액체 1-alkyl-3-methylimidazolium chloride계 화합물의 담수조류에 대한 살조활성 특징)

  • Hwang, Hyun-Jin;Kim, Jae-Deog;Choi, Jung-Sup;Kim, Young-Wun;Kim, Jin-Seog
    • Korean Journal of Weed Science
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    • v.30 no.3
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    • pp.233-242
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    • 2010
  • This study was conducted to know that if ionic liquids can be applicable as control agents of harmful algae in water-ecosystem and to find out problems caused by ionic liquid application. Firstly, the differential selectivity of various fresh-water algal species to several 1-alkyl-3-methylimidazolium chloride ionic liquids was investigated. There was a distinct differential response between alkyl chain lengths from butyl to dodecyl and towards the algal organisms : Generally algicidal activity was increased with increase of chain length and among the algae used in this study, Stephanodiscus hantzschii f. tenuis, Oscillatoria tenuis and Spirulina pratensis were most sensitive to 1-dodecyl-3-methylimidazolium chloride (MAIC12), next was Microcystis aeruginosa, and the others were relatively less sensitive to the chemical. The selectivity degree was about ten to twenty times based on the $EC_{80}$ (Effective concentration required for 80% growth inhibition). Secondly, an activity persistence of ionic liquids was investigated in natural mimic condition (using water bottle containing soil-sediments under the greenhouse condition). At the application of $1.0{\mu}g\;mL^{-1}$ of 1-octyl-3-methylimidazolium chloride (MAIC8), the algal growth did not occur at all until 6 days after treatment(DAT) and observed a only little growth at 9 DAT. But the algae grew rapidly after 9 DAT. So at 20 DAT, total chlorophylls was $264.4{\mu}g\;L^{-1}$ and the growth was inhibited by 58.2% compared to untreatment. On the other hand, MAIC12 also had a similar persistence pattern to MAIC8, showing nearly 5 times more activity than MAIC8. At 20 days after $0.2{\mu}g\;mL^{-1}$ application of MAIC12, that is, total chlorophylls was $251.2{\mu}g\;L^{-1}$ and the growth was inhibited by 55.2% compared to untreatment. In summary, 1-alkyl-3-methylimidazolium chloride ionic liquids is likely to be applicable for selective control of harmful algae as potent compounds having long lasting activity. However, the difficulty of degradation seems to be a limiting factor in an eco-friendly application of the compounds.

A Simple Screening Method for Anti-attachment Compounds Using Monospores of Porphyra yezoensis Ueda

  • Choi Jae-Suk;Kang Se-Eun;Cho Ji-Young;Shin Hyun-Woung;Hong Yong-Ki
    • Fisheries and Aquatic Sciences
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    • v.8 no.2
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    • pp.51-55
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    • 2005
  • We measured the anti-attachment activity of allelochemical and antifouling substances using monospores from Porphyra yezoensis Ueda as an assay. Methanol or aqueous extracts ($20/{\mu}g/mL$) from 32 seaweeds were added to monospore suspensions. Methanol extracts of Corallina pilulifera, Ishige sinicola, Sargassum horneri, and Sargassum sagamianum inhibited attachment $by\;>90\%$ compared to the reference. Phenolic compounds fractionated from S. sagamianum caused the most potent inhibition. P. yezoensis monospores also showed significant sensitivity to known allelochemical and algicidal compounds.

Histological Effect of Sodium Hypochlorite (NaOCl), Exposed at Red Tide-killing Concentrations, in Rockfish and Little Neck Clam (적조생물구제농도의 Sodium Hypochlorite(NaOCl)의 노출에 따른 조피볼락 및 바지락의 조직학적 영향)

  • 한조희;김영석;허민도;정해진;박관하
    • Environmental Analysis Health and Toxicology
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    • v.16 no.2
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    • pp.67-74
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    • 2001
  • In a previous study by Kim et al. (2000), sodium hypochlorite (NaOCl) has been demonstrated to be effective against algae that cause red tides. To secure the environmental safely of the chemical in practical use, effect of NaOCl, at concentrations required for algicidal activity, on the histology of rockfish and little neck clam was examined. When the animals were exposed to NaOCl at concentrations of 0.5 or 2ppm for 1 hr, there was no exposure-associated histological change in either animal. As the experimental exposure condition was set in consideration of the use, our results provide safety information necessary for practical application to marine fields.

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Effect of photosensitizer aganist anti-red tide substances from korea seaweed extracts

  • Jin, Hyung-joo;Jin, Long-Guo;Hong, Sung-Youl;Hong, Yong-Ki
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.153-154
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    • 2000
  • Many bioactive photosensitizers have been isolated from plants and some of theses possess antiviral activities which, depend on UVA(320-400nm) and visible light. Many complex quinonoids have been isolated from plants and recently some of them, such as hypericin and hypocrellin, have been shown to possess light-dependent antiviral activities. these compounds only exert their desired biological activities in the presence of long wavelength ultra-violet UVA but these activities are also strongly influenced b chemical structure(Hudson et al, 1995). the importance of light in the use of certain medicinal plant extracts has been appreciated, if not understood, for centuries. Also, many seaweed extracts will effect photo(UVA, visible light) for algicidal activity. (omitted)

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Statistical Optimization of Medium Components for the Production of Prodigiosin by Hahella chejuensis KCTC 2396

  • Kim, Sung-Jin;Lee, Hong-Kum;Yim, Joung-Han
    • Journal of Microbiology and Biotechnology
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    • v.18 no.12
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    • pp.1903-1907
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    • 2008
  • Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin production by Hahella chejuensis KCTC 2396, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among 12 components included in basal medium, $NaHCO_3$, ${Na}_{2}{SiO}_{3}$, ${NH_4}{NO_3}$, ${Na}_{2}{SO}_{4}$ and $CaCl_2$ were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: 1% sucrose; 0.4% peptone; 0.1 % yeast extract; and (g/l): NaCl, 20.0; ${Na}_{2}{SO}_{4}$, 9.0; $CaCl_2$, 1.71; KCl, 0.4; and (mg/l): ${H_3}{BO_3}$, 10.0; KBr, 50.0; NaF, 2.0; $NaHCO_3$, 45.0; ${Na}_{2}{SiO}_{3}$, 4.5; ${NH_4}{NO_3}$, 4.5. The predicted maximum yield of prodigiosin in the optimized medium was 1.198 g/l by the Box-Behnken design, whereas the practical production was 1.495 g/l, which was three times higher than the basal medium (0.492 g/l).

Isolation of Bacterial Strains Inhibiting the Growth of Microcystis aeruginosa and Cyanobacterium Growth Inhibition Assay (녹조 원인 남세균 Microcystis aeruginosa의 생장을 억제하는 세균균주의 분리 및 남세균 생장 억제능 검정)

  • Chung, Seon-Yong;Ko, Joon-IL;Kwon, Bum-Gun;Salma, Umme
    • Journal of Wetlands Research
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    • v.19 no.4
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    • pp.443-450
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    • 2017
  • The objective of this study was to isolate algae growth inhibiting microorganism to biologically control Microcystis aeruginosa, which is a harmful cyanobacterium. Various bacterial strains were isolated in this study, and four bacterial strains of M1~M4 exhibited remarkable growth inhibiting activity against M. aeruginosa. Based on the 16S rRNA analysis, the isolated M1~M4 strains were identified, and isolated four strains were rod-type and gram-negative. In particular, as well as respective single strain, co-culture of the isolated M1~M4 strains showed obvious algicidal activity against M. aeruginosa. When mixed four strains were inoculated, about 50% of the chlorophyll a was reduced after two days, about 70% after four days, and about 80% after seven days. From these results mentioned above, the four bacterial strains may contribute to the control of harmful M. aeruginosa.

Characteristics of the Algal Growth inhibition Substances Produced by Alteromonas sp. SR-14 (Alteromonas sp. SR-14가 생산하는 조류증식 저해 물질의 특성)

  • 김지회;이희정;이태식;김형락;이명숙;장독석
    • Journal of Food Hygiene and Safety
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    • v.14 no.3
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    • pp.270-276
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    • 1999
  • In previous reports, the authors isolated the algicidal marine bacterium, Alteromonas sp. SR-14 and demonstrated its growth inhibition of diatom, Chaetoceros calcitrans (C. calcitrans). In this paper, we studied the effects of cell free culture filtrate of Alteromonas sp. SR-14 on the growth of C. calcitrans, and the characteristics of the algal growth inhibition substance. The culture filtrate of Alteromonas sp. SR-14 grown in peptone broth showed growth inhibition activity against C. calcitrans. The reasonable culture conditions of the bacterium for producing of algal growth inhibition substances were $15~20^{\circ}$ in temperature, 7.0-9.0 in pH and $23~30{\textperthousand}$ in salinity, respectively. The algal growth inhibition activity of culture filtrate was increased from stationary phase in growth curve of Alteromonas sp. SR-14. The molecular weights of algal growth inhibition substances produced by Alteromonas sp. SR-14 were ranged about from 3 KDa to 12 KDa. Among the substances, less than 10 KDa fraction were stable by heating at $100^{\circ}$ for 10 minutes, while more than 10 KDa fraction were heat labile. According to the experimental results, the algal growth inhibition substance produced by the bacterium was not a single compound.

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Isolation and Characterization of the Marine Bacterium, Alteromonas sp. SR-14 Inhibiting the Growth of Diatom, Chaetoceros Species (규조류 Chaetoceros sp. 증식 저해균 Alteromonas sp. SR-14의 분리 및 특성)

  • KIM Ji Hoe;PARK Jeong Heum;SONG Young Hwan;CHANG Dong Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.2
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    • pp.155-159
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    • 1999
  • Marine bacteria inhibiting the growth of the diatom, Chaetoceros calcitrans were screened from seawater samples collected at south coast of Korea in 1996. Six strains were isolated from those samples. Among them, a bacterium SR-14 strain had the strongest inhibition activity against the alga. The selected SR-14 strain was identified as an Alteromonas sp. (supposed to be Alteromonas colwelliana) according to its biochemical results. Alteromonas sp. SR-14 was able to grow in raw seawater, aged seawater, Conwy medium for culture of microalgae and C. calcitrans culture filtrate. The host ranges of Alteromonas sp. SR-14 were C. calcitrans, C. muclleri and C. negracile among 10 species of diatom. All of the Chaetoceros spp. tested were inhibited by the Alteromonas sp. SR-14, However, the growth of the other genera in Bacillariophyceae was not inhibited.

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