Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Conditions for Soluble Phosphate Production by Environment-Friendly Biofertilizer Resources, Pseudomonas fluorescens

환경친화적 미생물비료 자원 Pseudomonas fluorescens RAF15에 의한 가용성 인산 생산에 영향을 미치는 조건

  • Park, Ki-Hyun (School of Applied Life Science, Pusan National University) ;
  • Park, Geun-Tae (Research & University-Industry Cooperation, Pusan National University) ;
  • Kim, Sung-Man (School of Bioresource Science, Pusan National University) ;
  • Lee, Chung-Yeol (School of Bioresource Science, Pusan National University) ;
  • Son, Hong-Joo (School of Applied Life Science, Pusan National University)
  • 박기현 (부산대학교 생명응용과학부) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 김성만 (부산대학교 생명자원과학부) ;
  • 이충열 (부산대학교 생명자원과학부) ;
  • 손홍주 (부산대학교 생명응용과학부)
  • Published : 2008.09.30
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Abstract

The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of $MgCl_2{\cdot}6H_2O$ and $MgSO_4{\cdot}7H_2O$ in the medium. Supplementation of medium with 0.01% $CaCl_2{\cdot}2H_2O$ and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% $MgCl_2{\cdot}6H_2O$, 0.01% $MgSO_4{\cdot}7H_2O$, 0.01% $CaCl_2{\cdot}2H_2O$ and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.

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References

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