Genetic Variants of Serum Proteins and Enzymes in Holstein-Friesian Cattle

홀스타인종 유우의 혈청단백질 및 효소의 유전적 변이체

This study was carried out to examine the genetic constitution of serum proteins and enzymes in Holstein Friesian cattle population. The genetic variants of post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb), ceruloplasmin(Cp) and amylase-I(Am-I) were analyzed by using PAGE(polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis). In serum proteins, the pTf-2 locus were observed to be controlled by codominant alleles designated F and S, and the distribution of genotypes were 76.34, 14.50 and 9.10% for pTf-2 FF, FS and SS types, respectively. The gene frequencies of the pTf-2 F and S allele were 0.836 and 0.164. The Tf locus were found to be controlled by four alleles, Tf A, D1, D2 and E at a single locus, and the distribution of genotypes were 6.11, 32.06, 19.08, 1.53, 10.69, 18.32, 9.92 and 2.29% for Tf AA, AD1, AD2, AE, D1D1, D1D2, D2D2 and D2E type, respectively. The gene frequencies of the Tf A, D1, D2 and E wee 0.321, 0.359, 0.298 and 0.019. The pAlb locus were identified to be genetically controlled by two alleles, pAlb F and S allele, and the distribution of genotypes were 32.06, 29.77 and 38.17% for pAlb FF, FS and SS types, respectively. The gene frequencies of the pAlb F and S allele were 0.461 and 0.531. The Alb locus were observed to be controlled by Alb A and B allele, and the gene frequencies of these were 0.996 and 0.004. In serum enzymes, the Cp locus were found to be controlled by F and S allele, and the distribution of genotypes were 46.57, 27.48 and 25.95% for Cp FF, FS and SS types, respectively. The gene frequencies of F and S allele were 0.603 and 0.394. The Am-I locus were observed to be controlled by Am-I B and C allele, and the distribution of genotypes were 39.69, 21.73 and 38.93% for Am-I BB, BC and CC types, the gene frequencies of Am-I B and C were 0.503 and 0.497, respectively.

본 연구는 홀스타인종 유우의 혈청단백질 및 효소에 대한 유전적 구조를 분석하기 위하여, 혈청단백질인 post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb) 및 albumin(Alb)과 혈청효소인 ceruloplasmin(Cp)와 amylase-I(Am-I)을 polyacrylamide gel electrophoresis(PAGE)와 starch g디 electrophoresis(STAGE) 방법으로 유전적 변이체를 분석하였다. 혈청단백질인 pTf-2 좌위는 pTf-2 F와 S 유전자에 의해 지배되는 pTf-2 FF, FS 및 SS 유전자형이 확인되었으며, 이들의 분포는 각각 76.34%, 14.50% 및 9.10%이었고, 유전자빈도는 pTf-2 F와 S가 각각 0.836 및 0.164이었다. 한편 Tf 좌위는 Tf A, D1, D2 및 E 유전자가 검출되었으며, 유전자형은 Tf AA, AD1, AD2, AE, D1D1, D1D2, D2D2 및 D2E형이 확인되었고, 이들의 분포는 각각 0.11, 32.06, 19.08, 1.53, 10.69, 18.32, 9.92 및 2.29%이었으며, 유전자빈도는 Tf A, D1, D2 및 E에서 각각 0.324, 0.359, 0.298 및 0.019이었다. 또한 pAlb 좌위는 pAlb F와 S 유전자가 검출되었고, 유전자형은 pAlb FF, FS 및 SS형이 확인되었으며, 이들의 분포는 각각 32.06, 29.77 및 38.17%이었고, 유전자빈도는 pAlb F와 S가 각각 0.469 및 0.531이었다. Alb 유전자 빈도에 있어서는 Alb A와 B가 각각 0.996 및 0.004이었다. 그리고 혈청효소인 Cp 좌위는 Cp F와 S 유전자가 검출되었으며, 유전자형은 Cp FF, FS 및 SS 형이 확인되었고, 이들의 분포는 각각 46.57, 27.48 및 25.95%이었으며, 유전자빈도는 Cp F와 S가 각각 0.603 및 0.394이었다. Am-I 좌위는 Am-I B와 C 유전자가 검출되었으며, 유전자형은 Am-I BB, BC 및 CC 형이 확인되었고, 이들의 빈도비율은 각각 39.69, 21.73 및 38.93% 이었으며, 유전자 빈도는 Am-I B와 C가 각각 0.503 및 0.497이었다.