• Title/Summary/Keyword: Agricultural by Products

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A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-Producing Streptomyces fradiae

  • Zhiming Yao;Jingyan Fan;Jun Dai;Chen Yu;Han Zeng;Qingzhi Li;Wei Hu;Chaoyue Yan;Meilin Hao;Haotian Li;Shuo Li;Jie Liu;Qi Huang;Lu Li;Rui Zhou
    • Journal of Microbiology and Biotechnology
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    • v.33 no.6
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    • pp.831-839
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    • 2023
  • Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by high-performance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 ㎍/ml) and UN-C137 (6889.72 ± 70.25 ㎍/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 ㎍/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

A study on the improvement of distribution system by overseas agricultural investment (해외농업투자에 따른 유통체계 개선방안에 관한 연구)

  • Sun, Il-Suck;Lee, Dong-Ok
    • Journal of Distribution Science
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    • v.8 no.3
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    • pp.17-26
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    • 2010
  • Recently concerns have been raised due to the unbalanced supply of crops: the price of crops has been unstable and at one point the price went up so high that the word Agflation(agriculture+ inflation) was coined. Korea, in particular, is a small-sized country and needs to secure the stable supply of crops by investing in the produce importation at a national level. Investment in foreign produce importation is becoming more important as a measure for sufficient supply of crops, limited supply of domestic crops, weakened farming conditions worldwide, as well as recent changes in the use of crops due to the development of bio-fuels, influence of carbon emission on crops, the price increase in crops, and influx of foreign hot money. However, there are many problems with investing in foreign produce importation: lack of support from the government; lack of farming information and technology; difficulty in securing the capital; no immediate pay-off from the investment and insufficient management. Although foreign produce is originally more price-competitive than domestic produce, it loses its competiveness in the process of importation (due to high tariffs) and poor distribution system, which makes it difficult to sell in Korea. Therefore, investment in foreign produce importation is being questioned for feasibility; to make it possible, foreign produce must maintain the price-competitiveness. Especially, harvest of agricultural products depends on natural and geographical conditions of each country and those products have indigenous properties, so distribution system according to import and export of agricultural products should be treated more carefully than that of other industries. Distribution costs are differentiated into each item and include cost of sorting and wrapping, cost of wrapping materials, cost of domestic transport, cost of international transport and cost of clearing customs for import and export. So transporting and storing agricultural products generates considerable costs compared with other products. Also, due to upgrade of dietary life, needs for stability, taste and visible quality toward food including agricultural products are being raised and wrong way of storage causes decomposition of food and loss of freshness, making the storage more difficult than that in room temperature, so storage and transport in distribution of agricultural products needs specialty. In addition, because lack of specialty in distribution and circulation such as storage and wrapping does not solve limit factors in distance, the distribution and circulation has been limited to a form of import and export within short-distant region. Therefore, need for distribution out-sourcing which can satisfy specialty in managing distribution and circulation and it is needed to establish more effective distribution system. However, existing distribution system of agricultural products is exposed to various problems including problems in distribution channel, making distribution and strategy for distribution and those problems are as follows. First, in case of investment in overseas agricultural industry, stable supply of the products is difficult because areas of production are dispersed widely and influenced by outer factors due to including overseas distribution channels. Also, at the aspect of quality, standardization of products is difficult, distribution system is quite complicated and unreasonable due to long distribution channels according to international trade and financial and institutional support is not enough. Especially, there are quite a lot of ineffective factors including multi level distribution process, dramatic gap between production cost and customer's cost, lack of physical distribution facilities and difficulties in storage and transport due to lack of wrapping containers. Besides, because import and export of agricultural products has been manages under the company's own distribution according to transaction contract between manufacturers and exporting company, efficiency is low due to excessive investment in fixed costs and lack of specialty in dealing with agricultural products causes fall of value of products, showing the limit to lose price-competitiveness. Especially, because lack of specialty in distribution and circulation such as storage and wrapping does not solve limit factors in distance, the distribution and circulation has been limited to a form of import and export within short-distant region. Therefore, need for distribution out-sourcing which can satisfy specialty in managing distribution and circulation and it is needed to establish more effective distribution system. Second, among tangible and intangible services which promote the efficiency of the whole distribution, a function building distribution environment which includes distribution information, system for standard and inspection, distribution finance, system for diversification of risks, education and training, distribution administration and tax system is wanted. In general, such a function building distribution environment is difficult to be changed and supplement innovatively because its effect compared with investment does not appear immediately despite of its necessity. Especially, in case of distribution of agricultural products, as a function of collecting and distributing is performed individually through various channels, the importance of distribution information and standardization is getting more focus due to the problem of repetition of work and lack of specialty. Also, efficient management of distribution is quite difficult due to lack of professionals in distribution, so support to professional education is needed. Third, though effort to keep self-sufficiency ratio of staple food, rice is regarded as important at the government level, level of dependency on overseas of others crops is high. Therefore, plan for stable securing food resources aside from staple food is also necessary. Especially, governmental organizations of agricultural products distribution in Korea are production-centered and have unreasonable structure whose function at the aspect of distribution and consumption is quite insufficient. And development of new distribution channels which can deal with changes in distribution environment and they do not achieve actual results of strategy for distribution due to non-positive strategy for price distribution. That is, it implies the possibility that base for supply will become vulnerable because it does not mediate appropriate interests on total distribution channels such as manufacturers, wholesale dealers and vendors by emphasizing consumer protection excessively in the distribution of agricultural products. Therefore, this study examined fundamental concept and actual situation for our investment to overseas agriculture, drew necessities, considerations, problems, etc. of overseas agricultural investment and suggested improvements at the level of distribution for price competitiveness of agricultural products cultivated in overseas under five aspects; government's indirect support, distribution's modernization and distribution information function's strengthening, government's political support for distribution facility, transportation route, load and unloading works' improvement, price competitiveness' securing, professional manpower's cultivation by education and training, etc. Here are some suggestions for foreign produce importation. First, the government should conduct a survey on the current distribution channels and analyze the situation to establish a measure for long-term development plans. By providing each agricultural area with a guideline for planning appropriate production of crops, the government can help farmers be ready for importation, and prevent them from producing same crops all at the same time. Government can sign an MOU with the foreign government and promote the importation so that the development of agricultural resources can be stable and steady. Second, the government can establish a strategy for an effective distribution system by providing farmers and agriculture-related workers with the distribution information such as price, production, demand, market structure and location, feature of each crop, and etc. In order for such distribution system to become feasible, the government needs to reconstruct the current distribution system, designate a public organization for providing distribution information and set the criteria for level of produce quality, trade units, and package units. Third, the government should provide financial support and a policy to seek an efficient distribution channel for foreign produce to be delivered fresh: the government should expand distribution facilities (for selecting, packaging, storing, and processing) and transportation vehicles while modernizing old facilities. There should be another policy to improve the efficiency of unloading, and to lower the cost of distribution. Fourth, it is necessary to enact a new law covering exceptional cases for importing produce in order to maintain the price competitiveness; currently the high tariffs is keeping the imported produce from being distributed domestically. However, the new adjustment should be made carefully within the WTO regulations since it can create a problem from giving preferential tariffs. The government can also simplify the distribution channels in order to reduce the cost in the distribution process. Fifth, the government should educate distributors to raise the efficiency and to modernize the distribution system. It is necessary to develop human resources by educating people regarding the foreign agricultural environment, the produce quality, management skills, and by introducing some successful cases in advanced countries.

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Influence of various levels of milk by-products in weaner diets on growth performance, blood urea nitrogen, diarrhea incidence, and pork quality of weaning to finishing pigs

  • Yoo, S.H.;Hong, J.S.;Yoo, H.B.;Han, T.H.;Jeong, J.H.;Kim, Y.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.696-704
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    • 2018
  • Objective: This study was conducted to evaluate various levels of milk by-product in weaning pig diet on growth performance, blood profiles, carcass characteristics and economic performance for weaning to finishing pigs. Methods: A total of 160 weaning pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc), average $7.01{\pm}1.32kg$ body weight (BW), were allotted to four treatments by BW and sex in 10 replications with 4 pigs per pen in a randomized complete block design. Pigs were fed each treatment diet with various levels of milk by-product (Phase 1: 0%, 10%, 20%, and 30%, Phase 2: 0%, 5%, 10%, and 15%, respectively). During weaning period (0 to 5 week), weaning pigs were fed experimental diets and all pigs were fed the same commercial feed during growing-finishing period (6 to 14 week). Results: In the growth trial, BW, average daily gain (ADG), and average daily feed intake (ADFI) in the nursery period (5 weeks) increased as the milk by-product level in the diet increased (linear, p<0.05). Linear increases of pig BW with increasing the milk product levels were observed until late growing period (linear, p = 0.01). However, there were no significant differences in BW at the finishing periods, ADG, ADFI, and gain:feed ratio during the entire growing-finishing periods. The blood urea nitrogen concentration had no significant difference among dietary treatments. High inclusion level of milk by-product in weaner diet decreased crude protein (quadratic, p = 0.05) and crude ash (Linear, p = 0.05) of Longissimus muscle. In addition, cooking loss and water holding capacity increased with increasing milk product levels in the weaner diets (linear, p<0.01; p = 0.05). High milk by-product treatment had higher feed cost per weight gain compared to non-milk by-products treatment (linear, p = 0.01). Conclusion: Supplementation of 10% to 5% milk by-products in weaning pig diet had results equivalent to the 30% to 15% milk treatment and 0% milk by-product supplementation in the diet had no negative influence on growth performance of finishing pigs.

Marketing and Improvement of Environmental-Friendly Agricultural Products in Jeonnam (전남의 친환경농산물 유통실태 및 개선방안)

  • Song, Kyung-Hwan;Kim, Byung-Moo
    • Korean Journal of Organic Agriculture
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    • v.12 no.3
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    • pp.303-316
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    • 2004
  • Recently, with the improvement of national income, the increase of int e rest in the consumers, health, and the enhancement of recognition of environment the demands of environmental-friendly agricultural products(EFAPs) are on the rise rapidly. The demand in the market for EFAPs increases rapidly, and the sales amount increases by 30~40% every year. But, it is in situation that the EFAPs arc not distributed well between the producers and consumers now. That is, the producers of EFAPs can not End th e ir markets. And, the consumers do not trust the EFAPs. Also, it is in situation that it is difficult to promote the demand because of relatively higher prices than the general agricultural products. To solve these problems, it may be summed up as follows. First, it needs to reduce the distribution cost by the construction of stable supply basis. Second, it needs to improve the distribution system of EFAPs. Third, it needs to enhance the differentiation and improve the consumers, reliability. Fourth, it needs to make in brand and extend the various publicities. Fifth, it needs to construct the supporting system and enhance the cooperation with the related institutions. Sixth, it needs to End new demand and introduce the procurement system by the government.

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Application of Electronic Nose in Discrimination of the Habitat for Special Agricultural Products (특용작물의 산지판별을 위한 전자코 응용)

  • Noh, Bong-Soo;Ko, Jae-Won;Kim, Sang-Yong;Kim, Su-Jeong
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1051-1057
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    • 1998
  • The discrimination of the agricultural origin, especially locally produced or imported from the products such as Ganoderma lucidum, sesame and arrowroot were investigated by using the electronic nose. Volatile components from these products were discriminated by twelve of conducting polymer sensors without any pretreatment. Pattern recognition was carried out. Multiple discriminant analysis showed the difference between imported agricultural product and locally produced ones such as Ganoderma lucidum, sesame and arrowroot. Unknown habitat of sesame and arrowroot could be identified by multiple discriminant analysis whether the imported or the locally produced one.

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Monitoring and risk assessment of pesticide residues in school foodservice agricultural products in Incheon (인천광역시 학교급식 농산물의 잔류농약 실태조사 및 위해성 평가)

  • Park, Byung-Kyu;Kwon, Sung-Hee;Yeom, Mi-Sook;Han, Se-Youn;Kang, Min-Jung;Seo, Soon-Jae;Joo, Kwang-Sig;Heo, Myung-Je
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.470-478
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    • 2021
  • This study was conducted to monitor residual pesticides in a total of 527 school foodservice agricultural products from 2019 to 2020 in Incheon. Pesticide residues in the samples were analyzed by the multi-residue method in the Korean food code for 373 pesticides using GC-MS/MS, LC-MS/MS, GC-ECD, GC-NPD, and HPLC-UVD. By monitoring the pesticides, 12 (2.3%) of the 527 pesticides were detected, and 2 (0.4%) samples exceeded the maximum residue limit. Twelve types of pesticides were detected in the agricultural products of carrot, chard, chili pepper, chwinamul, crown daisy, parsley, perilla leaves, and spinach. As a means of risk assessment through the consumption of agricultural products detected with pesticide residues, the proportion of estimated daily intake to acceptable daily intake was estimated in the range of 0.0000-39.7425%. Results showed no particular health risk through the consumption of school foodservice agricultural products with pesticide residues.

Silage from Agricultural By-products in Thailand: Processing and Storage

  • Suksombat, W.;Lounglawan, P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.4
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    • pp.473-478
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    • 2004
  • Processing and storage of the silage from agricultural by-products were studied in two experiments. The first experiment was conducted to investigate the chemical composition and degradability of various silages with varying ensiling times. The experiment was a 5$\times$3 factorial design, completely randomized, with factor A as the different formulated mixtures by varying level of urea addition (0, 0.5, 1.0, 1.5 and 2.0%) and factor B as the time of ensiling. Chemical composition changed little with time and varied only slightly with levels of urea in the mixtures. Dry matter (DM) degradability increased with increasing cassava levels while crude protein degradability and pH level increased with increasing urea addition. By using 'Flieg point', which relates to organic acid yields, there were no significant difference among ensiled mixtures and times of ensiling. Therefore it can be concluded that the 5th silage formulation is the most appropriate since its DM and crude protein (CP) degradability were highest. The second experiment was carried out to determine the quality of the 5th silage mixtures (from the previous trial) after being stored for up to 6 months. The experiment was a complete randomized design with samples taken at monthly intervals up to 6 months and subjected to laboratory and degradability analyses. The results showed no significant (p>0.05) difference in chemical composition except for increased neutral detergent fiber and acid detergent fiber percentage in association with increasing storage time. There were no significant (p>0.05) differences in 'Flieg point' among times of storage. In conclusion, this experiment showed that the silage from agricultural by-products can be stored for more than 6 months.

Enzymatic Synthesis of Novel $\alpha$-Amylase Inhibitors via Transglycosylation by Thermotoga maritima Glucosidase

  • Kim, Sung-Hee;Lee, Myoung-Hee;Yang, Sung-Jae;Kim, Jung-Woo;Cha, Hyun-Ju;Cha, Jae-Ho;Nguyen, Van Dao;Park, Kwan-Hwa
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.302-307
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    • 2008
  • Novel amylase inhibitors were synthesized via transglycosylation by Thermotoga maritima glucosidase (TMG). TMG hydrolyzes acarbose, acarviosine-glucose, and maltooligosaccharide by releasing $^{14}C$-labeled glucose from the reducing end of each molecule. When TMG was incubated with acarviosine-glucose (the donor) and glucose (the acceptor), two major transfer products, compounds 1 and 2, were formed via transglycosylation. The structures of the transfer products were determined using thin-layer chromatography (TLC), high-performance ion chromatography (HPIC), and $^{13}C$ nuclear magnetic resonance (NMR) spectroscopy. The results indicate that acarviosine was transferred to glucose at either C-6, to give a $\alpha-(1{\rightarrow}6$) glycosidic linkage, or at C-3, to produce an $\alpha-(1{\rightarrow}3$) glycosidic linkage. The transfer products showed a mixed-type inhibition against porcine pancreatic $\alpha$-amylase; therefore, they may be useful not only as inhibitors but also as acarbose transition-state analogs to study the mechanism of amylase inhibition.

Establishment of monitoring system by ubiquitous computing (유비쿼터스 컴퓨팅에 의한 모니터링 시스템 구축)

  • Lee, Yun-Min;Shin, Jin-Seob
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.19 no.1
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    • pp.127-132
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    • 2019
  • In this paper, we design and build an information system for the growth management of agricultural products and aquatic products by ubiquitous computing. So that, we installed various sensor systems and developed IP Cam to establish monitoring. It was possible to construct an environmental management system based on IT technology by a database that directly controls and manages the growth conditions of agricultural products and aquatic products by monitoring. We have installed various sensors that can acquire data in real time and IP Cam is designed and manufactured in three parts. It was applied to agricultural and marine products and monitored soil moisture, temperature, and environmental information to establish conditions for growth environment.

The Effect of Purchasing Factors of Environment-Friendly Agricultural Products on Consumer Attitude and Purchasing Intention (친환경농산물의 구매요인이 소비자태도와 구매의도에 미치는 영향)

  • Lee, Jong-Ho
    • Culinary science and hospitality research
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    • v.22 no.4
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    • pp.204-221
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    • 2016
  • The study tested the moderating effect of 215 women living in Busan to analyze the purchase intention (awareness, health interests, environmental interests) of environment-friendly agricultural products and it's the causal relationship between consumer attitudes and purchasing intention. The results confirmed that all purchase factors for environment-friendly agricultural products had significant causality on consumer attitudes. Significant causality of consumer attitudes on purchase intention was also confirmed. The moderating effect of the number of purchases was analyzed to only significantly affect consumer attitudes on purchase intention and consumer attitudes according to number of purchases were analyzed to vary depending on the intensity of purchase. Information presented in this study means that consumers who are aware of environment-friendly agricultural products, interested in health, and interested in protecting the environment exhibit positive attitudes on environment-friendly agricultural products as well as higher purchase intentions. Therefore, if government and local organizations strengthen the promotion of environment-friendly agricultural products, there may be an acceleration of the consumption of environment-friendly agricultural products, followed by an increase in the income of farmers as well as an increase in the number of farmers who cultivate environment-friendly agricultural products. This may ultimately aid in the conservation of the natural environment of Korea and enhance the health and well-being of the public.