• 한국지하수토양환경학회지:지하수토양환경
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• 제26권6호
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• pp.165-175
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• 2021

Groundwater is used in many areas in food industry such as food manufacturing, food processing, cooking, and liquor industry etc. in Korea. As groundwater occupies a large portion of food industry, it is necessary to predict deterioration of water quality to ensure the safety of food water since using undrinkable groundwater has a ripple effect that can cause great harm or anxiety to food users. In this study, spatiotemporal data aggregation method was used in order to obtain spatially representative data, which enable prediction of groundwater quality change in a small watershed. In addition, a highly reliable predictive model was developed to estimate long-term changes in groundwater quality by applying a non-parametric segmented regression technique. Two pilot watersheds were selected where a large number of companies use groundwater for food water, and the appropriateness of the model was assessed by comparing the model-produced values with those obtained by actual measurements. The result of this study can contribute to establishing a customized food water management system utilizing big data that respond quickly, accurately, and preemptively to changes in groundwater quality and pollution. It is also expected to contribute to the improvement of food safety management.

• Biomolecules & Therapeutics
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• 제31권3호
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• pp.285-297
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• 2023

Alzheimer's disease (AD) is a neurodegenerative disease with progressive memory loss and the cognitive decline. AD is mainly caused by abnormal accumulation of misfolded amyloid β (Aβ), which leads to neurodegeneration via a number of possible mechanisms such as down-regulation of brain-derived neurotrophic factor-tropomyosin-related kinase B (BDNF-TRKB) signaling pathway. 7,8-Dihydroxyflavone (7,8-DHF), a TRKB agonist, has demonstrated potential to enhance BDNF-TRKB pathway in various neurodegenerative diseases. To expand the capacity of flavones as TRKB agonists, two natural flavones quercetin and apigenin, were evaluated. With tryptophan fluorescence quenching assay, we illustrated the direct interaction between quercetin/apigenin and TRKB extracellular domain. Employing Aβ folding reporter SH-SY5Y cells, we showed that quercetin and apigenin reduced Aβ-aggregation, oxidative stress, caspase-1 and acetylcholinesterase activities, as well as improved the neurite outgrowth. Treatments with quercetin and apigenin increased TRKB Tyr516 and Tyr817 and downstream cAMP-response-element binding protein (CREB) Ser133 to activate transcription of BDNF and BCL2 apoptosis regulator (BCL2), as well as reduced the expression of pro-apoptotic BCL2 associated X protein (BAX). Knockdown of TRKB counteracted the improvement of neurite outgrowth by quercetin and apigenin. Our results demonstrate that quercetin and apigenin are to work likely as a direct agonist on TRKB for their neuroprotective action, strengthening the therapeutic potential of quercetin and apigenin in treating AD.

• 지능정보연구
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• 제29권4호
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• pp.229-256
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• 2023

팬데믹으로 인한 재택근무와 가정용 전력수요의 증가는 전력수요 패턴에 상당한 변화를 불러왔다. 이로 인해 한전 PPA(전력구매계약) 및 자가용 태양광 발전량 파악이 어려워지고, 전력거래소의 전력수요예측과 계통운영에 어려움이 가중되고 있다. 전기에너지는 다른 에너지 자원과 달리 저장이 어려워, 생산된 에너지와 소비 사이의 균형을 유지하는 것이 매우 중요하다. 전기에너지의 부족이나 과잉 생산은 에너지 시스템에 큰 불안정성을 초래할 수 있으므로, 전력 수급을 효과적으로 관리하는 것이 필수적이다. 특히, 4차 산업혁명 시대에는 데이터의 중요성이 더욱 커져 대규모 화재나 정전과 같은 문제가 심각한 영향을 미칠 수 있다. 이에 따라, 전기에너지 분야에서 정확한 전력수요와 함께 재생에너지와 같은 발전량을 정확하게 예측하여 적절한 발전 관리를 하는 것이 중요하며, 이는 불필요한 전력 생산을 줄이고 에너지 자원을 효율적으로 활용하는데 도움이 된다. 이에, 본 연구에서는 산업통상자원부에서 제공한 169개 발전소의 데이터를 활용하여 최적의 집합전력자원을 구성하기 위해 (1) 재생에너지 발전량 예측제도와 목표, 그리고 실제 적용에 대해 검토하고, (2) 예측제도 정산을 고려한 집합구성 알고리즘을 개발한 후, (3) 분석 로직에 이를 적용하여 결과를 종합하고 해석하였다. 본 연구는 최적의 집합구성 알고리즘을 개발하여, 최대 정산금 대비 80.66%에 달하는 집합구성(Result_Number 546)을 도출하였으며, 발전소 집합을 구성하였을 때 정산금을 증가시키는 발전소(B1783, B1729, N6002, S5044, B1782, N6006)와 정산금을 감소시키는 발전소(S5034, S5023, S5031)를 확인하였다. 집합전력자원을 연구단위로 설정하여 최적의 집합구성 알고리즘을 개발한 최초의 연구로서 의의가 있으며, 본 연구결과의 활용으로 전력시스템의 안정성을 향상시키고 에너지 자원이 효율적으로 활용될 수 있기를 기대한다.

• 원예과학기술지
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• 제32권1호
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• pp.84-90
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• 2014

우리나라에서 먹넌출은 안면도 지역에서만 소나무 숲에서 제한적으로 분포하는 덩굴성 식물이다. 본 연구는 먹넌출 집단의 분포형태와 특성, 유전다양성 및 공간분포에 따른 유전구조를 파악하는데 있다. 선발된 8개 I-SSR primer에서 총 50개의 I-SSR 증폭산물을 얻었으며 37개의 단형성 증폭산물을 제외한 13개의 다형적 증폭산물을 분석에 이용하였다. 공간적 자기상관 분석을 위한 조사구 $90m{\times}70m$내에 총 39개체의 먹넌출이 자생하고 있었으며, 군집지수(aggregation index)는 0.706으로 집중분포(clumped distribution)하는 공간분포를 나타냈다. I-SSR 표지자 분석 결과 39개체 중 유전자형이 서로 다른 21개의 유성생식체(genet)가 식별되었으며, 유전자형 비율(G/N)은 53.8%, 유전자형 다양성(D)은 0.966, 유전자형 균등도(E)는 0.946으로 각각 나타났다. Shannon의 다양성지수(I = 0.598)는 적은 개체수와 제한적 분포에도 불구하고 다른 수종들에 비해 비교적 높은 유전다양성을 나타냈다. Tanimoto distance를 이용한 공간적 자기상관 분석 결과 안면도 먹넌출의 현지외 보존을 위한 표본 추출 전략은 6m 이상의 간격을 두고 개체를 선발하는 것이 타당한 것으로 나타났다.

• 대한치과보철학회지
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• 제17권1호
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• pp.47-59
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• 1979

In order to study the morphologic changes of the unfixed odontoblasts suspended in phenol solution of several different concentrations, the author carried out the extraction of lower incisor of S-D strain rats to collect the odontoblasts, and the cells obtained were suspended immediately in saline solution. After observing the odontoblasts in fresh state, the saline solution was substituted with 0.125%, 0.25% 0.5%, 1% and 2% diluted phenol solutions. The morphologic changes were examined with phase contrast microscope at intervals of 10, 30, and 60 minutes. The results were as follows: 1. In saline solution the odontoblast showed cytoplasmic swelling, slender cytoplasmic process, thick rim nuclear membrane with increased dark contrast, and prominent nucleoli and chromatin granules with lapse of time intervals. In accordance with time intervals, blisters appeared in the supranuclear zone and increased its size and moved outward of the cytoplasmic membrane resulting detachment from the cell membrane. The phase dark cytoplasmic granules were increased in its dark contrast and in its size. 2. In 0.125% and 0.25% phenol solution, the odontoblasts and its nucleus shrunk immeidately and its contrast of cellular components was increased. With the lapse of time, the phase-dark granules in cytoplasm were aggregated, and several blisters were formed in and out of the cells. The outline of cytoplasmic membrane was also obscured. 3. In 0.5% phenol solution, the necleus shrunk at once, but soon after it revealed karyolysis accompanying dark contrast of neclear components such as nuclear membrane, nucleoli, and chromatin granules. On the contrary, the cytoplasmic granules showed aggregation and increased dark contrast, small and large blisters were formed in and out of the odontblasts and the outline of cytoplasmic membrane became obscured. 4. In 1% phenol solution, it showed shrinkage of odontblasts and its nuclei with thick rim nuclear membrane, aggregation of chromatin granules and occasional karyorrhexis. The dark contrast of cytoplasmic granules was increased and aggregated each other. But the blister formation could not be found. 5. In 2% phenol solution, it showed the shrinkage of odontoblasts and pyknotic nuclei with increased dark contrast of nucleoli and chromatin granules. The number of cytoplasmic granules was decreased by aggregation. But the blister formation could not be found as in 1% phenol solution.

• 한국정보과학회논문지:데이타베이스
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• 제34권6호
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• pp.503-517
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• 2007

본 논문에서는 부분 집계 근사법(Piecewise Aggregation Approximation: PAA)이 MBR-안전(MBR-safe) 성질을 가짐을 보이고, 이를 사용한 효율적인 서브시퀀스 매칭 방법을 제안한다. MBR-안전 변환이란 고차원 MBR을 직접 변환한 저차원 MBR이 개별 고차원 시퀀스가 변환된 저차원 시퀀스를 모두 포함하는 변환을 의미한다. 이와 같은 MBR-안전 변환을 사용하면 고차원 MBR을 직접 저차원 MBR로 변환할 수 있어 유사 시퀀스 매칭에서 필요한 저차원 변환 횟수를 크게 줄일 수 있다. 또한, PAA는 계산이 간단하고 성능이 우수한 저차원 변환으로 알려져 있다. 이에 따라, 본 논문에서는 이들 두 개념의 장점을 통합하기 위하여, 기존의 PAA가 MBR-안전 성질을 가짐을 확인하고, 이를 사용하여 서브시퀀스 매칭의 성능을 개선한다. 본 논문의 공헌은 다음과 같다. 첫째, PAA 기반의 MBR 저차원 변환인 mbrPAA를 제안하고, mbrPAA가 MBR-안전함을 정형적으로 증명한다. 둘째, mbrPAA 기반의 새로운 서브시퀀스 매칭 방법을 제안하고, 이 방법의 정확성을 증명한다. 셋째, 서브시퀀스 매칭에서 엔트리 재사용 성질(entry reuse property)의 개념을 제시하고, 이 개념에 기반하여 고차원 MBR을 효율적으로 구성하는 방법을 제안한다. 넷째, 실험을 통해 mbrPAA의 우수성을 입증한다. 실험 결과, 제안한 mbrPAA는 기존 방법에 비해 저차원 MBR 구성을 평균 24.2배 빠르게 수행하고, 서브시퀀스 매칭 성능을 최대 65.9%까지 향상시킨 것으로 나타났다.

• 한국응용곤충학회지
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• 제48권1호
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• pp.67-72
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• 2009

콩 포장에서 톱다리개미허리노린재의 집합페로몬이 노린재검정알벌에 대하여 kairomone 으로서 미치는 영향을 조사하였다. 노린재검정알벌의 기생률은 페로몬 트랩과 톱다리개미허리노린재 알의 위치가 0에서 15 m로 거리가 멀어질수록 감소하였고, 노린재검정알벌의 유인거리는 약 15 m였다. 콩 포장조건에서 노린재검정알벌의 밀도는 8월 하순과 9월 상순에 최대 발생시기를 보였고, 페로몬 트랩 설치수를 $165\;m^2$ 당 1개에서 3개로 증가함에 따라 기생률이 증가하였다. 그러나 약제처리구에서 기생률이 비교적 매우 낮았다. 노린재검정알벌의 포장밀도에 대한 성비조사에서 암컷이 더 많이 유인되었다.

• The Plant Pathology Journal
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• 제33권4호
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• pp.393-401
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• 2017

Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudomonas oleovorans strain KBPF-004 (developmental code KNF2016) which showed antiviral activity against mechanical transmission of tobamoviruses. Antiviral activity was also evaluated in seed transmission of two tobamoviruses, Pepper mild mottle virus (PMMoV) and Cucumber green mottle mosaic virus (CGMMV), by treatment of seed collected from infected pepper and watermelon, respectively. Pepper and watermelon seeds were treated with culture supernatant of P. oleovorans strain KBPF-004 or control strain ATCC 8062 before planting. Seeds germinated after treatment with water or ATCC 8062 yielded about 60% CGMMV or PMMoV positive plants, whereas < 20% of KBPF-004-treated seeds were virus-infected, a significantly reduced seed transmission rate. Furthermore, supernatant of P. oleovorans strain KBPF-004 remodeled aggregation of PMMoV 126 kDa protein and subcellular localization of movement protein in Nicotiana benthamiana, diminishing aggregation of the 126 kDa protein and essentially abolishing association of the movement protein with the microtubule network. In leaves agroinfiltrated with constructs expressing the coat protein (CP) of either PMMoV or CGMMV, less full-size CP was detected in the presence of supernatant of P. oleovorans strain KBPF-004. These changes may contribute to the antiviral effects of P. oleovorans strain KBPF-004.

• 약학회지
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• 제46권1호
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• pp.11-17
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• 2002

$\beta$-Immunan, a proteoglycan, was isolated from the mycelium of Canoderma lucidum which belongs to a medicinal mushroom. The effects of $\beta$-Immunan on cell-cell and cell-matrix interactions mediated by carbohydrate-recognition and the mechanism responsible for the inhibition of experimental metastasis of Bl6-F10 and B16/BL6 murine melanoma were studied. The results showed that $\beta$-Immunan inhibited Bl6-Fl melanoma cell's adhesion to laminin and asialofetuin-induced homotypic aggregation and reduced invasion against Bl6-F10 murine melanoma cells through matrigel in vivo assay. When $\beta$-Immunan was intraperitoneally administrated to C57B/6 mice bearing B16/BL6 murine melanoma cells, it was decreased the number of pulmonary metastatic colony by the dose dependent manner ranging from 20 to 100 mg/kg/day. The results indirectly indicate that clinical treatment with $\beta$-Immunan might be expected to exhibit anti-metastatic effect. In the pulmonary metastasis, the number of pulmonary metastatic colony of melanoma when $\beta$-Immunan was intraperitoneally administrated to C57BL/6 mice bearing B16/BL6 murine melanoma cells by intravenous injection were decreased by the dose dependent manner ranging from 20 to 100 mg/kg/day.

• 통상정보연구
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• 제11권1호
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• pp.485-515
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• 2009

In this study aims to research and analyze what are decision factors between a wholly-owned subsidiary and a international company cooperation in the internationalization process. From 57 primary studies found 26 observed variables for aggregation of effect size in the using "Fixed Effect Model" on this mata-analysis. The article also links the theories that are used in the literature, not considered in the most of primary studies, to the empirical results and recommends global management strategy which is needed for business practical affairs. According to the result, the meta-analysis suggests that the number of employees of a MNC, the geological and cultural differneces between the host country and home country, the country-specific international experience of the MNC and export intensity, the international product diversification, the market growth and the market size of the foreign operations, and resource intensity of the foreign operations, the legal restrictions in the host country and the country risk of the home country do exert a significant effect on the decision between cooperative arrangement and a wholly-owned subsidiary. Conversely, for a large number of variables, no significant relationship seems to exist based on the combined results. This is the case, for example, for factor specificity, the r&d-intensity, the subsidiary size, the assets of the MNC and the sales volume of the MNC.