• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.321-334
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• 2017

Process surveillance within agricultural biogas plants (BGPs) was concurrently studied by high-throughput 16S rRNA gene amplicon sequencing and an optimized quantitative microscopic fingerprinting (QMF) technique. In contrast to 16S rRNA gene amplicons, digitalized microscopy is a rapid and cost-effective method that facilitates enumeration and morphological differentiation of the most significant groups of methanogens regarding their shape and characteristic autofluorescent factor 420. Moreover, the fluorescence signal mirrors cell vitality. In this study, four different BGPs were investigated. The results indicated stable process performance in the mesophilic BGPs and in the thermophilic reactor. Bacterial subcommunity characterization revealed significant differences between the four BGPs. Most remarkably, the genera Defluviitoga and Halocella dominated the thermophilic bacterial subcommunity, whereas members of another taxon, Syntrophaceticus, were found to be abundant in the mesophilic BGP. The domain Archaea was dominated by the genus Methanoculleus in all four BGPs, followed by Methanosaeta in BGP1 and BGP3. In contrast, Methanothermobacter members were highly abundant in the thermophilic BGP4. Furthermore, a high consistency between the sequencing approach and the QMF method was shown, especially for the thermophilic BGP. The differences elucidated that using this biphasic approach for mesophilic BGPs provided novel insights regarding disaggregated single cells of Methanosarcina and Methanosaeta species. Both dominated the archaeal subcommunity and replaced coccoid Methanoculleus members belonging to the same group of Methanomicrobiales that have been frequently observed in similar BGPs. This work demonstrates that combining QMF and 16S rRNA gene amplicon sequencing is a complementary strategy to describe archaeal community structures within biogas processes.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.457-463
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• 2002

이 연구는 x-선 투과영상을 이용하여 홍삼의 내공을 검출하기 위하여 수행되었다. 이를 위하여 x-선관에 조사되는 x-ray 양의 차이에 따른 위치별 밝기 값 차이를 보정하고 내공 검출에 적합한 영상처리 알고리즘을 개발하였다. 주요연구 결과를 요약하면 다음과 같다. 1) x-선관에 조사되는 x-ray양은 중심에서 가장자리로 갈수록 감소하며, 이것은 위치에 따른 gray 값의 분포가 달라지게 되는 원인이 되었다. 2) 홍삼을 2치화 하기 위해서는 동일한 x-선 조사강도에서 입력된 원 영상에서 빈 영상을 뺀 감산 영상을 만들어 줌으로써 가능하였다. 3) 조사강도 별 감산영상처리 결과 36kV/4.15mA와 39kV/4.15mA에서는 조사량이 많아서 홈삼 지근 부분의 영상이 손실되는 경우가 발생하였다. 4) 내공 검출을 위해서는 정상부분과 내공부분의 자기 값 차이를 크게 하는 전처리 영상을 만들 필요가 있었고, multiple 감산영상에서 뚜렷한 골짜기가 나타났으나 주근의 내공부분의 밝기값 보다 지근의 정상부분의 자기 값이 더 낮게 나타나 이를 보정하기 위하여 홍삼 위치에 따라 부분 보정된 알고리즘을 개발하였다. 5) x-선관의 놓인 위치에 따라서 내공판정시험 결과 중심에 있을 때에 비해 가장자리에 있을 때는 영상이 일부 손실되었으며, 중심 위치에서 서로 다른 굵기의 홍삼에 대해서는 모두 양호한 결과가 나타났다. 6) 완전한 홈삼으로 내공판정시험 결과 내공주위의 정상부분가지도 일부 내공으로 잘못 검출되었으나 이것을 재차 line profile에 의해 한 라인씩 문턱 값을 설정하여 내공만을 정확하게 판정하는 알고리즘을 개발하였다.양체의 접종작업은 모든 배양실이 인력에 의존하였으며, 배양체를 배지와 분리하여 불필요한 부분을 제거하고 배양작물에 따라 생육정도를 2~3등급으로 구분하여 배양용기의 배지 위에 치상하는 과정으로 수행되었으며, 작업능률은 호접란의 경우 배양병에 25본을 접종하는데 시간당 6병, 심비디움은 원형 플라스크에 25본을 접종하는데 시간당 10병 정도였다. 바. 식물체의 대량증식에 사용되는 플라스크, 배양병, PE용기 등 배양용기의 세척작업은 농원의 1개배양실에서 간이식 세척기, 이 외의 9개배양실은 모두 물에 담겨 두었다가 세제와 브러쉬 등을 사용하여 인력으로 세척하고 있어 생력화 기술개발이 요구되었다.도가 빠를수록 건조속도가 빨라졌으며, 건조에너지도 1,334kcal/kg.water로 비슷하게 소요되었다. 마. 시험구와 대비구의 건감률은 시험구에서 1.08~1.36w.b./h로 나타나 대비구보다 약 9.9~18.3%가 높게 나타났고, 건조에너지는 10.2~14.6%가 절감되었다. 발아율은 열풍온도가 낮을수록 높게 나타났고 시험구가 대비구보다 발아율이 낮게 나타났으며, 동할률 증가량도 원적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성

• Korean Journal of Remote Sensing
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• v.30 no.5
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• pp.571-586
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• 2014

Moderate Resolution Imaging Spectroradiometer (MODIS) provides air temperature (Tair) and dew point temperature (Tdew) profiles at a spatial resolution of 5 km. New Collection 6 (C006) MODIS07_L2 atmospheric profile product has been produced since 2012. The Collection 6 algorithm has several modifications from the previous Collection 5 (C005) algorithm. This study evaluated reliabilities of two alternative datasets of surface-level Tair and Tdew derived from C005 and C006 Aqua MODIS07_L2 (MYD07_L2) products using ground measured temperatures from 77 National Weather Stations (NWS). Saturated and actual vapor pressures were calculated using MYD07_L2 Tair and Tdew. The C006 Tair showed lower mean error (ME, -0.76 K) and root mean square error (RMSE, 3.34 K) than the C005 Tair (ME = -1.89 K, RMSE = 4.06 K). In contrasts, ME and RMSE of C006 Tdew were higher than those (ME = -0.39 K, RMSE = 5.65 K) of C005 product. Application of ambient lapse rate for Tair showed appreciable improvements of estimation accuracy for both of C005 and C006, though this modification slightly increased errors in C006 Tdew. The C006 products provided better estimation of vapor pressure datasets than the C005-derived vapor pressure. Our results indicate that, except for Tdew, C006 MYD07_L2 product showed better reliability for the region of South Korea than the C005 products.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.332-342
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• 2022

Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green ('Man-Choo' and 'Mat-Jjang') and red kale ('Red-Curled') cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the 'Man-Choo' cultivar, and four glucosinolates in the 'Mat-Jjang' and 'Red-Curled' cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the 'Red-Curled' cultivar, which followed by 'Man-Choo' and 'Mat-Jjang'. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.

• Animal Bioscience
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• v.36 no.3
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• pp.404-416
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• 2023

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

• Animal Bioscience
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• v.37 no.5
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• pp.896-907
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• 2024

Objective: The potential of aqueous extract of Christ's thorn jujube (Ziziphus spina-christi) leaves (SLAE) to reduce the negative impacts of heat stress on production performance and physiological traits was investigated in dual-purpose layers under subtropical farming. Methods: A total of 200, 25-week-old laying hens (Inshas strain) were randomly assigned to four dietary treatments including SLAE at 0, 2.5, 5.0, and 7.5 mL/kg, respectively. The average temperature-humidity index value was 26.69 during the experimental period. The SLAE contained saponin (0.045%), total flavonoid content of 17.9 mg of quercetin equivalent/100 g and overall antioxidant capacity concentration of 17.9 mg of ascorbic acid equivalent/100 g. Results: The maximum final body weight (BW), BW gain, egg weight, number, and mass occurred at the level of SLAE7.5 inclusion. The egg quality was significantly higher in SLAE groups than in control, and overall, SLAE7.5 had the most favorable influence at 28 and 32 weeks. Liver and kidney function, as well as lipids profile, improved significantly by SLAE inclusion; the lowest concentrations of these parameters were in SLAE7.5 hens. Treatment with SLAE7.5 increased total antioxidant capacity and endogenous antioxidant enzymes compared to control, whereas no effect on superoxide dismutase was noticed. Conclusion: The addition of SLAE at 7.5 mL/kg diet improved egg laying performance and quality, metabolic profiles, and antioxidant status during hyperthermia conditions.

• Journal of Biosystems Engineering
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• v.37 no.1
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• pp.44-50
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• 2012

Purpose: The characteristics of in-transit vibration stress and possible damage to packaged apples were examined. Methods: A simulated transportation environment with a specific power density profile was used for vibration impact experiments to determine the resulting decrease in packaged apple quality. Apples with or without vibration stress were stored at low temperature ($5{\pm}0.8^{\circ}C$, 75-85% relative humidity) for 30 days. Statistically significant differences (p ${\leq}$ 0.05) were found between apples with and without vibration stress for concentration of oxygen ($O_2$; 11.2% and 14.1%, respectively; initially $29{\pm}0.4%$), carbon dioxide ($CO_2$; 26.4% and 21.8%; initially $1{\pm}0.2%$), and ethylene (79.4 ${\mu}LL^{-1}$ and 55.6 ${\mu}LL^{-1}$; initially $14.1{\pm}0.6{\mu}LL^{-1}$) in the headspace of a gas-collecting container after 30 days of storage. Results: Significant differences were also measured for apples with and without vibration stress with respect to soluble solid content (15.4% and 14.9%, respectively; initially $12.9{\pm}0.8%$ and $13.1{\pm}1.1%$), weight loss (10.1% and 8.2%), and firmness (139.7 kPa and 163.3 kPa; initially $213.8{\pm}6.2$ kPa and $209.1{\pm}7.9$ kPa) after 30 days of storage. Conclusions: The vibration stress clearly accelerated the degradation of apple quality during storage, resulting in increased weight loss, soluble solid content, and headspace $CO_2$ and ethylene production, and decreased firmness and headspace $O_2$.

• Food Science of Animal Resources
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• v.38 no.5
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• pp.927-935
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• 2018

Various amounts of buckwheat powder (0%, 1%, 2%, and 3%) were added to emulsion-type pork sausages. The effects of buckwheat powder on the physicochemical characteristics of the emulsion type sausages, including proximate composition, cooking yield, viscosity, pH, instrumental color, texture profile analysis (TPA), and sensory evaluation, were determined. Increased levels of added buckwheat powder led to higher moisture (p<0.05), ash content (p<0.05 or >0.05), and cooking yield (p<0.05). However, the protein and fat contents of the sausage samples were lower (p<0.05) with higher levels of added buckwheat powder. The instrumental color and pH values were affected by the addition of buckwheat powder, which has a pH of 6.1 and associated color coordinates of $L^*=75.0$, $a^*=1.3$, and $b^*=8.7$. Meat batter with 3% buckwheat powder was shown the highest (p<0.05) viscosity indicating great formation of emulsion. There were no significant differences in the TPA among the treated samples. The viscosity value increased with increasing cooking yield in the batter ($r^2=0.7283$) i.e. the correlation coefficient between the two measurements was very high and positive. For sensory traits except for tenderness, the highest scores (p<0.05) was detected on sausages with 3% added buckwheat powder by a panel. These results suggest that the addition of 3% buckwheat powder to emulsion-type sausages produces a more beneficial meat product, with a lower fat content, greater formation of emulsion and no adverse effects on sensory properties.

• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.115-123
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• 2011

To investigate the effects of pipe materials on biofilm accumulation and water quality, an annular reactor with the sample coupons of four pipe materials (steel, copper, stainless steel, and polyvinyl chloride) was operated under hydraulic conditions similar to a real plumbing system for 15 months. The bacterial concentrations were substantially increased in the steel and copper reactors with progression of corrosion, whereas those in stainless steel (STS) and polyvinyl chloride (PVC) reactors were affected mainly by water temperature. The heterotrophic plate count (HPC) of biofilms was about 100 times higher on steel pipe than other pipes throughout the experiment, with the STS pipe showing the lowest bacterial number at the end of the operation. Analysis of the 16S rDNA sequences of 176 cultivated isolates revealed that 66.5% was Proteobacteria and the others included unclassified bacteria, Actinobacteria, and Bacilli. Regardless of the pipe materials, Sphingomonas was the predominant species in all biofilms. PCR-DGGE analysis showed that steel pipe exhibited the highest bacterial diversity among the metallic pipes, and the DGGE profile of biofilm on PVC showed three additional bands not detected from the profiles of the metallic materials. Environmental scanning electron microscopy showed that corrosion level and biofilm accumulation were the least in the STS coupon. These results suggest that the STS pipe is the best material for plumbing systems in terms of the microbiological aspects of water quality.

• Journal of Biosystems Engineering
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• v.16 no.2
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• pp.133-141
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• 1991

This study was undertaken to investigate the structural and configurational characteristics of the tailings return-unit in the commercially available head-feed combines and to study the aero-dynamical behavior of the tailings in the units. The mathematical model of the motion of tailings in the thrower casing was developed and the simulated trajectories for different type of units was analyzed to compare with the measured ones. The air-stream velocity profile in various locations along the tailings returning duct was measured to find the effect of configurational characteristics and blade tip speed. The results of the study are summerized as follows. 1. The ejecting angle, which is the angle between the direction of the particle velocity ejecting from the blade and the horizontal axis, was found to be about $66^{\circ}$ in both the simulation and experiment. The angle was much greater than the setting angle of actual duct of the combines studied, which were $48{\sim}56^{\circ}$. By comparison of these results, it was suggested to change duct setting angle so as to reduce the frictional force, between the duct wall and tailings, by reducing the difference between the ejecting and setting angles. 2. The velocity of the air stream in the duct was in general higher in the upper bound of the duct compared to the lower and decreased as the stream went toward the end of duct. The comparison of the tailings units among the combines studied showed a superior performance with the tapered duct having small diameter in the outlet and with greater number of thrower blade.