• Korean Journal of Acupuncture
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• 제30권2호
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• pp.97-103
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• 2013

Objectives : To observe the changes in the expression of nNOS, iNOS, eNOS and NO by the needle insertion on river points, one of the five transport points of three yang meridians of the foot. Methods : Based on rats, needle was inserted on both left and right sides of ST41, BL60 and GB38 and retained for five minutes. After the retention, blood was drawn via cardiac puncture and tissues from each point around meridian vessels were extracted to observe the changes in the expression of nNOS, iNOS, eNOS and NO. Results : In terms of the effect on expression of nNOS within tissues, ST41 showed significant decrease based on the results of immunohistochemistry. In terms of the effect on expression of iNOS within tissues, none of the experimental groups showed any significant change compared to the Normal group. Regarding expression of eNOS within tissues, GB38 showed significant increase based on the results of immunohistochemistry. In terms of the effect on NO creation in tissues, none of the experimental groups showed any significant change compared to the Normal group. Conclusions : The effect of needle insertion on the river points of three yang meridians of the foot on the function of nNOS, iNOS, eNOS and NO could be observed, and based on this study, it is considered that the effect of needle stimulation on the changes of nervous system could be found out through additional research.

• 혜화의학회지
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• 제23권1호
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• pp.157-166
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• 2014

상완골 간부 골절에 동반된 요골신경 손상을 가진 1명의 환자를 대상으로 "내경(內經)"의 독취양명치법(獨取陽明治法)에 따라 침구치료, 약침치료, 물리치료와 한약치료를 병행하였다. 손목관절의 하수 및 근력약화에 대한 치료성적으로 평가하기 위해 완관절의 운동범위와 능동저항검사를 통한 상대적인 지수를 평가하였으며, 요골신경마비로 인한 장악력의 약화정도는 악력측정기를 사용하여 평가하였다. 수지의 비증의 정도는 Numeric Rating Sclae(NRS) 사정법에 따라 평가하였다. 환자는 발병일로부터 약 6주째에 첫 회복징후를 보이기 시작하였으며, 발병이로부터 약 11.5주째에 가벼운 물건에 대한 집게운동이 가능하며 일상생활을 하는데에 큰 지장이 없는 수준으로 회복되었다. 이는 상완골 간부 골절이 동반된 요골신경손상의 자연회복시기보다 빠른 것으로, "내경(內經)"의 독취양명치법(獨取陽明治法)에 따른 한방치료요법이 상완골 골절에 동반된 요골신경손상의 치료에 효과가 있음을 알 수 있었다.

• Korean Journal of Acupuncture
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• 제29권3호
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• pp.406-420
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• 2012

Objectives : Scutellaria barbata has been widely used in oriental medicine used for treatment of acute and chronic inflammatory diseases. In this study, to investigate the protective effect of Scutellaria barbata on type I allergic response, we determined whether Scutellaria barbata inhibits early or late allergic responses. Methods : To assess the effect of Scutellaria barbata Pharmacopuncture Extract(SB) in RBL-2H3 cells, we investigated the levels of the markers of degranulation such as ${\beta}$-hexosaminidase and histamine, inflammatory mediator such as IL-4, TNF-${\alpha}$, PGE2 and cysLT, and mRNA expression of cytokines and enzymes. In addition, we determined the levels of intracellular ROS by DCFH-DA assay and the free radical scavenging activity by DPPH method. Results : We found that SB suppressed the release of ${\beta}$-hexosaminidase and histamine and the production of IL-4, TNF-${\alpha}$, PGE2 and cysLT in RBL-2H3 by the antigen stimulation. SB also significantly inhibited the enzyme mRNA expressions, such as HDC2, COX-1, COX-2, 5-LOX and iNOS2, along with reduced cytokine mRNA expressions, such as IL-$1{\beta}$, IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ and GM-CSF in RBL-2H3. In addition, SB suppressed the levels of intracellular ROS. Conclusions : Our results indicate that SB protects against type I allergic response and exert an anti-inflammatory effect through the inhibition of degranulation, inflammatory mediator release and mRNA expression of cytokines and enzymes.

• 대한한의학원전학회지
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• 제27권3호
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• pp.101-122
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• 2014

Objectives : The comparison of symptoms of hypoadrenocorticism with symptoms according to spleen and kidney show how many points of similarity they are. And by analysis of symptoms of hypoadrenocorticism it was examined that Kidney Jeonggyeok help to cure many different symptoms including symptoms of hypoadrenocorticism. Methods : The symptoms of hypoadrenocorticism were compared and analyzed according to standards of the book of korean traditional medical pathology. First, this study was designed to show to found out how many symptoms of hypoadrenocorticism belong to symptoms of kidney qi-deficiency and slpeen qi-deficiency and how similar they are. Second, the symptoms of kidney qi deficiency were compared with the indicant of kidney Jeonggyeok suggested by two of korean traditional doctors to find out kidney Jeonggyeok can help to cure symptoms of kidney qi deficiency. And then on the basis of those, the symptoms of hypoadrenocorticism were compared with the symptoms of kidney qi deficiency. Results : The hypoadrenocorticism seems to be kidney yang(陽) deficiency. But because hypoadrenocorticism doesn't have body temperature decline, symptoms of hypoadrenocorticism are more similar symptoms of kidney qi deficiency than symptoms of kidney yang deficiency. The symptoms of hypoadrenocorticism seems to be correlated with the functions of spleen. But because of vomiting and pigmentation(skin and mucous membrane), they seem to have less to do with the functions of spleen than the functions of kidney. The comparison analysis of indicant of Kidney Jeonggyeok and symptoms of kidney qi deficiency shows that acupuncture stimulation help to cure kidney qi deficiency and hypoadrenocorticism. Conclusions : Symptoms of hypoadrenocorticism are expected to be more similar symptoms of kidney qi deficiency. And Kidney Jeonggyeok is helpful for treatment of Kidney qi deficiency and hypoadrenocorticism.

• 대한한방내과학회지
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• 제39권3호
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• pp.293-301
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• 2018

Objective: The purpose of this clinical trial is to examine the efficacy and safety of electroacupuncture on liver cirrhosis patients suffering from muscle cramps. Methods: A total of 14 liver cirrhosis patients with muscle cramps were treated by electroacupuncture three times a week for four weeks (12 times total). The electrical stimulation was a frequency of 100 Hz. Evaluation of the muscle cramps was measured by a questionnaire of subjective symptoms. The patients' condition was evaluated four times over a period of eight weeks. We also assessed the deterioration of liver disease using the Child-Pugh score and blood tests. Results: This study proves that four weeks of electroacupuncture treatment is effective in reducing the frequency of muscle cramps and that the effects of electroacupuncture treatment lasted during another four-week follow-up period after treatment. Conclusions: This study suggests that electroacupuncture treatment is beneficial for improving muscle cramps in liver cirrhosis patients (p=0.000). Electroacupuncture is thought to be a safe treatment for liver cirrhosis patients with muscle cramps without contributing to the worsening of liver function. Further study with a larger sample size is needed to confirm our findings.

• 대한물리치료과학회지
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• 제9권2호
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• pp.111-122
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• 2002

In oriental medicine, manual-acupuncture and electroacupuncture (EA) have been widely utilized to cure several inflammatory diseases such as arthritis. We designed this experiment to find neurochemical mechanism related to electroacupuncture induced anti-inflammatory effect on mouse air pouch model. EA with both low frequency (1 Hz) and high frequency (120 Hz) was treated after induction of inflammation in air pouch using injection of zymosan. To verify the role of opioid system in electroacupuncture-induced anti-inflammatory effect, naloxone (10 mg/kg) was pretreated. In addition, idazoxan (5 mg/kg) was pre-treated to evaluate the possible effect of endogenous adrenergic system in autonomic system on EA induced anti-inflammatory effect. As results of this study, naloxone pretreatment did not change the anti-inflammatory effect evoked by high frequency EA, while low frequency EA(1 Hz) induced anti-inflammatory effect was dramatically suppressed by naloxone pretreatment. These data indicated that endogenous opioid system might be extensively involve in anti-inflammatory effect evoked by not high frequency, but low frequency EA. However, idazoxan pretreatment did not produce any modulatory effect on both low and high frequency EA induced anti-inflammatory effect, suggesting that EA induced anti-inflammatory effect was not mediated by endogenous adrenergic system. In conclusion, these data strongly suggested that EA induced anti-inflammatory effect is mediated by endogenous opioid system, not endogenous adrenergic system.

• 한국임상수의학회지
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• 제34권4호
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• pp.287-290
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• 2017

A 6-month-old, 4.1 kg female Dachshund dog presented with intermittent non-weight bearing lameness of the right thoracic limb. Radiographs revealed caudolateral luxation of the right radial head and a shortened right ulna compared to the contralateral limb. Bone lengthening by distraction of the ulna using the Ilizarov technique was performed following ulnar osteotomy. The rate of distraction was 1.5 mm per day, adjusted a total of 3 times daily for a total distraction distance of 10 mm. The Ilizarov fixator was removed four weeks after surgery. The patient showed knuckling due to radial nerve injury that occurred during limb-lengthening. Corrective osteotomy was performed using a plate and pin for the luxation and deformity of the right radial head. The luxation of the radial head was successfully reduced following surgery. However, the knuckling persisted after surgery. Rehabilitation for radial nerve injury was performed using heat therapy, massage, a passive range of motion exercises, water treadmill exercises, neuromuscular electrical stimulation, leash-walking, and acupuncture. 15 months after surgery, the patient showed satisfactory weight-bearing ambulation without recurrence of lameness. The use of the Ilizarov technique is a good surgical option for the treatment of a patient with congenital elbow luxation.

• 동의신경정신과학회지
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• 제22권1호
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• pp.1-12
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• 2011

Objectives : This study was conducted to assess effects of oriental medicine music therapy on heart rate variability(HRV) in normal subjects under mental stress. Methods : 34 healthy volunteers(control group:17, experimental group:17) participate in this study. The volunteers were randomly allocated into two groups. After 10-minutes rest period and instrumentation, both groups performed a mental stress test for 12-minutes. HRV was recorded before and after the mental stress. And then the experimental group applied oriental medicine music therapy for 10-minutes. In the control group, they rested for 10-minutes. And HRV was recorded. Results : After giving mental stress stimulation to both groups, LF and LF/HF ratio were significantly increased and HF, mean HRV were decreased. In experimental group, LF, LF/HF ratio were significantly decreased and HF, SDNN were significantly increased after applying oriental medicine music therapy. In control group, LF was significantly decreased. And LF/HF ratio was decreased and HF, SDNN were increased, but it was not significant. Conclusions : This results show that oriental medicine music therapy could be relatively useful to decrease sympathetic activity and prevent the alteration of autonomic nervous system due to mental stress.

• 한국의사학회지
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• 제26권1호
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• pp.107-132
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• 2013

Objectives : This study was performed to investigate the research trends of hypertension in Traditional Chinese Medicine(TCM), and it aims to promote hypertension research in Korean Medicine. Methods : We first collected more than 1,900 papers about hypertension research, and finally selected 108 papers related to this study. They were analyzed by the annul situation, the subject of the study, the perspective of TCM, the study stream, the research fields, the diagnostic patterns, and the distinguishing treatments of TCM. Results : The first review about hypertension in TCM was performed in 1963, and the number of studies has increased since 2000s. Doctors and researchers in China tried various diagnostic patterns to treat the patient because the diagnostic patterns were not unified. For this reason, most researches were reported to the diagnostic patterns and the treatments. Also we have discovered the diversity in treatments methods such as not only the typical herbal medicine, acupuncture, and Qigong but also specific treatments like Chuna, herbal acupoints stimulation, footbath, and pillow. Conclusions : Based on the results of this study, it can be proposed as follows : First, the various approach about hypertension in Korean Medicine is required. Second, the domestic research is needed to be extended to the external treatments as the distinguishing treatments of TCM.

• 대한본초학회지
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• 제33권3호
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• pp.19-24
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• 2018

Objectives : Hedgehog skin is one of the animal medicines in Traditional Korean Medicine for hematochezia and hemorrhoids. In this study, we examined cytotoxicity and anti-inflammatory effects. Methods : Cytotoxicity of hedgehog skin extracts was measured by MTT assay in vitro. We investigated the inhibition of lipopolysaccharide (LPS) stimulated nitric oxide (NO) production in RAW 264.7 cells. The phosphorylation of mitogen-activated protein kinases (MAPKs) was measured by western blot. And we observed the effect of hedgehog skin extracts on the expression of IL-6 genes using real time PCR. Results : As a result of MTT assay for cytotoxicity, there were no significant differences between non-treatment group and hedgehog skin extracts treatment groups. $500{\mu}g/m{\ell}$ of hedgehog skin extracts treatment significantly decreased nitric oxide production in comparison with non-treatment in LPS-induced RAW 264.7 cells. In measurement of the phosphorylation of MAPKs using western blot analysis, LPS stimulation increased the phosphorylation of MAPKs and $500{\mu}g/m{\ell}$ of hedgehog skin extracts treatment decreased the phosphorylation of ERK1, ERK2 and p38 significantly. But there were no significant differences the phosphorylation of JNK1 and JNK2. As a result of confirmation of the IL-6 mRNA gene expression using real time PCR, IL-6 mRNA gene expressions were significantly decreased in $50{\mu}g/m{\ell}$, $100{\mu}g/m{\ell}$ and $500{\mu}g/m{\ell}$ hedgehog skin extracts treated groups by comparison with non-treatment group. Conclusion : These results could provide a mechanistic explanation for the anti-inflammatory effects of the hedgehog skin.