• 제목/요약/키워드: Aflatoxins

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식품중의 Aflatoxins - 분석방법 및 이화학적 반응을 통한 저감화를 중심으로 - (Aflatoxins in Foods - Analytical methods and Reduction of Toxicity by Physicochemical Processes -)

  • 황준호;전향숙;이광근
    • Applied Biological Chemistry
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    • 제47권1호
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    • pp.1-16
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    • 2004
  • 이 논문에서는 식품 또는 식품원료 중의 aflatoxin에 관한 오염현황, 최신 분석법 그리고 저감화 시킬 수 있는 방안에 대해 조명하였다. Aflatoxin은 여러 곰팡이가 생성하는 2차 대사산물로서 곰팡이 독소 중 가장 독성이 강한 것으로 알려져 있다. 발암성을 포함한 독성 관계로 aflatoxin은 인간의 건강 측면 뿐 아니라 식품을 포함한 산업전반에 경제적으로 큰 영향을 끼치고 있다. 우리나라와 같은 식량 수입국에서는 식품의 안전성 확보를 위해 노출량 조사, 위해평가 및 관리가 체계적으로 이루어져야 하며 아울러 새로운 aflatoxin 분석 기술의 개발과 독성 저감화 기술의 개발이 병행되어야 한다고 판단된다.

TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구 (The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals)

  • 한양일;김광호;오영복
    • 한국환경보건학회지
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    • 제5권1호
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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실험실 폐수중 Aflatoxin 감소를 위한 화학적 처리에 관한 연구 (Chemical Treatment for the Destruction of Aflatoxins in Laboratory Waste Water)

  • 김종규
    • 한국환경보건학회지
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    • 제18권2호
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    • pp.52-56
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    • 1992
  • The ability of chemicals, 10% sodium hypochlorite, 28% ammonium hydroxide, 5% sodium hydroxide, 5% sodium bicarbonate, 0.1% hydrochloric acid, 5% hydrogen peroxide, and 5% acetone, to destroy aflatoxins in laboratory waste water containing 3.26 ppb of B$_{1}$ 7.64 ppb of B$_{6}$3 ppb of G$_{1}$, and 11.39 ppb of G$_{2}$ with the total of 29.11 ppb was investigated. High performance liquid chromatograph (HPLC) was used for the separation and quantitation of aflatoxins. Treatment for 2 hours by the chemicals affected the destruction of aflatoxins and the most effective chemical was 10% sodium hypochlorite (p<0.05). Sodium hypochlorite concentrations more than 1% significantly reduced aflatoxin B$_{2}$, G$_{1}$, G$_{2}$ and total aflatoxins and more than 3% reduced B$_{1}$ (p<0.05). No further significant decreases were observed above the concentration of 5% for all 4 aflatoxins. Complete destruction of aflatoxins B$_{2}$, G_{1}$, and G$_{2}$ was achieved by 5% sodium hypochlorite at 48 hours and B$_{1}$ at 72 hours.

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Incidence and Level of Aflatoxins Contamination in Medicinal Plants in Korea

  • Lee, Sung Deuk;Yu, In Sil;Jung, Kweon;Kim, Yeon Sun
    • Mycobiology
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    • 제42권4호
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    • pp.339-345
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    • 2014
  • During 2011~2013, a total of 729 samples for 19 types of medicinal plant were collected from Seoulyekryungsi in Seoul, Korea, and investigated for the presence of aflatoxins. The samples were analyzed using immunoaffinity column cleanup and high-performance liquid chromatography coupled to a fluorescence detector after post-column derivatization. Aflatoxins were found in 124 out of the 729 analyzed samples: 65 containing aflatoxin B1 (AFB1), 24 with aflatoxin B2 (AFB2), 15 with aflatoxin G1 (AFG1), and 20 samples with aflatoxin G2 (AFG2). The ranges for positive samples were $0.1{\sim}404.7{\mu}g/kg$ for AFB1, $0.1{\sim}10.0{\mu}g/kg$ for AFB2, $0.1{\sim}635.3{\mu}g/kg$ for AFG1, $0.1{\sim}182.5{\mu}g/kg$ for AFG2, and $0.1{\sim}1,043.9{\mu}g/kg$ for total aflatoxins. Most of the medicinal plant samples (721, 98.9%) were below legal limits, but 8 samples exceeded the legal limits of 10 and $15{\mu}g/kg$ established by the Korean standard for AFB1 and total aflatoxins (the sum of AFB1, AFB2, AFG1 and AFG2), respectively.

진균류(眞菌類)의 상호작용(相互作用)에 의한 Aflatoxin 생성능(生成能)에 관(關)한 연구(硏究) (Effect of Various Fungi on the Aflatoxin Productivity in tile Culture of Asp. Flavus)

  • 김운주;정용;권숙표
    • Journal of Preventive Medicine and Public Health
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    • 제9권1호
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    • pp.77-86
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    • 1976
  • This study was attempted to know that the interactions of various fungi, and methionine and $MgSO_4$ introduced as the substrate of culture media for fungi were affected to produce aflatoxins by Asp. flavus. 5 different fungi were isolated from the fermented soybean mash and were cultured in Chemically Defined medium (C. D. media) and soybean mash at $25^{\circ}C$ for 10 days. (1) It was confirmed that Asp. flavus produced aflatoxins in the C. D. medium and soybean mast, but that Asp. niger, Asp. oryzae, Asp. awamori and Asp. terreus did not produced them respectively. (2) Asp. flavus cultured with Asp. niger did not produce aflatoxins in C. D. medium, but produced in soybean mash, in other hand, Asp. flavus with other fungi except Asp. niger produced aflatoxins in C. D. medium and soybean mash. (3) The growth of fungi were more prosperous in the seperate culture than in the mixed culture. (4) In the C. D. medium added 20% of cultured medium of Asp. niger, Asp. flavus did not produce aflatoxins but other cultured medium did not prohibit the production of aflatoxins by Asp. flavus. (5) On the contrary, $MgSO_4$ increasing the productivity of aflatoxins by Asp. flavus in the C. D. medium, methionine known as one of precursor of aflatoxins did not affected the increasing productivity with significance.

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한국발효식품중(韓國醱酵食品中) Aflatoxin의 함유(含有)에 관(關)한 연구(硏究) (Study on Aflatoxins in Korean Fermented Foodstuffs)

  • 정용;권숙표
    • Journal of Preventive Medicine and Public Health
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    • 제2권1호
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    • pp.1-4
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    • 1969
  • 35 samples of Korean fermented foodstuffs were tested to isolate and to identify for aflatoxins. Aflatoxin $G_1$ was detected in samples of soybean and Kanjang (Soybean sauce), and aflatoxins $G_1$ & $G_2$ in Meju (fermented soybean mass) and Dwenjang (fermented soybean paste). In the culture media of Aspergillus flavus aflatoxins $B_1,\;B_2,\;G_1\;and\;G_2$ were also isolated and identified. Aflatoxins were confirmed by the thin layer chromatography with methanol : chroroform (5:95v/v) developer and the ultra violet absorption spectrum.

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Relative toxicites of aflatoxins for the risk assessment

  • Kim, Jae-Hyun;Kim, Tae-Myoung;Lee, Sook-Jin;Park, Cheol-Beom;Hong, Jin-Tae;Yoo, Hwan-Soo;Kang, Jong-Koo;Kim, Dae-Joong;Yun, Young-Won
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2003년도 춘계학술대회 논문집
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    • pp.58-58
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    • 2003
  • Aflatoxins are metabolites of fungi Aspergillus spp. that world-widely contaminate diverse foodstuffs including com and peanuts. It is well known that aflatoxins are highly mutagenic, carcinogenic and possibly teratogenic. Although aflatoxins have received the great attention among the various mycotoxins due to their potent hepatocarcinogenicity in certain species, it is extremely crucial to elucidate the relative toxicity and carcinogenicity of the types (B$_1$, B$_2$, G$_1$ and G$_2$) of aflatoxins for the risk assessment.(omitted)

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식품중 Aflatoxin 측정방법의 비교 (Comparison of methods for Determination of Aflatoxins in food Products)

  • 김면희
    • 한국식품위생안전성학회지
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    • 제11권2호
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    • pp.149-157
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    • 1996
  • A procedure for the determination of Aflatoxins in food and grains which utilizes reversed phased liquid chromatographic (LC) analysis with postcolumn derivatization by an electrochemical cell and determination with a fluorescence detector has been evaluated. The LC mobile phase was water-acetonitrile-methanol (6+2+2) with 1mM KBr and 1 mM HNO3 which gave baseline separation for the four Aflatoxins (AfB1, AfB2, AfG1, AfG2). The electrochemical cell set at 7V, generated bromine and derivatized aflatoxins B1 and G1, The derivatives were detected by the fluorescence detector. The aflatoxins in naturally contaminated corn samples were isolated by three different cleanup procedures: the AOAC method I column(CB method), a rapid filtrate column (Romer's column), and an immunoaffinity column. The final extract were quantitated with fluordensitometric TLC and the LC postcolumn derivatization techniques. The results were quite similar, however the LC technique showed less interferences and could be automated. Samples of corn, raw peanuts, peanut butter and dried dates were also analyzed successfully with this procedure.

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Aflatoxin 분석법에 관한연구;추출 및 정제방법의 비교 (Comparative Study on the HPLC Determination of Aflatoxins Coupled with Extraction and Clean-up Methods)

  • 김종규
    • 한국식품위생안전성학회지
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    • 제8권4호
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    • pp.251-254
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    • 1993
  • Extraction and Clean-up procedures coupled with quantitation by high performance liquid chromatography(HPLC) was evaluated for the detection of 4 aflatoxins, B1, B2, G1 and G2, in peanut butter. The Sep-pak clean-up method showed poorer separation and repeatability than did the modified DeVries' and an immunoaffinity column clean-up methods. No significant difference of detected aflatoxins between the affinity column clean-up and modified DeVries' method. The coefficients of variation for the 4 aflatoxins were ranging from 6.3∼32.3 by the modified DeVries' method and 5.3∼9.8 by the affinity column clean-up.

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자가탁주와 땅콩버터에 대한 Aflatoxins 오염도의 검색 (The Detection of Aflatoxin in Home-made Takju and Peanut butter)

  • 오유진;윤여표;여신구;홍진태
    • 한국식품위생안전성학회지
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    • 제1권2호
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    • pp.171-176
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    • 1986
  • 충청북도 청원군 9개면에서 수집한 자가탁주 시료와 청주지역 시장에서 수집한 국내산 땅콩버터중의 aflatoxins 검색 결과 다음의 결론을 얻었다. 1. 자가탁주 시료에서는 78% 이상의 지역 시료에서 aflatoxin B1이 검출되었으며 평균 0.36 ppb, 최고 1.2 ppb까지 검출되었다. 2. 국내산 땅콩버터 시료에서는 aflatoxin류가 검출되지 않았다. 3. Sep-Pak silica cartridge에 의한 정제법은 기존의 column chromatography 정제법보다 회수율, 시간, 시약 등에서 훨씬 더 효율적이었다.

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