• Title/Summary/Keyword: Aedes

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Comparative Expression of the Aedes aegypti 5-Hydroxytryptamine7 Receptor in Drosophila Schneider2 and Chinese Hamster Ovary-K1 Cells (초파리 Schneider2 세포와 Chinese hamster ovary-K1 세포에서 Aedes aegypti 5-Hydroxytryptamine7 수용체의 발현비교)

  • 이대원
    • Korean journal of applied entomology
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    • v.43 no.2
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    • pp.155-162
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    • 2004
  • Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT$\_$7/ receptor of Aedes aegypti, the Aedes 5-HT$\_$7/ receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-Kl cells. The expression of the Aedes 5-HT$\_$7/ receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT$\_$7/ receptor is much more effective in mammalian CHO-K 1 cells and that the Tr-CHO cell line expressing Aedes 5-HT$\_$7/ receptor can be used for synthetic agonist or antagonist candidate screening.

CLIMEX-based Analysis of Potential Geographical Distribution of Aedes albopictus and Aedes aegypti in South Korea

  • Jung, Jae-Min;Lee, Ji-Won;Kim, Chang-ju;Jung, Sunghoon;Lee, Wang-Hee
    • Journal of Biosystems Engineering
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    • v.42 no.3
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    • pp.217-226
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    • 2017
  • Purpose: Aedes aegypti and Aedes albopictus are notorious disease vectors that spread various viruses including dengue, yellow fever, chikungunya, and Zika. Recent Zika virus outbreaks were carried by Ae. aegypti, raising awareness about the perils of its global distribution. Because Ae. albopictus is spread throughout South Korea and can carry the same viruses as Ae. aegypti, monitoring potential distributions of Ae. albopictus and Ae. aegypti is necessary. Methods: In this study, the potential distributions of Ae. albopictus and Ae. aegypti in South Korea were modeled using CLIMEX software, and changes in response to climate change were predicted. Results: The results indicated that the climatic suitability for Ae. albopictus was consistently high, while occurrence of Ae. aegypti was only predicted for Jeju Island in 2080. Conclusions: The results provide basic information for preventing the invasion of Ae. aegypti, and consequent interactions between Ae. aegypti and Ae. albopictus, which may cause severe outbreaks of dangerous diseases.

Taxonomy of the Genus Aedes (Insecta: Diptera: Culicidae) with the Microstructure of the Egg Surface (난표면 미세구조에 의한 숲모기속(곤충강: 파리목: 모기과)의 분류)

  • 윤종호;박희천
    • Animal Systematics, Evolution and Diversity
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    • v.12 no.3
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    • pp.237-244
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    • 1996
  • he observation of the egg microstrutures on the five Korean mosquito species showed some useful results to identify the species. There have appeared five classified groups with characters of egg structured on 20 Aedes species and 3 Culex specie니 in the cluster analysis. Among the 23 species, A desmotes and A. mediopunctatus perplexus were completely separated form other Aedes species.

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Repellent activity of aromatic medicinal plant extracts against Aedes aegypti(Diptera: Culicidae) (방향성 한방식물체 조추출물의 에짚트숲모기(Aedes aegypti)에 대한 기피활성에 관한 연구)

  • 최한영;양영철
    • Journal of environmental and Sanitary engineering
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    • v.17 no.3
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    • pp.110-114
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    • 2002
  • The repellent activity of methanol extracts from 8 aromatic medicinal plant species and a steam distillate against blood-starved Aedes aegypti (L.) females was laboratory examined by skin test and compared with that of N,N-diethyl-m-toluamide (deet). Reponeses varied according to Plant species. At a dose of $0.1mg/\textrm{cm}^2$, potent repellency against adult mosquitoes was obtained with extracts of Cinnamomum camphora (94.1 %) Cinnamomum cassia bark (91.2%), Eugenia caryophyllata flower bud (72.2%), and Tilia amurensis (69.4%). Repellent activity of these plant extracts was comparable to that of deet (83.4%). The plants described merit further study as potential mosquito-repellent agents.

Unrecorded Aedes (Edwardsaedes) bekkui (Diptera: Culicidae) from Korea: Notes on Taxonomic Diagnosis of Adult and Larva (한국산 모기과의 미기록종 베꾸숲모기(Aenes bekkui) : 성충 및 유충의 분류학적 특징에 관하여)

  • ;Rob
    • Parasites, Hosts and Diseases
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    • v.25 no.2
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    • pp.210-212
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    • 1987
  • 한국산 모기에 대한 조사연구사업의 일환으로 1387년 6월 경기도 남양주군 광릉에 위치한 임업시험장 일대에서 야외채집을 실시한 결과 미기록종 Aedes bekkui(베꾸숲모기)를 채집하였기에 이를 보고한다. 성충의 특징은 기문하구(subspiracular area)에 작을 강모가 없는 점이고, 유충의 특징은 머리털 4-C와 6-C의 간격이 좁고 5,6-C의 크기와 모양은 서로 대등하며 6,7-C의 간격은 5,6-C의 간격보다 2배이상 넓다는 점이다. 또 꼬리틸 3-X는 단모로 되어 있고 2-X보다 상당히 길다. Tanaka의 검색표에 따르면 Aedes bekkui 성충의 특징은 아속 Edwardsaedes와 일치되지만 유충은 아속 Aedimorphus에 일치된다. 이는 Belkin이 처음 아속 Edwardsaedes를 확립할 때 유일하게 Aedes imprimens를 단모식 표본종(monotypic species)으로 이용하여 그 특징 열거에 있어 너무 단조로 왔기 때문이며 또한 일본에서 A. imprimens가 A. bekkui로 판명되기까지 자충의 특징만이 기록되어 왔기 때문이다. 그러므로 과거 한국에서 보고된 A. imprimens도 일본의 경우와 같이 A. bekkui의 오식종이었을 것으로 생각된다.

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Effect of Copper on Lethal Dose and Pupation of Aedes albopictus (Diptera: Culicidae) (흰줄숲모기, Aedes albopictus (Diptera: Culicidae)의 치사농도 및 용화에 대한 구리의 영향)

  • Lee, Su-Mi;Yoon, Su-Jin;Shin, Byung-Sik
    • Korean Journal of Environmental Biology
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    • v.28 no.2
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    • pp.64-68
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    • 2010
  • The mosquito, Aedes albopictus is important vector of humen phatogen such as dengue fever. And Aedes albopictus has widly distribution in oriental regions. This study was conducted to investigate the biological effects of cooper on the development of the Asian tiger mosquitoes, Aedes albopictus (Skuse). Aedes albopictus was affected with pupation ratios, mortalities, and wing lengths of the 3th and 4th instars by exposure to different concentration $CuCl_2$ (0.0, 2.5, 25.0 and 50.0 ppm) for 24 h and 48 h depending on dose and exposure period. The lowest $LC_{50}$ (median lethal concentration) value of $CuCl_2$ was 18.1 ppm at the 3rd instars for 48 h exposure. The pupation ratio of the larvae exposed at 50.0 ppm for 48 h was 14% which was much lower than those exposed to other treatment groups Changes of adult wing length showed significant decreased by Cu treatment. In conclusion, the most prominent effects of high concentration and longer exposure period were to reduce in the survival and pupation rates of larvae and wing length of Ae. albopictus.

Seasonal prevalence and behaviour of Aedes togoi (토고숲모기(Aecles togoi)의 계절적 발생소장 및 습성에 대하여)

  • 이종수;홍한기
    • Parasites, Hosts and Diseases
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    • v.33 no.1
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    • pp.19-26
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    • 1995
  • Ecological studies of Aedes togoi, the vector of malayan filariasis, were ci,wind out at Tolsando, Yosu and Sokcho area in 1991. The adult population of Aedes togoi was continuously appeared from the first week of April to the end of November showing the highest density in .truly. The larvae of Aedes togoi were found at rock pools from March to December in Sokcho area and the density was highest in July and August, whereas in the southern coastal area (Yosu), the larvae were found throughout the year and the density was the highest in Tune. The rate of larvae inhabited below 0.5% salinity was 45.7% in Sokcho and 51.7% in Yosu. The feeding activity of Aedes togoi was nocturnal, with the peak period of 01 :00-03:00 hours. Indoor feeding activities were slightly higher than outdoors showing the biting ratio of 1 :0.8 (indoor: outdoor). The average number of Aedes togoi attracted to CO2 gas was 8.5 whereas 117 Anophelei sinensis was attracted. The result indicates that CO2 is not an effective attractant for host seeking of Aedes togoi compared to Anopheles sineteis. The most common place was bedroom with 54.5% of total collections and next to stock place (18.2%), floor(9.1%) and kitchen (9.1%).

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The Impact of Urban Heat Island-induced Temperature Differences on the Hatching Rates of Aedes albopictus (도시열섬 현상에 의한 기온차이가 흰줄숲모기(Aedes albopictus) 부화율에 미치는 영향)

  • Jihun Ryu;Kwang Shik Choi
    • Korean journal of applied entomology
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    • v.63 no.1
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    • pp.77-80
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    • 2024
  • Aedes albopictus, a common species in the Republic of Korea, is internationally known as a major vector for various diseases, and it is well-adapted to urban environments. Recent insect outbreaks in urban areas, attributed to climate change and urban heat islands, have increased the necessity of researching the effects on mosquito populations. This study analyzed climate data from 25 Automatic Weather System (AWS) stations in Seoul, identifying urban areas with pronounced heat island effects and suburban areas with milder effects. Nine urban heat island conditions were established based on this analysis, under which the hatching rates of Ae. albopictus were examined. The results revealed an increase in hatching rates correlating with the intensity of the urban heat island effect. Regression analysis further indicated that this trend accelerates as the strength of the heat island effect increases. This study suggests that temperature variations resulting from urban heat island phenomena can significantly influence the hatching rates of Ae. albopictus.

Identification of Two Isoforms of Aminopeptidase N in Aedes aegypti Larval Midgut

  • Pootanakit, Kusol;Angsuthanasombat, Chanan;Panyim, Sakol
    • BMB Reports
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    • v.36 no.5
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    • pp.508-513
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    • 2003
  • The bacterium Bacillus thuringiensis produces toxin inclusions that are deleterious to target insect larvae. These toxins are believed to interact with a specific receptor protein(s) that is present on the gut epithelial cells of the larvae. In various insect species (in particular those belonging to the lepidopteran class), aminopeptidase N (APN) is one of the two receptor proteins that are considered to be involved in toxin-receptor interactions. However, in mosquitoes, the nature and identity of the receptor protein is unknown. Here, using RT-PCR, we identified two isoforms of the APN transcripts in the Aedes aegypti mosquito larval midgut. These results are congruent with a previous report of multiple isoforms of the APN gene expression in lepidopteran larvae. Which of the two isoforms (or other yet unidentified receptor proteins) is involved in the killing of mosquito larvae remains to be elucidated.