• Journal of Industrial Technology
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• v.24 no.B
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• pp.171-176
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• 2004

The effects of agitation and aeration for exopolysaccharide(EPS) production through batch cultivation of an Enterobacter sp. isolated from the composter were investigated. During the EPS fermentation under conditions of constant agitation speed from 200 to 900 rpm and constant aeration rate of 0.5-2.5 vvm, the low yields of EPS(4.8-5.2g/L) was observed as the viscosity increase of culture broth. With the stepwise increases in agitation speed and aeration rate, the EPS production and the viscosity of EPS were increased 1.3~1.4 times and 2.3~3.6 times higher than those of the fixed conditions, respectively. Therefore, these stepwise increases were considered as the key operating parameters for enhancing EPS production. The max. EPS(6.8g/L) and viscosity(14,000cp) were obtained when the agitation speed was increased from 300 to 900 rpm for 54hrs at 1.5 vvm.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.192-195
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• 2002

Effect of aeration rate and agitation speed on cell growth and the production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica was investigated. Aeration rate and agitation speed in a 7L bioreactor ranged from 0.5 to 1.5 vvm and from 300 to 500 rpm, respectively. Higher agitation speed with an aeration rate of 0.5 vvm in the bioreactor resulted in maintenance of higher concentration of dissolved oxygen in the medium, which enhanced the production of PS-7. In this study with a 7L bioreactor, maximal production of PS-7 was 11.0 g/L and its conversion rate from 2% (w/v) glucose was 0.55 when the aeration rate and agitation speed were 1.0 vvm and 500 rpm, respectively. Proper aeration rate and agitation speed might enhance the production of PS-7 as well as reduce the time to reach maximal production.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2001.06a
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• pp.201-211
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• 2001

The influence of aerated oil on high-speed journal bearing is examined by classical thermohydrodynamic lubrication theory coupled with analytical models for viscosity and density of aerated oil in fluid-film bearing. Convection to the walls, mixing with supply oil and re-circulating oil, and some degree of journal misalignment are considered. The considered Parameters for the study of bubbly lubrication are oil aeration level, air bubble size, shaft misalignment and shaft speed. The results show that deliberate oil aeration can more clearly bring on the increasing load capacity under high-speed operation of plain journal hearing than previous normal speed operation. And the load capacity may be increased more by oil aeration under the conditions of shaft misalignment and the increasing speed.

• KSBB Journal
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• v.16 no.3
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• pp.307-313
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• 2001

The effects of agitaion and aeration on mycelial growth, exo-polysaccharide (EPS) production, and substrate consumption upon the submerged cultivation of G. lucidum were investigated, and the batch kinetics of the EPS fermentation of G. lucidum were interpreted as function of agitation speed and aeration rate. In a 2.6 L jar fermenter system, the optimum agitation speed and aeration rate for EPS production were determined to be 400 rpm and 1.0 vvm, respectively. The maximum production of EPS obtained was 15 g/L. The logistic model for mycelial growth fitted the experimental data better than that determined by the Monod and the two-thirds power models. The Luedeking-Piret equation adequately modelled the kinetic data obtained for product and substrate.

• Tribology and Lubricants
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• v.17 no.4
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• pp.321-334
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• 2001

The influence of aerated oil on high-speed journal bearing Is examined by thermohydrodynamic lubrication theory to lubrication with mixtures of a Newtonian liquid and an ideal gas. For this purpose, analytical models for viscosity and density of aerated oil in fluid-film bearing are applied. Convection to the walls, mixing with supply oil and re-circulating oil, and some degree of journal misalignment are considered. The results show that deliberate oil aeration can increase the load capacity of high-speed plain Journal bearing. And the load capacity is increased more by oil aeration under the conditions of shaft misalignment and higher speed.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.7
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• pp.129-135
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• 1999

We developed a new measuring equipment which makes it possible to perform on-line measurements with the merits of its implicity and the time-saving characteristics. By using the newly developed measurement system, the amount of aeration is directly measured on several parameters, i.e, engine speed, oil quantity, oil deterioration, oil temperature and viscosity, etc. It showed that oil aeration is strongly related to be gas movements in crankcase and the residence time of circulating oil in oil pan. In addition, in order to clarify the mechanism of aeration and to quantify the degree of aeration, a modelling analysis to predict aeration was performed , and as a guiding parameter, Aeration Index was defined. Finally, the parameter was compared with the actual amount of aeration, and it was confirmed that they gave a good correlation with each other.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.343-346
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• 2001

The effect of agitation speed and aeration rate on mTG production and cell growth by Streptoverticillum morbarense was investigated. Dissolved oxygen was controlled by on-line computer-controlled fermentation system. The agitation speed and aeration rate of 2.5 L fermentor ranged from 330 to 360 게m and 1 vvm to 4 vvm, respectively. The highest mTG production was 2.1 U/mL when dissolved oxygen level was 20%, and it was improved almost 1.1 times in comparison with that without dissolved uxygen control.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.292-297
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• 1993

Aspergillus niger No. PFST-38 was grown on complex media in 30L agitated fermentors at various aeration rates and stirrer speeds. We could correlate the mixing time as a function of the Reynolds number and the apparent viscosity, as follows. ${\theta}_M=2.95\;\NRe^{-0.52},\;{\theta}_M=1.88\;{\eta_a}^{0.57}$ Also, the effects of the apparent viscosity (${\theta}_a$), the impeller rotational speed (N), the air flow rate ($V_s$), and the mixing time (${\theta}_M$) on the oxygen transfer coefficient, $K_L a$ were determined experimentally, and equated as follows. $K_La=12.04N^{0.88}Vs^{0.71}{n_a}^{-0.83},\;K_La=30.2N^{0.88}Vs^{0.71}{\theta_M}^{-1.45}$ $K_La$ increased as the agitation speed and the air flow rate increased. The rate of $K_La$ increase was dependent more on the rotational speed of impeller than on the air flow rate. The glucoamylase production increased with the increase of the agitation speed upto at 500 rpm and increased with the increase of air flow rate upto at 1.0 vvm. The values calculated from the above equation confirmed that the experimental maximum production of glucoamylase was achieved when the $K_La$ and the apparent viscosity of the broth were $260\;hr^{-1}$ and 1800 cps, respectively.

• Tribology and Lubricants
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• v.17 no.3
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• pp.198-208
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• 2001

A Parametric study based on a computer analysis of the lubrication system of a four-cylinder gasoline engine is illustrated. Through the parametric study, the effects of various aeration levels on the change of oil flow rate and pressure are investigated. Also, at high oil temperature and low engine speed, the effect of oil aeration level on oil flow characteristics in lubrication system is investigated. The illustrated results may give to designers the guide lines of oil aeration level for the safe design of engine lubrication systems in terms of minimum pressure at crank oil bore.

• Biotechnology and Bioprocess Engineering:BBE
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• v.1 no.1
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• pp.32-35
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• 1996

Amoving aeration-membrane (MAM) bioreactor was employed for the production of 2$\mu$g/mL of tissue type Plasminogen Activator (tPA)in serum free medium from normal human fibroblast cells. This system could maintain high cell density for long periods of steady state conditions in perfusion cultivation. Under normal operating condition, shear stress was as low as 0.65 dynes/$\textrm{cm}^2$ at the agitation speed of 80 rpm. Even though cell density gradually decreased with increasing agitation speed, tPA production increased linearly with increasing shear stress within a moderate range. This culture system allowed production of 2$\mu$g tPA/mL while maintaining a high cell denisty of 1.0$\times$107 viable cell/mL.