• 대한수의학회지
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• 제15권1호
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• pp.19-22
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• 1975

The serum albumin phenotypes and the gene frquencies of 100 Korean native goats were examined by starch gel electrohporesis. The results obtained were as follows: 1. The albumin phenotypes were classified as Alb AA, Alb AB, and Alb BB and the frequencies of appearence were 10, 12 and 78%, respectively. 2. The genetic factors of serum albumin were observed as Alb A and Alb B and the rates of gene frequencies were 16 and 84%, respectively.

• 대한수의학회지
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• 제34권3호
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• pp.487-492
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• 1994

The study was conducted to investigate the phenotypes of blood proteins, hemoglobin and albumin, of 104 German shepherds in Dag-gu, Yae-chum Kim-hae and Kwang-ju area by the starch gel electrophoresis. The results obtained were summarized as follows: 1. The hemoglobin phenotypes were observed three(HbBB, HbBb and Hbbb). Fast migrating bands were same and slow migrating bands were divided by heavy(HbBB), light(HbBb) and non-stained(Hbbb). The frequencies of appearance in HbBB, HbBb and Hbbb were 17.31%, 51.92% and 30.77%, respectively. 2. The hemoglobin phenotypes were controlled by two allelic genes, $Hb^b$ and $Hb^B$. The gene frequencies were calculated at 0.567 in $Hb^B$ and 0.433 in $Hb^A$. 3. The albumin phenotypes were observed three(AlbFF, AlbSS and and AlbFS), which were divided by fast migrating band(AlbFF), slow migrating band(AlbSS) and mixed migrating band(AlbFS). The frequencies of appearance in AlbFF, AlbFS and AlbSS were 5.77%, 28.85% and 65.38%, respectively. 4. The albumin phenotypes were controlled by two alleic genes, $Alb^F$ and $Alb^S$. The gene frequencies were calculated at 0.202 in $Alb^F$ and 0.798 in $Alb^S$.

• 대한수의학회지
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• 제14권2호
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• pp.191-200
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• 1974

Hemoglobin, albumin and transferrin phenetypes observed in a population of 255 dogs of various breeds by agar gel electrophoresis for hemoglobin and albumin, and by starch gel electrophoresis, for transferrin. The results obtained were as follows: 1. The hemoglobin phenotypes were classified as HbAA, HbAB and HbBB and the frequencies of appearence were 91.77, 6.27 and 1.96%, respectively. Gene frequency studies of $Hb^A$ and $Hb^B$ showed the $Hb^A$ gene to have a frequency of 94.9% and $Hb^B$ gene to have a frequency of 5.1. The frequency of hemoglobin phenotypes of the Korean Jindo dog (ylelow) was HbAA 76.19%, HbAB 17.46% and HbBB 6.35% and HbBB was recognized only in the Korean Jindo dogs. 2. The albumin phenotypes were calssified as AlbAA, AlbAB and AlbBB and the frequencies of appearence were 28.71, 43.57 and 27.72% respectively. Gene frequency stduies of $Alb^A$ and $Alb^B$ showed the $Alb^A$ gene to hate a frequency of 50.50% and $Alb^B$ gene to have a frequency of 49.50. The frequency of albumin phenotypes of the Korean Jindo dog was 26.32% for AlbAA, 34.21% for AlbAB and 39.47% for AlbBB. The frequency of albumin phenotypes of the Korean Jindo dog was slightly different from others and gene frequency of $Alb^B$ was 56.58. 3. Transferrin studies resulted in findings of 10 different phenotypes and frequencies of transferrin phenotype were 30.08% for $TfA_1A_1$, 8.02 for $TfA_2A_2$, 2.11 for TfBB, 4.64 for $TfC_1C_1$, 1.69 for $TfA_1B$, 41.35 for $TfA_1C_1$, 5.49 for $TfA_2C_1$, 0.84 for $TfA_2C_2$ and 3.79 for $TfBC_1$. Gene frequency studies of $Tf^A$, $Tf^B$ and $Tf^C$ showed the gene to have a frequency of 63.08% for $Tf^A$, 4.85 for $Tf^B$ and 32.07 for $Tf^C$ 4. Transferrin studies revealed two different band patterns on starch gels with a strong and weaker stained one. The $TfC_2C_2$ and $TfA_2A_2$ which were found in the Korean Jindo dog only showed a weaker stained band and the gene frequency of $TfA_1A_1$ was highly recognized in this dog.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제50권4호
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• pp.206-215
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• 2024

Objectives: The current in vitro study aimed to assess the effects of ascorbic acid augmented albumin platelet-rich fibrin (AA Alb-PRF) on the wound healing activity of human gingival fibroblasts (HGFs) purported to be a regenerative biomaterial in surgical procedures. Materials and Methods: All assays were performed on three HGF groups, group I: complete media; group II: Alb-PRF, and group III: AA Alb-PRF. Alb-PRF was prepared following the protocol by Fujioka-Kobayashi et al. (2021). For preparation of AA Alb-PRF, 2,500 ㎍ AA was added to the blood pre-centrifugation. All groups were subjected to 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to estimate cell viability and proliferation, scratch assay for migration (0, 4, 12, and 24 hours) and transwell migration assay for chemotactic migration assessment (24 hours). Outcome variables were optical density (OD) for MTT assay, percentage of wound closure in scratch assay, and number of migrated cells in transwell migration assay. One-way ANOVA for MTT and transwell migration assays and two-way ANOVA for scratch assay with Bonferroni correction were performed with significance set at P<0.05. Results: Cell viability and proliferation (OD: 0.684±0.003 and proliferation: 28%) and wound closure (49.92%±1.62% at 4 hours and 61.39%±0.88% at 12 hours) were significantly higher in group III, while group II demonstrated the maximum number of HGFs migrating across the transwell membrane (9.25±2.49) with P<0.05. Conclusion: HGFs demonstrated a significant increase in viability and proliferation along with rapid wound closure in the presence AA Alb-PRF compared to Alb-PRF alone, indicating additional beneficial effects of AA. Thus, AA Alb-PRF potentiates the wound healing activity of HGFs and could be employed in oral, maxillofacial, and periodontal surgeries as a regenerative biomaterial.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제42권5호
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• pp.243-250
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• 2016

Objectives: Many studies have examined histopathological factors and various prognostic scores related to inflammation to predict outcomes. Here, we examined the prognostic value of the C-reactive protein/albumin (CRP/alb) ratio in oral squamous cell carcinoma (OSCC). Materials and Methods: This retrospective study included 40 patients with OSCC. Using univariate and multivariate analyses, we focused on the correlation of the CRP/alb ratio with clinicopathological characteristics and with overall survival. We then compared five inflammation-based prognostic scores, CRP/alb ratio, modified Glasgow Prognostic Score (mGPS), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and prognostic nutritional index (PNI), based on receiver operating characteristic (ROC) curves. Results: The optimal cut-off value for the CRP/alb ratio was 0.085. The group with a high CRP/alb ratio had a high TNM clinical stage (P=0.002) and larger primary tumors (P=0.029), with statistically significant differences in lymph node metastasis and distant metastasis. In addition, when the CRP/alb ratio was high, multivariate analysis showed a lower survival rate (P=0.002; hazard ratio=6.078), and the ROC curve showed more outstanding discriminatory ability regarding overall survival compared to other inflammation-based prognostic scores. Conclusion: The CRP/alb ratio can be an independent prognostic factor when predicting prognosis in OSCC and has good prognostic ability.

• 농업과학연구
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• 제25권1호
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• pp.68-78
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• 1998

한우집단의 혈액단백질 및 효소의 유전적 다형과 유전적 구성을 조사하기 위하여 축협중앙회에서 사육중인 한우집단에 대한 transferrin(Tf), post-transferrin-2(pTf-2), albumin(Alb), post-albumin(pAlb), ceruloplasmin(Cp), amylase-I(Am-I) 및 hemoglobin(Hb)의 유전적 변이체를 PAGE(polyacrylamide gel electrophoresis와 STAGE(starch gel electrophoresis) 방법으로 분석하였다. 혈청단백질의 유전적 변이체에 있어서 Tf유전자좌는 Tf A, $D_1$, $D_2$ 및 E 대립유전자의 지배를 받는 것으로 추정되었으며, 이들의 유전자빈도는 각각 0.249, 0.248, 0.260, 0.243이었다. pTf-2 유전자좌는 pTf-2 F와 S 대립유전자의 지배를 받는 것으로 확인되었으며, 이들의 유전자빈도는 각각 0.662 및 0.338이었고, pAlb 유전자좌는 pAlb F와 S 대립유전자의 지배를 받는 것으로 확인되었으며, 이들의 유전자빈도는 pAlb F와 S에서 각각 0.600 및 0.400이었다. 혈청효소의 유전적 변이체에 있어서 Cp유전자좌는 Cp F와 S, 그리고 Am-I유전자좌는 Am-IB와 C 대립유전자의 지배를 받는 것으로 확인되었으며, 이들의 유전자빈도는 Cp F와 S 에서 각각 0.319 및 0.681 이었고, Am-I B와 C에서 각각 0.318 및 0.682 이었다. Hb의 유전적 변이체에 있어서 Hb 유전자형 분포는 Hb AA, Ab 및 BB형에서 76.5, 21.2 및 2.3% 이었고, 유전자빈도는 Hb A 및 B에서 각각 0.871 및 0.129이었다. 산육형질에 대한 혈액단백질의 유전적 변이체의 효과에 있어서는 Tf $D_1D_1$, $D_2D_2$ 및 $D_2E$ 유전자형이 6개월 체중과 일당증체량에서 유의적으로 높았다.

• KSBB Journal
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• 제30권1호
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• pp.44-51
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• 2015

Chinese hamster ovary (CHO) cells are the most widely used mammalian host for the commercial production of recombinant proteins. However, they show relatively low yields of recombinant proteins in comparison with microbial cells. Various strategies have been tried to overcome this drawback. The acetyl moieties are attached to the N-terminus of histone by histone acetyltransferase (HAT) while histone deacetylase (HDAC) removes histone-bound acetyl groups. HDAC inhibitor (HDACi), such as sodium butyrate, sodium propionate and valproic acid, can enhance specific productivity of CHO cells. Human albumin-erythropoietin (Alb-EPO) is a novel 105 kDa protein comprising recombinant human EPO fused to human albumin. In this study, we examined the effects of HDACi on the production of Alb-EPO in CHO cells with various concentrations in the range of 0-1 mM. The results showed that sodium butyrate was found to be the best HDACi for enhancing productivity. It enhanced not only the production of Alb-EPO but also the apoptosis of recombinant CHO cells.

• KSBB Journal
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• 제28권2호
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• pp.86-91
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• 2013

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.

• 농업과학연구
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• 제22권1호
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• pp.69-81
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• 1995

본 연구는 축산기술연구소 대관령지원에서 사육하고 있는 Hereford, Angus 및 Charolais 육우품종에서 혈액을 채취하여 이들의 혈액내에 존재하는 transferrin(Tf), post-transferrin-2(pTf-2), albumin(Alb), post-albumin(pAlb), ceruloplasrmin(Cp), amylase-I(Am-I) 및 hemoglobin(Hb)의 혈액단백질 및 효소좌위의 유전적다형을 분석하여 이들 집단의 유전적 변이체를 조사하고자 PAGE(polyacrylamide gel electrophoresis)와 STAGE(starch gel electrophoresis)방법으로 분석하였던바 그 결과는 다음과 같다. 1. Tf좌위는 A, $D_1$, $D_2$ 및 E와 pTf-2좌위는 F와 S의 공우성 대립유전자의 지배를 받는 것으로 생각되며, 유전자형의 빈도에 있어서는 Hereford 종에서는 Tf $D_1D_2$와 pTf-2 SS 형이 각각 25 및 90%이었고, Angus종에서는 Tf $AD_1$와 pTf-2 FF형이 각각 25와 100%이었으며, Sharolais종에서는 Tf $D_1D_1$와 pTf-2 FS형이 각각 50%로 높은 빈도를 보였다. 유전자빈도에 있어서는 Hereford종에서 Tf E와 pTf-2 S 빈도가 각각 0.400과 0.900이었고, Sharolais종에서는 Tf $D_1$과 pTf-2 S가 각각 0.678 및 0.607로 높은 빈도를 보였다. 2. Alb좌위는 A, 그리고 pAlb좌위는 F와 S의 대립유전자의 지배를 받는것으로 사료되며, 유전자형 빈도에 있어서는 Hereford, Angus와 Sharolais종에서 각각 pAlb SS, FF 및 SS가 각각 70, 90 및 57.15%로 높은 빈도를 나타냈으며, pAlb의 유전자 빈도에 있어서는 Hereford와 Sharolais종에서 pAlb S가 각각 0.825와 0.750이었고, Angus종에서는 pAlb F가 0.900으로 높은 빈도를 보였다. 3. Cp 좌위는 F 및 S와 Am-I 좌위는 B와 C의 대립유전자의 지배를 받는 것으로 생각되며, 유전자형 빈도에 있어서는 Herford종에서는 Cp FF와 Am-I BB형의 빈도가 각각 100 및 85%이었고, Angus종에서는 Cp FF와 Am-I CC형이 각각 45 및 85%이였으며, Sharolais종에서는 Cp FF와 Am-I BC형이 각각 50 및 64.29%로 높은 빈도를 보였다. 유전자 빈도에서 있어서는 Herford, Angus와 Sharolais 종에서 Cp F가 각각 0.800, .875 및 0.680이었고 Hereford종에서 Am-I A가 0.800이었으며, Angus와 Sharolais종에서 Am-I C가 각각 0.875 및 0.680이었다. 4. Hb좌위 는 Hereford종과 Angus종에서는 Hb A, Sharol ais종에서는 Hb A와 B의 대립유선자가 검출 되었으며, Sharolais종에서 유전자형 빈도는 Hb AA 및 AB가 각각 57.14 및 42.88%이었고, 유전자빈도에 있아서는 Hb A와 B가 각각 0.785 및 0.215이었다.

• 농업과학연구
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• 제22권2호
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• pp.163-169
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• 1995

본 연구는 홀스타인종 유우의 혈청단백질 및 효소에 대한 유전적 구조를 분석하기 위하여, 혈청단백질인 post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb) 및 albumin(Alb)과 혈청효소인 ceruloplasmin(Cp)와 amylase-I(Am-I)을 polyacrylamide gel electrophoresis(PAGE)와 starch g디 electrophoresis(STAGE) 방법으로 유전적 변이체를 분석하였다. 혈청단백질인 pTf-2 좌위는 pTf-2 F와 S 유전자에 의해 지배되는 pTf-2 FF, FS 및 SS 유전자형이 확인되었으며, 이들의 분포는 각각 76.34%, 14.50% 및 9.10%이었고, 유전자빈도는 pTf-2 F와 S가 각각 0.836 및 0.164이었다. 한편 Tf 좌위는 Tf A, D1, D2 및 E 유전자가 검출되었으며, 유전자형은 Tf AA, AD1, AD2, AE, D1D1, D1D2, D2D2 및 D2E형이 확인되었고, 이들의 분포는 각각 0.11, 32.06, 19.08, 1.53, 10.69, 18.32, 9.92 및 2.29%이었으며, 유전자빈도는 Tf A, D1, D2 및 E에서 각각 0.324, 0.359, 0.298 및 0.019이었다. 또한 pAlb 좌위는 pAlb F와 S 유전자가 검출되었고, 유전자형은 pAlb FF, FS 및 SS형이 확인되었으며, 이들의 분포는 각각 32.06, 29.77 및 38.17%이었고, 유전자빈도는 pAlb F와 S가 각각 0.469 및 0.531이었다. Alb 유전자 빈도에 있어서는 Alb A와 B가 각각 0.996 및 0.004이었다. 그리고 혈청효소인 Cp 좌위는 Cp F와 S 유전자가 검출되었으며, 유전자형은 Cp FF, FS 및 SS 형이 확인되었고, 이들의 분포는 각각 46.57, 27.48 및 25.95%이었으며, 유전자빈도는 Cp F와 S가 각각 0.603 및 0.394이었다. Am-I 좌위는 Am-I B와 C 유전자가 검출되었으며, 유전자형은 Am-I BB, BC 및 CC 형이 확인되었고, 이들의 빈도비율은 각각 39.69, 21.73 및 38.93% 이었으며, 유전자 빈도는 Am-I B와 C가 각각 0.503 및 0.497이었다.