• 원예과학기술지
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• 제32권6호
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• pp.901-905
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• 2014

The efficacy of ozone micro-bubble water (OMBW) in reducing microbial populations on alfalfa seeds was investigated in this study. We observed the surface of alfalfa seeds using microscopy and found that many cracks and crevices existing on the surface could harbor pathogens. Alfalfa seeds were treated with tap water (TW), micro-bubble water (MBW), ozone water (OW), ozone micro-bubble water (OMBW), and chlorine water (CL) for 5 min, and total microbial population, E. coli and Salmonella spp. colonies were determined. Also, the sterilized seeds were germinated and cultivated for 5 d after sowing to investigate the percentage of germination and the growth of alfalfa sprouts. The treatments with OMBW and CL were most effective in reducing total microbial populations and E. coli was eliminated by OW, OMBW, and CL treatments. CL treatment reduced the percentage of germination and fresh weight of alfalfa sprouts, but OMBW did not cause any negative effects on the germination and growth of alfalfa sprouts. These results indicate that OMBW can be used as an effective sanitizer for eliminating seed-borne pathogens without detrimental effects on seed viability.

• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.226-228
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• 2005

• Preventive Nutrition and Food Science
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• 제16권2호
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• pp.184-188
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• 2011

The melatonin, total polyphenol contents, and DPPH radical scavenging activity were determined in alfalfa, chicory, rape, red kale and sunflower after germination for four days at $24{\pm}0.1^{\circ}C$. Compared with seeds, melatonin content was increased in all sprouts, at the highest level in red kale (2,502.9 pg/g, 5.6 times higher than seed) followed by rape (2,430.1 pg/g), chicory (2,037.7 pg/g), alfalfa (1,160.8 pg/g) and sunflower (768.2 pg/g) sprout, however, the addition of tryptophan (0.5 mM), the precursor of melatonin synthesis, did not show any desirable effect. Both polyphenol content and DPPH radical scavenging activity were substantially increased in chicory (8.7 mg/g, 66%), rape (10.7 mg/g, 51%) and red kale (11.0 mg/g, 53%) sprouts, but not in alfalfa and sunflower sprouts. Melatonin content per gram polyphenol (ng/g) was also increased in all sprouts through germination. Germination was effective in increasing melatonin in all seeds tested, while its effect on polyphenol content and DPPH radical scavenging activity was species dependent.

• 한국식품영양학회지
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• 제20권4호
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• pp.356-362
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• 2007

This study was carried to develop the new material of functional food which antioxidant of natural substances of sprouts. We compared antioxdiative activity and antioxidant substances exist in sprouts. Antioxidaive activities of sprouts were measured by total polyphenolic acid contents, electron donating activity(EDA), SOD-liked activity and hydroxyl radical scavenging ability. Total polyphenolic acid content was very higher in Rape sprouts extract(Rap) than other sprout extracts. Also Rape extract was showed the most excellent antioxdiative activity in SOD liked activity at 86.94%. The EDA was ordered Rape sprouts extract(Rap)>Tatsoi sprouts extract(Tat)>Broccoli sprouts extract(Bro)>Alfalfa sprout extract(Alf). Hydroxy radical scavenging ability was the most effective in Rape sprouts extract(Rap). Therefore we could be certain that Rape sprouts extract(Rap) was the most effective in antioxidative activity from sprouts.

• 생물환경조절학회지
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• 제16권4호
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• pp.415-419
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• 2007

저장중 5가지 싹채소, (무, 적무, 적양배추, 알팔파, 브로콜리)의 생체중은 $50{\mu}m$ LDPE필름으로 밀폐하여 $2^{\circ}C$와 $8^{\circ}C$모두 99%이상 유지되었다. 저장중 포장재내 이산화탄소 농도는 $8^{\circ}C$에서 $2^{\circ}C$에서 보다 높았으며 작물별로 무와 적무가 높았다. 산소농도는 이와 정반대로 $2^{\circ}C$에서 높게 유지되었고, 무와 적무가 가장 낮은 농도를 보였는데 특히 $8^{\circ}C$저장 3일째는 3%미만까지 낮아지기도 하였다. 에틸렌 농도도 $2^{\circ}C$에 비해 $8^{\circ}C$에서 다소 높았으며 작물별로는 알팔파가 0.1ppm으로 가장 낮았고 나머지 4 작물은 작물간 농도차이에 유의성 없이 1ppm미만으로 낮았다. 이취는 산소농도가 가장 낮았던 무와 적무에서 오히려 낮은 수준을 보였으며 온도별로 큰 차이가 없었다. 외관상 품질은 역시 $2^{\circ}C$가 $8^{\circ}C$보다 높게 유지되었으며, 두 온도 모두에서 무가 가장 높게 유지되었고, 다음으로 적무, 브로콜리, 적양배추, 알팔파 순서였다. 이상의 결과에서 싹채소의 작물간 저장성에 차이를 알 수 있었는데 무와 알팔파의 경우 $4{\sim}5$일의 저장기한의 차이를 보였다. 따라서 싹채소 유통에 있어 작물별 관리가 다르게 실시되어야 할 것이라 생각된다.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.292-298
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• 2009

본 연구에서는 새싹종자 소독처리 여부에 따른 재배기간별 미생물 오염도 수준을 파악하고, 종자소독이 발아율에 미치는 영향을 분석하였다. 수입산 새싹종자 알팔파(alfalfa), 브로콜리(broccoli), 클로버(clover), 적양배추(red cabbage), 적무(red radish) 5종에 대하여 소독제 처리 여부에 따른 발아율과 재배기간별 미생물 분석을 실시하였다. 소독제 종류에 관계없이 새싹종자(알팔파, 클로버, 적무)는 48시간 후 90% 이상 높은 발아율을 보였다. 종자 소독 시 식중독균은 검출되지 않았고, 대조군에 비하여 유의하게 낮은 미생물 수준을 보였으나, 발아과정에서 일반세균, 대장균군 모두 $10^7-10^8CFU/g$으로 종자소독 여부에 관계없이 종자에 비하여 유의하게 높은 오염 수준을 나타내었다(p<0.05). 또한 종자 소독하지 않은 클로버에서 Listeria monocytogenes 이 검출되었다. 따라서 새싹채소의 위생적인 안전성을 개선하기 위하여 미국 FDA 권고에 따라 재배전 종자의 소독이 필요하며, 발아과정의 HACCP 관리계획을 마련하여 중점적으로 관리하는 것이 필요하겠다.

• 한국식품과학회지
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• 제51권3호
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• pp.207-213
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• 2019

This study was conducted to investigate the antioxidant contents and activities of twelve vegetable sprouts (broccoli, red radish, radish, mizuna, kale, taatsai, pak choi, Chinese cabbage, turnip, rapeseed, chicory, and alfalfa). The total flavonoid contents of the broccoli, red radish, and radish sprout were $25.36{\pm}0.13$, $25.26{\pm}1.80$, and $25.16{\pm}1.25mg$ CE/100 g FW, respectively, and were significantly higher than those of the other tested vegetables. Radish sprouts had the highest total phenolic content (112.42 mg GAE/100 g FW), followed by red radish and broccoli sprouts. The main polyphenols in the vegetable sprouts were epicatechin and chlorogenic acid, but they varied across sprout varieties. The correlation between total flavonoids and total phenolics for the 12 vegetable sprouts was very high (r=0.926). The total antioxidant activity (DPPH and ABTS radical scavenging activities) was also highly correlated with total flavonoids and total phenolics.

• Food Science and Biotechnology
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• 제16권2호
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• pp.171-176
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• 2007

Fresh sprouts such as alfalfa, mung bean, radish, broccoli, and soybean sprouts have become very popular due do their high nutritional value. However, there have been several outbreaks of illness in the last few years that have been attributed to sprout consumption. A number of methods have been used to improve the safety of seed sprouts. One promising technology is the use of ionizing radiation treatment. Irradiation with doses up to 8 kGy has been approved in the USA to control microbial pathogens in seeds intended for sprout production. This review focuses on the potential use of ionizing radiation in reducing the pathogen levels in seed sprouts. The effects of irradiation on seed germination and the nutritional quality of the sprouts are discussed.

• Food Science and Biotechnology
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• 제27권6호
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• pp.1865-1869
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• 2018

This study examined the effects of alfalfa seed germination on growth of Salmonella enterica. We investigated the population changes of S. enterica during early sprout development. We found that the population density of S. enterica, which was inoculated on alfalfa seeds was increased during sprout development under all experimental temperatures, whereas a significant reduction was observed when S. enterica was inoculated on fully germinated sprouts. To establish a model for predicting S. enterica growth during alfalfa sprout development, the kinetic growth data under isothermal conditions were collected and evaluated based on Baranyi model as a primary model for growth data. To elucidate the influence of temperature on S. enterica growth rates, three secondary models were compared and found that the Arrhenius-type model was more suitable than others. We believe that our model can be utilized to predict S. enterica behavior in alfalfa sprout and to conduct microbial risk assessments.

• 생명과학회지
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• 제19권6호
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• pp.751-755
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• 2009

본 연구는 새싹채소 중에서 알팔파싹, 브로콜리싹, 클로버싹 및 적무싹을 대상으로 초기 미생물오염도가 비교적 높은 원재료를 선별하여 염소수처리를 한 후 미생물적.관능적 품질을 평가하였다. 본 연구에서 사용된 알팔파싹, 브로콜리, 클로버, 및 적무 새싹채소의 미생물오염도는, 총균수 $10^{7}$ CFU/g 이상, 대장균군수 $10^{6}$ CFU/g 이상으로 높게 나타났고, 대장균수의 경우 알팔파싹과 클로버싹에서는 검출되지 않았으나 브로콜리싹과 적무싹에서는 $10^3{\sim}10^4$ CFU/g의 수준으로 검출되어 초기 미생물 오염 수준이 상당히 높은 것으로 확인되었다. 이와같이 초기오염도가 높은 새싹채소를 50, 100, 150 ppm의 염소수로 처리 후에 미생물적 품질을 평가한 결과, 100 ppm의 염소수 처리군에서 가장 낮은 세균수를 나타내어 살균 효과가 큰 것으로 확인되었다. 그리고 염소수의 침지시간은 5분간 처리군 보다는 60분 처리군에서 대조구 대비 $1.3{\sim}2.2$ log scale의 가장 큰 감균효과를 나타냈다. 그러나 클로버에서 $3.6{\times}10^{6}$ CFU/g의 총균수가 검출되었고 모든 처리군에서 $10^{4}$ CFU/g 이상의 대장균군이 검출되어 생야채의 미생물 안전 기준치를 초과하는 것으로 나타났다. 이는 새싹채소의 원재료 자체가 높은 미생물 오염이 높은경우에는 염소수처리에 의하여 $1{\sim}2$ log scales 정도의 감균효과를 나타내더라도 생야채의 안전 기준치에는 미치지 못하는 것으로 사료된다. 따라서 단체급식소의 식품 안전성을 확보하기 위해서는 생산 및 유통과정에서 새싹채소의 초기 미생물 오염도를 낮추는 것이 필수적이며, 아울러 새싹채소의 전처리 단계에서 미생물 제어에 효과적인 살균방법의 체계적인 연구가 지속되어야 할 것으로 사료된다.