• Reproductive and Developmental Biology
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• 제38권4호
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• pp.147-158
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• 2014

Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a $15{\mu}M$ of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a $100{\mu}M$ concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.

• Toxicological Research
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• 제11권1호
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• pp.1-8
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• 1995

Fumonisins are a family of mycotoxins produced by the fungus Fusarium moniliforme which is a common contaminant in corn. Fumonisins are potent inhibitors of sphingosine and sphinganine N-acyltransferase (ceramide synthase), key enzymes in sphingolipid metabolism. The purpose of this study was to provide the evidence that the elevated levels of free sphingoid bases (primarily sphinganine) and depletion of complex sphingolipids were closely related to the inhibition of cell growth in LLC-$PK_1$ cells exposed to fumonisin $B_1$$(\leq 35 {\mu}M)$. Concentrations of fumonisin $B_1$ between 10 and $35 {\mu}M$ were known to inhibit cell growth without cytotoxicity in $LLC-PK_1$ cells (Yoo et al. Toxicol. Appl. Pharmacol. 114, 9-15, 1992). Cells exposed to 35$\mu M$ fumonisin B$_1$ for 48 and 72 hr developed a fibroblast-like (elongated and spindle-shaped) appearance and were less confluent than normal cells. At between 24 and 48 hr after exposure to fumonisin $B_1$ cells were beginning to show the inhibition of cell growth and at 72 hr the number of viable cells in fumonisin-treated cultures was about 50% of concurrent control cultures. During the 24 hr lag period preceding inhibition of cell growth, the free sphinganine levels in cells exposed to $35 {\mu}M$ fumonisin $B_1$ were highly elevated (approximately 230 fold higher than normal cells). The elevated levels of free sphinganine were $435\pm14$$pmoles/{10^6}$ cells at 48 hr and approximately TEX>$333\pm11$$pmoles/{10^6}$ cells in cells exposed to $35{\mu}M$ fumonisin$B_1$ at 72 hr, while the levels of free sphinganine in normal cells were less than 2$pmoles/{10^6}$ cells. Under the same condition, depletion of intracellular complex sphingolipids as a consequence of fumonisin inhibition of de novo sphingolipid biosynthesis and turnover pathway was appeared. Content of free sphingold bases in dividing cells was more elevated than in confluent cells at 24-48 hr after cells were exposed to $20{\mu}M$ fumonisin $B_1$. The dividing cells were showing the inhibition of cell growth at 48-72 hr and $20{\mu}M$ fumonisin $B_1$. The results of this study support the hypothesis that the inhibition of cell growth is very well related to the disruption of sphingolipid metabolism in $LLC-PK_1$ cells.

• 동의생리병리학회지
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• 제17권6호
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• pp.1453-1462
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• 2003

In order to study the effect of oral administration of Mawhangyounpye-tang against to asthma, astham was induced to allergy-sensitive Balb/c mouse with ovalbumin using method of Hatfield et al (1997). The changes of diameter lumen of upper portion of the trachea, lung weight, gross appearance of lung, histological profiles of lung and trachea, numbers of cellular compartments in the bronchoalveolar lavage fluid (BALF), numbers and morphology of the mast cells in the trachea, numbers of mucus-secretory cell in the broncus, morphology of the bronchus, ultramicroscopical appearance of surface of trachea and number of cilia and mucous-secretory cells by scanning electron microscope. Obtained results were as follows. 1. The diameters of trachea lumen were significantly decreased in asthma induced control groups and these decreasing were result from hypertrophy of mucous membrane. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 2. Lung weights and black spots, which were result from infiltration of inflammatory cells, were significantly increased in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 3. Hypertrophy of mucous membrane of trachea and bronchus and !bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposit in the lumen were observed in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 4. Cellular compartments including neutrophil and eosinophil were dramatically increased in the BALF of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 5. Mast cell degranulation and decreasing of the numbers of mast cells were detected in the trachea of asthma induced control groups. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 6. Shed, decreasing of cilia cell and increasing of mucous-secretory cells in the surface of the trachea of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. In conclusion, it Is considered that Mawhangyounpye-tang has somewhat favorable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased after oral administratin of Mawhangyounpye-tang in this study. In future, it is needed that the toxicological and dosagespecific study of Mawhangyounpye-tang to use against asthma with safe.

• 시큐리티연구
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• 제30호
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• pp.61-83
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• 2012

2011년 9월 알카에다 추종자가 단독으로 무인항공기에 폭약을 탑재하여 미 국방부와 의사당을 공격하려는 테러계획을 세웠다가 사전에 적발되었다. 또한 미국의 고성능 무인항공기 '센티널'의 기술이 2011년 말에 이란에게 유출되는 사고가 발생되었다. 테러세력이 무인항공기를 테러에 이용할 날이 머지않은 것이다. 본 연구의 목적은 무인항공기의 개념 이해와 지난 9월에 발생한 워싱턴 무인항공기 테러기도 사건 분석을 통해서 궁극적으로는 무인항공기를 이용한 테러에 대한 대응방안을 제언하여 제2의 9.11테러와 같은 대규모 인명피해를 사전에 예방하는 것이다. 세부적으로 본 연구에서는 무인항공기 테러의 첫 번째 대응방안으로 현행 항공법 등 관련 법률에서 무인항공기의 정의 및 분류, 처벌 조항에 대한 수정 및 신설을 제안하였다. 무인항공기의 급속한 발전 속도에 적절하게 부합하지 못하는 현행 관련 법령을 신설 및 개정하여 테러세력에게 경각심을 부여하고, 나아가 무인항공기 테러발생시 처벌 근거를 명확히 하여 일벌백계할 수 있는 제도적 장치를 마련해야 하는 것이다. 또한 현재 전 세계적으로 실시하고 있는 이원화된 공역운용 체계상으로는 신고 되지 않는 무인항공기의 공역 침범에 대한 사전인지 및 통제가 불가능하다. 이러한 취약성을 인식하고 본 연구에서 무인항공기 테러의 두 번째 대응방안으로 무인항공기와 유인항공기의 공역통합 운영을 제언하였다. 체계적인 공역통합 운용은 사전에 신고 되지 않거나 경로를 이탈한 유 무인 항공기를 적절하게 통제함으로써 항공기를 이용한 테러 기도를 미연에 방지할 수 있을 것이다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.472-472
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• 2004

Numerous studies on urban groundwater have been carried out in many other countries. Urban groundwater shows a unique hydrologic system because of complex urban characteristics such as road pavement, sewers and public water supply systems. These urban facilities may change the characteristics of groundwater recharge but contaminate its quality as well. There have been several researches on urban groundwater in Seoul. Seoul has been industrialized very rapidly so that the city has large population. The recent population in Seoul amounts to more than ten millions, corresponding to a very high density of about 17, 000 people/km$^2$. Therefore, many factors affect the groundwater quality and quantity in Seoul. Nowadays, groundwater in Seoul is being extracted for construction, industrial use, and drinking and so on. There are 15, 714 wells in Seoul and its annual usage is 41, 425, 977m$^3$(in 2001). Therefore, systematic studies are needed to properly manage and use the groundwater in Seoul. The purposes of this study in progress are to identify geochemical characteristics of groundwater in Seoul and to determine the extent of groundwater contamination and its relationship with urban characteristics. For this study, groundwater was sampled from more than 400 preexisting wells that were randomly selected throughout the Seoul area. For all samples, major cations together with Si, Al, Fe, Pb, Hg For 200 samples among them, TCE, PCE, BTEX were also analyzed by GC. Our study shows that groundwater types of Seoul are distributed broadly from Ca-HCO$_3$ type to Ca-Cl＋NO$_3$ type. The latter type indicates anthropogenic contamination. Among cations, Ca is generally high in most samples. In some samples, Na and K are dominant. The dominant anions change widely from HCO$_3$ to Cl＋NO$_3$. The anion composition is considered to effectively indicate the contribution of distinct anthropogenic sources. In addition, major ions are positively proportional to total dissolved solid (TDS) except K and NO$_3$. Thus, we consider that TDS may be used as an effective indicator of the extent of pollution. However, the increase of TDS may result from increased water-rock interaction. To determine the extent of groundwater contamination, it is needed to figure out the baseline water quality in Seoul. Furthermore, detailed geochemical studies are required to find out pollution sources and their corresponding hydrochemical parameters.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.1422-1426
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• 2009

본 연구에서는 댐 특성(기능, 규모)별로 하류하천에 미치는 유량변화 문제를를 체계적으로 분석하고자 수문변 화지표법(Indicators of Hydrologic Alteration, Richter et al., 1996)을 적용하여 한국수자원공사에서 관리하는 소양강댐 등 11개 다목적댐과 광동댐 등 12개 용수전용댐의 일단위 유입량과 방류량 자료를 이용, 연최소 최대유량 크기와 지속기간, 유량변화정도 등을 분석하여 댐운영이 하류하천에 미치는 유량변화를 비교분석 하였다. 광동댐 등 12개 용수전용댐의 월 유량변화의 산정 결과, 갈수기에 해당하는 10월부터 다음해의 6월까지 8개월간은 댐건설전 월평균 방유량은 $0.64m^3/sec\sim3.05m^3/sec$이었으나, 댐건설후 월평균 방류량은 $0.33m^3/sec\sim2.99m^3/sec$로 -69.7%$\sim$167.8%의 분포를 나타내고 있으나 12월과 1월을 제외하면 댐 건설 이후 오히려 6.8%$\sim$69.7% 가 감소하였다. 우기철인 7월부터 9월까지 3개월간은 댐건설전 월평균 방류량이 $6.82m^3/sec\sim7.67m^3/sec$이었으나, 댐건설후 월평균 방류량은 $4.12m^3/sec\sim4.35m^3/sec$로 51.0%$\sim$55.1%가 감소하여 저수지 공용량을 이용한 저류효과가 다소 있었음을 알 수 있다. 용수전용댐과 다목적댐의 분석 결과를 비교하여 보면, 용수전용댐은 1일 최소유량의 경우는 평균적으로 76.52%가 증가하였으나 3일부터 90일 최소유량의 54.19%$\sim$67.19%까지 감소하여 갈수기 하류유량 개선효과는 없으나, 다목적댐의 경우는 1일 최소 유량은 743.76%가 증가하였으며 90일 최소 유량도 83.62%가 증가하여 갈수기 하류유량 개선 효과가 큰 것으로 분석되었다. 용수전용댐의 1일 최대유량의 경우는 37.8%가 감소되며 90일 최대유량은 38.5$\sim$51.0%까지 감소하여 홍수기 댐의 저류효과가 점차 증가하였다. 다목적댐의 경우는 1일 최대 유량은 59.4%가 감소하였으며 감소량은 점차 감소하여 90일 최대강우량은 30.1%가 감소하여 홍수기 댐 저류효과로 하류홍수량을 상당히 조절함을 알 수 있다.

• 한국균학회지
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• 제20권1호
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• pp.1-10
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• 1992

1990년 8월에 5일간, 10월에 4일간, 1991년 7월에 5일간, 9월에 4일간, 도합 4차례에 걸쳐 채집한 100점의 표본과 1989년에 채집한 24점의 표본을 포함 하여 총 124 점의 구멍장이버섯류의 표본을 분류한 결과 이들 표본의 약 3/4이 최종적으로 동정되어 도합 1목, 4과, 22속, 33종으로 확인되었다. 그중 2속은 국내 미기록속 그리고 9종은 국내 미기록종으로 판명되어 우리나라의 균류목록에 새로이 추가되었다. 이들 미기록속 균류는 구멍장이버섯과의 황금구멍버섯속(屬)(신칭(新稱), Auriporia) 과 밀구멍버섯속(屬)(신칭(新稱), Ceriporiopsis)이며 , 미기록종 균류는 소나무비늘버섯과 의 가지진흙버섯(신칭(新稱), Phellinus laevigatus)와 구멍장이버섯과의 순황금구멍버섯(신칭(新稱), Auriporia aurulenta), 밀구멍버섯(신칭(新稱), Ceriporiopsis gilvescens), 큰밀구멍버섯(신칭(新稱), Ceriporiopsis subvermispora), 작은조개버섯(신칭(新稱), GloeoPhyllum trabeum), 큰살색구멍버섯(신칭(新稱), Junghuhnia separabilima), 흰손등버섯(신칭(新稱), Oligoporus stipticus), 구멍흰살버섯(신칭(新稱), Oxyporus similis) 및 검은발구멍장이버섯(신칭(新稱), Polyporus melanopus) 이였다. 또한 한국말 버섯이름 통일 위원회(1978)의 명명규칙에 따라 Antrodia를 구멍주름버섯속(屬)에서 주름구맹버섯(屬)으로 ,A η trodia al- bida 를흰그물송편버섯에서 흰주름구멍버섯으로, Antordia albida를 흰구멍송편버섯에서 흰주름구멍버섯으로, Antrodia sinuosa를 좀털구멍버섯에서 좀털주름구멍버섯으로 각각 개칭(改稱)하였다. 울릉도의 삼림은 칩엽수의 제한된 분포로 대부분의 구멍장이버섯들은 활엽수 특히 너도밤나무(Fagus crenata var. multinervis), 오리나무(Alnus), 그리고 마가목 (Sorbus)의 죽은 나무와 나무가지에 서식하고 있었으며 동정된 표본의 3/4 에 이르는 균류가 이들 활엽수에서 발견되었다. 침엽수 중에서는 소나무(Pinus)가 가장 좋은 기질을 제공하고 있으며 본 조사과정에서는 5종의 균류가 소나무에서 발견되었다. 또한 울릉도에서는 흰구름버섯(Coriolus hirsutus), 구름버섯(c. versicolor) 및 기계충버섯(Irpex lacteus)이 가장 흔한 우점종으로서 울릉도 삼림의 중요한 목재부후균류로 작용하고 있었다. 울릉도의 구멍장이버섯류는 전반적으로 육지의 균류상에 비하여 제한된 균류의 분포상을 보이고 있었으며, 섬의 환경에 잘 적응된 종류가 섬전역을 통하여 광범위하게 분포하는 반면에 미기록종을 포함한 일부 고유종들이 다수 출현하여 울릉도 균류상의 특정을 규정짓고 있었다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1062-1062
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• 2001

The concept of “precision agriculture” or “site-specific farming” is usually confined to the fields of soil science, crop science and agronomy. However, because plants grow in soil, animals eat plants, and humans eat animal products, it could be argued (perhaps with some poetic licence) that the fields of feed quality, animal nutrition and animal production should also be considered in this context. NIR spectroscopy has proved over the last 20 years that it can provide a firm foundation for quality measurement across all of these fields, and with the continuing developments in instrumentation, computer capacity and software, is now a major cog in the wheel of precision agriculture. There have been a few giant leaps and a lot of small steps in the impact of NIR on the animal world. These have not been confined to the amazing advances in hardware and software, although would not have occurred without them. Rapid testing of forages, grains and mixed feeds by NIR for nutritional value to livestock is now commonplace in commercial laboratories world-wide. This would never have been possible without the pioneering work done by the USDA NIR Forage Research Network in the 1980's, following the landmark paper of Norris et al. in 1976. The advent of calibration transfer between instruments, algorithms which utilize huge databases for calibration and prediction, and the ability to directly scan whole grains and fresh forages can also be considered as major steps, if not leaps. More adventurous NIR applications have emerged in animal nutrition, with emphasis on estimating the functional properties of feeds, such as in vivo digestibility, voluntary intake, protein degradability and in vitro assays to simulate starch digestion. The potential to monitor the diets of grazing animals by using faecal NIR spectra is also now being realized. NIR measurements on animal carcasses and even live animals have also been attempted, with varying degrees of success, The use of discriminant analysis in these fields is proving a useful tool. The latest giant leap is likely to be the advent of relatively low-cost, portable and ultra-fast diode array NIR instruments, which can be used “on-site” and also be fitted to forage or grain harvesters. The fodder and livestock industries are no longer satisfied with what we once thought was revolutionary: a 2-3 day laboratory turnaround for fred quality testing. This means that the instrument needs to be taken to the samples rather than vice versa. Considerable research is underway in this area, but the challenge of calibration transfer and maintenance of instrument networks of this type remains. The animal world is currently facing its biggest challenges ever; animal welfare, alleged effects of animal products on human health, environmental and economic issues are difficult enough, but the current calamities of BSE and foot and mouth disease are “the last straw” NIR will not of course solve all these problems, but is already proving useful in some of these areas and will continue to do so.

• 경영과정보연구
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• 제28권4호
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• pp.131-154
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• 2009

본 연구에서는 선행연구에 기초하여, 선행요인인 서번트 리더십, 매개변인인 상사신뢰와 결과요인인 조직성과에 어떠한 관계가 있는지에 대한 연구모델을 제시하고, 이들 변인간의 관련성을 실증분석을 통하여 분석하였다. 즉 서번트 리더십이 조직성과 변수인 직무만족, 조직몰입과 조직시민행동에 어떠한 영향을 미치며, 상사신뢰의 신뢰의 매개 역할을 분석하여 조직성과의 직접적, 간접적 영향요인을 파악하는데 있다. 본 연구의 연구결과를 요약하면 다음과 같다. 첫째, 서번트 리더십은 상사 신뢰와 정(+)의 관련을 갖는 것으로 나타났다. 둘째, 상사 신뢰의 역할과 관련하여 서번트 리더십과 결과변수와의 매개효과를 분석한 결과, 서번트 리더십은 결과변수에 직접적인 영향 뿐 아니라 상사 신뢰를 통해 간접적으로 영향을 미친다는 점이 확인되었다. 이것은 신뢰가 조직관련 연구 분야의 중요 개념이고, 조직 유효성을 설명하기 위한 중요 변수라는 Brower et al.(2000)의 주장과 일치한다고 할 수 있다. 다만 직무만족과 관련하여서는 상사 신뢰가 매개역할을 하지 않고 있는 것으로 나타났다. 셋째, 상사 신뢰가 결과변수에 미치는 영향에 대한 분석을 보면 직무만족, 조직몰입과 조직시민행동 정(+)의 유의한 영향을 보이고 있는 것으로 나타나고 있다. 특히, 상사 신뢰는 직무만족에 큰 영향을 미치는 것으로 나타나고 있다. 이러한 결과는 연구의 분석수준과 관련한 문제로 해석된다. 즉 직무만족은 개인차원의 조직성과 변수로 구성원 자신의 직접적인 행동과 태도와 관련된다. 상사 신뢰는 조직몰입과 조직시민행동과 같은 조직차원의 성과 변수에는 직무만족을 통해 간접적으로 영향을 받을 수 있음을 고려해 볼 수 있다.

• 가정∙방문간호학회지
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• 제9권2호
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• pp.103-113
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• 2002

Purpose: This study was conducted to explore the relationship among health belief. health locus of control and patients sick-role behavior compliance of diabetic mellitus patients visiting public health center. Method: The subjects of this study were 193 of the diabetic patients who were visiting 4 Public Health Center in B city. The instrument used for measuring health belief was Park's(1985). for health locus of control was Wallston. et al's(1978) and for sick-role behavior compliance was Park's(1984). The data were collected with structured questionnaires; total 58 items contained about health belief. health locus of control and sick-role behavior compliance from 1st to 31st July. 2001. The data was analyzed by the SPSS/PC programs using t-test. Pearson's correlation coefficient. ANOVA and Scheffe-test. Result: The average score of the health belief was $57.99\pm9.45$ health locus of control was $66.83\pm9.48$ and sick-role behavior compliance was $42.81\pm7.00$. Statistically significant factors influencing the health belief among social demographic characteristics were family number(F=3.818. p=0.024), monthly income(F=5.153, p=0.002), time of diagnosis(F=3.937. p=0.002) and difficult to control disease(F=5.803. p=0.000). The significant factors influencing the health locus of control were marital status(F=4.669. p=0.010). Also significant factors influencing the sick-role behavior compliance were monthly incomes(F=5.245, p=0.000). the time of diagnosis(F=4.424. p=0.001) and admission to hospital with diabetes(F=9.031. p=0.000). There was negative mild correlation comparatively between health belief and sickrole behavior compliance(r=-0.142, p<0.05) but no correlation in sensitiveness/severity, barrier, benefit(p<0.05). There was no correlation between internal. external. chance health locus of control and sick-role behavior compliance (P>0.05). Conclusion: There was a negative weak relationship between health locus of control and patient's sick role behavior compliance. Therefore further study to investigate the relating factor of the sick role behavior compliance among above of middle aged diabetes mellitus patients is necessary.