• Title/Summary/Keyword: Aeration speed

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Optimization of Culture Conditions and Continuous Production of Chitosan by the Fungi, Absidia coerulea

  • Kim, Woo-Jun;Lee, Woo-Gi;Kalaimahan Theodore;Chang, Ho-Nam
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.1
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    • pp.6-10
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    • 2001
  • The production of chitosan from the mycelia of Absidia coerulea was studied to improve cell growth and chitosan productivity. Culture conditions were optimized in batch cultivation (pH 4.5, agitator speed of 250 rpm, and aeration rate of 2 vvm) and the maximum chitosan concentration achieved was 2.3g/L under optimized conditions. Continuous culture was carried out successfully by the formation of new growth spots under optimized conditions, with a chitosan productivity of 0.052g/L(sup)-1 h(sup)-1, which is the highest value to date, and was obtained at a dulution rate of 0.05h(sup)-1. Cell chitosan concentrations reached about 14% in the steady state, which is similar to that achieved in batch culture. This study shows that for the continuous culture of Absidia coerulea it is vital to control the medium composition.

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Opitimization of culture conditions involved in dissolved oxygen for production of pullulan by Aureosidium pullulan

  • Seo, Hyeong-Pil;Son, Chang-U;Lee, Yu-Jeong;Kim, Seong-Gu;Kaplan, David L.;Lee, Jin-U
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.173-176
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    • 2002
  • Effect of carbon source and culture conditions involved in the concentration of dissolved oxygen on cell growth and the production of pullulan by A. pullulans HP2001 were investigated. Among those carbon sources, glucose was found to be the best carbon source for the production of pullulan by A. pullulans HP2001. Maximal production of pullulan by A. pullulans HP2001 was 26.6 g/ f when concentrations of glucose and yeast extract were 8% (w/v) and 0.25% (w/v), respectively. It was found that aeration rate, agitation speed and inner pressure of a bioreactor, which were some of physiological factors involved in the dissolved oxygen in the medium may affect cell growth and the production of pullulan by A. pullulans HP2001.

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Effects of Morphology and Rheology on Neo-fructosyltransferase Production by Penicillium citrinum

  • Lim, Jung-Soo;Lee, Jong-Ho;Kim, Jung-Mo;Park, Seung-Won;Kim, Seung-Wook
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.100-104
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    • 2006
  • In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (neo-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5vvm were 8.14 g/L and $53.2{\times}10^{-3} U/mL$, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

A Study, on the Minimum Oil Film Thickness of Connecting-rod Bearing in Engine (엔진 연결봉 베어링의 최소 유막 두께에 관한 연구)

  • Choi, Jae-Kwon;Hur, Kon;Han, Dong-Chul
    • Tribology and Lubricants
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    • v.10 no.1
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    • pp.14-26
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    • 1994
  • The minimum oil film thickness(MOFT) in the connecting-rod bearing of a 1.5 liter, L-4, gasoline engine is measured up to 5500 rpm and calculated to study the dynamically loaded engine bearing. Short bearing approximation and Mobility method are used for theoretical analysis of oil film characteristics. And cylinder pressure, crank-pin surface temperature and bearing temperature are measured as the input data of theoretical analysis. The MOFT are measured by the total capacitance method(TCM). To improve the reliability of the test results, a reasonable determination method of bearing clearance is introduced and used, and the effects of cavitation and aeration on the test results are neglected. The crankshaft is grounded by means of a slip ring. A scissor type linkage system was developed to measure the MOFT and bearing temperature. The effects of engine speed, load and oil viscosity on the measured and calculated minimum oil film thicknesses are investigated at 1500 to 5500 rpm. From the comparison between the measured and calculated MOFT, it is found that a qualitative similarity exists between them, but in all cases, the measured MOFT are smaller than those calculated.

Optimization of $\beta$-Galactosidase Production in Stirred Tank Bioreactor Using Kluyveromyces lactis NRRL Y-8279

  • Dagbagh, Seval;Goksungur, Yekta
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1342-1350
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    • 2009
  • This paper investigates the production and optimization of $\beta$-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in stirred tank bioreactor. Response surface methodology was used to investigate the effects of fermentation parameters on $\beta$-galactosidase enzyme production. Maximum specific enzyme activity of 4,622.7 U/g was obtained at the optimum levels of process variables (aeration rate 2.21 vvm, agitation speed 173.4 rpm, initial sugar concentration 33.8 g/L, incubation time 24.0 hr). The optimum temperature and pH of the $\beta$-galactosidase enzyme produced under optimized conditions were $37^{\circ}C$ and pH 7.0, respectively. The enzyme was stable over a pH range of 6.0-7.5 and a temperature range of $25-37^{\circ}C$. The $K_m$ and $V_{max}$ values for O-nitrophenol-$\beta$-D-galactopyranoside (ONPG) were 1.20 mM and $1,000\;{\mu}mol/min{\cdot}mg$ protein, respectively. The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in $\beta$-galactosidase enzyme production. Hence, this study fulfills the lack of using mathematical and statistical techniques in optimizing the $\beta$-galactosidase enzyme production in stirred tank bioreactor.

The Fermentation Characteristics of Newly Selected Thermotolerant Yeasts at High Temperature

  • Sohn, Ho-Yong;Park, Wan;Jin, Ing-Nyol;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.222-229
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    • 1994
  • In order to develop a method of economical production and to reduce energy-consumption in fuel alcohol production, we investigated the fermentation characters of two newly selected thermotolerant yeasts. The RA-74-2 showed stable and superior fermentability between 30 and $40^{\circ}C$ in 20% glucose media in comparison to the industrial strains. The optimum concentration of glucose for economical fermentation at $40^{\circ}C$ was 15-18%, and organic nitrogen was necessary for a satisfactory fermentation. The optimum pH was 4.0 and aeration was adversed for high temperature fermentation. Agitation was an important factor at $40^{\circ}C$ and the addition of magnesium ion 0.2% was required in this experiment. When the inoculum was increased, ethanol productivity as well as the speed of fermentation increased. On the other hand RA-912, which can grow at $48^{\circ}C$, showed similar fermentability between 30-$45^{\circ}C$ in 20% glucose media As the concentration of substrate decreased, fermentation ratio increased at $45^{\circ}C$ (45%, 65%, 95% fermentation ratio in 20%, 15%, 10% glucose media, respectively). Also, requirement of organic nitrogen and magnesium ion in RA-912 was similar in RA-74-2. The optimum pH for fermentation was 5.0, and the effects of agitation were enhanced at $37^{\circ}C$ than at $45^{\circ}C$. As the inoculum was increased, fermentation speed became more enhanced but the ethanol productivity was less affected. RA-912 showed fermentability with various substrates. Among the substrates used, inulin was the most promising substrate for the high-temperature fermentation. When 14.5% inulin was used as the substrate, 93% and 55% fermentation ratios were shown at $37^{\circ}C$ and $45^{\circ}C$, respectively.

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Conditions for Soluble Phosphate Production by Environment-Friendly Biofertilizer Resources, Pseudomonas fluorescens (환경친화적 미생물비료 자원 Pseudomonas fluorescens RAF15에 의한 가용성 인산 생산에 영향을 미치는 조건)

  • Park, Ki-Hyun;Park, Geun-Tae;Kim, Sung-Man;Lee, Chung-Yeol;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.17 no.9
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    • pp.1033-1037
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    • 2008
  • The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of $MgCl_2{\cdot}6H_2O$ and $MgSO_4{\cdot}7H_2O$ in the medium. Supplementation of medium with 0.01% $CaCl_2{\cdot}2H_2O$ and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% $MgCl_2{\cdot}6H_2O$, 0.01% $MgSO_4{\cdot}7H_2O$, 0.01% $CaCl_2{\cdot}2H_2O$ and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.

Study on Sludge Reduction by Sludge Solubilization and Change of Operation Conditions of Sewage Treatment Process (하수슬러지 가용화와 하수처리 운전조건 개선을 통한 하수슬러지 발생저감 연구)

  • Choi, In-Su;Jung, Hoe-Suk;Han, Ihn-Sup
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.12
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    • pp.1113-1122
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    • 2009
  • In order to find the way to solve the problem of sewage sludge discharge into the ocean, the sludge solubilization by ultrasonic and the improvement methods of wastewater treatment process were studied. In the membrane bioreactor the sludge retention time was stepwise increased from 5.1 day to 442 days where the biomass average concentration has been increased from $c_B$=3.4 $gTSSL^{-1}$ to $c_B$=14.5 $gTSSL^{-1}$ respectively. At the same time, the biomass yield coefficients were reduced from 0.5-0.7 at SRT=5.1 day to 0.005-0.007 at SRT=442 days which means the reduction of sludge production. Oxygen mass transfer coefficients and ${\alpha}$-factor were investigated with changing stirrer speed to find the relation between the high biomass concentration and aeration efficiency in the propeller loop reactor. As a result of sludge solubilization, the solubilization of sludge by ultrasound was increased with increasing energy input and it led to improved anaerobic digestion rate with more biogas production than that of nonsolubilized sewage sludge.

Fed-Batch Culture for Polyhydroxyalkanoate Overproduction by Pseudomonas sp. HJ (Pseudomonas sp. HJ로부터 Polyhydroxyalkanoate 대량생산을 위한 유가식 배양)

  • 손홍주;이상준
    • KSBB Journal
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    • v.11 no.2
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    • pp.201-210
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    • 1996
  • The production of polyhydroxyalkanoate(PHA) from glucose by batch and fed-batch culture of Pseudomonas sp. HJ was studied. In batch culture using fermentor, 400 rpm of agitalion speed, 2 vvm of aeration rate, 18 hours of inoculum age, and 5% (vlv) of inoculum size were optimal. PHA production was not increased by deficiency of oxygen. In a batch culture, the final call mass was $6.251g/\ell$, and PHA content was 20% of dry cell weight. In a constant feeding fed-batch culture, cell mass increased to $33.24g/\ell$, and PHA content reached 48.9% of dry cell weight. In an intermittent feeding fed-batch culture, cell mass increased to $37.89g/\ell$, and PHA content reached 53.5% of dty cell weight.

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Rhamnolipid Production in Batch and Fed-batch Fermentation Using Pseudomonas aeruginosa BYK-2 KCTC 18012P

  • Lee, Kyung-Mi;Hwang, Sun-Hee;Ha, Soon-Duck;Jang, Jae-Hyuk;Lim, Dong-Jung;Kong, Jai-Yul
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.4
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    • pp.267-273
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    • 2004
  • The optimization of culture conditions for the bacterium Pseudomonas aeruginosa BYK-2 KCTC 18012P, was performed to increase its rhamnolipid production. The optimum level for carbon, nitrogen sources, temperature and pH, for rhamnolipid production in a flask, were identified as 25 g/L fish oil, 0.01% (w/v) urea, 25 and pH 7.0, respectively. Optimum conditions for batch culture, using a 7-L jar fermentor, were 200 rpm of agitation speed and a 2.0 L/min aeration rate. Under the optimum conditions, on fish oil for 216 h, the final cell and rhamnolipid concentrations were 5.3 g/L and 17.0 g/L respectively. Fed-batch fermentation, with different feeding conditions, was carried out in order to increase, cell growth and rhamnolipid production by the Pseudomonas aeruginosa, BYK-2 KCTC 18012P. When 2.5 g of fish oil and 100 mL basal salts medium, containing 0.01 % (w/v) urea, were fed intermittently during the fermentation, the final cell and rhamnolipid concentrations at 264 h, were 6.1 and 22.7 g/L respectively. The fed-batch culture resulted in a 1.2-fold increase in the dry cell mass and a 1.3-fold increase in rhamnolipid production, compared to the production of the batch culture. The rhamnolipid production-substrate conversion factor (0.75 g/g) was higher than that of the batch culture (0.68 g/g).