• Journal of Periodontal and Implant Science
• /
• 제37권1호
• /
• pp.53-64
• /
• 2007

The aim of this study was to examine the possibility of periodontal ligament regeneration when autotransplantation was used by the periodontal ligament fibroblasts cultured on the acellular dermal matrix in teeth without a periodontal ligament. One minipig was used in this study. The mandibular and maxillary permanent incisors were ex-tracted for the culture of the periodontal ligament cells. The roots of the unextracted teeth were classified into a positive control group, in which the normal periodontal ligament was preserved. The roots of the extracted teeth were divided into the following two groups: The negative control group, in which the periodontal ligament had been removed and the acellular dermal matrix was not applied; and an experimental group, in which the periodontal ligament had been removed and periodontal ligament fibroblast cultured on an acellular dermal matrix was applied. The prepared teeth were transplanted, and completely submerged using physical barrier membranes. The animal was sacrificed 4 weeks after the autotransplant. The transplanted teeth were examined histologically. In this study, the periodontal ligament was normal in the positive control group, and ankylosis was discovered on the denuded root surface in the negative control group. Periodontal ligament-like connective tissue was found adjacent to the denuded root and the new cementum-like layer of hard tissue was formed in the experimental group. These results suggest that the periodontal ligament fibroblasts cultured on the acellular dermal matrix may play a role in regenerating the periodontal ligament-like tissue with new cememtum-like tissue formation.

• 대한의생명과학회지
• /
• 제16권4호
• /
• pp.365-376
• /
• 2010

The purpose of this study was to clarify the effect of light emitting diode (LED) irradiation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Twenty-four male Sprague-Dawley rats were divided into four groups: excision (Ex), excision-LED irradiation (Ex-LED), diabetes + excision (DM) and diabetes + excision + LED irradiation (DM-LED). Diabetes was induced in rats by streptozotocin (STZ) injection (70 mg/kg, single dose) and 6 mm punch excision wounds were created on the back after shaving hair. The LED-irradiated rats were treated to a daily dose of $5\;J/cm^2$ LED (630 nm) light for 11 days after surgery, and were killed at day 1, 3, 7 and 11. The lesion and adjacent skin tissues were excised, fixed with 10% buffered formalin and embedded with paraffin. For evaluation of wound healing, hematoxylin-eosin (HE) and Masson trichrome staining were performed. Mast cells (MCs) were stained with toluidine blue (pH 0.5) and quantified using a computerized image analysis system. The proliferation activity of keratinocyte in skin tissues was analyzed on sections immunostained with proliferative cell nuclear antigen (PCNA). The results showed that wound healing rate, collagen density and neo-epidermis length, number of PCNA-positive cells, fibroblasts and mast cells were significantly higher in the LED-irradiated rats than in the DM and Ex rats throughout the periods of experiment. Exceptionally, the number of MCs was significantly lower at day 11 compared with day 7 after surgery in the all groups. These findings suggest that the LED irradiation may promote the tissue repair process by accelerating keratinocyte and fibroblast proliferation and collagen production in normal rats as well as in diabetic rats, and MCs may play an important role at an early stage of skin wound healing in normal and diabetic rats.

• 한국지반공학회지:지반
• /
• 제13권5호
• /
• pp.101-124
• /
• 1997

The root pile system is insitu soil reinforcement technique that uses a series of reticulately installed micropiles. In terms of mechanical improvement by means of grouted reinform ming elements, the root pile system is similar to the soil nailing system. The main difference between root piles and soil nailing are due to the fact that the reinforcing bars in root piles are normally grouted under high pressure and that the alignments of the reinforcing members differ. Recently, the root pile system has been broadly used to stabilize slopes and retain excavations. The accurate design of the root pile system is, however, a very difficult tass owing to geometric variety and statical indetermination, and to the difficulty in the soilfiles interaction analysis. As a result, moat of the current design methods have been heavily dependent on the experiences and approximate approach. This paper proposes a quasi-three dimensional method of analysis for the root pile system applied to the stabilization of slopes. The proposed methods of analysis include i) a technique to estimate the change in borehole radium as a function of the grout pressure as well as a function of the time when the grout pressure is applied, ii) a technique to evaluate quasi -three dimensional limit-equilibrium stability for sliding, iii) a technique to predict the stability with respect to plastic deformation of the soil between adjacent root piles, and iv) a quasi -three dimensional finite element technique to compute stresses and dis placements of the root pile structure barred on the generalized plane strain condition and composite unit cell concept talon형 with considerations of the group effect and knot effect. By using the proposed technique to estimate the change in borehole radius as a function of the grout pressure as well as a function of the time, the estimations are made and compar ed with the Kleyner 8l Krizek's experimental test results. Also by using the proposed quasi-three dimensional analytical method, analyses have been performed with the aim of pointing out the effects of various factors on the interaction behaviors of the root pile system.

• Journal of Gastric Cancer
• /
• 제2권4호
• /
• pp.213-220
• /
• 2002

Purpose: The cDNA microarray provides a powerful alternative with an unprecedented view in monitoring geneexpression levels and leads to discoveries of regulatory pathways involved in complicated biological processes. Our aim is to explore the different gene-expression patterns in gastric adenocarcinomas. Materials and Methods: By using a cDNA microarray representing 4,600 cDNA clusters, we studied the expression profiling in 10 paired gastric adenocarcinoma samples and in adjacent noncancerous gastric tissues from the same patients. Alterations in the gene-expression levels were confirmed by Vsing Northern blots and reverse-transcription PCR (RT-PCR) in all of 4 randomly selected genes. Results: Genes those were expressed differently in cancer ous and noncancerous tissues were identified. 44 (of which 26 were known) and 92 (of which 43 were known) genes or cDNA were up- and down-regulated, respectively, in more than $80\%$ of the gastric adenocarcinoma samples. In cancer ous tissues, genes related to gene/protein expression, cellcycle regulation, and metabolism were mostly up-regulated whereas genes related to the oncogene/tumor suppressor gene, cell structure/motility, and immunology were mostly down-regulated. The semi-quantitative RT-PCR results for the four genes we tested were consistent with the array findings. Conclusions: These results provide not only a new molecular basis for understanding the biological properties of gastric adenocarcinomas but also a useful resource for future development of therapeutic targets and diagnostic markers for gastric adenocarcinomas.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권2호
• /
• pp.989-997
• /
• 2014

AMFR, autocrine motility factor receptor, also called gp78, is a cell surface cytokine receptor which has a dual role as an E3 ubiquitin ligase in endoplasmic reticulum-associated degradation. AMFR expression is associated with tumor malignancy. We here investigated the clinical significance of AMFR and its role in metastasis and prognosis in gastric cancer. Expression of AMFR, E-cadherin and N-cadherin in cancer tissues and matched adjacent normal tissues from 122 gastric cancer (GC) patients undergoing surgical resection was assessed by immunohistochemistry. Levels of these molecules in 17 cases selected randomly were also analysed by Western blotting. AMFR expression was significantly increased in gastric cancer tissues, and associated with invasion depth and lymph node metastasis. Kaplan-Meier analysis showed AMFR expression correlated with poor overall survival and an increased risk of recurrence in the GC cases. Cox regression analysis suggested AMFR to be an independent predictor for overall and recurrence-free survival. E-cadherin expression was decreased in gastric cancer tissues; conversely, N-cadherin was increased. Expression of AMFR negatively correlated with E-cadherin expression, whereas N-cadherin expression showed a significant positive correlation with AMFR expression. AMFR might be involved in the regulation of epithelial-mesenchymal transition, with aberrant expression correlating with a poor prognosis and promoting invasion and metastasis in GCs.

• Journal of Microbiology and Biotechnology
• /
• 제9권6호
• /
• pp.832-838
• /
• 1999

Two different types of lipases (lipase I and lipase II) secreted into culture medium by Rhizopus sp. L-I were purified using a hydrophobic chromatography and were partially characterized. Both enzymes were monomeric as revealed by SDS-PAGE and gel filtration. The molecular masses of the enzymes were identified as 45 kDa (lipase I) and 69 kDa (lipase II). The isoelectric points were estimated to be 3.6 and 5.2 for lipase I and lipase II, respectively. pH and temperature activity optima for lipase I were as 7.5 and $50^{\circ}C$, respectively, whereas the corresponding parameters for lipase II were 6.0 and $45^{\circ}C$. The amino terminal sequences of lipase I and lipase II, determined by Edman degradation, were found to be Leu-Val-Met-Ile-Gln-Arg and Leu-Val-Met-Lys-Gln-Arg, respectively. By western blotting analysis, the two lipases were found to have a common antigenic determinant. Immuno-electron cytochemistry conducted with polyclonal anti-lipase I antibody indicated the enzyme located in both the periplasm and the adjacent vesicles of fungal hyphae. Fortunately, the sites on the cell envelope where lipase was exported into the culture medium was also identified.

• Journal of Computing Science and Engineering
• /
• 제4권2호
• /
• pp.173-187
• /
• 2010

Recent technological advances in mobile communication systems have made explosive growth in the number of mobile device users worldwide. One of the most important issues in designing a mobile computing system is location management of users. The hierarchical systems had been proposed to solve the scalability problem in location management. The scalability problem occurs when there are too many users for a mobile system to handle, as the system is likely to react slow or even get down due to late updates of the location databases. In this paper, we propose a top-down clustering algorithm for hierarchical location database systems in a wireless network. A hierarchical location database system employs a tree structure. The proposed algorithm uses a top-down approach and utilizes the number of visits to each cell made by the users along with the movement information between a pair of adjacent cells. We then present a modified algorithm by incorporating the exhaustive method when there remain a few levels of the tree to be processed. We also propose a capacity constraint top-down clustering algorithm for more realistic environments where a database has a capacity limit. By the capacity of a database we mean the maximum number of mobile device users in the cells that can be handled by the database. This algorithm reduces a number of databases used for the system and improves the update performance. The experimental results show that the proposed, top-down, modified top-down, and capacity constraint top-down clustering algorithms reduce the update cost by 17.0%, 18.0%, 24.1%, the update time by about 43.0%, 39.0%, 42.3%, respectively. The capacity constraint algorithm reduces the average number of databases used for the system by 23.9% over other algorithms.

• Journal of Microbiology
• /
• 제43권2호
• /
• pp.133-143
• /
• 2005

Shigellosis is a global human health problem. Four species of Shigella i.e. S. dysenteriae, S. flexneri, S. boydii and S. sonnei are able to cause the disease. These species are subdivided into serotypes on the basis of O-specific polysaccharide of the LPS. Shigella dysenteriae type 1 produces severe disease and may be associated with life-threatening complications. The symptoms of shigellosis include diarrhoea and/or dysentery with frequent mucoid bloody stools, abdominal cramps and tenesmus. Shigella spp. cause dysentery by invading the colonic mucosa. Shigella bacteria multiply within colonic epithelial cells, cause cell death and spread laterally to infect and kill adjacent epithelial cells, causing mucosal ulceration, inflammation and bleeding. Transmission usually occurs via contaminated food and water or through person-to-person contact. Laboratory diagnosis is made by culturing the stool samples using selective/differential agar media. Shigella spp. are highly fragile organism and considerable care must be exercised in collecting faecal specimens, transporting them to the laboratories and in using appropriate media for isolation. Antimicrobial agents are the mainstay of therapy of all cases of shigellosis. Due to the global emergence of drug resistance, the choice of antimicrobial agents for treating shigellosis is limited. Although single dose of norfloxacin and ciprofloxacin has been shown to be effective, they are currently less effective against S. dysenteriae type 1 infection. Newer quinolones, cephalosporin derivatives, and azithromycin are the drug of choice. However, fluoroquinolone-resistant S. dysenteriae type 1 infection have been reported. Currently, no vaccines against Shigella infection exist. Both live and subunit parenteral vaccine candidates are under development. Because immunity to Shigella is serotype-specific, the priority is to develop vaccine against S. dysenteriae type 1 and S. flexneri type 2a. Shigella species are important pathogens responsible for diarrhoeal diseases and dysentery occurring all over the world. The morbidity and mortality due to shigellosis are especially high among children in developing countries. A recent review of literature (KotIoff et al.,1999) concluded that, of the estimated 165 million cases of Shigella diarrhoea that occur annually, $99\%$ occur in developing countries, and in developing countries $69\%$ of episodes occur in children under five years of age. Moreover, of the ca.1.1 million deaths attributed to Shigella infections in developing countries, $60\%$ of deaths occur in the under-five age group. Travellers from developed to developing regions and soldiers serving under field conditions are also at an increased risk to develop shigellosis.

• 한국정보과학회논문지:정보통신
• /
• 제31권3호
• /
• pp.335-346
• /
• 2004

본 논문에서는 편향 자기 경로 제어 방법을 사용하는 새로운 고성능 고장 감내 스위칭 망을 제시하고, 이 스위칭 망의 고장 진단 방법을 제안한다. 우리는 기본 스위치인 벤얀 망의 각 스위칭 단계들이 하나의 순환 군의 나열로 구성되어 있다는 사실과 자기 경로제어 관점에서 인접한 스위칭 단계들 간에 준동형(Homomorphism)이 존재하기 때문에, 모든 각 단계의 순환 군이 이 다음 단계의 부분군이고, 이들 단계들로 구성된 펙터 군이 존재한다는 성질과 사용하여, 결함이 생긴 링크들을 우회하는 대체 경로들로 추가한 링크들을 포함하는 모든 링크들을 사용하는 확장 벤얀 망인 다단 스위칭 망을 제시한다. 그리고 이 망의 성능을 시뮬레이션을 통해서 분석하여, 성능의 우수함을 보인다. 또한 이들 다중 경로를 제공하는 스위칭 망을 사용하는데 필수적인 결함 진단법을 제시한다.

• 한국항공우주학회지
• /
• 제31권5호
• /
• pp.1-9
• /
• 2003

본 연구에서는 비정렬격자계를 사용하여 덮개 꼬리 로터의 제자리 비행에 대한 압축성, 비정성 유동을 해석하였다. 유동계산을 위한 수치적 기법으로는 셀 중심에 기초한 유한체적법과 내재적인 시간적분법을 사용하였다. 계산은 로터의 한 블레이드에 대해 수행되었으며, 블레이드와 블레이드 사이에는 주기적 경계조건을 설정하였다. 덮개가 없는 로터 형상에 대한 성능은 실험 결과와 잘 일치함을 보였다. 덮개를 포함하는 로터 형상에 대한 계산은 비교된 실험 형상의 불확실성을 고려하여 추력이 일치하는 피치각을 가지는 경우에 대해 수행하였으며, 자세한 유동은 실험결과와 잘 일치함을 확인할 수 있었다. 그 결과로부터 본 방법이 블레이드 끝단간극을 포함하는 복잡한 3차원 덮개 꼬리 로터 형상 해석에 매우 유용하게 사용될 수 있음을 알 수 있었다.