• Title/Summary/Keyword: Administration Research

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Pregnancy rate in Hanwoo cows after timed artificial insemination using different sperm concentrations

  • Sung-Sik Kang;Sang-Rae Cho;Ui-Hyung Kim;Yonghwan Kim;Seok-Dong Lee;Myung-Suk Lee;Eunju Kim;Jeong-Il Won;Shil Jin;Hyoun-Ju Kim;Sungwoo Kim;Sun-Sik Jang;Seunghoon Lee
    • Journal of Animal Reproduction and Biotechnology
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    • v.39 no.1
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    • pp.40-47
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    • 2024
  • Background: Sperm quality and the number of sperm introduced into the uterus during artificial insemination (AI) are pivotal factors influencing pregnancy outcomes. However, there have been no reports on the relationship between sperm concentration at AI and sperm quality in Hanwoo cattle. In this study, we examined sperm quality and pregnancy rates after AI using sperm inseminated at different concentrations. Methods: We evaluated the motility, viability, and acrosomal membrane integrity of sperm at different concentrations (10, 15, 18, and 20 million sperm/straw) in 0.5-mL straws. Subsequently, we compared the pregnancy rates after AI with different sperm concentrations. Results: After freeze-thawing, sperm at the assessed concentrations showed similar viability and acrosomal membrane integrity. After AI, cattle in the 10 million group had significantly lower pregnancy rates compared to those in the 18 and 20 million groups. Conversely, there were no statistically significant variances observed between cattle in the 10 and 15 million groups. Conclusions: Sperm at concentrations of 10, 15, 18 and 20 million per straw exhibited comparable motility, viability, and acrosomal membrane integrity. However, a concentration of at least 18 million sperm per straw is required to achieve a consistent rate of pregnancy rate in Hanwoo cattle after AI.

Screening of Selected Korean Sweetpotato (Ipomoea batatas) Varieties for Fusarium Storage Root Rot (Fusarium solani) Resistance

  • Lee, Seung-yong;Paul, Narayan Chandra;Park, Won;Yu, Gyeong-Dan;Park, Jin-Cheon;Chung, Mi-Nam;Nam, Sang-Sik;Han, Seon-Kyeong;Lee, Hyeong-Un;Goh, San;Lee, Im Been;Yang, Jung-Wook
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.407-416
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    • 2019
  • A common post-harvest disease of sweetpotato tuber is root rot caused by Fusarium solani in Korea as well as the other countries. Storage root rot disease was monitored earlier on sweetpotato (Ipomoea batatas) in storehouses of different locations in Korea. In the present study, an isolate SPL16124 was choosen and collected from Sweetpotato Research Lab., Bioenergy Crop Research Institute, NICS, Muan, Korea, and confirmed the identification as Fusarium solani by conidial and molecular phylogenetic analysis (internal transcribed spacer ITS and translation elongation factor EF 1-α gene sequences). The isolate was cultured on potato dextrose agar, and conidiation was induced. The fungus was screened for Fusarium root rot on tuber of 14 different varieties. Among the tested variety, Yenjami, Singeonmi, Daeyumi, and Sinjami showed resistant to root rot disease. Additionally, the pathogen was tested for pathogenicity on stalks of these varieties. No symptom was observed on the stalk, and it was confirmed that the disease is tissue specific.