• 제목/요약/키워드: Activity concentration

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Corrosion of Stainless Steel Pipes Buried in the Soils of Seoul Metropolitan During One Year (1년 동안 서울지역 토양에 매설된 스테인리스강의 부식)

  • Hyun, Youngmin;Kim, Heesan;Kim, Young-Ho;Jang, Hyunjung;Park, Youngbog;Choi, Youngjune
    • Corrosion Science and Technology
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    • v.11 no.2
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    • pp.56-64
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    • 2012
  • Factors affecting corrosion of stainless steels such as pH, oxidation and redox potential (ORP), soil resistivity, water content of soil, chloride ion concentration, bacteria activity, and corrosion potential have been investigated using soil analysis, bacterial analysis, surfacial analysis, and analysis of corrosion potentials of several stainless steels buried in 8 sites of Seoul metropolitan for one year. Corrosion potential was affected by occurrance of corrosion as well as bacteria activity but the behavior of corrosion potential with time is different depending on occurrance of corrosion and bacteria activity. The main factor affecting corrosion of stainless steels in soil is level of chloride ion concentration which is also a main factor affecting corrosion of stainless steels in chloride containing drinkable water. Furthermore, guideline of stainless steels in drinkable water is concluded to be applicable to that in soil by the results from surfacial analysis.

Toxicity Evaluation of Complex Metal Mixtures Using Reduced Metal Concentrations: Application to Iron Oxidation by Acidithiobacillus ferrooxidans

  • Cho, Kyung-Suk;Ryu, Hee-Wook;Choi, Hyung-Min
    • Journal of Microbiology and Biotechnology
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    • v.18 no.7
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    • pp.1298-1307
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    • 2008
  • In this study, we investigated the inhibition effects of single and mixed heavy metal ions ($Zn^{2+},\;Ni^{2+},\;Cu^{2+},\;and\;Cd^{2+}$) on iron oxidation by Acidithiobacillus ferrooxidans. Effects of metals on the iron oxidation activity of A. ferrooxidans are categorized into four types of patterns according to its oxidation behavior. The results indicated that the inhibition effects of the metals on the iron oxidation activity were noncompetitive inhibitions. We proposed a reduced inhibition model, along with the reduced inhibition constant ($\alpha_i$), which was derived from the inhibition constant ($K_I$) of individual metals and represented the tolerance of a given inhibitor relative to that of a reference inhibitor. This model was used to evaluate the toxicity effect (inhibition effect) of metals on the iron oxidation activity of A. ferrooxidans. The model revealed that the iron oxidation behavior of the metals, regardless of metal systems (single, binary, ternary, or quaternary), is closely matched to that of any reference inhibitor at the same reduced inhibition concentration, $[I]_{reduced}$, which defines the ratio of the inhibitor concentration to the reduced inhibition constant. The model demonstrated that single metal systems and mixed metal systems with the same reduced inhibitor concentrations have similar toxic effects on microbial activity.

Physicochemical Properties of Bokbunja Jelly Containing Fermented Sea Tangle Saccharina japonica Powder (유산균 발효다시마(Saccharina japonica)를 첨가한 복분자 젤리의 이화학적 품질특성)

  • Choi, Ji-Won;Lee, Yeon-Ji;Moon, Soo-Kyung;Kim, Yong-Tae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.4
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    • pp.408-416
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    • 2022
  • This study investigated the physicochemical properties and physiological activities of jelly prepared from gelatin, sugar, bokbunja extract, and different amounts (0, 0.5, 1, 2 and 3%) of fermented sea tangle Saccharina japonica powder (FSP). The jelly moisture, pH, and sugar content slightly increased with increasing the FSP content. Hardness, springiness, gumminess, chewiness, and cohesiveness also increased with increasing FSP concentration. Jelly antioxidant activity did not change significantly with increasing FSP. In contrast, alcohol dehydrogenase and aldehyde dehydrogenase activities in the jellies increased significantly with increasing FSP concentration. β-secretase inhibitory activity in jellies also increased with increasing FSP concentration. Jellies containing 0.5 or 1% FSP achieved the highest overall sensory acceptance scores. Taken together, these data indicate that addition of FSP to jelly appears to improve its quality and physiological activities.

Effects of Seeding Microorganisms, Hydrazine, and Nitrite Concentration on the Anammox Activity (혐기성 암모늄 산화균의 활성에 대한 식종미생물, 히드라진 및 아질산성 질소 농도의 영향)

  • Jung, Jin-Young;Kang, Shin-Hyun;Kim, Young-O;Chung, Yun-Chul
    • Journal of Korean Society on Water Environment
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    • v.21 no.5
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    • pp.477-483
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    • 2005
  • Anammox (Anaerobic Ammonium Oxidation) bacteria is recently discovered microorganism which can oxidize ammonium to nitrogen gas in the presence of nitrite under anaerobic conditions. The anammox process can save an energy for nitrification and need not require a carbon source for denitrification, however, the start-up periods takes a long time more than several months due to the long doubling time (approximately 11 days). In order to find the effects of seeding microorganisms, hydrazine, and nitrite concentration on the enhancement of the anammox activity, five kinds of microorganisms were selected. Among the several kinds of seeding microorganisms, the granule from acclimated microorganisms treating high concentration of ammonia nitrogen (A-1) and sludge from piggery wastewater treatment plant (A-2) were found to have a high anammox activity. In the case of A-1, the maximum nitrogen conversion rate represented 0.4 mg N/L-hr, and the amount of nitrite utilization was high compared to those of other seeding microorganisms. The A-4 represented a higher nitrogen conversion rate to 0.7 mg N/L-hr although the ammonium concentration in the serum bottle was high as 200 mg/L. Meanwhile, the anaerobic granule from UASB reactor treating distillery wastewater showed a low anammox activity due to the denitrification by the remained carbon sources in the granule. Hydrazine, intermediate product in anammox reaction, enhanced the anammox activity by representing 1.4 times of nitrogen gas was produced in the test bottle than that of control, when 0.4 mM of $N_2H_4$ was added to serum bottle which contains 5 mM of nitrite. The high concentration of nitrite (10 mM) resulted in the decrease of the anammox activity by showing lower production of nitrogen gas compared to that of 5 mM addition of nitrite concentration. As a result of FISH (Florescence In-Situ Hybridization) experiment, the Amx820 probe showed a more than 13% of anammox bacteria in a granule (A-1).

Glutathione Concentration as Affected by Sulfate Supply Level and its Relationship with Sulfate Uptake and Assimilatory Enzymes Activity in Rape Plants (유채에서 황 공급수준에 따른 글루타치온 함량의 변화가 황산염 흡수 및 동화관련 효소활력에 미치는 영향)

  • Li, Lu-Shen;Kim, Tae-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.1
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    • pp.15-24
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    • 2011
  • The glutathione (GSH) concentration in leaves of different maturities and roots of forage rape (Brassica napus L.) supplied with four levels of external $SO_4^{2-}$ (0, 0.1, 1.0 and 2.0 mM) supply were measured. The relationships of GSH concentration with $SO_4^{2-}$ uptake, ATP sulfurylase (ATPS) and O-acteylserine (thiol) lyase (OASTL) activity were also assessed. The $SO_4^{2-}$ uptake increased in parallel with the external $SO_4^{2-}$ supply, while protein concentration was not significantly changed. The ATPS activity increased continuously with decreasing $SO_4^{2-}$ supply from 2.0 to 0 mM, while the OASTL activity decreased significantly only at S-deficient conditions (0 and 0.1 mM). The GSH concentration in the young leaves, middle leaves and roots continuously increased (except for between 1.0 and 2.0 mM in the middle leaves and roots) as the external S supply was increased, but no significant changes occurred in the old leaves. The increased endogenous GSH concentration, affected by the $SO_4^{2-}$ supply level, was significantly related with the decrease in ATPS activity in both leaves and roots, and the decrease in OASTL activity only in leaves..

Fermentation of Momordica charantia Extract using Leuconostoc mesenteroidesies and Physiological Activity of Product (Leuconostoc mesenteroidesies 균주를 이용한 여주 추출물 발효 및 생산물의 생리활성 특성)

  • Kang, Jung-Hoon
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1250-1259
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    • 2018
  • In this study, Momordica charantia (MC) fermented with Leuconostoc mesenteroides (MC-LM) were assessed for the antioxidant and the antidiabetic activities. Antioxidant activities of MC and MC-LM were evaluated using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical. Although MC-treated groups showed little activity, 47% of activity was observed at $500{\mu}g/mL$ concentration for MC-LM and increased significantly(p<0.05) as MC-LM concentration increased. MC-LM more effectively inhibited the oxidative damage of DNA by peroxyl radical than MC and the inhibition of the strand breakage increased significantly as MC-LM concentration increased(p<0.05). Measuring the inhibition of ${\alpha}-glucosidase$ activity, which is closely related to the regulation of blood sugar, resulted in MC reduced the activity of ${\alpha}-glucosidase$ by 30% at 8 mg/mL and MC-LM at the same concentration by 60%. In addition, the effect of MC-LM on the cell viability of alloxan-treated RIN-m5F resulted in a significant increase in cell survival(p<0.05) in the group treated with MC-LM and a 20% increase in the concentration of $1000{\mu}g/mL$. As a result of insulin secretion by alloxan-treated RIN-m5F cell, the level of insulin secretion tended to increase in all group treated with MC-LM. At the concentration of $1000{\mu}g/mL$, the insulin secretion was increased by 15% in MC-LM group than in MC group. In conclusion, the results of this study suggest that fermented bitter gourd has antioxidant and antidiabetic effects.

The Evaluation on the Effectiveness of Zingiber mioga extract as a Cosmetic Material through Verification of Cosmeceutical Activations (양하(Zingiber mioga) 추출물의 화장품 약리활성 검증을 통한 화장품 소재로서의 유효성 평가)

  • Um, Mi Sun
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.5
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    • pp.1088-1099
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    • 2020
  • The purpose of this study was to confirm the possiblility of Zingiber mioga as a cosmetic material. For this we carried out biologically activated material characteristic evaluation about anti-oxidant, anti-inflammatory, wrinkle reduction effects using Zingiber mioga extract. To carry out this experiment, we extracted Zingiber mioga extract from Zingiber mioga flowers exract (ZMF) and Zingiber mioga leaves exract (ZML) with 70% ethanol. In order to evaluate the anti-inflammatory effect, we tested the toxicity and the hindrance activity to nitric oxide of samples using macrophages (RAW 264.7 cells). After we measured DPPH radical scavenging activity, ABTS+ radical scavenging activity and superoxide dismutase (SOD)-like activity of the Zingiber mioga extracts, we knew that they increased depending on their concentration. ZMF showed higher antioxidant activity than ZML after the measurement of ABTS+ radial scavenging activity and superoxide desmutase (SOD)-like activity. According to the measurement result of Nitric oxide inhibition activity we knew that ZMF reduced NO productions in a concentration-dependent manner. After the measurement of the biosynthesis quantity of pro-collagen type-1, we knew that its excellent effect appeared 110% or more at the concentration of 25 ㎍/ml. And at the same concentration, the result of the measurement of metalloprotease (MMP)-1 inhibition effect showed the 20% activation. In conclusion, ZMF is expected to be applied as a cosmetic material for wrinkle reduction. Zingiber mioga is believed to be used as a natural cosmetic material because it has been proven to have antioxidant, anti-inflammatory, and wrinkle-improving effects.

Prediction of Water Activity for Gelatinized Model Foods (모형식품의 수분활성도 예측)

  • Jung, Seung-Hyeon;Chang, Kyu-Seob;Park, Young-Deok
    • Korean Journal of Food Science and Technology
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    • v.25 no.2
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    • pp.94-97
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    • 1993
  • This study was to predict water activity of gelatinized model foods containing moisture, protein and starch with different concentration of humectants such as sodium chlorife and sucrose. The water activity of each samples were determinded by electrical hygrometry. The degree of lowering water activity in model foods with humectant solutions was following order as NaCl>sucrose. Model food $P_2S_1$ was predominant in depression of water activity by humectants than other model foods. The multiple regression equations between water activity and different humectants concentration, compositions and solution ratio of model foods were obtained and $R^2$ values were higher than 0.91.

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Effect of medicinal plant extract for hangover relief (약용식물 추출물의 숙취 해소 효과에 관한 연구)

  • Hyun, Chang-Su;Park, Garyoung;Oh, Young Mi;Lee, Youngjae;Han, Chang-Hoon
    • Korean Journal of Veterinary Research
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    • v.54 no.4
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    • pp.233-238
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    • 2014
  • The present study was performed to evaluate the effect of medicinal plant extract on relieving hangovers in mice administered alcohol. The animals were divided into three groups. Each group was treated with fermented plant extract, non-fermented plant extract, or water 30 min after consuming ethanol (2 mL/kg). A locomotor activity test showed that all groups had decreased motor activity until 40 min after plant extract administration. The mice treated with water had lower motor activity until 100 min post-administration. However, the group treated with non-fermented plant extract showed increased motor activity 40 min post-administration, and the higher activity level was maintained until 120 min post-administration. The animals treated with fermented plant extract had a level of motor activity between those of the groups treated with water or non-fermented plant extract. Blood was collected from each mouse 120 min post-administration and aldehyde concentration was measured. The group treated with non-fermented plant extract had a significantly higher (p < 0.05) aldehyde concentration than the other groups. These results demonstrate that the non-fermented medicinal plant extract helped alleviate hangovers 40 min after administration by reducing aldehyde concentrations in the blood.

Optimization for the Antibacterial Activity of Konjak Jelly using Evolutionary Operation-Factorial Design Technique (Evolutionary Operation-Factorial Design Technique를 이용한 곤약의 항균활성 최적화)

  • Lee, Nan-Hee;Choi, Won-Seok;Choi, Ung-Kyu
    • The Korean Journal of Food And Nutrition
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    • v.31 no.2
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    • pp.272-277
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    • 2018
  • This research was conducted to elucidate the optimum conditions for the antibacterial activity of konjak jelly using the evolutionary operation-factorial design technique. In the first set of experiments, concentration of a coagulation agent, soaking liquid, and temperature of water were set to 0.4%, $0.6{\times}10^{-2}N$, and $65^{\circ}C$ as a central point, respectively. The highest antibacterial activity was acquired at E21, in which the number of bacteria was 1.25 log cfu/g. Because the code of changes in the main effect was (-), it could be decided that the central point of the first set was not the optimum point. Although antibacterial activity in the second set was improved, the values of the main effect were higher than that of changes in the mean effect. The central point of third set was concentration of coagulation agent 0.8%, concentration of soaking liquid $1.0{\times}10^{-2}N$, and temperature of water $65^{\circ}C$. It was found that the antibacterial activity of central point in the third set was highest among all the tested set. Further, all the necessary conditions were appropriate to reach the optimum condition. The antibacterial activity of the central point in third set was more than 1,000 times higher than that of E11, in first set.