• 한국균학회지
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• 18권2호
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• pp.77-83
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• 1990

사철느타리버섯과 느타리버섯의 영양요구주로 원형질체 융합과정을 관찰하고, 융합에 영향을 미치는 여러 가지 요인을 분석한 결과는 다음과 같다. 원형질체에 polyethylene glycol을 혼합하였을 때 원형질체는 서로 응집되어 원형질막의 접착이 이루어졌으며, 0.6 M sucrose로 PEG를 제거한 후에 두 개 또는 다수의 원형질체가 융합되었다. 원형질체 융합에 크게 영향을 주는 요인은 PEG 농도, pH, 삼투압조정제였으며 원형질체의 농도, PEG 흔합시간, 원형질체 배양온도 및 시간, PEG 분자량, $CA^{2+}$, glycine도 영향을 주었다. 가장 알맞는 조건을 pH 8.0으로 조절된 30% PEG 8000으로 원형질체에 혼합하여 $30^{\circ}C$에서 10분간 배양하고 0.6 M sucrose에 현탁하여 동일한 삼투압조정제가 첨가된 배지에서 배양될 때 가장 많은 융합주를 획득할 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권1호
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• pp.13-17
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• 2009

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Cordyceps militaris, C. pruinosa and Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori) host were used in this examination. This study was carried out to investigate the soluble sugar, amino acid and fatty acid profiles in the fruiting-bodies. Soluble sugars such as glycerol, glucose, mannitol and sucrose were mainly found in the fruiting bodies of C. militaris, C. pruinosa and P. tenuipes. Total soluble sugar content was 29.23 mg/g in C. militaris, 8.61 mg/g in C. pruinosa and 24.00 mg/g 1in P. tenuipes on dry weight basis. Total free amino acid content was 14.09 mg/g 1in C. militaris, 34.60 mg/g in C. pruinosa and 17.09 mg/g in P. tenuipes. The content of oleic acid in fatty acids was above high more than 30% regardless of species.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.117-117
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• 2020

• 드라이브 ㆍ 컨트롤
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• 제19권4호
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• pp.46-53
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• 2022

RIn this study, a test tractor that could measure various types of agricultural operational loads was developed, and its performance was verified. This tractor could be used to measure the load generated during agricultural work and convert the related data into a database. A test tractor was developed using an 80-kW-rated load tractor, and it could measure various types of field test data, such as engine torque and rpm, wheel torque, PTO(power take-off) torque, hexometer, IMU/INS sensor, steering angle sensor, hydraulic pressure, and flow sensor data. To verify the developed test tractor, a verification test using an agriculture rotavator was performed. The test conditions were L1, L2, and L3 based on the tractor's main and sub-transmission stages, and stages 1 and 2 were selected as the PTO. In a comparison of the analyzed test data, similar tendencies in the test results of this research and other research (Kim's research) were seen. Through this, the developed test tractor was verified. In the future, we plan to conduct research on the tractor developed in this study using various attached working machines.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.319-321
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• 1989

• 한국잠사곤충학회지
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• 제48권1호
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• pp.16-20
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• 2006

동충하초 재배 농가는 누에가 고치를 짓게 한 후 동충하초균 감염 개체를 식별하기 위하여 그 고치를 절개하는 작업 노력이 수반되나, 고치를 짓지 않는 나용 1대 교잡종을 이용함으로써 고치를 절개하는 노력을 줄일 수 있다. 나용 교잡종을 이용할 때에는 사육온도와 균 접종조건을 기존의 방법과는 달리해야 한다는 것이 다음과 같이 확인되었다. (1) 동충하초 생산성을 높이는 나용 교잡종 사육 온도(가) 나용화율 표준 사육온도($^{\circ}C$)보다 $3^{\circ}C$ 고온사육 때 나용화율이 약 90%로. 가장 높았고, $-2^{\circ}C$ 저온사육 때 약 40%로 가장 낮았다. (나) 용화율 및 용체중 : 고온과 적온사육 간에는 큰 차이가 없었으나 저온 사육에서는 다소 낮아지는 경향이었다. (다) 누에 소잠에서 상족까지 소요일수 : 표준사육 온도에 비해 고온사육에서 3.3일 단축되고 저온사육에서 5.7일 연장되었다. (2) 나용 교잡종에 대한 동충하초균의 최적 접종 조건(가)균 접종 농도 : $1{\times}10^7spores/ml$에서 감염을 및 분생자병속 형성율이 가장 높았고, $5{\times}10^7spores/ml,\;1{\times}10^8spores/ml$ 순으로 접종농도가 높아질수록 감염률 및 분생자병속 형성율이 낮아졌다. (나) 접종시간 : 춘추 모두 12시간 고온 다습처리 메 감염을 및 분생자병속 형성률이 높았다.

• 농촌계획
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• 제9권3호
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• pp.41-46
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• 2003

The study was focused on setting priority for future core technologies in rural development and resources using AHP. The technologies were derived by Delphi method. Evaluation criteria for the priority setting were decided as 'technology', 'market oriented', and 'public concerns' by council. The future core technologies were divided as four groups by importance and R&D level. Technologies in upper two groups were considered in the study. Group I had high importance and high R&D level. Group II had high importance and lower R&D level. Questionnaires were given to 9 specialists in agricultural resources. As the results, 'public concerns' was decided as a most important evaluation criterion. The most important technologies are "technology developing for animal feces and urine treatment, and restoration it to farm land" in group I, and "technology developing for agricultural by-products as energy source" in group II.

• 기술혁신학회지
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• 제2권3호
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• pp.107-117
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• 1999

This study was conducted to provide a improving direction of R&D investment for agricultural technology. The results is summerized as follows : 1) it is necessary to make a systematic master-plant of R&D investment for agricultural technology especially including management system 2) Reinforcement of researchers and investment related with agricultural technology might be im-portant factor 3) To clarify the roles by organization is necessary to efficient management for this is needed to efficient use of research resource 5) In the meeting pro-vice era a synthetic management of tech-nology developed by researchers and mutual exchange between research organization researcher is necessary. Finally Builing up a information delivery system of agricultural technology compatible to use the technology developed is recommended.

• 고려인삼연구와산업
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• 제13권1호
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• pp.3-10
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• 2019

• Mycobiology
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• 제36권1호
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• pp.50-54
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• 2008

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by $^{13}C-NMR$. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By $^{13}C-NMR$ analysis, the immuno-stimulating substance was identified as ${\beta}-(1{\rightarrow}3)\;(1{\rightarrow}6)$-glucan, composed of a backbone with $(1{\rightarrow}3)$-linked D-glucopyranosyl residues branching a $(1{\rightarrow}6)$-linked D-glucopyranosyl residue. The ${\beta}$-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.