• Nuclear Engineering and Technology
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• v.40 no.6
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• pp.467-476
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• 2008

To keep the superconducting (SC) magnet coils of KSTAR at proper operating conditions, not only the coils but also other cold components, such as thermal shields (TS), magnet structures, SC bus-lines (BL), and current leads (CL) must be maintained at their respective cryogenic temperatures. A helium refrigeration system (RRS) with an exergetic equivalent cooling power of 9 kW at 4.5 K without liquid nitrogen ($LN_2$) pre-cooling has been manufactured and installed. The main components of the KST AR helium refrigeration system (HRS) can be classified into the warm compression system (WCS) and the cryogenic devices according to the operating temperature levels. The process helium is compressed from 1 bar to 22 bar passing through the WCS and is supplied to cryogenic devices. The main components of cryogenic devices are consist of cold box (C/B) and distribution box (D/B). The C/B cool-down and make the various cryogenic helium for the KSTAR Tokamak and the various cryogenic helium is distributed by the D/B as per the KSTAR requirement. In this proceeding, we will present the commissioning results of the KSTAR HRS. Circuits which can simulate the thermal loads and pressure drops corresponding to the cooling channels of each cold component of KSTAR have been integrated into the helium distribution system of the HRS. Using those circuits, the performance and the capability of the HRS, to fulfill the mission of establishing the appropriate operating condition for the KSTAR SC magnet coils, have been successfully demonstrated.

• Proceedings of the Korean Information Science Society Conference
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• 2007.06b
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• pp.437-440
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• 2007

최근 환경 문제를 해결하기 위하여 환경 문제의 많은 컴퓨터 기술들이 응용되고 있다. 환경 정보는 환경오염원 및 주위에 미치는 영향과 오염된 농도에 대한 정보를 연구 및 분석 할 수 있는 분야이다. 최근 환경에 관련 연구가 일부에서 수행되고 있으나 이를 IT기술과 접목하여 다양한 정보를 효율적으로 저장 및 검색 할 수 있는 시스템은 부족한 실정이다. 또한 현재 환경오염의 측정방법으로는 수작업으로 이루어지는 경우가 많아서 공간적 및 물리적 제약이 있는 따르는 실정이다. 환경오염에 관한 정보를 센서를 사용하여 사용자에게 실시간으로 효과적으로 저장 관리하여 관련된 연구자들 간에 서로가 필요한 정보를 쉽게 공유할 수 있도록 본 논문에서는 Ubiquitous Sensor Network(USN), Java Message Service(JMS) 및 Webservice를 이용한 환경 모니터링 서비스를 제안 한다. 본 논문에서 제안한 시스템은 환경정보와 사용자간에 유연성, 확장성 및 실시간을 보장해 주는 시스템을 구현한다.

• International Journal of Aeronautical and Space Sciences
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• v.14 no.4
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• pp.356-368
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• 2013

The Korea Aerospace Research Institute plans to launch a lunar module by 2025, and so is carrying out a preliminary study. Landing stability on the lunar surface is a key design factor of a lunar module. In this paper, a 1/6 scale model of a lunar module is investigated, for its landing stability on non-level surfaces. The lunar module has four tripod legs, with aluminum honeycomb shock absorbers in each leg strut. ADAMS$^{TM}$, the most widely used multi-body dynamics and motion analysis software, is used to simulate the module's lunar landing. Three types of dampers in the struts (rigid, viscous, and aluminum honeycomb dampers), and two types of lunar surfaces (rigid and elastic) are considered. The Sforce function is adopted, to model the aluminum honeycomb dampers. Details on the modeling and analysis of the landing stability of the 1/6 scale lunar module and the simulation results are provided in this paper.

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1429-1433
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• 2006

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.959-965
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• 2013

Monascus species have been used to produce fermented rice called Monascus-fermented rice (MFR). To improve a Monascus strain via activation of secondary metabolite (SM) gene clusters for use in the production of MFR, we overexpressed an ortholog of the laeA gene, which encodes a global positive regulator of secondary metabolism under the control of the strong heterologous Aspergillus nidulans alcA promoter in Monascus pilosus. The OE::laeA transformant produced more SMs, including those not detected under uninduced conditions. MFR produced using the M. pilosus OE::laeA strain contained 4 times more monacolin K, a cholesterol-lowering agent, than MFR produced using the wild-type strain. In addition, pigment production was remarkably increased, and the antioxidant activity was increased as well. The results from this study suggest that Monascus species, which are important industrial fermentative fungi in Asia, can be improved for the production of functional foods by overexpressing the laeA gene.

• Bulletin of the Korean Chemical Society
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• v.30 no.11
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• pp.2723-2728
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• 2009

The conformational properties of oligosaccharides are important to understand carbohydrate-protein interactions. A trimannoside, methyl 3,6-di-O-($\alpha$-D-Man)-$\alpha$-D-Man (TRIMAN) is a basic unit of N-linked oligosaccharides. This TRIMAN moiety was further modified by GlcNAc (BISECT), which is important to biological activity of N-glycan. To characterize the trimannoside and its bisecting one we performed a molecular dynamics simulation in water. The resulting models show the conformational transition with two major and minor conformations. The major conformational transition results from the $\omega$ angle transition; another minor transition is due to the $\psi$ angle transition of $\alpha$ (1 $\rightarrow$ 6) linkage. The introduction of bisecting GlcNAc on TRIMAN made the different population of the major and minor conformations of the TRIMAN moiety. Omega ($\omega$) angle distribution is largely changed and the population of gt conformation is increased in BISECT oligosaccharide. The inter-residue hydrogen bonds and water bridges via bisecting GlcNAc residue make alterations on the local and overall conformation of TRIMAN moiety. These changes of conformational distribution for TRIMAN moiety can affect the overall conformation of N-glycan and the biological activity of glycoprotein.

• Smart Structures and Systems
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• v.10 no.6
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• pp.501-515
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• 2012

We design and test a shape memory alloy (SMA)-based actuation system that can be used to propel a fish robot. The actuator in the system is composed of a 0.1 mm diameter SMA wire, a 0.5 mm-thick glass/epoxy composite strip, and a fixture frame. The SMA wire is installed in a pre-bent composite strip that provides initial tension to the SMA wire. The actuator can produce a blocking force of about 200 gram force (gf) and displacement of 3.5 mm at the center of the glass/epoxy strip for an 8 V application. The bending motion of the actuator is converted into the tail-beat motion of a fish robot through a linkage system. The fish robot is evaluated by measuring the tail-beat angle, swimming speed, and thrust produced by the tail-beat motion. The tail-beat angle is about $20^{\circ}$, the maximum swimming speed is about 1.6 cm/s, and the measured average thrust is about 0.4 gf when the fish robot is operated at 0.9 Hz.

• Korean Journal of Metals and Materials
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• v.49 no.8
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• pp.649-656
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• 2011

The present study evaluated the microstructures and mechanical properties of severely deformed Ni-30Cr alloys. Cross-roll rolling (CRR) process was introduced as a severe plastic deformation (SPD), and Ni-30Cr alloy sheets were cold rolled to 90% thickness reduction and subsequently annealed at $700^{\circ}C$ for 30 min to obtain the recrystallized microstructure. Electron back-scattering diffraction (EBSD) was introduced to analyze grain boundary character distributions (GBCDs). The application of CRR to the Ni-30Cr alloy was effective in enhancing the grain refinement through heat treatment; consequently, the average grain size was significantly refined from $33{\mu}m$ in the initial material to $0.6{\mu}m$. This grain refinement directly improved the mechanical properties, in which yield and tensile strengths significantly increased relative to those of the initial material. We systematically discuss the grain refinement and accompanying improvement of the mechanical properties, in terms of the effective strain imposed by CRR relative to conventional rolling (CR).

• Proceedings of the Korea Information Processing Society Conference
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• 2009.11a
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• pp.381-382
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• 2009

본 논문은 고령자 및 장애인의 안전한 이동을 지원하기 위한 지능형 휠체어에서의 자동 장애물 감지 및 회피 기술을 개발한다. 이때 다양한 환경에서의 장애물을 정확히 감지하고 회피하기 위하여 학습을 이용한 비전 기반의 경로 선정 방법이 제안 된다. 제안된 시스템은 배경 분류기, occupancy grid map 생성기, 경로 선정기로 구성되며, 경로 선정 시 강건한 장애물 검출을 수행하기 위해 입력 영상을 occupancy grid map으로 변환하고, K-means clustering 알고리즘을 이용하여 생성된 대표 템플릿들과 비교하여 이동 가능한 방향을 선정한다. 제안된 시스템의 효율성을 증명하기 위해 다양한 형태의 장애물을 포함하는 실내 및 실외에서 실험한 결과 81.7%의 정확도를 보였으며, 지능형 휠체어 사용자에게 안전한 이동성을 제공 할 수 있음을 증명 하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.155-162
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• 2002

The cyclic amidohydrolase family enzymes, which include allantoinase, dihydroorotase, dihydropyrimidinase and (phenyl)hydantoinase, are metal-dependent hydrolases and play a crucial role in the metabolism of purine and pyrimidine in vivo. Each enzyme has been independently characterized, and thus well documented, but studies on the higher structural traits shared by members of this enzyme family are rare due to the lack of comparative study. Here, we report upon the expression in E. coli cells of maltose-binding protein (MBP)- and glutathione S-transferase (GST)-fused cyclic amidohydrolase family enzymes, facilitating also for both simple purification and high-level expression. Interestingly, the native quaternary structure of each enzyme was maintained even when fused with MBP and GST. We also found that in fusion proteins the favorable biochemical properties of family enzymes such as, their optimal pHs, specific activities and kinetic properties were conserved compared to the native enzymes. In addition, MBP-fused enzymes showed remarkable folding ability in-vitro. Our findings, therefore, suggest that a previously unrecognized trait of this family, namely the ability to functional fusion with some other protein but yet to retain innate properties, is conserved. We described here the structural and evolutionary implications of the properties in this family enzyme.