• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.1013-1017
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• 2020

Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) harbor diverse microbial symbionts that play essential roles in host physiology, ecology, and evolution. In this study we aimed to reveal microbial communities associated with two different mealybugs, papaya mealybug (Paracoccus marginatus) and two-tailed mealybug (Ferrisia virgata) collected from the same host plant. Comparative analysis of microbial communities associated with these mealybugs revealed differences that appear to stem from phylogenetic associations and different nutritional requirements. This first report on both bacterial and fungal communities associated with these mealybugs provides a preliminary insight on factors affecting the endomicrobial communities.

• 한국토양비료학회지
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• 제45권6호
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• pp.1073-1085
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• 2012

Soil microbial communities are immensely diverse and complex with respect to species richness and community size. These communities play essential roles in agricultural soil because they are responsible for most of the nutrient cycles in the soil and influence the plant diversity and productivity. However, the majority of these microbes remain uncharacterized because of poor culturability. Next-generation sequencing techniques have revolutionized many areas of biology by providing cheaper and faster alternatives to Sanger sequencing. Among them, amplicon pyrosequencing is a powerful tool developed by 454 Life Sciences for assessing the diversity of complex microbial communities by sequencing PCR products or amplicons. This review summarizes the current opinions in amplicon sequencing of soil microbial communities, and provides practical guidance and advice on sequence quality control, aligning, clustering, OTU- and taxon-based analysis. The last section of this article includes a few representative studies conducted using amplicon pyrosequencing.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1561-1572
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• 2022

Plastic pollution has been recognized as a serious environmental problem, and microbial degradation of plastics is a potential, environmentally friendly solution to this. Here, we analyzed and compared microbial communities on waste plastic films (WPFs) buried for long periods at four landfill sites with those in nearby soils to identify microbes with the potential to degrade plastics. Fourier-transform infrared spectroscopy spectra of these WPFs showed that most were polyethylene and had signs of oxidation, such as carbon-carbon double bonds, carbon-oxygen single bonds, or hydrogen-oxygen single bonds, but the presence of carbonyl groups was rare. The species richness and diversity of the bacterial and fungal communities on the films were generally lower than those in nearby soils. Principal coordinate analysis of the bacterial and fungal communities showed that their overall structures were determined by their geographical locations; however, the microbial communities on the films were generally different from those in the soils. For the pulled data from the four landfill sites, the relative abundances of Bradyrhizobiaceae, Pseudarthrobacter, Myxococcales, Sphingomonas, and Spartobacteria were higher on films than in soils at the bacterial genus level. At the species level, operational taxonomic units classified as Bradyrhizobiaceae and Pseudarthrobacter in bacteria and Mortierella in fungi were enriched on the films. PICRUSt analysis showed that the predicted functions related to amino acid and carbohydrate metabolism and xenobiotic degradation were more abundant on films than in soils. These results suggest that specific microbial groups were enriched on the WPFs and may be involved in plastic degradation.

• 농촌지도와개발
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• 제5권1호
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• pp.1-10
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• 1998

Rural communities have been isolated from social and economic changes underway in the broader society. Trends towed an urbanized society have depopulated many rural areas (Flora, et al, 1992). The South Korean government has targeted efforts toward an urban centered economic development policy, which has neglected the rural areas. The South Korean government also has indoctrinated rural community development with a quick-fix approach. Self-directed economic organizations based in rural South Korea began to emerge in the late 1980s. Since the 1980s, South Korean agricultural sectors have been overshadowed by globalism. McMichael (1996) stated that rural communities have two options. A immunity should either End its niche (i.e. the commodities or products that can have advantages in the international trade market) or protest against globalism to survive if rural communities fail to find a niche under globalism. South Korean neat communities did not have enough natural resources nor enough money to invest for their rural community economic development programs. These limitations made it difficult for South Korean coral communities to compete in a global economic environment. Israel, Coleman, and Ilvento (1993) stated that local leadership is a critical component in the ability of communities to respond to lang-standing problems and emerging needs. Swinth and Alexander (1990) asserted that rural leaders are critical actors in rural community development. Whether rural communities succeed in finding their niche or not, the role of rural community leaders is important for rural communities to find ways to cope in the international agriculture market.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.301-310
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• 2012

Prosopis juliflora and Parthenium hysterophorus are the two arid, exotic weeds of India that are characterized by distinct, profuse growth even in nutritionally poor soils and environmentally stressed conditions. Owing to the exceptional growth nature of these two plants, they are believed to harbor some novel bacterial communities with wide adaptability in their rhizosphere. Hence, in the present study, the bacterial communities associated with the rhizosphere of Prosopis and Parthenium were characterized by clonal 16S rRNA gene sequence analysis. The culturable microbial counts in the rhizosphere of these two plants were higher than bulk soils, possibly influenced by the root exudates of these two plants. The phylogenetic analysis of V1_V2 domains of the 16S rRNA gene indicated a wider range of bacterial communities present in the rhizosphere of these two plants than in bulk soils and the predominant genera included Acidobacteria, Gammaproteobacteria, and Bacteriodetes in the rhizosphere of Prosopis, and Acidobacteria, Betaproteobacteria, and Nitrospirae in the Parthenium rhizosphere. The diversity of bacterial communities was more pronounced in the Parthenium rhizosphere than in the Prosopis rhizosphere. This culture-independent bacterial analysis offered extensive possibilities of unraveling novel microbes in the rhizospheres of Prosopis and Parthenium with genes for diverse functions, which could be exploited for nutrient transformation and stress tolerance in cultivated crops.

• 농촌지도와개발
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• 제16권2호
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• pp.439-461
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• 2009

The aim of this study is to make abolished school utilized to be served for promotion of off-farm incomes, vitality of agriculture and rural communities' economic, i.e. directly related in inhabitants' income. The methods of this study was to classify the types of utilization via analysis of abolished school data base which served by Ministry of Education & Human Resources Development. Moreover, we tried to derive problems of management and the existing system via interview survey of the nation's best 100 executives who utilize abolished school and present active factors of abolished school utilization and improvement directions. Then, we classified types of the idle agricultural land utilization into categories and selected the survey site which were the best on the idle agricultural land. Then, we suggests utilization and improvement directions to the use and management of idle agricultural land for increasing inhabitants' income, vitality of agriculture and rural communities' economic.

• 농촌지도와개발
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• 제7권2호
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• pp.321-325
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• 2000

There are many opportunities for Agricultural Technology Centers and for local communities to benefit from the computer and internet and compete in the information age. However, many rural communities appeared to lack human and financial resources required to enable access to the computer and internet. A case of implementation process of establishing agricultural information system at Asan Agricultural Technology Center during the last 10 years were presented. Extension educators took leadership role in facilitating the educational needs of farmers in terms of related resources for the computer and internet extension. To better prepare communities for the information age, community access was critical for promoting the technology and improving proficiency of local community members in its use, extension educators need to take leadership role in assessing community ability to provide internet access points for people without computers. Extension educators at city/county Agricultural Technology Centers may initiate to attract people with internet needs. computer resources, technological experience, and know-how for setting up internet access points in community locations.

• The Plant Pathology Journal
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• 제37권6호
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• pp.662-672
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• 2021

Plant growth-promoting bacteria improve plant growth under abiotic stress conditions. However, their effects on microbial succession in the rhizosphere are poorly understood. In this study, the inoculants of Bacillus mesonae strain H20-5 were administered to tomato plants grown in soils with different salinity levels (EC of 2, 4, and 6 dS/m). The bacterial communities in the bulk and rhizosphere soils were examined 14 days after H20-5 treatment using Illumina MiSeq sequencing of the bacterial 16S rRNA gene. Although the abundance of H20-5 rapidly decreased in the bulk and rhizosphere soils, a shift in the bacterial community was observed following H20-5 treatment. The variation in bacterial communities due to H20-5 treatment was higher in the rhizosphere than in the bulk soils. Additionally, the bacterial species richness and diversity were greater in the H20-5 treated rhizosphere than in the control. The composition and structure of the bacterial communities varied with soil salinity levels, and those in the H20-5 treated rhizosphere soil were clustered. The members of Actinobacteria genera, including Kineosporia, Virgisporangium, Actinoplanes, Gaiella, Blastococcus, and Solirubrobacter, were enriched in the H20-5 treated rhizosphere soils. The microbial co-occurrence network of the bacterial community in the H20-5 treated rhizosphere soils had more modules and keystone taxa compared to the control. These findings revealed that the strain H20-5 induced systemic tolerance in tomato plants and influenced the diversity, composition, structure, and network of bacterial communities. The bacterial community in the H20-5 treated rhizosphere soils also appeared to be relatively stable to soil salinity changes.

• 농촌계획
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• 제20권4호
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• pp.253-262
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• 2014

This, an association research for design-improvement and model-development of exhibition shops at rural tourism communities, is to secure objective data by analyzing customers' awareness-tendency of and demand for agricultural-specialty exhibition shops. Survey-questions for finding out consumers' awareness-tendency and demand were determined through brainstorming of a professional council, 30 rural communities of which visit-rate by consumers is considerably high were selected for the recruit of 200 consumers. For investigation and analysis, survey and in-depth interview were carried out at the scene with the application of frequency analysis and summarization of their opinions, which revealed that they have a strong will to visit the rural tourism communities for the purchase of agricultural specialties along with the experience of learning-program and on-the-scene direct dealing and that their viewpoint on the direct dealing at the scene was very positive. Also it was confirmed hat their satisfaction with the purchase of agricultural specialties by on-the-scene direct dealing, their pleasure at the purchase, their satisfaction with services and their intention for re-purchase of them were very high while their satisfaction with the exhibition shops was very low. With on-the-scene survey, the consumers' opinions could be listened to in depth. Almost all of them said their satisfaction with the trip to those rural tourism communities was considerably high since they could go to those communities themselves to relieve the stress from their modern life, to experience healing and to see the goods on the scene. Their satisfaction also was attributed to the fact that they have enough trust in purchase along with feeling the warm-heartedness of rural residents. As to their awareness of exhibition shops, they showed a positive response to the on-the-scene direct dealing at rural communities while they, thinking that the space in those exhibition shops was not sufficiently wide, demanded for more systematic counters in more accessible and affordable exhibition shops so that they might be more satisfied with the exhibition shops. Their demand for the necessity of exhibition shops selling agricultural specialties was found to be over 80%, which indicates that the necessity is very high. As to the suitability of function, they have the opinion that the business at those shops had better be focused on sales since they have the understanding of information when they take a trip to the rural communities, while there was another opinion: since agricultural products are seasonal items they should be exhibited and sold at the same time. More than 90% of the respondents had a positive viewpoint on direct dealing of agricultural specialties on the scene, which showed that their response to it was very high. They preferred the permanent shops equipped with roll-around table-booths. In addition, it was revealed that they want systematic exhibition shops in rural communities because they frequent those communities for on-the-scene direct purchase. The preferred type and opinion resulting from estimation of consumers' demands have been reflected for development of practical designs. The structure of variable principles has been designed so that the types of display-case and table-booth might be created. The result of this study is a positive data as a design model which can be utilized at rural communities and will be commercialized for the verification of its validity.

• 한국문헌정보학회지
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• 제12권
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• pp.225-267
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• 1985

This study is aimed to assure that the agricultural technical information which is the important factor to increase rural production should be widely used by rural communities, and for the wide circulation for the technical information, the appropriate information system is available for the farmers and extention workers who are responsible for the information transfer to farmers. The study is also aimed to find out an outline of a structure and characteristic of the appropriate information system for the rural communities in Korea. The method used to accomplish the purpose of the study, the modernization theory of agriculture of T. W. Schultz was examined and assertions of the information specialists regarding the issues of information transfer and agricultural information system :in developing countries were analyzed. Through the above literary research, the author tried to create the most suitable characteristic and structure of agricultural information system in rural communities in Korea. To justify the author's assertion, a survey through questionniares for a typical rural province was attempted. The assertion that the author tried to convey as the most suitable agricultural information system of this country is the establishment agricultural information analysis center mainly for the extention workers and designing information system which has affiliation between the rural guidance office which is already situated every unit of administractive districts all over the country and public library which is now not properly located and functioned.