• Mycobiology
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• 제30권4호
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• pp.244-246
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• 2002

A destructive stem rot of tomato(Lycopersicon esculentum) occurred sporadically some farmers' fields in Jinju City, Gyeongnam province in Korea. The infected plants also showed stem, crown rot or whole plant blight. White mycelium spread over stems of infected plants and formed sclerotia on the old lesions nearby soil surface. The fungus showed maximum mycelial growth around $30^{\circ}C$. The fungus formed white colony on PDA, usually with many narrow mycelial strands in the aerial mycelium and the width were $4.0{\sim}9.8{\mu}m$. The typical clamp connections were formed on the mycelium. Numerous sclerotia was formed on PDA at $30^{\circ}C$. The shape of sclerotia was globoid and $1.0{\sim}3.0$ mm in size. The fungus was isolated repeatedly from the infected tissues and the pathogenicity was confirmed to tomato and identified as Sclerotium rolfsii. This is the first report on the stem rot of tomato caused by S. rolfsii in Korea.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.227.1-227
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• 1999

No Abstract, See Full Text

• Mycobiology
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• 제32권2호
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• pp.95-97
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• 2004

In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was $30^{\circ}C$ on PDA. The mycelial width ranged $4.2{\sim}10.4{\mu}m$ and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and $1.0{\sim}3.2$ mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.

• The Plant Pathology Journal
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• 제20권2호
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• pp.103-105
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• 2004

A destructive stem rot of strawberry (Fragaria x ananassa cv. Akihime) sporadically occurred in farmers' fields in Daegok-myon, Jinju city, Gyeongnam province in Korea. The infected plants showed stem and crown rot, with occasional blighting of the whole plant. White mycelia appeared on stems of infected clones and sclerotia formed on the old lesions near soil surface. The fungus formed white colony on PDA and showed maximum mycelial growth and sclerotial formation at $30^{\circ}C$. The fungus usually have many narrow hyphal strands, 2.6-10.0 $\mu\textrm{m}$ in width, in the aerial mycelium. Typical clamp connections were formed on the mycelium. Sclerotia were spherical and 1.0-2.4 mm in size. The fungus was repeatedly isolated from infected tissues and identified as Sclerotium rolfsii. Its patho-genicity was confirmed when inoculated onto straw-berry. This is the first report on the stem rot of strawberry caused by S. rolfsii in Korea.

• 한국응용곤충학회지
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• 제4권
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• pp.19-24
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• 1965

Tomato 즙배지에서 도열병균의 분생포자형성 및 균사의 생장에 환경요인이 주는 영향을 연구하고자 우선 광선조사조건(광원, 조사광선의 색, 조사시간), 전배양기간, 배지의 산도 등에 관하여 조사함으로써 간편한 방법으로 단시일내에 병원균의 분생포자를 다량 생성시킬 수 있는 방법을 모색코자 본실험을 하였다. (1) 24시간 계속 형광등에 조사시킨 것이 암처리한 것이나, 주기적으로 조사한 것보다 분생포자생장 및 균사생장을 증가시켰다. (2) 무피복, 적색, 황색, 청색의 Cellophane을 투과시킨 형광등조사에서 무피복이 가장 분생포자형성이 많았고 적색 및 황색, 청색 순으로 감소하였으며 균사생장에는 유의차가 없었다. (3) 도열병균도 광선의 주기적인 조사에 의하여 광선의 색에 관계없이 수상생장을 나타냈다. (4) 전배양기간이 길수록 광선조사에 의하여 분생포자의 형성은 증가되었지만 48시간에서 가장 좋았다. (형광등구). (5) 균총의 착색정도와 공중균사의 발달정도는 분생포자형성과 밀접한 관계가 있는 것 같으며 암색일수록 분생포자의 생장은 많으며 공중균사가 많으면 분생포자생성은 감소하는 경향이 있었다. 24시간 계속조사시킨 것이 가장 암색을 나타냈고 주기적인 광선조사는 중간정도였다. (6) pH 5-9에서 분생포자 및 균사생장을 볼 수 있었는데 그 최적은 pH 7이었으며 pH4 이하의 산도에서는 전연 병균의 생장을 볼 수 없었다.

• Journal of Microbiology
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• 제33권4호
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• pp.307-314
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• 1995

Trypsin like protease (TLP) and metalloprotease (MTP) were induced in associated with the mycelium differentiation in Streptomyces albidoflavus SMF301. TLP and MTP were purified and characterized from the culture. The molecular mass of TLP and MTP were estimated to be 32 kDa and 18 kDa, respectively. The molecular mass of TLP and MTP were estimated to be 32 kDa and 18 kDa, respectively. The optimum pH and temperature of TLP were 10 and 40.$^{\circ}C$ Those of MTP were 8 and 55 $^{\circ}C$ TLP was stable at alkaline pH (6-9) and unstable above 45.$^{\circ}C$and MTP was stable at alkaline pH and unstable above 80.$^{\circ}C$ Km and Vmax values with benzoyl-arginyl p-nitroanilide of TLP were 139 $\mu$M, and 10 nmole of nitroanilide released per min per$\mu\textrm{g}$ protein, respectively. Km, and Vmax values with a synthetic substrate, leucine p-nitroanilide, or MTP were 58.9 $\mu$M, 3.47 nmol of nitroanilide released per min per$\mu\textrm{g}$protein, respectively. TLP was inhibited competitively by leupeptin; the inhibition constant was 0.0031 $\mu$M. MTP was inhibited by EDTA, phenonthroline and bestatin.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.354-359
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• 1994

An isolate, 90-GT-302, was found to produce antibiotics inducing typical mycelial swelling in Magnaporthe grisea and Fusarium solani. This isolate formed yellow substrate and white rectiflexbiles aerial mycelia in the early stages of growth. The aerial mycelium gradually changed its color to white and finally formed a gray spore mass. Analysis of the cell wall acid hydrolysate revealed the presence of LL- and meso-diaminopimelic acids, glycine, and galactose, which indicated cell wall type X. This result placed our isolate in genus Kitasatosporia. A comparison of isolate 9O-GT-302 with reference strains of Kitasatosporia spp., which not only demonstrated several differences in their physiological properties but also novelty of the active compounds produced by this isolate, led us to designate the isolate as Kitasatosporia kimorexae.

• The Plant Pathology Journal
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• 제20권3호
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• pp.165-171
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• 2004

A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7％ and 95.1％, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.

• 한국버섯학회지
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• 제15권4호
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• pp.168-177
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• 2017

To evaluate effects of ligninolytic enzyme type on the mycelial response and ligninolytic enzyme production during interspecific interactions among wood-rotting fungi, 4 fungal strains, Trichophyton rubrum LKY-7, Trichophyton rubrum LSK-27, Pycnoporus cinnabarinus, and Trichoderma viride, were selected. Regarding ligninolytic enzyme production, LKY-7 secreted laccase and manganese peroxidase (MnP), P. cinnabarinus secreted only laccase, and LSK-27 secreted only MnP in glucose-peptone medium, while T. viride did not produce any ligninolytic enzymes. In the co-culture of LKY-7 with P. cinnabarinus, the formation of aerial mycelium was observed and the enhancement of laccase activity owing to interspecific interaction appeared to be very low. In the co-culture of LKY-7 and P. cinnabarinus with LSK-27, a hypha-free clear zone was observed, which resulted in deadlock, and increased laccase or MnP activity was detected at the interaction zone. The interaction responses of LKY-7, P. cinnabarinus, and LSK-27 with T. viride were characterized by the formation of mycelial barrages along the interface. As mycelial barrages were observed at the T. viride territory and no brownish pigment was observed in the mycelial barrages, it is suggested that laccase and MnP are released as part of an offensive response, not as a defensive response. The co-culture of P. cinnabarinus with T. viride lead to the highest enhancement in laccase activity, yielding more than 14-fold increase in laccase activity with respect to the mono-culture of P. cinnabarinus. MnP activities secreted by LKY-7 or LSK-27 was generally low in interspecific interactions.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.129.1-129
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• 2003

A destructive stem rot of strawberry (cv. Akihime) occurred sporadically in farmers' fields around Daegok-Myeon, Jinju City, Gyeongnam province in Korea. The infected plants showed stem and crown rot, sometimes whole plant blighted. White mycelia spread over stems of infected clones and sclerotia formed on the old lesions near to soil surface. The fungus formed white colony on PDA and showed maximum mycelial growth and scleotial formation around 30$^{\circ}C$. The fungus usually have many narrow mycelial strands in the aerial mycelium and the width were 4.0∼10.0$\mu\textrm{m}$. The typical clamp connections were formed on the mycelium. The shape of sclerotia was globoid and 1.0∼2.8 mm in size. The fungus was isolated repeatedly from the infected tissues and identified as Sclerotium rolfsii. The fungus was inoculated to strawberry and confirmed its pathogenecity This is the first report on the stem rot of strawberry caused by Scierotium rolfsii in Korea.