• Title/Summary/Keyword: Agriculture and Forestry

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Identification of QTLs controlling somatic embryogenesis using RI population of cultivar ${\times}$ weedy soybean

  • Choi, Pilson;Mano, Yoshiro;Ishikawa, Atsuko;Odashima, Masashi;Umezawa, Taishi;Fujimura, Tatsuhito;Takahata, Yoshihito;Komatsuda, Takao
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.23-27
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    • 2010
  • Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

Vacuum infiltration transformation of non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) with the pinII gene and bioassay for diamondback moth resistance

  • Zhang, Junjie;Liu, Fan;Yao, Lei;Luo, Chen;Zhao, Qing;Huang, Yubi
    • Plant Biotechnology Reports
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    • v.5 no.3
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    • pp.217-224
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    • 2011
  • Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is a popular vegetable in Asian countries. The diamondback moth (DBM), Plutella xylostella (L.), an insect with worldwide distribution, is a main pest of Brassicaceae crops and causes enormous crop losses. Transfer of the anti-insect gene into the plant genome by transgenic technology and subsequent breeding of insect-resistant varieties will be an effective approach to reducing the damage caused by this pest. We have produced transgenic non-heading Chinese cabbage plants expressing the potato proteinase inhibitor II gene (pinII) and tested the pest resistance of these transgenic plants. Non-heading Chinese cabbages grown for 45 days on which buds had formed were used as experimental materials for Agrobacterium-mediated vacuum infiltration transformation. Forty-one resistant plants were selected from 1166 g of seed harvested from the infiltrated plants based on the resistance of the young seedlings to the herbicide Basta. The transgenic traits were further confirmed by the Chlorophenol red test, PCR, and genomic Southern blotting. The results showed that the bar and pinII genes were co-integrated into the resistant plant genome. A bioassay of insect resistance in the second generation of individual lines of the transgenic plants showed that DBM larvae fed on transgenic leaves were severely stunted and had a higher mortality than those fed on the wild-type leaves.

Differential Proteomic Analysis of Chinese fir Clone Leaf Response to Salicylic Acid

  • Yang, Mei;Lin, Sizu;Cao, Guangqiu
    • Journal of Forest and Environmental Science
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    • v.26 no.2
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    • pp.83-94
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    • 2010
  • Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.

Characterization and Properties of Composites of Woodflour and Polylactic Acid

  • Febrianto, Fauzi;Yoshioka, Mariko;Nagai, Yuko;Syafii, Wasrin;Shiraishi, Nobuo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.5
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    • pp.67-78
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    • 2006
  • Modification of polylactic acid (PLA) and 10% maleic anhydride (MAH) with 15% dicumyl peroxide (DCP) based on MAH weight was conducted in the kneader at $160^{\circ}C$ and 30~70 rpm, for 15 min. The resulting MAH-modified PLA (PLA-MA) was then evaluated as a compatibilizer for PLA-wood flour (WF) composites. The FTIR and $^1H$-NMR analysis gave evidence of PLA-MA formation. After kneading and reacting with MAH and DCP, the number (Mn) and the weight average (Mw) molecular weights of PLA decreased as compared to the original PLA. The presence of WF in the composites decreased the tensile strength and several other physical properties. The higher the WF loading resulted in the greater the reduction of tensile strength. An addition of 10% PLA-MA as a compatibilizer to the composites improved the tensile strength and several other physical properties, increased the flow temperature, and decreased the melt viscosity. The improved composite revealed 1.42 times increased in tensile strength but not over PLA alone, and absorbed considerably less water compared to those of the composites free-compatibilizer.

Analysis of the Status of Agricultural Communities and Location Quotient (LQ) using Regional Survey Data in 2015 Census of Agriculture, Forestry, and Fisheries (2015 농림어업총조사 지역조사 자료를 이용한 농업공동체 현황과 지역별 입지계수(Location Quotient: LQ) 분석)

  • Lee, Jimin
    • Journal of Korean Society of Rural Planning
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    • v.26 no.2
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    • pp.83-93
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    • 2020
  • Rural areas have been faced with various problems such as aging and population decline, and in these circumstances, the activation of local communities is suggested as a solution. Agricultural producer organizations are a powerful means of maintaining rural society. The purpose of this study is to understand the characteristics of agricultural community activities by region by comparing the status of organization and activities and analyzing Location Quotient(LQ) based on the regional survey data in the 2015 census of agriculture, forestry, and fisheries. As the results of the analysis, it was found that 38.35% of villages participated in economic organizations and 32.22% of villages were having urban-rural exchange activities. The crop clubs were actively operated in the Gyeongsangbuk province, and other economic organizations were organized in Jeju, Haenam-gun, and Dangjin-si more than other regions. And the urban-rural exchange activities were found in Chungcheongnam province mainly. The results of detailed LQ analysis by organization and activity showed that specialized regions for each community or activity were different. These results could be used for regional planning or the establishment of strategies for community activation.

The Status and Prospects of Japanese Organic Foods System (일본의 유기식품 생산 및 관리제도 현황과 전망)

  • Jung, Man-Chul
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.177-197
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    • 2010
  • Japan launched its regulatory certification system for organic foods based on the amended JAS laws in June 2000, followed by the implementation of regulations on organic agricultural products and organic processed foods in January 2001 and with the implementation of the standards on organic animal husbandry and organic feed by the Ministry of Agriculture, Forestry & Fisheries in 2005, organic foods have been under integrated and systematic supervision. Certification of organic foods can be undertaken by registered accredited bodies (private certification bodies) or legal entities conforming to the regulations of the Ministry of Agriculture, Forestry & Fisheries. However, with the amendment of the JAS laws in March 2006, only legal entities conforming to the regulations of the ISO/IEC GUIDE 65 are eligible as certification bodies. Foreign organic products imported to Japan must be certified organic under JAS regulations and must be manufactured or produced by foreign manufacturers certified by local or foreign registered accredited organizations and distributed with the Organic JAS Mark affixed or through importers certified by local registered accredited organizations and distributed with the Organic JAS Mark affixed on the products. It can be implied from the Japanese case study that it necessary to reform the diverse agricultural products certification systems to set up an integrated certification system, and the restructuring of government organizations, reforms of environmentally-friendly agricultural products certification system and the integration of the organic food certification systems are needed in order to integrate the control of the standards and certification systems.

Factor Affecting Customers Satisfaction About Banking Services: Evidence from Agribank, Vietnam

  • NGUYEN, Dung Thi;NGUYEN, Ha Quang;PHUNG, Hanh My Tran;THAN, Hoang Viet
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.6
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    • pp.373-380
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    • 2022
  • Today, customer satisfaction is the most important issue for Vietnamese banks. There are numerous factors that influence bank customers' satisfaction. The study investigates the factors that influence customer satisfaction with deposit services at the Vietnam Bank for Agriculture and Rural Development (Agribank) in Bac Giang province, Vietnam, and analyses the impact of each component. The authors used 5-point Likert scales to assess whether elements were related to the factors determining consumer satisfaction with Agribank's deposit service. To perform this study, exploratory factor analysis (EFA) and multiple regression were used to find factors determining customer satisfaction with Agribank services. Customers that use deposit services at Agribank in Bac Giang province, Vietnam, provided samples of 300 survey questionnaires for this study. SPSS software is used to examine the data. Customers' satisfaction with Agribank's deposit service is influenced by six factors, including reliability, responsiveness, service capabilities, empathy, tangible means, and service price, according to the study. This finding also offers some recommendations for improving service quality in local Vietnamese banks to gain a competitive advantage.

Estimation of BOD Loading of Diffuse Pollution from Agricultural-Forestry Watersheds (농지-임야 유역의 비점원 발생 BOD 부하의 추정)

  • Kim, Geonha;Kwon, Sehyug
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.617-623
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    • 2005
  • Forestry and agricultural land uses constitute 85% of Korea and these land uses are typically mixed in many watersheds. Biological Oxygen Demand (BOD) concentration is a primary factor for managing water qualities of the water resources in Korea. BOD loadings from diffuse sources, however, not well monitored yet. This study aims to assess BOD loadings from diffuse sources and their affecting factors to conserve quality of water resources. Event Mean Concentration (EMC) of BOD was calculated based on the monitoring data of forty rainfall events at four agricultural-forestry watersheds. Exceedence cumulative probability of BOD EMCs were plotted to show agricultural activities in a watershed impacts on the magnitude of EMCs. Prediction equation for each rainfall event was proposed to estimate BOD EMCs: $EMC_{BOD}(mg/L)=EXP(0.413+0.0000001157{\times}$(discharged runoff volume in $m^3$)+0.018${\times}$(ratio of agricultural land use to total watershed area).