• 약학회지
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• 제31권5호
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• pp.266-272
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• 1987

It is reasonably well known that there is a relationship between myelin formation and methylation of myelin basic protein in nerve cells. One of the suggestions is that arginine methylation of myelin basic protein could be of aid in the conjugation of myelin protein with the nonpolar lipid to form myelin. Abnormality in methylation of myclin basic protein might induce the neurological diseases in experimental animals as well as in human being. In the biological system, the methylation reaction is catalyzed by protein methaylse I using S-adenosyl-L-methionine as methyl donor. In this study, we examined the changes of S-adenosyl-L-methionine concentration and protein methylase I activity in developing rat brain tissues. The results are sumraerized as followings: (1) In brain tissues of fetus rat, the concentration of S-adenosyl-L-methionine was gradually decreased until to birth. However, the concentration in brain tissues of infant rat was suddenly increased at 7th day(just before myelination occur) birth. (2) Protein methylase I activity was decreased until to birth in brain of fetus rat and increased temporally just after birth, However, the enzyme activity showed no changes around 7th day after birth.

• 한국환경보건학회지
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• 제23권4호
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• pp.121-126
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• 1997

Peroxidase as one of the organic enzyme catalyst is useful for the oxidation treatment of various aromatic compounds such as phenols. The peroxidase content of Oenanthe javanica was 24.85 unit/g-fw in leaf, 5.74 unit/g-fw in stem, and 34.69 unit/g-fw in root respectively. The crude peroxidase extracted from Oenanthe javanka can be kept under low temperature (-70$\circ$C) condition for 6 months with the maximum 1% activity reduction. The optimum conditions of removal for 100 ppm phenol was pH 6, hydrogen peroxide 3.5 mM, peroxidase activity 8 unit/ml, temperature 20$\circ$C respectively. In the wide range of concentration from 50 ppm to 750 ppm phenol reveals average 54% removal rate under the same peroxidase activity (8 unit/ml) and different amount of hydrogen peroxide proportional to phenol concentration. Especially at the concentration of 100 ppm the maximum phenol removal rate was 72%.

• 한국환경보건학회지
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• 제37권2호
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• pp.133-140
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• 2011

Antimicrobial activity and bactericidal activity of Caesalpinia sappan L. extracts were investigated against five food-borne pathogens, E. coli, S. aureus, S. typhimurium, B. cereus and L. monocytogenes. Methanol extract of Caesalpinia sappan L. revealed antimicrobial activities against five pathogens. In particular, by paper disc diffusion the highest activity was shown against L. monocytogenes. Antimicrobial activities of methanol extracts showed the most potent activities, but hexane fraction had no activity. Fractions of ethyl acetate and butanol turned out to have higher antimicrobial activities against Gram(+) bacteria than Gram(-) bacteria. The minimum inhibitory concentration against five food-borne pathogens was 1.563 mg/ml on Gram(+) bacteria and 3.125 mg/ml on Gram(-) bacteria. The result of antimicrobial activity in a shaking flask method showed that bacterial growth rate fell by more than 99.999% at 3.125 mg/ml of methanol extract. The highest rate of viable reduction (99.998%) was shown at 0.781 mg/ml of methanol extract against L. monocytogenes. After five minutes of reaction between test strains and methanol extracts, the growth rates of five kinds of bacteria were reduced by more than 99.999% at a concentration of 100 mg/ml. Therefore, it is suggested that methanol extracts of Caesalpinia sappan L. can be developed as a natural sanitizer or disinfectant.

• 사상체질의학회지
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• 제17권2호
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• pp.64-73
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• 2005

1. Objectives The purpose of this study was to investigate the effects of the enzyme activity of Palmulgunja-tang with administered orally solution on cytochrome P450 isozyme 2. Methods This study was carried on through following methods. We treated the rat with the {3-naphthoflavone (${\beta}NF$) of 80mg/kg for 3 days i.p injection. Firstable, microsomal protein was separated and total intracellular protein test was done. Then GOT and GPT were measured and assay of cytochrome P450 IAI/2 enzyme activity was performed according to the method of EROD and MROD. (Ethoxyresorufin-O-deethylase(EROD) activity was used to measure cytochrome P450 lAI activity and methoxyresorufin O-demethylase(MROD) activity was used to measure cytochrome P450 lA2 activity. ) 3. Results and Conclusions 1) PGT recovered the liver damage on ${\beta}NF$ inducible CYP IAI/2 by pre-post and high-low condition. 2) At concentration of post-treated 50mg!kg of PGT, the inhibiting of $\betaNF$ metabolites to liver of rat cytochrome P450 lAl was inhibited by 53.0% respectively. 3) PGT showed 36.0% inhibition of ${\beta}NF$-induced lA2 activity at the concentration post-treated 50mg/kg.

• Journal of Plant Biology
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• 제30권3호
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• pp.173-179
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• 1987

The effect of polyamine, Ca2+ and calmodulin on GS ($\beta$-glucan synthetase) activity was studied in Daucus carota root. The Ca2+ is shown to have no effect on the GS activity whereas the GS II activity is increased in response to increase in concentration of the Ca2+. When the protoplasts are cultured, for 4 days, the GS II activity increases as a tunction of time and reachs a maximum after 3 days at a time when the network of cellulose microfibrils is known to be synthesized. The effect of the Ca2+ and 1mM spermine on the GS II activity turns out to be synergistic, especially more synergistic at lower concentration of the Ca2+. The GS II activity seems to be enhanced by the Ca2+. The GS II activity in the protoplast treated by the calcium channel blocker, verapamil, turns out to be lower than that of the control. Cumulative results suggest that the Ca2+ stimulates the cell wall regeneration via enhancement of the GS II activity responsible for synthesizing the cell wall component throught synergistic effect with spermine.

• Archives of Pharmacal Research
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• 제21권6호
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• pp.718-722
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• 1998

Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part of Solanum lyratum Thunb. by the activity-guided fractionation employing carbon te trachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1mcM to 50mcM. Further studies revealed that at the concentration of 10mcM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.

• Asian-Australasian Journal of Animal Sciences
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• 제14권7호
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• pp.966-969
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• 2001

Some researchers reported that beef marbling was improved by the supplementation of organic zinc to a diet satisfying zinc requirement. We studied the relationship between serum zinc concentration and marbling score or serum adipogenic activity in 40 fattened steers. To determine serum adipogenic activities of the steers, preadipocytes were cultured in medium containing the serum samples during differentiation. Although serum zinc concentration was not related to beef marbling score, it was positively correlated to adipogenic activity. Then, we studied the effect of zinc on adipocyte differentiation. Zinc was added into the medium with the similar methods except the addition of fattened calf serum. The activity of glycerophosphate dehydrogenase, a marker of adipocyte differentiation, was significantly increased by the addition of zinc in culture with or without insulin. These results suggest that zinc possibly improved beef marbling through increasing adipogenic activity during fattening.

• 대한약리학회지
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• 제6권1호
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• pp.1-7
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• 1970

The effects of ouabain and diphenylhydantoin on ATPase activity in rat erythrocyte membranes were studied and also influence of K on ATPase activity was studied. The ATPase activity of rat erythrocyte membrane has been shown to consist of two components. The first component requires the Mg but occurs in the absence of Na or K (Mg-ATPase) and is not inhibited by ouabain and stimulated by diphenylhydantoin. The second component requires the presence of Mg and also Na or K (Na-K-Mg-ATPase). It is inhibited by ouabain and is stimulated by diphenylhydantoin in low Na concentration and inhibited in high Na concentration. K inhibit Na-K-Mg-ATPase which is inhibited by ouabain. Ouabain and diphenylhydantoin show reversed effect to Na-K-Mg-ATPase activity. It suggest that the therapeutic effect of diphenylhydantoin on digitalis induced cardiac arrhythmia may be resulted from their effect on ion transport mechanism of cell membrance. And the relevance of these findings to the action of ouabain and diphenylhydantoin on membrane transport mechanism is discussed.

• 한국약용작물학회지
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• 제19권5호
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• pp.388-394
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• 2011

Mume fructus is the roasted fruits of Prunus mume and has been used as traditional chinese medicine. In this study, mume fructus extracts were prepared by three different methods including hot water extract (sample1), fermentation extract using Lactobacillus (Sample2-LP and sample-LA) and ethanol extract (sample3). Total polyphenol and flavonoid contents were improved by fermentation process, compared to water extract. Sample 3 showed the highest activity in DPPH radical scavenging. The cytotoxicity of the sample 2-LP was in the range of 83.3% cell viability at $300{\mu}g/m{\ell}$ concentration. Mume fructus extracts in a concentration-dependent manner inhibited melanogenesis and NO synthesis was inhibited. Specifically, the extracts fermented by L. plantarum (sample2-LP) showed higher anti-oxidant activity, anti-inflammatory and skin whitening activity than other extracts. It suggests that sample 2-LP could be potentially used as a resource of materials for functional cosmetics.

• 한국식품영양학회지
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• 제12권2호
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• pp.199-203
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• 1999

To evaluate antidental caries activity of polylysine cell growth and acid production of Streptococcus mutans and lactobacillus acidophilus were microbiologically monitored in anaerobic broth system containing various concentration of polylysine. The pH and heat stability of polylysine having antimicrobial activity were also examined. Two tested microbes were fairly well grown in broth containing polylysine 0.1mg/ml however inhibited at 1 and 2mg/ml of polylysine concentration. Especially lag times of Strep-tococcus mutans and Lactobacillus acidophilus were prolonged to about hour at 1.0 and 2.0 mg/ml of poly-lysine. acid production of Streptococcus mutans and lactobacillus acidophilus was also decreased by poly-lysine. Antimicrobial activity of polylysine was not affected by the change of pH and the heat treat-ment.