• 대한한방내과학회지
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• 제20권1호
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• pp.143-152
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• 1999

Dansam, the root of Salvia miltiorrhiza Bge, (Labiatae), has a bitter taste and a slightly 'cold' property, and is nontoxic. In the present study, effect of Dansam on nitric oxide (NO) generation from peritoneal macrophags was examined. Dansam had no effect on NO generation by itself, whereas recombinant interferon-${\gamma}\;(rIFN-{\gamma})$ alone had modest activity. When Dansam was used in combination with $rIFN-{\gamma}$, there was a marked cooperative induction of NO generation in a dose-dependent manner, The optimal effect of Dansam on NO generation was shown at 6 hr after treatment with $rIFN-{\gamma}$. Furthermore, the effect of Dansam was mainly dependent on Dansam-induced tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ secretion. These results suggest that Dansam induces NO generation from macrophages by the result of Dansam-induced $TNF-{\alpha}$ secretion.

• 한국식품영양과학회지
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• 제20권4호
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• pp.401-407
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• 1991

동물실험을 통하여 새로이 밝혀진 항암제(anti-initiator/anti-ptomotor)인 CLA는 grilled ground beef에서 처음 분리되었다. CLA는 grilled ground beef 외에도, cheese 및 이들 관련식품에 많이 존재한다. CLA는 반추동물의 위에 서식하는 혐기성 bacteria에 의해 linoleic acid로부터 생성되며, 식품 가공 중에서도 생성된다. 이것은 또한 in vivo에서 linoleic acid의 carbon centered free radical 형태의 산화에 의해 생성되기도 한다. CLA는 아주 강력한 항산화제임이 밝혀져, 지금까지 알려지 있지 않았는 free radical에 대응하여 membrane을 보호하는 in situ defense mechanism 역할을 한다. 이는 또한 cytochrome P450 isozyme의 활성을 저해하는 반면, ODC 효소 활성 역시 저해한다. 그래서, 적어도 CLA의 이 세가지 biological activity가 CLA 항암기작에 관여하는 것으로 생각된다.

• 한국조명전기설비학회지:조명전기설비
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• 제9권2호
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• pp.23-28
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• 1995

원적외선이 생체에 미치는 영향을 관찰하기 위하여 포유동물인 흰쥐를 대상으로 실험을 실시하였다. 원적외선이 발생되도록 특수설계된 장치를 이용하여 흰쥐의 생리상태, 생식활동 및 생존능력에 변화와 차이를 유발시키는지의 여부를 관찰한 결과, 물과 먹이의 소모량, 체중, 수태율, 출산율 및 새끼의 성비(性比)에 있어서 자연상태와 동일하였기 때문에 원적외선이 하등의 유해작용을 발생시키지 않는다는 사실을 확인하였고, 가혹한 실험상황에서 흰쥐의 생존력이 자연상태의 흰쥐보다 약 122% 증가한다는 결과를 얻으므로써 원적외선이 흰쥐의 체력과 지구력을 증강시키는 역할을 한다는 새로운 사실을 관찰하였다.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1395-1400
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• 2024

The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin.

• 대한한의학회지
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• 제29권1호
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• pp.95-105
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• 2008

Objectives : Although herbal medicines containing flavonoids have been reported to exert anti-tumor activities, it has not been explored whether Hwang-Jeong (Polygonati sibirici Rhizoma, PsR) exerts anti-tumor activity in human glioma. The present study was therefore undertaken to examine the effect of PsR on cell viability and to determine its underlying mechanism in A172 human glioma cells. Methods : Cell viability was estimated by MTT assay. Reactive oxygen species generation and mitochondrial membrane potential were measured by the fluorescence dyes. The phosphorylation of kinases was evaluated by western blot analysis and caspase activity was estimated using colorimetric assay kit. Results : PsR resulted in loss of cell viability in a dose- and time-dependent manner. PsR did not increase reactive oxygen species (ROS) generation and the PsR-induced cell death was also not affected by antioxidants, suggesting that ROS generation is not involved in loss of cell viability. Western blot analysis showed that PsR treatment caused rapid reduction in phosphorylation of extracellular signal-regulated kinase (ERK) without changes in p38 and Jun-NH2-terminal kinase (JNK). U0126, an inhibitor of ERK, increased the PsR-induced cell death, but inhibitors of p38 and JNK did not affect the cell death. PsR induced depolarization of mitochondrial membrane potential. Caspase activity was not stimulated by PsR and caspase inhibitors did not prevent the PsR-induced cell death. Conclusion : Taken together, these findings suggest that PsR results in human glioma cell death through caspaseindependent mechanisms involving down-regulation of ERK.

• 대한교통학회지
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• 제30권3호
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• pp.107-118
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• 2012

본 연구는 대도시 화물자동차의 화물통행수요 추정을 위한 목적으로 대도시 화물자동차의 통행수요특성을 분석하고자 하였다. 대도시 화물자동차의 통행수요특성은 활동발생과 도착지선택, 그리고 통행사슬행태를 중심으로 분석하였다. 분석결과 출발존의 서비스업 사업체수가 대도시 화물자동차의 활동발생과 도착지 선택에서 주요한 영향을 미치는 요인으로 분석되었다. 그리고 도착지 유형이 도소매업체이고, 운송품목이 음식료품의 경우 통행사슬 내 통행수가 가장 많은 것으로 드러났다. 향후 대도시 화물자동차 통행수요추정시 이러한 변수와 운송품목을 고려할 필요가 있을 것으로 판단된다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.265-271
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• 2013

Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG $0.2{\mu}M$, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.

• 동의생리병리학회지
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• 제22권5호
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• pp.1322-1329
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• 2008

Mulberry has been reported to contain wide range of polyphenols and have chemopreventive activity. However, little has been known regarding the effect of mulberry fruits on cell viability in human glioma cells. The present study was undertaken to examine the effect of mulberry fruit (Mar; Fructus) on cell viability and to determine its underlying mechanism in human glioma cells. Cell viability and cell death were estimated by MTT assay and trypanblue exclusion assay, respectively. Reactive oxygen species (ROS) generation was measured using the fluorescence probe DCFH-DA. The mitochondrial transmembrane potential was measured with $DiOC_6$(3). Bax expression and cytochrome c release were measured by Western blot analysis. Caspase activity was estimated using colorimetric kit. Mori Fructus resulted in apoptotic cell death in a dose- and time-dependent manner. Mori Fructus increased ROS generation and the Mori Fructus-induced cell death was also prevented by antioxidants, suggesting that ROS generation plays a critical role in Mari Fructus-induced cell death. Western blot analysis showed that Mori Fructus treatment caused an increase in Bax expression, which was inhibited by the antioxidant N-acetylcysteine (NAC). Mori Fructus induced depolarization of mitochondrial membrane potential and its effect was inhibited by the antioxidants NAC and catalase. Mori Fructus induced cytochrome c release, which was inhibited by NAC. Caspase activity was stimulated by Mori Fructus and caspase inhibitors prevented the Mori Fructus-induced cell death. These findings suggest that Mori Fructus results in human glioma cell death through ROS-dependent mitochondrial pathway in human glioma cells.

• 문화기술의 융합
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• 제10권2호
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• pp.349-356
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• 2024

2025년, 우리나라는 노인 20.6%로 초고령사회로 진입할 것으로 전망되고 있다. 이에 본 연구는 지속적인 건강 관리를 위하여 디지털 기술과 높은 교육 수준을 지닌 베이비 붐 세대를 대상으로 '인지·정서 향상 챗봇 융합제품'의 사용에 따른 전후 건강상태 변화를 측정하였다. 연구대상은 건강공동체 23개소 중에서 서울지역의 중·고등학교 이상의 학력으로, 스마트기기 사용에 능숙하며, 자발적 사용 경험에 따른 데이터 제공 의지가 있는 대상자 50명을 선별하였다. 자료 수집 기간은 2023년 7월 24일부터 10월 5일까지이다. 50명의 신청자 중 탈락자와 소수의 남자를 제외한 43명의 베이비 부머 세대 여성을 대상으로 분석하였다. 연구결과 챗봇 사용 후 신체활동은 43.5.21(±1310.39) MET, 우울 정도는 6.84(±3.53) 통계적으로 유의하게 차이가 있었다. 그리고, 챗봇 사용 전후로 두 변수간에 통계적으로 유의한 상관성은 없었다. 신체활동과 우울척도에서만 유의한 효과가 나타난 것은 챗봇이 특정 건강 측면에 미치는 효과가 있을 수 있음과 각각에 다른 영향을 미쳤음을 시사한다. 향후 연구에서는 더 많은 변수와 더 다양한 건강 지표를 고려하여 챗봇의 효과를 더 깊이 분석할 필요가 있다.

• 한국의류산업학회지
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• 제10권5호
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• pp.702-712
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• 2008

The aim of this study is to develop Casual Hanbok design made of Hanji yarn textiles for New Silver generation women. The New silver generation is a coined word which has meaning of a newly silver generation and it is distinguished from pre-silver generation. New silver generation is a generation that is independent and given active role by their age groups and generational characteristic. The concept of silver generation was introduced from maturity market in Japan. This study was based on analysis about their preference of forms, colors, and materials of Casual Hanbok. The survey target were 270 female over age 50 living in Daejeon City. The analysis methods used frequency and percentage. The results were summarized as following. Although almost of them haven't worn Casual Hanbok, they had the positive recognition on wearing one. They preferred longer length jacket to traditional Korean style, shorter length of skirt. They also preferred the traditional sleeve shape and knotted buttons. On the foundation of this fact, 2 styles consist of 5 Casual Hanbok design items made of functional material - Hanji yarn textiles that have antimicrobial, deodorant, quick drying, far infrared radiation which are not harmful to health - for New silver women were suggested. One style was 3 items - blouse, vest, skirt - which are the sense of Korean tradition with activity by patch. The other was 2 items - jacket and skirt - which are the sense of Korean tradition with modern way by quilting. Lastly, these garments were evaluated by 13 experts, they were satisfied with 2 styles and all items. As this study were based on the Elderly women living in Daejeon city, it had the limitation on applying of preference styles to all the New silver generation.