• 한국환경보건학회지
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• 제19권1호
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• pp.30-36
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• 1993

A survey was conducted to measure concentration of airborne microbe in a hospital using RSC air sampler during October~November 1991.The result was as follows: 1) In an agar strip GK-A media for total counts of microbial particles. The highest count were 1384 CFU/m$^3$ in the main lobby, followed by 912 CFU/m$^3$, in the obstetric room, 688 CFU/m$^3$ in 1CU. By gram staining, the distribution for organisms in the air were shown 74.1% in gram possitive cocci followed by 16.8%, in gram possitive bacilli 6.7% in gram negative bacilli and 4.7% in yeast, but low organism was detected in recovery room with 194 CFU/m$^3$. 2) In agar strip S media for Staphylococci the count at the main lobby was detected in the recovery room with 92 CFU/m$^3$, Tests of coagulase were negative Staphylococci with 78%, and positive Staphylococci with 22%. The Staphylococci were highly resistance to penicillin, ampicillin and sensitive to amikacin, cefazolin, gentamycin and chloramphenicol. 3) In agar strip C media for coliform bacteria the colony counts at the main lobby was 139 CFU/m$^3$ and treatment room was 190 CFU/m$^3$, most frequently isolated microorganisms were non fermentative bacilli. 4) In agar strip HS media for yeast and molds. Most frequently colony counts 17~76 CFU/m$^3$, 0.5% lactophenol cotton blue stains were shown unidentified 77.2%, 8.1%, in Penicillium 8.1% in Aspergillus, and 3.8% in mucor.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2002년도 춘계 국제 학술대회
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• pp.3-5
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• 2002

A measurement of indoor microorganism using Reuter Centrifugal Air Sampler(RCS) was undertaken during October 1991-February 1999 and 6-Stage Cascade Air Sampler was undertaken during May 2001-June 2001 in Seoul. The results were as follows : 1. The average of total microorganism collected on the agar strip GK-A media were, in the order, subway station, hospital, underground shopping center, department store, book store, theater, sports facility, educational institution, office building in sites. 2. The average of staphylcocci collected on the agar strip GK-S media, in the order, were subway station, underground shopping center, hospital and department store, department store, theater, office building, sports facility and educational institution in sites. 3. The average of fungus collected on the agar strip GK-HS media, in the order, were Underground hospital, shopping center, theater, subway station, department store, book store, sports facility, educational institution, and office building in sites

• 한국산업보건학회지
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• 제10권1호
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• pp.115-125
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• 2000

Three major hospitals with over 500 beds located in and near Seoul were surveyed for airbone microorganisms from February 1, 1998 to February 18, 1998. The purpose of the study was to identify and quantify microbiological organisms circulating in the air of three different areas in the hospitals. For the study, a RCS air sampler was utilized equipped with two different collection media, the agar strip GK-A for bacteria and the agar strip HS for fungi. The areas investigated were the intensive care unit (ICU) in the Department of internal medicine, the Newborns room in the Department of Obstetrics, and the microbiology laboratory. The results were as follows; 1. The average numbers of general microbiological particles collected on the agar strip GK-A media were $205CFU/m^3$, $232CFU/m^3$, and $128CFU/m^3$ in each hospitals. The highest concentration of $387CFU/m^3$ was found in the ICU of A hospital at 15:00 during the day. Further analysis of the collected bioaerosols by gram staining, revealed that there were gram positive cocci (89.5%), gram positive bacilli (7.2%), gram negative bacilli (2.8%), and fungi (0.5%), in descending order of frequency. 2. Ten different genes were identified from the agar strip GK-A. The most frequently identified organisms were: the Coagulase negative staphylococcus (55.0%), Micrococcus (21.4%), Enterococcus species(10.4%), and Bacillus species (7.2%). A series of antibiotics susceptibility test were conducted against the aforementioned four(4) organisms. Ninety percent of coagulase negative stapylococcus were sensitive to Penicillins. Pathogenic microbes isolated include: Staphylococcus aureus, Acinetobacter species, Klebsiella pneumonia, Klebsiella oxytoca, and Pseudomonas aeroginosa. 3 Although 56.8% of the microorganisms grown on the strip HS media for fungi could not be identified, some of them were successfully identified. The most frequently found fungi were Aspergillus (35.3%), Yeast or Molds (6.2%), and Penicillium (0.7%). Based on the results obtained from the study, it was concluded that some areas in the hospitals had abnormally high bioaerosol concentrations which could be attributed to human activity. Therefore, it is recommended that periodic assessments of indoor bioaerosols aiming to identify the possible sources should be conducted in order to maintain clean indoor environment in the hospitals.

• 한국환경보건학회지
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• 제28권1호
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• pp.85-92
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• 2002

A measurement of indoor microorganism using Reuter Centrifugal Air Sampler(RCS) was undertaken during October 1991 - February 1999 and 6-Stage Cascade Air Sampler was undertaken during May 2001 - June 2001 in Seoul. Sites including book store, department store, theater, subway station, underground shopping center, hospital, office building, sports facility, and eduationa institutio were chosen to measure indoor microorganism. The results were as follows: 1. The average of total microorganism collected on the agar strip GK-A media were, in the order, subway station, hospital, underground shopping center, department store, book store, theater, sports facility, educational institution, office building in sites. The highest concentration of 711cfu/m$^3$ was found in the subway station and the lowest concentration of 44cfu/m$^3$ was found in office building. 2. The average of staphylococci collected on the agar strip GK-S media, in the order, were subway station, underground shopping center, hospital and department store, department store, theater, office building, sports facility and educational institution in sites. The highest concentration of 502cfu/m$^3$ was found in the subway station and the lowest concentration of 14cfu/m$^3$ was found in sports facility and educational institution. 3. The average of fungus collected on the agar strip GK-HS media, in the order, were underground hospital, shopping center, theater, subway station, department store, book store, sports facility, educational institution, and office building in sites. The highest concentration of 252cfu/m$^3$ was found in the hospital and the lowest concentration of 32cfu/m$^3$ was found in office building. 4. Ratio of Indoor/Outdoor, determined by site was 1.12-2.38 in total count, 1.00-2.35 in staphylococci, and 0.99-1.34 in fungus. 5. The positive results of test were 12-24% in indoor and 9-43% in outdoor. 6. By gram staining gram positive cocci were 59.9%, gram positive bacill 24.4%, gram negative bacilli 10.4%, and gram negative cocci 0.5%.

• 대한의생명과학회지
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• 제2권1호
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• pp.49-55
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• 1996

대장균군 검사용 간이 시험지는 본 실험실에서 국내 최초로 고안, 개발하였으며 이 간이 시험지법은 현장 검사법의 하나로 대장균군이 내는 succinic acid dehydrogenase 때문에 tetrazolium salt가 환원되어 적색 반점을 형성하는 것을 이용한 방법으로서 이 간이 시험지의 제조는 대체로 종래의 표준 평판법과 거의 동일한 조성의 배지와 시약을 사용하여 여지에 흡착시킨 후, 건조시켜($60^\circ$C) 멸균한 것으로 표준 평판법과 어떤 상관관계가 있는가를 검토하였다. 이 간이 시험지의 제조에서는 bile salt No. 3를 deoxycholate로 대체하여 제조 원가를 절감하였고, 또한 일본에서 현재 시판되고 있는 제품과 품질 비교시험을 하여 더 좋은 결과를 얻었으며 종래의 표준 평판법과 비교하였을 때도 오히려 표준 평판법 (24-48시간 배양)보다 빠른 시간(16-20시간 배양)내에 판정할 수 있는 이 점이 있으며, 표준 평판법에서는 없어서는 안될 배지나 배양 접시, pipette등의 자료 및 기구가 일체 필요없고 언제 어디서나 현장에서 직접 시험할 수 있어 매우 간편하며 또한 저렴한 가격으로 제조 할 수 있는 경제성이 높은 이점을 갖고 있다.

• 대한수의학회지
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• 제37권2호
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• pp.359-365
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• 1997

Staphylococcus hyicus subsp. hyicus strains isolated from pigs, chickens and cattle were examined for the production of staphylokinase after inhibition of staphylococcal proteases by two procedures with EDTA(disodium). In one, EDTA was added to the bovine fibrin-dog plasminogen agar medium in concentration of 0.07% and paper strips soaked in 2mg/ml soy bean trypsin inhibitor were then applied on the agar plates. In the other, paper strips soaked in 5% EDTA solution were applied on the bovine fibrin-dog plasminogen agar plates and the strains to be tested were then streaked at right angles with the strip. By these procedures, staphylokinase activity was detected in 8(88.9%) of 9 strains from diseased pigs and in 57(80.3%) of 71 strains from skin of healthy pigs, but not in any strains from skin of healthy chickens and milk samples of mastitic cattle. Additionally kinase activity in 9 Staphylococcus species and subspecies isolated from bovine intramammary infections was also tested by these procedures. Staphylokinase activity was detected in 74.2% of Staph aureus strains and in 25% of Staph xylosus strains.

• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.599-602
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• 1997

Fluctuation of fungal zoospores on agar strips were observed in the rumen of sheep fed three different levels of dietary concentrate, timothy hay: concentrate = 3:0 (AF diet), timothy hay: concentrate = 2:1 (MC diet), timothy hay : concentrate = 1:2 (HC diet) respectively. The number of zoospores on the strip was drastically decreased after morning feed with AF diet. The number was the highest at 0 h ($1.34{\times}10^2/cm^2$), then declined to $2.0{\times}10^3/cm^2$ at 9 h after feeding. In the rumen of animals fed MC diet, the number of zoospores decreased with time after feeding, although the decrement was slower than that with AF diet. During 0-3 h after feeding, number of zoospores was $1.6{\times}10^4/cm^2$. Although the number slightly decreased at 6 and 9 h, relatively high levels were maintained. It seems that the inducers for zoospore-release were maintained at relatively high concentration throughout incubation period. The fluctuation pattern of number of germinated zoospores was different in the rumen of animals fed HC diet from those of AF and MC diets. The number of zoospores was constantly maintained at lower level ($1.0{\times}10^3/cm^2$) than the other diets. For MC diet, continuous high number of germinated zoospores may be due to the continuous release of zoospores by hemes in timothy hay and concentrate feed, and by unknown mechanisms. Unlike AF diet which promoted relatively rapid decline of zoosporogenesis, supplementation of concentrate feed to the timothy hay did not promote such rapid decline of zoosporogenesis. It was suggested that release of inducers for zoosporogenesis from concentrate feed persisted longer time than from timothy hay. HC diet promoted the lowest zoospore production, suggested the lowest fungal population size in this experiment. These results show that an appropriate amount of concentrate may support fungal growth and stimulate zoosporogenesis in the rumen.

• 대한미생물학회지
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• 제21권1호
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• pp.1-16
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• 1986

Fifty-one strains of Pseudomonas aeruginosa were isolated from various clinical specimens. Among them, 26 (51%) strains were gentamicin-resistant (Gm') and 25 (49%) were susceptible to gentamicin (Gm'). The frequencies of resistant strains to piperacillin (Pi), cefotaxime, moxalactam, cefoperazone (Cz), and amikacin (Ak) ranged from 21.6 to 31.4%, and $MIC_{50}$ of these drugs were lower than the critical concentrations of susceptibility and resistance. Thirty (58.8%) strains were multiply resistant to 12 or more drugs. All Gm' strains were multiply resistant to 12 or more drugs and one was resistant to all 18 drugs tested, while only four Gm' strains were multiply resistant to 12 drugs and the multiplicity of resistance of the other Gm' strains were less than 10 drugs. Resistance to Gm appeared to have a significant correlation with the resistance to tobramycin (Tb), Ak, Pi, and Cz. All Gm' strains were resistant to Tb and about 38.4 to 46.1% of them were resistant to Ak, Pi, and Cz. The incorporation of $Ca^{++}$ and $Mg^{++}$ ions in Mueller-Hinton agar (MHA) did not influence the MICs of Gm, Tb, carbenicillin (Cb), Pi, and Cz as compared with the results obtained in MHA without these ions. Gm strains were studied on the combined effect of beta-lactam antibiotics and aminoglycosides by the methods of checkerboard and modified paper strip diffusion. Most Gm' strains showed significant synergistic effects by the FIC index between Ak and three beta-lactam antibiotics; Cb, Pi, and Cz, but these results did not in agreement the results obtained through the method of modified paper strip diffusion test. In order to know the nature of the drug resistance of P. aernginosa, the plasmid profile analysis was studied. Agarose gel electrophoresis of lysates processed by the method of Kado and Liu showed one or more plasmids in 22 (43.1%) strains. A group of 19 strains showed at least one band of plasmid and three strains two bands. The range of the molecular weight of plasmids was 3.8 to 243 Mdal. All strains carrying large plasmids larger than 200 Mdal were isolated from wound specimens. Three Gm' strains also harboured the plasm ids of 13 to 203 Mdal.

• 대한소아치과학회지
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• 제37권2호
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• pp.186-192
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• 2010

Streptococcus mutans는 구강 내에 상존하는 치아우식증의 주요 원인균으로서 치면의 피막에 부착 후 glucan을 형성하여 세균의 군락을 이루며, 외부로부터 공급된 자당대사를 통하여 유기산을 생성함으로써 법랑질을 탈회시킨다. 치아우식 활성도의 평가를 위한 단클론항체를 이용한 방법은 진료실에서 빠른 시간 내에 간편하게 타액에 존재하는 Streptococcus mutans의 정량분석이 가능한 방법이다. 이 연구는 3세에서 6세 사이의 어린이 15명을 대상으로 자극성 타액을 채취하여 시판 중인 단클론항체를 이용한 Salivacheck$^{TM}$ Mutans, strip을 이용한 Dentocult$^{(R)}$-SM 그리고 MSB배지 배양법으로서 타액 내 Streptococcus mutans를 측정한 후 그 값을 우식경험치아수와 비교하여 상관관계를 알아보았다. Saliva-check$^{TM}$ Mutans를 이용한 방법은 Dentocult$^{(R)}$-SM과 MSB배지법과 통계학적으로 유의한 상관관계를 보였으나(p<0.05), MSB배지법은 어린이의 우식경험치아수와 통계학적으로 유의한 결과를 나타내지 않았다(p=0.34).

• 한국식품과학회지
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• 제45권1호
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• pp.133-136
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• 2013

본 연구에서는 소시지와 야채 샐러드에서 Y. enterocolitica의 검출을 위해 배지법과 real-time PCR법을 비교하였다. 소시지와 야채 샐러드에 Y. enterocolitica를 접종하고 PSBB에서 증균배양 하였으며, CIN agar에서 선택배양하였다. 동시에 증균배양액에서 1 mL을 채취하여 real-time PCR을 실시하였다. 실험결과, real-time PCR은 소시지에서 배지검출법과 비교하여 동일한 검출력을 보였으나 야채 샐러드에서는 훨씬 더 많은 양성을 검출하였다(p<0.05). 결론적으로 real-time PCR은 식품 시료와 관계 없이 표준 검출법인 배지검출법과 비교하여 동등하거나 우수한 민감도를 지닌 신속검출기법인 것으로 사료되며, 배지검출법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 있어서 매우 유효한 방법이 될 것으로 판단된다.