• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.70-76
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• 1999

Since high concentrations of N, P, and organic C cause the excessive eutrophication in water systems, the control of nutrient export from agricultural nonpoint sources has become important. This study was conducted to estimate discharges of N, P, and organic C from a small agricultural watershed of the upper Imgo stream in Youngchun, Kyongbuk. Of the total area(1.420ha), 25% was agricultural land including paddy, upland and orchards and most of the remainder was forest. The resident population in the watershed was 194 in 80 households and relatively small numbers of livestocks including cow were raised. Mean concentrations of nutrients in the stream water were 4.95, 0.80, 6.72, 0.07 and 2.52mg/L for $NO_3-N$, $NH_4-N$, Total N, Total P and COD respectively. Annual discharges in 1997 were 28,991kg of $NO_3-N$. 3,010kg of $NH_4-N$, 37,006kg of Total N. 590kg of Total P, and 29,138kg of COD. There was a strong positive relationship between stream flow and precipitation, and also most of the nutrient discharges occurred in the rainy season (May to August). Since there was no any other industries in the watershed, agricultural practices and sewage from the resident households, forest runoff and livestock wastes were the major sources of NPS discharges. A combination of management options, including management of soil erosion and fertilizer application, could lead to reductions in nutrient exports.

• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1295-1300
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• 2009

A 2,172-bp full-length gene (aga-F78), encoding a protease-resistant $\alpha$-galactosidase, was cloned from Rhizopus sp. F78 and expressed in Escherichia coli. The deduced amino acid sequence shared highest identity (45.0%) with an $\alpha$-galactosidase of glycoside hydrolase family 36 from Absidia corymbifera. After one-step purification with a Ni-NTA chelating column, the recombinant Aga-F78 migrated as a single band of ~82 and ~210 kDa on SDS-PAGE and nondenaturing gradient PAGE, respectively, indicating that the native structure of the recombinant Aga-F78 was a trimer. Exhibiting the similar properties as the authentic protein, purified recombinant Aga-F78 was optimally active at $50^{\circ}C$ and pH 4.8, highly pH stable over the pH range 5.0-10.0, more resistant to some cations and proteases, and had wide substrate specificity (pNPG, melidiose, raffinose, and stachyose). The recombinant enzyme also showed good hydrolytic ability to soybean meal, releasing galactose of $415.58\;{\mu}g/g$ soybean meal. When combined with trypsin, the enzyme retained over 90% degradability to soybean meal. These favorable properties make Aga-F78 a potential candidate for applications in the food and feed industries.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.254-263
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• 2020

This study developed indexes of farmers' acceptance factors for new farming technologies and evaluated the importance of the developed. For this study, farming technologies were classified according to the criteria established by the Rural Development Administration, and the Delphi technique was applied to evaluate the importance of the indexes. The indexes were composed of two tiers and evaluated by the analytic hierarchy process (AHP). Results showed that the importance of the first tier was finance and profitability with 43.9%, technology affairs 23.6%, labor environments 22.4%, and related industries and service system 10.1%. The importance of the second tier was the ease of technology acceptance 64.9% and technology completion 35.1% for technology affairs in the first tier, labor intensity 53.7%, work hazard 26.7%, working hours 19.6% for labor environment, farm income increment 69.9% and new fund required 30.1% for finance and profitability, and training and service system 66.7% and activation of related industries 33.7% for related industry and service system. This study may contribute to farmers' acceptance of new farming technologies and increase the efficiency and effectiveness of agricultural research and extension activities by the Rural Development Administration.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.718-731
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• 2018

The beneficial effects of lactic acid bacteria (LAB) have been intensively investigated in recent decades with special focus on modulation of the host intestinal microbiota. Numerous discoveries of effective probiotics are driven by a significantly increasing demand for dietary supplements. Consequently, technological advances in the large-scale production and lyophilization are needed by probiotic-related industries for producing probiotic LAB for commercial use. Our study had a dual objective, to determine the optimum growth medium composition and to investigate appropriate cryoprotective additives (CPAs) for Lactobacillus salivarius, and compare its responses with other Lactobacillus species. The one-factor-at-a-time method and central composite design were applied to determine the optimal medium composition for L. salivarius cultivation. The following composition of the medium was established (per liter): 21.64 g maltose, 85 g yeast extract, 1.21 ml Tween 80, 6 g sodium acetate, $0.2g\;MgSO_4{\cdot}7H_2O$, $0.02g\;MnSO_4{\cdot}H_2O$, $1g\;K_2HPO_4$, $1.5g\;KH_2PO_4$, $0.01g\;FeSO_4{\cdot}7H_2O$, and 1 g sodium citrate. A cryoprotective additive combination comprising 10% (w/v) skim milk and 10% (w/v) sucrose supplemented with 2.5% (w/v) sodium glutamate was selected for L. salivarius, and its effectiveness was confirmed using culture-independent methods in the freeze-dried cells of the Lactobacillus strains. In conclusion, the optimized medium enhanced the species-specific cultivation of L. salivarius. On the other hand, the cryoprotective effects of the selected CPA mixture may also be dependent on the bacterial strain. This study highlights the necessity for precise and advanced processing techniques for large-scale production of probiotics in the food and feed industries.

• Journal of Biosystems Engineering
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• v.37 no.4
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• pp.258-264
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• 2012

Purpose: This review aims at introducing 3 modeling approaches classified into 3 categories based on the purpose (estimation or prediction), structure (linear or non-linear) and phase (steady-state or dynamic-state); 1) statistical approaches, 2) kinetic modeling and 3) mechanistic modeling. We hope that this review can be a useful guide in the model-based approach of calcium metabolism as well as illustrates an application of engineering tools in studying biosystems. Background: The meaning of biosystems has been expanded, including agricultural/food system as well as biological systems like genes, cells and metabolisms. This expansion has required a useful tool for assessing the biosystems and modeling has arisen as a method that satisfies the current inquiry. To suit for the flow of the era, examining the system which is a little bit far from the traditional biosystems may be interesting issue, which can enlarge our insights and provide new ideas for prospective biosystem-researches. Herein, calcium metabolic models reviewed as an example of application of modeling approaches into the biosystems. Review: Calcium is an essential nutrient widely involved in animal and human metabolism including bone mineralization and signaling pathways. For this reason, the calcium metabolic system has been studied in various research fields of academia and industries. To study calcium metabolism, model-based system analyses have been utilized according to the purpose, subject characteristics, metabolic sites of interest, and experimental design. Either individual metabolic pathways or a whole homeostasis has been modeled in a number of studies.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.3
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• pp.378-381
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• 2001

Sulfamethazine has been widely used in swine for prevention or treatment of infections. Recently, the safety of the drug to consumers has been questioned because of carcinogenic effects. To prevent unwanted drug residues entering the human food chain, both government authorities and industries have established extensive control measures. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing nowadays. In this study, we established a rapid prediction test for the detection of cattle with violative tissue residues of sulfamethazine. The recommended therapeutic dose of sulfamethazine (withdrawal time, 15 days) was administered to each of 10 cattle. Blood was sampled before drug administration and during the withdrawal period. The concentration of sulfamethazine in plasma, determined by a semi-quantitative ELISA, was compared to that of an internal standard (10 ppb). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive and if larger than 1 was considered negative. All 10 plasma samples from non-treated cattle showed negative to sulfamethazine. Sulfamethazine was detected in plasmas of treated cattle until Day 7 of withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting residues of sulfamethazine in live cattle.

• International Journal of Contents
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• v.9 no.4
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• pp.22-29
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• 2013

In this paper, we propose a integrated real-time monitoring system for recycling agriculture resourcing based on USN. We design and implement the monitoring system so that we can integrate the quality control of farmyard and liquid manures, barn environment monitoring, and positioning information control into a total management system performing recycling of excrement and manure. Selection of sensors and sensor-node construction and requirements, structure of wire/wireless communication networks, and design of monitoring program are also presented. As a result of operating our system, we can get over various drawbacks of conventional separated system and promote the proper circulation of excrement up to the farmyard. We confirm that these advanced effects arise from the effective management of the total system integrating quality control of farmyard/liquid manure, barn/farmhouse information, and vehicle moving monitoring information etc. Moreover, this monitoring system is able to exchange real-time information throughout communication networks so that we can construct a convenient information environment for agricultural community by converging IT technology with farm and stockbreeding industries. Finally we present some results of processing using our monitoring system. Sensing data and their graphs are processed in real-time, positioning information on the v-world map offers various moving paths of vehicles, and statistical analysis shows all the procedure from excrement occurrence to recycling and resourcing.

• Journal of The Korean Society of Agricultural Engineers
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• v.56 no.3
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• pp.11-17
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• 2014

We investigated the applicability of steel slag as a capping material in order to minimize phosphorus(P) release into seawater. Steel slag is a byproduct from the iron and steel industries and the use of steel slag has some advantages in respect of both cost and environmental concern. P removal by steel slag were studied in a batch system with respect to changes in contact time and initial concentration. Kinetic adsorption data were described well by pseudo 2nd order model, indicating rate limiting step for P adsorption to steel slag is chemical sorption. Equilibrium adsorption data fitted well to Langmuir isotherm model which describes for single layer adsorption. The maximum P adsorption capacity of steel slag was 7.134 mg-P/L. Increasing the depth of steel slag produced a positive effect on interruption of P release. More than 3 cm of steel slag was effective for blocking P release and 5 cm of steel slag was recommended as the depth for capping of P contaminated marine sediments. Increasing P concentration and flow rate had a negative effect on P removal ratio. It was concluded that the steel slag has a potential capping material for blocking P release from marine sediments.

• Korean Journal of Weed Science
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• v.17 no.1
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• pp.94-111
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• 1997

A relatively wide range of weed technology concerning sound herbicide development, its formulation exploitation, and application techniques was extensively reviewed in accordance with least inputted sustainable agriculture. Herbicide plays an integral part of farmers' cultural practices in Korean agriculture like rest of world over. Weeds are more obstacle to the adaptation of more sustainable agriculture system. Because weeds dictate most of the crop production practices, weed scientists must become the leaders of collaborative integrated approaches to agriculture systems research. Feasible ways to minimize herbicide input involve the positive introduction of low-rate selective chemistries, innovative formulations, biological herbicides and newly developed application technology. Since herbicide will remain to be a core position in weed management for the foreseeable future, research is needed to optimize herbicide application technology and to minimize their impact on the environment. Public concerns and regulatory pressures on agricultural chemicals are likely to strengthen throughout this decade and coming. Researchers, pesticide manufacturing industries and regulatory authorities have to work together more closely, leading to understand each other better, as well as the needs of our customers and those of society. To be taken extensively, weed control management practices in the future have to be accepted such a way the respective demands of environment, society and economics are entirely matched.