• Korean Journal of Pharmacognosy
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• v.46 no.4
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• pp.283-288
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• 2015

In this study, we investigated anti-obesity effect of isoegomaketone (IK) isolated from leaves extract of Perilla frutescens (L.) Britt. cv. We verified differentiation and lipid accumulation by Oil Red O staining in 3T3-L1 cells after IK treatment with differentiation media. IK inhibited mRNA expression of adipocyte specific genes that were related with differentiation of 3T3-L1 cells. We confirmed the effects of IK on body weight and visceral fat mass in obese mice. Mice were randomly divided into three groups; normal diet group (ND), high-fat diet group (HFD) and high-fat diet with IK group (HFD-IK). The obesity mice were induced by feeding the 45% high-fat diet to the C57BL/6J mice during 4 weeks. After HFD-IK was orally administered 10 mg/kg of IK. As a result, the body weight of HFD and HFD-IK was increased 2.4 times and 1.7 times of ND, respectively. Also visceral fat mass of HFD was increased 24 times but in the case of HFD-IK was increased to 13 times in comparison with ND. Taken together, our findings suggest that IK reduced differentiation and adiogenesis in 3T3-L1 cells, decreased the body weight and visceral fat mass in obesity mice. These results suggest that IK may have a potential benefit as anti-obesity material.

• Journal of Practical Agriculture & Fisheries Research
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• v.26 no.2
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• pp.31-40
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• 2024

This study was indicated that both potato leaves and stems contained certain amount of alkaloids compounds which digested in the rumen of Hanwoo steers. Three candidate plant byproducts such as potato, tomato, and eggplant were collected and dried for making feed additives. Among the candidate byproducts, potato extract was contained 2.05±0.27mg/g of α-chaconine and 0.60±0.08mg/g of α-solanine. These glycoalkaloids was potentially activated as functional compounds to skeletal muscle in beef cattle. However, tomato and eggplant byproducts were not detected any alkaloids compounds. After potato byproducts collected and dried for 24 hours, dry matter of potato byproduct were used as feeding study for evaluating active dose titrations. For evaluating dietary rate of rumen microbes, potato dry matter were treated with in situ hybridization for 72 hours using annulated Hanwoo steers. The glycoalkaloids contained potato dry matter were digested until 12 hours after treatment. Blood concentration of glycoalkaloids were not detected after 24 hours treatment of potato dry matter. These data indicated that potato byproduct were digested with rumen microbes for 24 hours after treatments.