• Asian-Australasian Journal of Animal Sciences
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• 제33권1호
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• pp.61-68
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• 2020

Objective: The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods: A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results: The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion: In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus.

• The Plant Pathology Journal
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• 제34권4호
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• pp.286-296
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• 2018

Maintenance of a beneficial microbial community, especially in the rhizosphere, is indispensable for plant growth and agricultural sustainability. In this sense, plant growth-promoting rhizobacteria (PGPR) have been extensively studied for their role in plant growth promotion and disease resistance. However, the impact of introducing PGPR strains into rhizosphere microbial communities is still underexplored. We previously found that the Proteus vulgaris JBLS202 strain (JBLS202) promoted growth of Kimchi cabbage and altered the relative abundance of total bacteria and Pseudomonas spp. in the treated rhizosphere. To extend these findings, we used pyrosequencing to analyze the changes in bacterial communities in the rhizosphere of Kimchi cabbage after introduction of JBLS202. The alterations were also evaluated by taxon-specific realtime PCR (qPCR). The pyrosequencing data revealed an increase in total bacteria abundance, including specific groups such as Proteobacteria, Acidobacteria, and Actinobacteria, in the treated rhizosphere. Time-course qPCR analysis confirmed the increase in the abundance of Acidobacteria, Actinobacteria, Alphaproteobacteria, and Betaproteobacteria. Furthermore, genes involved in nitrogen cycling were upregulated by JBLS202 treatment indicating changes in ecological function of the rhizosphere soil. Overall, these results indicate that introduction of JBLS202 alters both the composition and function of the rhizosphere bacterial community, which can have direct and indirect effects on plant growth. Therefore, we propose that long-term changes in bacterial composition and community-level function need to be considered for practical use of PGPRs.

• 한국학교수학회논문집
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• 제18권1호
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• pp.123-148
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• 2015

정부의 사교육 경감 대책 및 수학교육 선진화 방안이 발표되고, 교육부에서는 '농산어촌 ICT 지원 사업'으로 농산어촌의 교육정보화 격차를 줄이는 사업을 진행하고 있다. 또한 한국교육방송(EBS)에서는 EBSmath 라는 수학 자기 주도 학습 사이트를 개설하고, 다양한 맞춤형 서비스를 제공하고 있다. '농산어촌 ICT 지원 사업'에서 지원된 스마트기기를 활용하여 EBSmath 사이트를 활용한 학습이 여러 가지의 교육정보화가 열악한 농촌학생들에게 스스로의 힘으로 지식을 습득하고 체계적으로 정리하여 새로운 지식을 산출하는 능력, 즉 수학 자기주도 학습에 어떠한 영향을 미치는지에 대하여 본고에서 연구하였다.

• Journal of Ecology and Environment
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• 제42권4호
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• pp.139-149
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• 2018

Background: Ethiopia is among the poorest countries where land degradation caused livelihood problem to its inhabitants. The livelihood of rural communities in Ethiopia is seriously threatened by land degradation. Land is the major natural resource that economic, social, infrastructure, and other human activities are undertaken on. Thus, land resources play an important role in shaping rural livelihoods, and lack of sustainable land management practices leads to land degradation. Thus, this study aimed to analyze interlink between land degradation and livelihood of rural communities in Chilga district, Northwest Ethiopia. It also addresses the factors which influence income diversification for livelihood of households in the study area. Result: The result depicts that the major causes of land degradation are both natural and anthropogenic. Land degradation and livelihood are negatively interlinked with each other. The livelihood of the majority of the population in the study area is dependent on subsistence agriculture both farming and animal husbandry with low diversification. The survey result showed that more than half (69%) of the sample households have farm size of less than 2 ha, nearly one third (31%) have 2.0-2.5 ha, and insignificant number of farmers have more than 2.5 ha. More than 80% of the respondents pointed out that land degradation has impacts both on crop yield and livestock production. Most of the explanatory variables such as gender, age, education level, farmland size, and family size have statistical significant influence (at P < .01 and P < .05 levels) for income diversification of households, while marital status on the other hand is not statistically significant though it has positive relation with income diversification in this study. Conclusions: Our results suggest awareness should be created in the community about the livelihood diversification mechanisms which enabled them to engage in different income-generating activities and comprehensive watershed management should be implemented.

• Journal of Ginseng Research
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• 제43권1호
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• pp.77-85
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• 2019

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1701-1708
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• 2021

Food safety is the most important global health issue due to foodborne pathogens after consumption of contaminated food. Foodborne bacteria such as Escherichia coli, Salmonella enterica, Staphylococcus aureus, Campylobacter spp., Bacillus cereus, Vibrio spp., Yersinia enterocolitica and Clostridium perfringens are leading causes of the majority of foodborne illnesses and deaths. These foodborne pathogens often come from the livestock feces, thus, we analyzed fecal microbial communities of three different livestock species to investigate the prevalence of foodborne pathogens in livestock feces using metagenomics analysis. Our data showed that alpha diversities of microbial communities were different according to livestock species. The microbial diversity of cattle feces was higher than that of chicken or pig feces. Moreover, microbial communities were significantly different among these three livestock species (cattle, chicken, and pig). At the genus level, Staphylococcus and Clostridium were found in all livestock feces, with chicken feces having higher relative abundances of Staphylococcus and Clostridium than cattle and pig feces. Genera Bacillus, Campylobacter, and Vibrio were detected in cattle feces. Chicken samples contained Bacillus, Listeria, and Salmonella with low relative abundance. Other genera such as Corynebacterium, Streptococcus, Neisseria, Helicobacter, Enterobacter, Klebsiella, and Pseudomonas known to be opportunistic pathogens were also detected in cattle, chicken, and pig feces. Results of this study might be useful for controlling the spread of foodborne pathogens in farm environments known to provide natural sources of these microorganisms.

• Journal of Forest and Environmental Science
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• 제39권3호
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• pp.167-179
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• 2023

The extent of change in the Land use/Land cover (LULC) of Okomu National Park (ONP) and fringe communities was evaluated. High resolution Landsat imagery was used to identify the major vegetation cover/land use systems and changes around the national park and fringe communities while field visits/ground truthing, involving the collection of coordinates of the locations was carried out to ascertain the various land cover/land use types identified on the images, and the extent of change over three-time series (2000, 2010 and 2020). The change detection was analyzed using area calculation, change detection by nature and normalized difference vegetation index (NDVI). The result of the classification and analysis of the LULC Change of ONP and fringe communities revealed an alarming rate of encroachment into the protected area. All the classification features analyzed had notable changes from 2000-2020. The forest, which was the dominant LULC feature in 2000, covering about 66.19% of the area reduced drastically to 36.12% in 2020. Agricultural land increased from 6.14% in 2000 to 34.06% in 2020 while vegetation (degraded land) increased from 27.18% in 2000 to 38.89% in 2020. The magnitude of the change in ONP and surroundings showed the forest lost -247.136 km² (50.01%) to other land cover classes with annual rate change of 10%, implying that 10% of forest land was lost annually in the area for 20 years. The NDVI classification values of 2020 indicate that the increase in medium (399.62 km² ) and secondary high (210.17 km² ) vegetation classes which drastically reduced the size of the high (38.07 km² ) vegetation class. Consequent disappearance of the high forests of Okomu is inevitable if this trend of exploitation is not checked. It is pertinent to explore other forest management strategies involving community participation.

• Journal of Forest and Environmental Science
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• 제33권1호
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• pp.8-21
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• 2017

The objective of this study is to identify the individual characteristics and the elements of social capital hence a suitable design of social capital reinforcement can be proposed to promote a sustainable community-based mangrove management. The research conducted in three villages that were selected based on mangrove ecological differences in Lampung Province, Indonesia. Qualitative data was collected through field observation and in-depth interviews with key informants. The results showed that the population in the three villages dominated on productive ages, worked as farmers, and less educated (only a half of the population had fulfilled the basic education standard of Indonesia). The study results also indicated that the social capital in the communities showed minimum condition of interpersonal attachment and cooperation. This condition was derived from the attitude of the people who only took personal benefit without concerning to others' welfare. Many programs conducted by government to ensure the conservation of mangroves were project-oriented with minor participation of community. The minor participation might also contributed to the minimum of social capital in the community. To improve social capital, the communities should strengthen mutual trust based on mutual benefit to increase members' participation in mangrove activity.

• Mycobiology
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• 제45권3호
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• pp.150-159
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• 2017

This study analyzed the distribution of endophytic fungi in 3 coastal environments with different climatic, geographical, and geological characteristics: the volcanic islands of Dokdo, the East Sea, and the West Sea of Korea. The isolated fungal endophytes were characterized and analyzed with respect to the characteristics of their host environments. For this purpose, we selected common native coastal halophyte communities from three regions. Molecular identification of the fungal endophytes showed clear differences among the sampling sites and halophyte host species. Isolates were also characterized by growth at specific salinities or pH gradients, with reference to previous geographical, geological, and climate studies. Unlike the East Sea or West Sea isolates, some Dokdo Islands isolates showed endurable traits with growth in high salinity, and many showed growth under extremely alkaline conditions. A smaller proportion of West Sea coast isolates tolerate compared to the East Sea or Dokdo Islands isolates. These results suggest that these unique fungal biota developed through a close interaction between the host halophyte and their environment, even within the same halophyte species. Therefore, this study proposes the application of specific fungal resources for restoring sand dunes and salt-damaged agricultural lands and industrialization of halophytic plants.

• 농촌지도와개발
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• 제14권2호
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• pp.373-394
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• 2007

The purposes of this study were to explore decision-making patterns of rural couples and to clarify the differences between socio-demographic variables related to the patterns. The data were gathered from 1,870 rural households through interviews with structured questionnaire in the 187 rural communities sampled by multi-staged cluster stratified sampling method in November 2005. A total of 1,106 farm households including householder and spouse participated in the interview. The results of this study were as follows: First, in general, mainly householder made decision-making in the areas of dealing with real estate and selling farm products, while spouse decided in the areas of choosing television channel and deciding living expenses. Second, there were no significant differences of patterns by age, education and income level. Third, decision-making patterns were significantly different by sex and family type. According to the results of the study, in farm household, 'pattern of mainly householder or husband decision-making' was high yet as regarding agricultural decision making. Therefore, researchers suggested that educational program could be helpful for rural couples to participate in practicing gender equity decision- making.