• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.243-248
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• 2011

The prevalence and distribution of hazardous microorganisms were investigated from the major perilla cultivation area at Milyang, Gyeongnam province, Korea. Aerobic plate count (APC) and coliform count of perilla leaves were 4.82 log CFU/g and 3.85 log CFU/g, respectively. E. coli, S. aureus and B. cereus were detected in 3.0% (4/114), 7.9% (9/114) and 46.5% (53/114) of examined perilla leaves. However, E. coli O157:H7, Salmonella spp, and L. monocytogenes were not detected. The distribution of hazardous microorganisms in perilla leaf cultivation environment were compared and the concentration of APC and coliform counts were more than 3.0 log CFU/(mL, g, $100cm^2$, hand) from most of the samples. S. aureus were detected from irrigation water, packing table, packing vinyl, hand, and clothes. Also, B. cereus was frequently detected from the examined samples. Especially, packing table and collection container were contaminated with maximum 5.5 log $CFU/100cm^2$ of B. cereus. Good Agricultural Practice (GAP) system should be introduced to farms to enhance the safety of perilla leaves.

• Journal of Food Hygiene and Safety
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• v.32 no.1
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• pp.20-26
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• 2017

Foodborne disease outbreaks associated with produces have been increasing in occurrence worldwide. This study investigated microbial contamination levels on thirteen kinds of agricultural products from farms stage to evaluate potential hazards associated with foodborne illness. A total of 1,820 samples were collected in major cultivating area from 2013 through 2015, and analyzed to enumerate aerobic bacterial counts, coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study for four kinds of microorganisms (Escherichia coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.01 to 7.18 log CFU/g, with the highest bacterial cell counts recorded for watermelon. Coliforms were detected in 651 samples (35.8%) with a minimum of 0.01 log CFU/g and a maximum of more than 5 log CFU/g. B. cereus was detected in 169 samples (9.3%) ranging from < 0.01 to 2.48 log CFU/g among total samples analyzed. S. aureus was detected in 14 samples (0.7%) with a minimum of 0.01 log CFU/g and a maximum of 1.69 log CFU/g. E. coli was detected in 101 samples (5.5%) among 1,820 samples. E. coli O157:H7, Salmonella spp. and L. monocytogenes were not detected in any of the samples. The microbial contamination levels of several agricultural products determined in this study may be used as the fundamental data for microbiological risk assessment (MRA).

• The Korean Journal of Pesticide Science
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• v.17 no.1
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• pp.50-59
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• 2013

This study was performed in order to assess the risk of korean orchard worker due to pesticide exposure. The amount of pesticide exposure was calculated based on the informations of 97 kind of items that are used the form of a spraying in Korean orchard. The risks of these pesticides were assessed to compare the exposure amount with AOEL (acceptable operator exposure level) which was released form developed countries. When the operator sprayed pesticides using speed sprayer, 74.2% of pesticide items showed the risk for operator if the operator have not worn the personal protective equipment (PPE), and 42.3% of pesticide items showed the risk for operator if the operator have worn the PPE. In case of using motor sprayer, 64.1% of pesticide items showed the risk for operator if the operator have not worn the PPE, and 19.4% of pesticide items showed the risk for operator if the operator have worn the PPE. This result was indicated that the risk of pesticide against the operator was very high in korean orchard operator.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.296-313
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• 2014

A QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) multiresidue method was developed for the simultaneous analysis of 124 pesticides in soil by LC-MS. The procedure involved liquid extraction of soil immersed with 0.2N $NH_4Cl$ by acetonitrile with 1% acetic acid, followed by anhydrous $MgSO_4$ and sodium acetate, and dispersive SPE cleanup with $MgSO_4$, primary secondary amine (PSA) and $C_{18}$. The extracts were analyzed with LC-MS/MS in ESI positive mode. Standard calibration curves were made by matrix matched standards and their correlation coefficients were higher than 0.99. Recovery studies for the validation were carried out using two type soils, loam and sandy loam, at four concentration levels (0.005, 0.01, 0.02, and 0.1 mg/kg). The recoveries of pesticides were in the range of 70-120% with < 20% RSD except 4 pesticides, Benfuracarb, Ethiofencarb, Pymetrozine, and Pyrethrin. This result indicated that the method using QuEChERS and LC-MS/MS could be applied for the simultaneous determination of pesticide residues in soils.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.228-235
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• 2014

The residue levels of flonicamid and its metabolites, 4-(trifluoromethyl)nicotinic acid (TFNA) and N-4-(trifluoromethyl)nicotinoyl glycine (TFNG) in sweer pepper were investigated to examine the residual characteristics of analytes for 87 days after pesticide application. The pesticide was applied once at recommended dosage and double dosage by foliar sprays and the samples of fruits and leaves of sweet pepper were collected for each treatment. The residues of flonicamid in all of fruits and leaves decreased gradually over time, while the residue levels of TFNG metabolite exhibited tendency that increased for long periods and thereafter decreased. Total flonicamid residual concentrations containing metabolites residues in fruit samples increased consistently until 30 days post-application and higher residue levels than residues at 1 day post-application were detected from 30 day to 87 day after treatment. The residue pattern observed in fruit could be explained by the movement of TFNG from leaves to fruits of plant. Such residual characteristic was similarly found in samples treated both recommended dosage and double dosage.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.423-432
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• 2022

The mass-rearing system for Neodryinus typhlocybae as a biological control agent of Metcalfa pruinosa was established. Depending on the density of host nymphs and plants, the average number of cocoons produced by the parasitoids was 5-8 and 70-150 cocoons per leaf and sapling of mulberry, respectively. There is a significant difference in cocoon length between females (6.10-6.46mm) and males (4.20-4.62mm). Sex determination of cocoons before emergence will be helpful for efficiently releasing this parasitoid in fields. The parasitic rate of N. typhlocybae at the semi-field condition was on average 13-17%. The release number of this parasitoid did not affect parasitism. Nevertheless, the population growth rate of M. pruinosa was reduced by increasing the release number of N. typhlocybae. The parasitoid offspring's sex and bivoltine were influenced by the host age. On young host nymphs, the bivoltine portion of parasitoid increased. When parasitized on 4^th or 5^th nymphs, the offspring's female ratio of N. typhlocybae increased. This result may be useful for potentially controlling mass rearing production of parasitoid.

• Journal of Food Hygiene and Safety
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• v.32 no.4
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• pp.321-328
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• 2017

Fusarium graminearum is a mycotoxigenic plant pathogen, which could remain in harvested barley grains and produces mycotoxins at preferable conditions during storage. To elucidate the factors affecting contamination of Fusarium and Fusariotoxin in hulled barley during storage, three hulled barley grain samples were collected from Jeolla province. Moisture content of each sample was adjusted to 14% and 20%, respectively, then stored in two warehouses where temperature was controlled differently: one controlled below $12^{\circ}C$, and the other with no control. While monitoring temperature and relative humidity of warehouses hourly, grain moisture content, Fusarium occurrence, and mycotoxin level was analyzed at 1, 3, 6, and 12 month after storage. The average monthly temperature and relative humidity ranged $3{\sim}29^{\circ}C$, and 58~70% in warehouse without temperature control, whereas $3{\sim}13^{\circ}C$ and 62~74% in warehouse controlled below $12^{\circ}C$. Grain moisture content of the samples decreased in both warehouses except 14% samples which increased in the warehouse with temperature control. Fusarium frequency of the contaminated grains decreased continuously in the warehouse without temperature control. But in the warehouse below $12^{\circ}C$, Fusarium decreasing rate was slower because of high grain moisture content. In most samples, nivalenol was detected more in the warehouse without temperature control after 12 month but there was little difference after 1, 3, and 6 month. Therefore, it will be efficient to store hulled barley in the warehouse controlled below $12^{\circ}C$ to reduce Fusarium contamination when the barley is not dried properly. In addition, when storage period exceeds 12 month, it is recommended to store hulled barley in a warehouse controlled below $12^{\circ}C$ to reduce nivalenol contamination.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.11-17
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• 2019

Strawberries are one of the main commodities in Korea and have been exported over 40 million dollar. Because the strawberry cultivation using hydroponics has increased, treatment of pesticide by drenching draw interest recently. However, detailed researches for drenching treatment of pesticide are limited, which results in difficulties in proper pesticide applications in agricultural fields. To activate use of drenching and improve safety in agricultural products, In this study, time-dependent residual characteristic of pesticides were compared with between different applications of pesticides in strawberries. The characteristics of azoxystrobin, prochloraz and thiamethoxam were investigated with drenching treatment at different applications: the time of treatment, concentration etc. at hydroponic cultivation for 40 days. Azoxystrobin and prochloraz were not detected at 14 day after application. Thiamethoxam was detected from 0.02 to 0.85 mg/kg. Crop uptake of pesticides was strongly affected with octanol-water partition coefficient and solubility in water. Residual amount in crops are highly dependent on the concentration of active ingredient of pesticides.

• Korean Journal of Environmental Agriculture
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• v.35 no.3
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• pp.191-201
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• 2016

BACKGROUND: The aim of this study was to investigate the antimicrobial effects of the ethanol extracts from various medicinal herbs against plant pathogens to understand the possible the crop protection agents.METHODS AND RESULTS: Among the tested medicinal herbs, Zizyphus jujuba ethanol extract had the potent antimicrobial activity against Phytophthora capsici, Erwinia carotovorum subsp. carotovora, Pseudomonas syringae pv. syringae and Ralstonia solanacearum. The major constituents of Z. jujuba were identified to eugenol(40.45%), dodecanoic acid(18.40%), β-caryophyllene (10.05%) and isoeugenol(9.85%) by GC/MS. Eugenol and isoeugenol had strong inhibitory activity on spore germination against P. capsici and growth against E. carotovorum subsp. carotovora, P. syringae pv. syringae and R. solanacearum.CONCLUSION: In this regard, eugenol and isoeugenol were found to be responsible for the antimicrobial activity of Z. jujuba ethanol extract against plant pathogens. In addition, Z. jujuba ethanol extract, eugenol and isoeugenol can be used the potent antimicrobial agents.

• Korean Journal of Environmental Agriculture
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• v.35 no.4
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• pp.241-246
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• 2016

BACKGROUND: Recently, the widespread distribution of pesticides in the hive has been of concern about pesticide exposure on honeybee (Apis mellifera L.) health. Larval toxicity was adapted to assess the synergistic and antagonistic interaction of cumulative mortality to the honeybee larvae of the four most common pesticides detected in pollen. METHODS AND RESULTS: Acetamiprid($3.0{\mu}l/L$), chlorothalonil ($803.0{\mu}l/L$), coumaphos ($128.0{\mu}l/L$), and tau-fluvalinate ($123.0{\mu}l/L$) were tested in combination; binary, ternary and four component mixture. Larvae were exposed to four pesticides mixed in diet at the average levels detected in pollen. As a result, synthetic toxicity was observed in the binary mixture of acetamiprid with coumaphos. The binary and ternary component mixtures of tested pesticides have mostly demonstrated additive effect in larval bees. The significant antagonistic effects were found in four parings of mixtures including chlorothalonil added to acetamiprid/tau-fluvalinate or acetamiprid/coumaphos/tau-fluvalinate, and tau-fluvalinate added to acetamiprid/chlorothalonil or acetamiprid/coumaphos/chlorothalonil. CONCLUSION: Interactions between combinations of four pesticides showed mostly additive or antagonistic effects in larval bees. Therefore, predicting the larval mortality of pesticides mixtures on the basis of the results of single pesticide may actually overestimate the risk. We suggest that pesticide mixture in pollen be evaluated by adding their toxicity together for complete data on interactions.