• 대한본초학회지
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• 제34권1호
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• pp.67-74
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• 2019

Objectives : Alcohol hangover is a common phenomenon which basically occurs after heavy drinking. Moreover, heavy alcohol consumption leads to acute and chronic diseases. We investigated the effect of herbal mixture (SJ) on alcohol metabolism in serum or/and liver. Methods : 5W-old Sprague Dawley rats were used for the study. To overnight fasted rats, 0.9% saline or SJ extract was administrated per os before alcohol treatment. Then, 40% alcohol was orally administrated to all rats in 30 mins. Ethanol, acetaldehyde concentrations, alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH) activities were measured by assay kits. Aspartate aminotransferase (AST) and alanine transaminase (ALT) were measured by analyzer. Results : Ethanol and acetaldehyde concentrations lowered in SJ groups compared with CON group. Especially, acetaldehyde concentration significantly decreased in SJ groups compared with CON group. AST and ALT levels tended to increase in SJ groups compared with CON group but there was no significant difference between CON group and SJ groups. ADH activity in serum was higher in SJ groups than CON group but no significant difference in liver. ALDH activity in both serum and liver showed significantly increased in SJ groups compared with CON group. Conclusions : Treatment of SJ extract showed not only reducing concentration of alcohol and acetaldehyde but also increasing activities of ADH and ALDH. These results suggest SJ may influence in alcohol metabolism via control of metabolic enzyme activities and metabolite. Therefore, SJ, herbal mixture, might have a function of preventing hangover after drinking alcohol.

• BMB Reports
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• 제42권10호
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• pp.673-678
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• 2009

The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses.

• Journal of Microbiology
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• 제33권2호
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• pp.126-127
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• 1995

A Zymomonas mobilis DNA fragment consisting of 207 nucleotides, which corresponded to the 5'-flanking region of an adhB gene encoding alcohol dehydrogenase II, was fused to the structural gene coding for a Bacillus endo-.betha.--1, 4-glucanase. The Z. mobilis DNA framgment waw identified to promote 50-fold increase in the expression of endo-.betha.1. 4 glucanase gene in Escherichia coli.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.323-323
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• 1994

Allyl alcohol은 간에서 두 단계의 효소 반응을 거쳐 대사되는데, 먼저, alcohol dehydrogenase (ADH)에 의해 독성 활성체인 acrolein으로 바뀌고, 이후 계속하여 aldehyde dehydrogenase (ALDH)에 의해 acrylic acid로 무독화되어 배설된다. Ethanol 역시 간에서 대사되는데 있어 같은 효소들을 공유하므로 allyl alcohol과 경쟁적으로 반응할 것이다. 따라서, 본 실험에서는 ethanol에 의한 대사 효소 경쟁반응에 의해 allyl alcohol 의 간독성이 어떻게 변화하는지를 연구하였다. 우선 ethanol과 allyl alcohol을 동시 투여할 경우 5시간째에 allyl alcohol에 의해 증가된 ALT level을 낮춘다는 보고를 확인하고자 ethanol 2 g/kg과 allyl alcohol 40 mg/kg을 동시투여했으나 오히려 치사율이 증가했고, ethanol을 2시간 전처리한 군에서도 역시 치사율이 증가되고, 간의 glutathione 양은 allyl alcohol 단독 처리군에 비해 현저히 감소되는 양상을 보였다.

• 한국자원식물학회지
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• 제10권4호
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• pp.319-323
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• 1997

Spraque-Dawley계 응성흰쥐에 계통분획한 cornsilk의 각 분획물을 경구투여하고 혈청 Ethanol농도와 간의 Alcohol dehydrogenase활서에 미치는 효과를 검색 추적한 결과 알코올대사를 촉진시키는 성분은 주로 에탄올가용부에, 억제시키는 성분은 에탄올불용부에 주로 존재함을 추정할 수 있었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.335.2-335.2
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• 2002

Despite the use of antibiotics and vaccines, Streptococcus pneumoniae remains a serious cause of morbidity and morality in human. In this study, pathogenic and immunological natures of alcohol dehydrogenase (ADH) in S. pneumoniae were elucidated. In vitro cytotoxicity test determined by lactate dedydrogenase release from A549 cells revealed that adh mutation significantly reduced cytotoxicity although in vivo intraperitoneal challenge of the adh mutant to BALB/C mouse exhibited marginal increase of survival time than the wild type. (omitted)

• 한국작물학회지
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• 제53권4호
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• pp.440-446
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• 2008

상시담수 하에서 발아 및 안정적인 입모를 확보하는 것은 담수직파에서 매우 중요한 일이다. 일반적으로 담수 조건에 서는 유묘생장이 억제되지만, 담수 처리 시 초기생육이 우수한 익산429와 담수 처리 시 생육이 저조하며 생존율이 낮은 Woodrose 2품종에 대해 발아 및 초기생육기간 담수 처리하여 혐기대사와 관련된 ADH활성과 항산화효소들의 변화를 관찰하였다. 1. 담수 처리 시 벼는 유아만 신장하며 뿌리는 신장하지 못했고, 익산429가 Woodrose보다 담수상태에서 생존기간이 길었고 생장도 빨랐다. 그러나 무 처리에서는 Woodrose의 생육이 빨랐다. 2. ADH활성은 담수 처리 시 무 담수에 비해 급격히 증가하였고, 익산429가 Woodrose보다 활성이 오래 유지되었는데, 처리 7일째는 익산429가 무처리에 비해 약 7배, Woodrose는 약 2.5배 증가하였다. 4. SOD, POX, CAT 활성 모두 담수 처리에서 무 담수보다 낮았다. 그러나 SOD는 담수시 무 담수에 비해 크게 활성이 낮지는 않았으며 품종 간에는 익산429의 활성이 무 담수 및 담수 처리 모두 Woodrose보다 높았고 담수 시에도 익산429가 Woodrose보다 높게 유지되었다. POX 활성변화는 담수시 익산429가 지속적으로 증가한 반면 Woodrose는 큰 변화가 없었다. CAT활성은 담수 처리 초기부터 아주 낮은 활성을 보여 처리기간 동안 거의 증가하지 않았으나 무 담수시에는 3일까지 활성이 크게 증가하였다. 따라서 담수 처리 시 발아 및 초기생육기간 동안 담수내성이 약한 품종에서 SOD와 POX의 역할이 크고 ADH활성이 높게 유지되어 에너지를 공급할 수 있는 품종이 담수 시 초기생육에 유리할 것으로 사료된다.

• 한국약용작물학회지
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• 제14권1호
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• pp.45-48
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• 2006

순무는 초음파 병행 추출을 통하여 $60,\;100^{\circ}C$에서 모두 추출 수율을 증가시킬 수 있음을 확인하였다. 알코올 분해 효소인 ADH와 Aldehyde 분해 효소인 ALDH의 분해 활성 측정 실험을 통해 순무의 추출물들은 물 일반 추출물보다 초음파 병해 추출물들이 높은 활성을 나타내는 것을 확인하였고 특히 ADH의 활성율 보다 ALDH의 활성이 높게 나타나 알코을 분해 과정의 최종 목적지인 acetic acid와 이산화탄소로의 분해에 순무의 추출물들이 유의적인 활성을 나타내고 있음을 확인할 수 있었다. $60^{\circ}C$ 열수 추출물은 $100^{\circ}C$ 열수 추출물과 비교하여 관능평가 시 높은 점수를 얻었으며 이의 추출물들은 감압농축을 통해 이취의 제거가 이루어져 열수추출 시 유용한 방법으로 평가되었다.

• 대한한방내과학회지
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• 제24권1호
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• pp.84-93
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• 2003

Objectives : The aim of this study was to investigate effects of the Sungjuchunggan-tang(Xingjiuqinggan-tang), which has been used for alcoholic diseases in Oriental medicine, on alcoholic metabolism and alcoholic liver damage. Methods : We evaluated the activities of alcohol dehydrogenase(ADH)and aldehyde dehydrogenase(ALDH), and measured the levels of AST, ALT, glucose, triglyceride, HDL-cholesterol, LDL-cholesterol and phospholipid in serum of guinea pigs. Results and Conclusions : In this experiment, Sungjuchunggan-tang, Pharbitidis Semen and Puerariae Radix significantly suppressed the activity of ADH which is a precursor enzyme of acetaldehyde. Sungjuchunggan-tang and Pharbitidis Semen significantly suppressed the activity of ALDH, which is a catabolic enzyme, and increased its level. Due to alcohol consumption, level of ALT and AST were significantly reduced. These experimental results suggest that Sungjuchunggan-tang and Pharbitidis Semen inhibit the formation of acetaldehyde in the metabolism of alcohol, and affect the recovery of weakened liver functions caused by alcohol.

• 한국미생물·생명공학회지
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• 제25권4호
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• pp.386-390
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• 1997

The characteristics of alcohol dehydrogenase (ADH, EC 1.1.1.1, alcohol:NAD oxidoreductase) of thermotolerant alcohol-producing yeasts, Saccharomyces cerevisiae RA-74-2 and Kluyveromyces marxianus RA-912, were compared with that of mesophilic S. cerevisiae D, an industrial strain. Under anaerobic culture condition, both S. cerevisiae RA-74-2 and D had similar level of ADH activity at 30$\circ$C, and the activity of S. cerevisiae RA-74-2 at 37$\circ$C was the same level at 30$\circ$C. However, the level of ADH activity of S. cerevisiae D at 37$\circ$C decreased about 70% of that at 30$\circ$C. The level of enzyme activity of K. marxianus RA-912, which showed lower alcohol productivity than S. cerevisiae RA-74-2 and D, was about 43% of those strains at 30$\circ$C, and decreased somewhat at 37$\circ$C. The results showed a good correlation between the alcohol productivities and the level of ADH activities of these strains grown at 30$\circ$C and 37$\circ$C. And the higher heat stability of ADH of S. cerevisiae RA-74-2 than that of S. cerevisiae D seemed to reflect the ability of high temperature fermentation. Despite of its fermentation ability even at 45$\circ$C, however, the ADH of K. marxianus RA-912 showed lower heat stability than that of S. cerevisiae D. Both S. cerevisiae RA-74-2 and D showed similar patterns of two bands of ADH isozyme, and the low band of S. cerevisiae RA-74-2 moved slightly faster than that of S. cerevisiae D. The staining intensity of the bands of S. cerevisiae D at 37$\circ$C was weaker than those at 30$\circ$C. However, S. cerevisiae RA-74-2 showed no differences in total intensity of the bands of 30$\circ$C and 37$\circ$C. As the patterns of cellular proteins and ADH isozyme of K. marxianus RA-912 were different from S. cerevisiae RA-74-2 and D, K. marxianus might have its own characteristic ADH system.