• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.89-98
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• 2016

Endophytes play an important role in the growth and development of the host. However, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. We studied the bacteria associated with fruiting bodies of Tricholoma matsutake picked from seven main T. matsutake-producing areas in Sichuan, China, by barcoded pyrosequencing. About 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and archaea, and 361-797 operational taxonomic units were observed at a 97% similarity level. The bacterial community was always both more abundant and more diverse than the archaeal community. UniFrac analysis showed there were some difference of bacterial communities among the samples sites. Three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, were dominant in all samples. Correlation analysis showed there was a significant correlation between some soil properties and bacterial community associated with T. matsutake. This study demonstrated that the bacteria associated with T. matsutake fruiting bodies were diversified. Among these bacteria, we may find some strains that can promote the growth of T. matsutake.

• 한국토양비료학회지
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• 제46권3호
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• pp.182-186
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• 2013

In this study, we examined the chemical properties and microbial community characteristics in 25 controlled horticultural soils (CHS) sampled from Gyeongnam Province by fatty acid methyl ester (FAME) method. The electrical conductivity of watermelon CHS was significantly (p < 0.05) higher than those of red pepper CHS, pumpkin CHS, and strawberry CHS. The amounts of total FAMEs, total bacteria, gram-negative bacteria, gram-positive bacteria, and fungi were significantly (p < 0.05) higher in red pepper CHS than those in strawberry CHS and pumpkin CHS. In addition, higher (p < 0.05) ratios of cy19:0 to $18:1{\omega}7c$ were detected in tomato CHS than those in watermelon CHS, pumpkin CHS, and red pepper CHS. This implied that microbial communities of tomato CHS were stressed more than other species of cultivation soils. Actinomycetes community in red pepper CHS was significantly (p < 0.05) higher than those in tomato CHS, strawberry CHS, and watermelon CHS. Differences in soil microbial community composition were highly associated with cultivated crop species which might result from the management inputs such as fertilizer, herbicide, and irrigation.

• 한국농공학회논문집
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• 제63권3호
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• pp.97-105
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• 2021

Most of the rural development projects for the welfare of residents are mainly new construction and remodeling projects for community buildings such as village halls and senior citizens. However, in the case of the construction industry, it has been studied that 23% of the total carbon dioxide emissions generated in Korea are generated in the building-related sector. (GGIC, 2015) In order to reduce the emission of environmental pollutants resulting from construction of rural community buildings, there is a need to establish a system for rural buildings by predicting the environmental impact. As a result of this study, the emissions of air pollutants from buildings in rural communities were analyzed by dividing into seven stages: material production, construction, operation, maintenance, demolition, recycling, and transportation activities related to disposal. As a result, 12 kg of carbon dioxide (CO), 0.06 kg of carbon monoxide (CO), 0.02 kg of methane (CH), 0.04 kg of nitrogen oxides (NO), 0.02 kg of sulfurous acid gas (SO), and non-methane volatile organics per 1m of buildings in rural communities It was analyzed that 0.02 kg of compound (NMVOC) and 0.00011 kg of nitrous oxide (NO) were released. This study proved that environmentally friendly design is possible with a quantitative methodology for the comparison of operating energy and air pollutant emissions through the design specification change based on the statement of the rural community building. It is considered that it can function as basic data for further research by collecting major structural changes and materials of rural community buildings.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2002년도 학술발표회 발표논문집
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• pp.113-116
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• 2002

The rural population has been continuously decreased since the standards of living in rural communities is relatively lower than in urban. In other words, rural area has become less attractive for settlement. The concept of 'rural amenity' was introduced, to increase interests in the development of rural area. But, there has not been proper theories and methods to evaluate the amenity. In this study, rural amenity was classified and the elements of rural amenity was evaluated using statistical methods. The results of this study could be useful to assess the effects of the polices on improving rural amenities.

• 농촌계획
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• 제4권1호
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• pp.40-55
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• 1998

The objectives of this study are to 1)research the importance of direct trade of agricultural products between farmers and consummers, 2)invest the trade process of agricultural products, 3)survey the patterns of direct trade of agricultural products, 4)find out the situations of direct trade of agricultural products, 5)suggest the directions to improve the direct trade of agricultural products. The data were collected from Taegu city, Taechun city and Kyungsang poop do for this study. The major findings of this study to improve the direct trade of agricultural products between urban and rural are as follows; First, it is very important that producers and consummers have common sense to the direct trade between urban and rural. Second, local government related the trade of agricultural products should positively take parts in the direct trade of agricltural products between producers and consummers in administration and finance. Thirth, non-profit organizations above other organizations in urban and rural must lead the direct trade of agricultural products between urban and rural. Fourth, the markets for direct trade of agricultural products are to be opened according to basing on consummers'needs to agricultural products. Fifth, the engaged farmings between producers and consummers are to be more positive by significant relationship( promised brothers and sisters) between rural and urban. Sixth, people who moved to urban from rural should be finked out in each community(in rural), they can buy the agricultural products in the community that they were born. Seventh, The communities in rural must produce the specific agricultural products in each community.

• 한국환경농학회지
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• 제39권1호
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• pp.65-74
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• 2020

BACKGROUND: Soil carbon sequestration has been investigated for a long time because of its potential to mitigate the greenhouse effect. No- or reduced tillage, crop rotations, or cover crops have been investigated and practiced to sequester carbon in soils but the roles of soil biota, particularly microorganisms, have been mostly ignored although they affect the amount and stability of soil organic matters. METHODS AND RESULTS: In this study we analyzed the organic matter and microbial community in organically cultivated corn field soils where no-tillage (NT) or conventional tillage (CT) had been practiced for about three years. The amounts of organic matter and recalcitrant carbon pool were 18.3 g/kg dry soil and 4.1 g C/kg dry soil, respectively in NT soils, while they were 12.4 and 2.5, respectively in CT soils. The amounts of RNA and DNA, and the copy numbers of bacterial 16S rRNA genes and fungal ITS sequences were higher in NT soils than in CT soils. No-tillage treatment increased the diversities of soil bacterial and fungal communities and clearly shifted the bacterial and fungal community structures. In NT soils the relative abundances of bacterial phyla known as copiotrophs, Betaproteobacteria and Bacteroidetes, increased while those known as oligotrophs, Acidobacteria and Verrucomicrobia, decreased compared to CT soils. The relative abundance of a fungal phylum, Glomeromycota, whose members are known as arbuscular mycorrhizal fungi, was about two time higher in NT soils than in CT soils, suggesting that the higher amount of organic matter in NT soils is related to its abundance. CONCLUSION: This study shows that no-tillage treatment greatly affects soil microbial abundance and community structure, which may affect the amount and stability of soil organic matter.

• 농촌지도와개발
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• 제12권1호
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• pp.17-28
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• 2005

This study investigated structural changes of the Korea's agriculture and proposed the new direction of rural and agricultural policy in Korea after economic opening. The major results may be summarized as follows: Firstly, the study identified structural changes of the country's peasantry under the economic opening system. Korean agriculture has been deteriorated in population structure, arable land size, and income composition. Secondly, according to above analysis on these changes it suggested that the traditional rural communities were exposed to collapse and this unwanted disaster could result in serious adversities to the national economy. Thirdly, this study proposed preservation policies for small scale farming households and also administrative preparations of multi-dimensional approaches leading to successful rural and agricultural planning.

• Animal Bioscience
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• 제34권11호
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• pp.1784-1793
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• 2021

Objective: An experiment was conducted to evaluate the effects of bamboo leaf extract (BLE) on the production performance, rumen fermentation parameters, and rumen bacterial communities of heat-stressed dairy cows. Methods: The experiment comprised a 14-day adaptation period and a 21-day experimental period and was conducted in a high-temperature and humidity environment (daily mean ambient temperature = 33.5℃±1.3℃; daily mean relative humidity = 64.9%±0.8%, daily mean temperature-humidity index = 86.2±0.4). Twelve Holstein dairy cows were randomly allocated into two groups. A total mixed ration supplemented with BLE at 0 (CON) and 1.3 g/kg dry matter (DM) were fed, respectively. Feed intake and milk yield were recorded daily. Milk samples were collected on 1, 11, and 21 d of the experimental period to analyze milk performance. Rumen fluid samples were collected on 21 d of the experimental period to analyze rumen fermentation parameters and rumen bacterial communities. Results: Compared with the control group, supplementation of BLE increased milk yield (p<0.01), milk fat yield (p = 0.04), 4% fat-corrected milk (p<0.01) and milk fat content (p<0.01); reduced somatic cell count (p<0.01). No differences in DM intake and milk protein or lactose content were observed between two groups. Supplementation of BLE also increased the rumen total volatile fatty acid (p<0.01), acetate (p<0.01), butyrate (p<0.01), and valerate (p = 0.05) concentrations. However, no significant effects were observed on rumen pH, ammonia nitrogen, propionate, acetate/propionate ratio, isobutyrate, or isovalerate. Furthermore, BLE increased the rumen bacterial abundance and the diversity of the rumen bacterial community. The BLE reduced the Firmicutes/Bacteroidetes abundance ratio and increased the abundances of Butyrivibrio_2 (p<0.01) and Ruminococcus_2 (p<0.01). Conclusion: The BLE supplementation at 1.3 g/kg DM could improve production performance and rumen fermentation in dairy cows during heat stress.

• The Plant Pathology Journal
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• 제38권6호
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• pp.692-699
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• 2022

Bacterial wilt caused by Ralstonia solanacearum is considered one of the most harmful diseases of pepper plants. Recently, research on plant disease control through the rhizosphere microbiome has been actively conducted. In this study, the relationship with disease occurrence between the neighboring plant confirmed by analyzing the physicochemical properties of the rhizosphere soil and changes in the microbial community. The results confirmed that the microbial community changes significantly depending on the organic matters, P₂O₅, and clay in the soil. Despite significant differences in microbial communities according to soil composition, Actinobacteriota at the phylum level was higher in healthy plant rhizosphere (mean of relative abundance, D: 8.05 ± 1.13; H: 10.06 ± 1.59). These results suggest that Actinobacteriota may be associated with bacterial wilt disease. In this study, we present basic information for constructing of healthy soil in the future by presenting the major microbial groups that can suppress bacterial wilt.

• 환경생물
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• 제38권4호
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• pp.594-602
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• 2020

지형과 고도가 논둑에 서식하는 토양성거미의 군집과 다양성에 미치는 영향을 조사하였다. 지형의 영향평가를 위해 벼재배기에 산간지와 평야지에서 비교한 결과 거미의 군집구조가 달랐으며 발생밀도는 산간지보다 평야지에서 높았다. 늑대거미과가 가장 우점하였으며 이사고늑대거미(P. isago)의 발생밀도는 산간지보다 평야지에서 높았다. 거미군집의 발생은 국지(100 km²) 및 지역(1,000 km²) 범위에서 유사하였다. 고도의 영향과 관련하여 휴한기에 지형이 유사한 장수와 정읍 지역을 비교한 결과 발생밀도는 지역 간 차이가 없었으나, 긴마디늑대거미속(Pardosa spp.) 3종의 발생밀도는 정읍에서 높았다. 이러한 결과는 지형과 고도가 군집 수준에서 토양성 논거미의 다양성지수에 미치는 영향은 크지 않으나, 종 수준에서 논에 의존적인 늑대거미과의 우점종에 영향을 줄 수 있다는 것을 보여준다.