• International Journal of Oral Biology
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• v.43 no.2
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• pp.69-76
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• 2018

Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelialmesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.171-180
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• 2005

Background & Objective : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether Puerariae radix could induce angiogenic activity in human umbilical vein endothelial cells (HUVECs). Methods: The angiogenic activity of Puerariae radix were evaluated by using BrdU assay, chemotactic migration assay, tube formation assay, measurement of bFGF in HUVECs, and Matrigel plug assay in mice. Results : Puerariae radix significantly increased HUVECs proliferation in a dose-dependent manner. In addition, Puerariae radix increased migration and tube-like formation in HUVECs. Interestingly,the expression of basic fibroblast growth factor (bFGF), an angiogenesis-stimulating growth factor, was dose-dependently increased by Puerariae radix. The angiogenic activity of Puerariae radix was confirmed using an in vivo Matrigel angiogenesis model, showing promotion of blood vessel formation. Conclusion : Puerariae radix significantly induces angiogenesis in vitro and in vivo. These results suggest that Puerariae radix is a potent angiogenic agent, and a promising drug, for the induction of neovascularization.

• Journal of Ginseng Research
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• v.43 no.3
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• pp.488-495
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• 2019

Background: Timosaponin AIII (TA3) is a steroidal saponin extracted from Anemarrhena asphodeloides. Here, we investigated the anticancer effects of TA3 in MG63 human osteosarcoma cells. TA3 attenuates migration and invasion of MG63 cells via regulations of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, which are involved with cancer metastasis in various cancer cells. TA3 reduced enzymatic activities and transcriptional expressions of MMP-2 and MMP-9 in MG63 cells. TA3 also inhibited Src, focal adhesion kinase, extracellular signal-regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, ${\beta}-catenin$, and cAMP response element binding signaling, which regulate migration and invasion of cells. TA3 induced apoptosis of MG63 cells via regulations of caspase-3, caspase-7, and poly(ADP-ribose) polymerase (PARP). Then, we tested several ginsenosides to be used in combination with TA3 for the synergistic anticancer effects. We found that ginsenosides Rb1 and Rc have synergistic effects on TA3-induced apoptosis in MG63 cells. Methods: We investigated the anticancer effects of TA3 and synergistic effects of various ginseng saponins on TA3-induced apoptosis in MG63 cells. To test antimetastatic effects, we performed wound healing migration assay, Boyden chamber invasion assays, gelatin zymography assay, and Western blot analysis. Annexin V/PI staining apoptosis assay was performed to determine the apoptotic effect of TA3 and ginsenosides. Results: TA3 attenuated migration and invasion of MG63 cells and induced apoptosis of MG63 cells. Ginsenosides Rb1 and Rc showed the synergistic effects on TA3-induced apoptosis in MG63 cells. Conclusions: The results strongly suggest that the combination of TA3 and the two ginsenosides Rb1 and Rc may be a strong candidate for the effective antiosteosarcoma agent.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.05b
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• pp.907-910
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• 2003

본 논문에서는 에이전트의 효율적 이주를 제공하기 위한 전역 디렉토리 스킴을 제안한다. 제안된 스킴은 동적으로 삯은 변경을 갖는 분산 시스템에서 에이전트의 서비스 수행을 위한 에이전트 및 환경에 대한 트래킹을 통해 효율적인 이동 에이전트의 이주를 지원하는 것을 목적으로 한다. 본 연구에서는 이를 위해 대표적인 이동에이전트 시스템인 IBM's Aglets Software Development Kit을 확장하여 에이전트 수행 환경에 대한 모니터링 모듈을 구현하고 이를 기반으로 기존의 마스터-슬래이브 형태의 이동 에이전트 시스템을 확장하여 구현하였다.

• Proceedings of the Korea Multimedia Society Conference
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• 2004.05a
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• pp.851-854
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• 2004

분산 유전 알고리즘은 외판원 문제를 해결하는데 효율적이고 적용하기 쉬운 알고리즘이다. 그러나 다중 후보해를 가진 분산 유전 알고리즘을 수행할 때, 효율성과 정확성에 영향을 주는 많은 요소들이 고려되어야 한다. 후보해의 크기를 얼마로 할 것인지, 이주의 비율 및 횟수는 어떻게 할 것인지와 그리고 어떤 개체들을 선택해서, 어떤 후보해 개체와 교환할 것인가가 중요하게 고려되어야 한다. 따라서 본 논문에서는 이주해야 할 개체의 크기를 동적으로 변경하면서 교환하는 방법과 또한 개체들이 이주되어야 할 위치를 결정하는 이주 정책을 개발하고 실험하였다.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.644-647
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• 2003

Materials currently used as an injectable bulking agent in the dermatologic and urologic fields revealed several drawbacks such as particles migration, inflammatory reaction, allergic reaction, rapid volume shrinkage, and necessity of a donor site. In this study, we have developed injectable biomaterial comprising poly (DL- lactide-co-glycolide)(PLGA) and hyaluronic acid gel to overcome these problems. PLGA is a biocompatible synthetic material and hyaluronic acid is a common substance found in living organisms. We examined the feasibility of injection through needle and tested biocompatibility in animal model. After transplantation, injected sites and distant organs were examined histologically to verify a new tissue formation, inflammation, and particles migrations. Injected volume was maintained approximately 80 percent for 2 months. Results demonstrated that the developed material was injectable through various gauges of needles and induced a new bulking tissue formation without serious inflammatory reaction.

• International Journal of Control, Automation, and Systems
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• v.2 no.3
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• pp.333-342
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• 2004

This paper presents a framework for the self-organization of swarm systems based on coupled nonlinear oscillators (CNOs). In this scheme, multiple agents in a swarm self-organize to flock and arrange themselves as a group using CNOs, which are able to keep a certain distance by the attractive and repulsive forces among different agents. A theoretical approach of flocking behavior by CNOs and a design guideline of CNO parameters are proposed. Finally, the formation scenario for cooperative multi-agent groups is investigated to demonstrate group behaviors such as aggregation, migration, homing and so on. The task for each group in this scenario is to perform a series of processes such as gathering into a whole group or splitting into two groups, and then to return to the base while avoiding collision with agents in different groups and maintaining the formation of each group.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.3B
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• pp.314-324
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• 2004

Recently, high-speed internet subscribers have grown continuously, and reached up to 10 millions. But existing network management that is responsible only for user access is confronted with many difficulties, since user access network(xDSL, Cable, B-WILL, and so on) has diversified and value added wired/wireless contents service has developed. In this paper propose the Agent based Inter Networking platform as object-oriented hierarchical system that can provide various service such as access QoS(Quality of Service), customized application service and security service according to user on demand. This system has divided into two parts(user agent and agent management system ＆ local and central management systems). Management system periodically receive status information such as bandwidth, download speed, delay, current service, and so on, and process them, then build a policy based on processed information. According to personal user profile that is generated by this policy, the customized service can be provided for users. And This system adopt the object-oriented modeling in case that system migration and integration, and implement a system that is robust and stable for security through hierarchical server system.

• The KIPS Transactions:PartD
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• v.8D no.4
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• pp.439-450
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• 2001

Mobile agent is one of the promising ways of overcoming network load and latency. It is also a new software paradigm including those concepts of client/server, applet/servelet, and code-on-demand. In this paper, a new mobile agent concept with flexible replay scheme is proposed, which can deal with embarrassing situation when replying results should be accomplished in various distributed applications. For example, they are 1) a burden of bulky result, 2) a need of different replay scheme dependent on work type, 3) connection failure to the target node, and 4) a need of fast migration to next node to keep the itinerary. Regarding the source node, there may be another situation that it wants to be aware of whether its wi가 is completed or not. Three kinds of reply schemes are proposed for dealing flexibly with such situations. They are based on priority reply list where nodes to be replied are stored according to their priorities. An experimental supply chain management model using the proposed reply schemes is designed and implemented. It is one of good distributed applications appropriate for our reply schemes, because it requires different reply schemes according to work types and it is important to gather required information in time.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1332-1338
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• 2018

In the course of studies to discover natural products with anti-angiogenic properties, two cyclic octapeptides, octaminomycins A (1) and B (2), were isolated from the cultures of Streptomyces sp. RK85-270. Octaminomycins suppressed the vascular endothelial growth factor (VEGF)-induced proliferation, adhesion, tube formation, migration, and invasion of HUVECs. Anti-angiogenic activity was futher confirmed in vivo by the chicken chorioallantoic membrane assay. We also identified that 1 and 2 inhibited the phosphorylation of VEGF receptor 2, AKT, and ERK1/2 and the expression and activities of MMP-2 and MMP-9. These results suggest that 1 and 2 may serve as potential scaffolds for the development of therapeutic agents to angiogenesis-dependent diseases.