This study was conducted to evaluate the effect of the conventional pesticide application system on the rice spider community based on the quantitative investigation of arthropod community in the rice fields in Saran area, Hwasung-si, Kyeonggi-do from 1996 to 1998. Spiders were the highest dominant group comprising over 90% of all natural enemies in their density, and showed very stable yearly occurrence. In these fiekds the mean density of spiders was about 10 individuals/$0.25m^2$ during the rice growing season, and three families including Lycosidae, Tetragnathidae and Linyphiidae were very abundant. The colonization of spiders occurred in early season and they showed rapid density increase in mid-season. They maintained the highest density to the late season and their density decreased at harvest season. In the years of the low rice pest occurrence, the disturbance effect on spider community by pesticides were less. Two dominant species, Pirata subpiraticus and Pachygnatha clerki, had different population dynamics; Pirata subpiraticus showed the rapid density increase in the mid-July, and most of them remained immature stages, however, in the case of Pachygnaths clerki population, they rapidly increased in the late August and most of them remained adult stage during the late growing season.
This study used adult wistar-based rats to observe the sexual cycle as a morphological characteristic of vaginal epithelial cells by vaginal smearing, and investigated the fetal number through mating with male rats of the same strain. The target animal was a 12 to 13-week-old Wistar-based mature unlighted rat (weight 220 g to 240 g), room temperature 23 ± 2℃, 14 hours artificial lighting (05:00 to 19:00 hours), 10 hours Adapted individuals were used for rearing for at least 2 weeks under the conditions of the darkroom (19:00 to 05:00). The feed was managed for free feeding of pellet feed for animals and water. The vaginal smearing method was used for the experiments by observing the sexual cycle every morning and confirming that the normal sexual cycle of 4 or 5 days was repeated at least 2 cycles or more. As a result, the proestrus was found to have few red blood cells, the cells and nuclei were rather large and round, and many nucleated cells were identified. In the case of the estrus, the cells were large and the nuclei were not stained, and most of the keratinocytes were found. In addition, in the metestrus and diestrus, there were many white blood cells, and it was confirmed that nucleated epithelial cells and keratinocytes were significantly reduced. The pregnancy period was 21 ± 1.8 days, and the number of live births per delivery was 11.9 on average. The number of fetuses on the 8th and 10th days of pregnancy were 15.2 ± 0.4 and 15.4 ± 0.3, respectively. On the contrary, the number of fetuses on the 12th day of pregnancy was 12.9 ± 0.6, which was significantly (p < 0.05) decreased compared to the 10th day of pregnancy, and the number of fetuses was similar until delivery. As a result of investigating the change of body weight according to the birth weight and growth stage after delivery, the birth weight of female and male was 9.2 ± 2.0 g and 9.8 ± 2.5 g, respectively. After that, until the 16th day, the female and the male showed similarly moderate weight gain, and then showed a rapid weight gain until the 21st day of lactation. With reference to the results of this study, it is expected to be used as basic data for determining the mating time of rodents and controlling pregnancy and fetal number.
Adult rabbits were anesthetized with nembutal, 30 mg/kg. Carotid artery and jugular vein were exposed surgically and cannulated with polyethylene tubing. Arterial blood pressure was recorded via pressure transducer on the physiograph and $100{\mu}g/ml$ of histamine solution was infused through the jugular vein by using the constant infusion pump with a rate of 0.92 ml/min or 1.40 ml/min. Mean arterial blood pressure was maintained at $40{\sim}70 mmHg$ and hypotension was kept for 2 hours. After the termination of this period, blood was taken and osmotic fragility was mea sured immediately. Also, every sample of normal blood and shocked blood was incubated for 1 hour or 2 hours at $37^{\circ}C$ in order to see whether or not there was some influence of incubation. Furthermore to clarify which component was responsible for the change on the fragility, the mixtures of normal blood cells with shocked plasma and shocked blood cells with normal plasma were also incubated at $37^{\circ}C$ for one or two hours and fragility in such cases was measured. The data obtained were analysed by probit-plot method and the concentration of saline solution at which the hemolysis started to occur, 50% of blood cells were hemolysed and that at which the red blood cells hemolysed completely were determined. The values for the blood of hypotension stage were compared with those of the control blood. The results obtained were as fellows: 1. Osmotic fragility of red blood cell was increased in hypotensive state induced by histamine. 2. The differences of osmotic fragility after two hours of incubation were negligible both in normal blood and in that of hypotensive state. 3. Osmotic resistance of normal red blood cell incubated in shock plasma was less than that of shock red blood cell incubated in normal plasma. It was suggested that plasma in hypotensive state caused by histamine might be primarily responsible for the alteration of red blood cell fragility.
Total parenteral nutrition (TPN) is necessary to neonates in neonatal intensive care unit (NICU) for survival and growth because of impossible of enteral feeding. Long-term TPN can be associated with a broad spectrum of hepatobiliary disorder, ranging from mild hepatic dysfunction to severe end-stage liver disease. Cholestasis developed most commonly in neonate, ursodeoxycholic acid (UDCA) is widely used in adult with cholestatic and non-cholestatic liver diseases but there have been limited data on the effects in neonate with PNAC. This study was performed retrospectively to review all medical histories of the total 30 neonates with was administrated UDCA for treatment to parenteral nutrition associated cholestasis (PNAC) at Chungbuk National University Hospital NICU from April 2002 to December 2008. UDCA was administrated at bilirubin is over 2 mg/dl. The criterias for drug evaluation were included hepatic biochemical marker such as direct bilirubin, total bilirubin, AST, ALT, ALP and GGT, TPN therapy period, cholestasis development period, UDCA treatment period, UDCA dosage and adverse effect. In the results, Post-UDCA treatment significant was decreased direct bilirubin, total bilirubin, AST and ALP (p<0.05), and was decreased GGT (p>0.05) and slightly was increased ALT (p>0.05). Reffective timect biDCA was appear at mean $10.5{\pm}1.3$ days, iDCA administration period was mean $64.4{\pm}5.9$ days, cholestasis period was mean $71.9{\pm}6.4$ days and UDCA dosage was mean $22.9{\pm}0.9$ mg/kg/day. Common adverse effects is diarrhea, 5 patients arised mild diarrhea but it possible also related with increased enteral feeding. In conclusion, iDCA can decrease direct bilirubin that major parameter t bcholestasis and oher hepatic biochemical makers. UDCA is effective on PNAC without any serious side effect and cost-effective. Although no greatly shortening cholestasis period, but can protect to develop into severe liver disease and other complication or death. Based on these result, UDCA is recommended for treatment of cholestasis at direct bilirubin is over 2 mg/dl.
Hwang, Hyeon-Shik;Kim, Wang-Sik;Kim, Jeong-Moon;Mcnamara, James A. Jr.
The korean journal of orthodontics
/
v.40
no.1
/
pp.34-39
/
2010
Objective: The aim of the present study was to evaluate the changes in tooth mobility following orthodontic treatment and to obtain information regarding the guideline of retainer wear duration during the post-treatment period. Methods: The sample consisted of twenty patients who had been treated with edgewise appliances. The mobility of the maxillary teeth from the central incisor to the first molar was measured bilaterally by way of the $Periotest^{(R)}$, a non-invasive, electronic device that provides an objective measurement of the reaction of the periodontium to a defined impact load. Tooth mobility was monitored at the time of the removal of the orthodontic appliances and subsequently at three-month intervals during the two years following appliance removal. Results: Tooth mobility decreased rapidly for the first six months and then decreased at a slower rate during the next six months; no statistically significant decrease in mobility was observed during the second year following appliance removal. Conclusions: The results of the present study suggest that adequate tooth stabilization is critical during the first six months following appliance removal and that continued wearing of retainers is recommended at least until twelve months after the completion of orthodontic treatment.
The variety Tong-il from the IR667 lines have been reported as a variety resistant to the smaller brown planthopper, Laodelphax striatellus Fallen, in Korea. The resistance of Tong-il to the insect, however, have been mainly evaluated from a viewpoint of plant reaction of rice at the seedling stage. In this study, the nature of resistance of Tong-il to the insect was furthermore evaluated from the standpoints of feeding preference, antibiosis and plant tolerance in comparison with the resistant varieties Karsamba Red ASD-7 and Vellailangalayan. and the susceptible variety Jinheung which have been reported to the insect. The smaller brown planthopper much more prefered Tong-il for feeding than the resistant varieties ASD-7 and Vellailangalayan. The degree of antibiosis of Tong-il to the smaller brown planthopper was significantly lower than ASD-7 and Vellailangalayan. The results showed significantly lower nymphal mortality and higher adult emergence in Tong-il and Jinheung than other two resistant varieties. The degree o( tolerance of Tong-il to the insect was also lower than ASD-T and Vellailangalayan. Only. Tong-il seemed to be relatively more tolerable than Jinheung. The reason of showing resistant reaction of Tong-il in the mass screening test might be related with relatively lower feeding preference and more tolerance than Jinheung and other susceptible varieties.
In this study we conducted artificial hybridization between two sibling species, Rhodeus uyekii and R. notatus, and observed the morphological characteristics in early developmental stage. The two species showed difference in egg shape having rhombus and club respectively. The morphology of yolk of larva just hatched also showed well specific characteristics, that is the bean chaff type for R. uyekii (U type) and anchor type for R. notatus (N type). The rate of fertilization between female R. uyekii and male R. notatus (UN type) and between female R. notatus and male R. uyekii (NU type) were complete and its hatching rate were very high, 71.6% for UN type and 97.5% for NU type. The differences occurred in the yolk shape of hatched larvae for each combination of hybrids. In the group of UN, U type of larvae were found very rarely, but almost all the larvae showed intermediate shape polarized to NN type. Similar phenomenon was observed in the NU having intermediate polarized to UU, but without any NN type. These hybrids will be analyzed for their external morphology, sex ratio, the function of sexual organ and karyology after they grown up to adult.
Early life history of the endangered species, Gobiobotia macrocephala was investigated to provide baseline data for biological characteristics and recovery. Adult fish were sampled by spoon net at Hongcheon-gun, Gangwon-do, Korea in April to June 2010. The female's eggs were obtained by injecting Ovaprim and fertilized by dry method in the laboratory. The mature eggs were slightly adhesive and transparent with greyish and average 0.89${\pm}$0.04mm in diameter. The hatching of the embryo took place in about 107 hours after fertilization under water temperature of 23$^{\circ}C$ and newly hatched larvae were average 4.6${\pm}$0.16mm in total length. At 4 days after hatching, they were average 6.1${\pm}$0.20mm in total length and their yolk sacs were completely absorbed. From 15 days after hatching, they entered the juvenile stage and reached at 8.6${\pm}$0.67mm in total length. At the 100 days after hatching, their band patterns and external form were similar to those of adults, and they averaged 31.5${\pm}$3.32mm in total length.
Artemia often observed as a co-existing organism in the mass culture tank of marine rotifer. The rotifer and Atremia are commonly used as food organisms in the marine fish larvae rearing. In this study, interspecific relation between the rotifer Brachionus rotundiformis (formely called S-type) and anostracan Artemia of the two developmental stages (0 and 19 day old after hatching) were investigated in the larboratory. The population growth of B. rotundiformis and one of the stage (nauplius or adult) of Artemia in mixed culture was compared with that of each single species culture. Culture period was 16 days. Every two days, the number of organisms in each species was counted and transferred to a fresh medium containing $7{\times}10^5$cells/ml of food Nannochloropsis oculata. Culture volume, temperature, salinity and photoperiod were set at 40ml, $25^{\circ}C.$, 22ppt and 24h all dark except to observation time, respectively. The rotifer population growth was greatly decreased by co-existence with Artemia. The coexisting Artemia suppressed the rotifer population growth due to it's high filtering speed for food (N. oculata). This study suggested that contamination by Artemia must be prevented for the stable rotifer production in the rotifer mass culture tank.
Kim, In-Young;Kim, Jae-Moon;Jeong, Tae-Sung;Kim, Shin
Journal of the korean academy of Pediatric Dentistry
/
v.35
no.1
/
pp.110-117
/
2008
Clinical performance of resin-based composite material depends on case selection and on the proficiency of the operator. Especially, composite resin restoration on permanent first molar in children have more limitations clinically than adult patients according to stage of tooth eruption and patient's compliance. This study was designed to evaluate the clinical performance of posterior composite resin restoration in children after 5 years. 35 teeth of 16 patients who were received composite resin restoration on permanent first molar in Department of Pediatric dentistry, Pusan National University Hospital between January 2001 and December 2001 were evaluated based on Modified USPHS criteria. From the finding in this study, following conclusions can be made. 1. 6 teeth(17%) of 35 teeth was replaced, so 5-years survival rate of posterior composite resin restoration is 82.9%. 2. As results of each evaluation criteria, on color match, anatomic form, surface roughness, sensitivity/ discomfort, ideal A grade score was 86.2%, 93.1%, 86.2%, 86.2%, clinically accepted B grade score was 13.8%, 0%, 13.8%, 10.3%. On marginal adaptation and marginal discoloration, A grade score was 13.8%, 44.8% and B grade score was 79.3%, 34.5% and secondary caries rate was 20.7%. 3. 69.1% of teeth (20 teeth) was clinically accepted on all evaluation criteria.
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