Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Pregnancy rate in Hanwoo cows after timed artificial insemination using different sperm concentrations

  • Sung-Sik Kang (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Sang-Rae Cho (Animal Genetic Resources Research Center, National Institute Animal Science, Rural Development Administration) ;
  • Ui-Hyung Kim (Poltury Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Yonghwan Kim (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Seok-Dong Lee (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Myung-Suk Lee (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Eunju Kim (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Jeong-Il Won (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Shil Jin (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Hyoun-Ju Kim (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Sungwoo Kim (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Sun-Sik Jang (Hanwoo Research Institute, National Institute Animal Science, Rural Development Administration) ;
  • Seunghoon Lee (Animal Biotechnology Division, National Institute Animal Science, Rural Development Administration)
  • Received : 2024.02.19
  • Accepted : 2024.03.19
  • Published : 2024.03.31
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Abstract

Background: Sperm quality and the number of sperm introduced into the uterus during artificial insemination (AI) are pivotal factors influencing pregnancy outcomes. However, there have been no reports on the relationship between sperm concentration at AI and sperm quality in Hanwoo cattle. In this study, we examined sperm quality and pregnancy rates after AI using sperm inseminated at different concentrations. Methods: We evaluated the motility, viability, and acrosomal membrane integrity of sperm at different concentrations (10, 15, 18, and 20 million sperm/straw) in 0.5-mL straws. Subsequently, we compared the pregnancy rates after AI with different sperm concentrations. Results: After freeze-thawing, sperm at the assessed concentrations showed similar viability and acrosomal membrane integrity. After AI, cattle in the 10 million group had significantly lower pregnancy rates compared to those in the 18 and 20 million groups. Conversely, there were no statistically significant variances observed between cattle in the 10 and 15 million groups. Conclusions: Sperm at concentrations of 10, 15, 18 and 20 million per straw exhibited comparable motility, viability, and acrosomal membrane integrity. However, a concentration of at least 18 million sperm per straw is required to achieve a consistent rate of pregnancy rate in Hanwoo cattle after AI.

Keywords

Acknowledgement

This study was conducted with the support of the Cooperative Research Program for Agriculture Science & Technology Development project: "A study on the puberty and proper breeding time in Hanwoo cow," PJ016809, RDA, Korea.

References

  1. Almquist JO. 1975. Effect of sperm numbers on fertility of frozen bull spermatozoa in skim milk diluent. J. Dairy Sci. 58: 420-422.  https://doi.org/10.3168/jds.S0022-0302(75)84581-4
  2. Andersson M, Taponen J, Koskinen E, Dahlbom M. 2004. Effect of insemination with doses of 2 or 15 million frozen-thawed spermatozoa and semen deposition site on pregnancy rate in dairy cows. Theriogenology 61:1583-1588.  https://doi.org/10.1016/j.theriogenology.2003.09.006
  3. Baruselli PS, Ferreira RM, Sa Filho MF, Bo GA. 2018. Review: using artificial insemination v. natural service in beef herds. Animal 12(s1):s45-s52.  https://doi.org/10.1017/S175173111800054X
  4. Camus A, Gonzalez A, Schmitt E. 2016. Novel protein-free semen medium improves fertility potential of frozen bovine sperm. Anim. Reprod. Sci. 169:108. 
  5. Harstine BR, Utt MD, DeJarnette JM. 2018. Review: integrating a semen quality control program and sire fertility at a large artificial insemination organization. Animal 12(Suppl 1): s63-s74.  https://doi.org/10.1017/S1751731118000319
  6. Januskauskas A, Gil J, Soderquist L, Haard MG, Haard MC, Johannisson A, Rodriguez-Martinez H. 1999. Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in Sweden. Theriogenology 52:641-658.  https://doi.org/10.1016/S0093-691X(99)00159-4
  7. Januskauskas A, Soderquist L, Haard MG, Haard MC, Lundeheim N, Rodriguez-Martinez H. 1996. Influence of sperm number per straw on the post-thaw sperm viability and fertility of Swedish red and white A.I. bulls. Acta Vet. Scand. 37: 461-470.  https://doi.org/10.1186/BF03548086
  8. Kang SS, Cho SR, Hwang SM, Kim UH, Kim KW. 2021. A study on the breeding status and improvement of reproduction rate of repeat-breeder in Hanwoo. J. Korea Acad. Ind. Coop. Soc. 22:806-812. 
  9. Kang SS, Kim UH, Lee MS, Lee SD, Cho SR. 2020. Spermatozoa motility, viability, acrosome integrity, mitochondrial membrane potential and plasma membrane integrity in 0.25 mL and 0.5 mL straw after frozen-thawing in Hanwoo bull. J. Anim. Reprod. Biotechnol. 35:307-314.  https://doi.org/10.12750/JARB.35.4.307
  10. Kang SS, Lee MS, Kim UH, Lee SD, Yang BC, Yang BS, Cho SR. 2019. Effect of Optixcell and Triladyl extenders on frozenthawed sperm motilities and calving rates following artificial insemination in Hanwoo. Korean J. Agric. Sci. 46:195-204. 
  11. Kathiravan P, Kalatharan J, Karthikeya G, Rengarajan K, Kadirvel G. 2011. Objective sperm motion analysis to assess dairy bull fertility using computer-aided system--a review. Reprod. Domest. Anim. 46:165-172.  https://doi.org/10.1111/j.1439-0531.2010.01603.x
  12. Kommisrud E, Steine T, Graffer T. 1996. Comparison of fertility rates following insemination with different numbers of spermatozoa per insemination dose of frozen bovine semen. Reprod. Domest. Anim. 31:359-362.  https://doi.org/10.1111/j.1439-0531.1996.tb00085.x
  13. Kumar P, Saini M, Kumar D, Balhara AK, Yadav SP, Singh P, Yadav PS. 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. 159: 38-45.  https://doi.org/10.1016/j.anireprosci.2015.05.010
  14. Layek SS, Mohanty TK, Kumaresan A, Parks JE. 2016. Cryopreservation of bull semen: evolution from egg yolk based to soybean based extenders. Anim. Reprod. Sci. 172:1-9.  https://doi.org/10.1016/j.anireprosci.2016.04.013
  15. Mohanty TK, Lone SA, Kumaresan A, Bhakat M, Kumar R, Baithalu RK, Sinha R, Paray AR, Yadav HP, Sahu SK, Mohanty AK. 2018. Sperm dosage and site of insemination in relation to fertility in bovines. Asian Pac. J. Reprod. 7:1-5.  https://doi.org/10.4103/2305-0500.220977
  16. Perumal P, Selvaraju S, Selvakumar S, Barik AK, Mohanty DN, Das S, Das RK, Mishra PC. 2011. Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred Jersey bulls on sperm parameters and conception rates. Reprod. Domest. Anim. 46:636-641.  https://doi.org/10.1111/j.1439-0531.2010.01719.x
  17. Pickett BW, Hall RC, Lucas JJ, Gibson EW. 1964. Influence of sperm number on fertility of frozen bovine semen. J. Dairy Sci. 47:916-919.  https://doi.org/10.3168/jds.S0022-0302(64)88802-0
  18. Richardson BN, Larimore EL, Walker JA, Utt MD, DeJarnette JM, Perry GA. 2017. Comparison of fertility of liquid or frozen semen when varying the interval from CIDR removal to insemination. Anim. Reprod. Sci. 178:61-66.  https://doi.org/10.1016/j.anireprosci.2017.01.010
  19. Sales JN, Neves KA, Souza AH, Crepaldi GA, Sala RV, Fosado M, Campos Filho EP, de Faria M, Sa Filho MF, Baruselli PS. 2011. Timing of insemination and fertility in dairy and beef cattle receiving timed artificial insemination using sex-sorted sperm. Theriogenology 76:427-435.  https://doi.org/10.1016/j.theriogenology.2011.02.019
  20. Stevenson JS, Higgins JJ, Jung Y. 2009. Pregnancy outcome after insemination of frozen-thawed bovine semen packaged in two straw sizes: a meta-analysis. J. Dairy Sci. 92:4432-4438.  https://doi.org/10.3168/jds.2009-2304
  21. Wiltbank JN and Parish NR. 1986. Pregnancy rate in cows and heifers bred to bulls selected for semen quality. Theriogenology 25:779-783.  https://doi.org/10.1016/0093-691X(86)90093-2
  22. Yang BC, Kang SS, Kim UH, Jang SS, Yang BS, Lee SD, Cho SR. 2017. Surveys on reproduction status and calf production of Hanwoo farmers. J. Emb. Trans. 32:171-176.