• Archives of Pharmacal Research
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• v.25 no.4
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• pp.556-559
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• 2002

The aim of this study was to gain information on the effects of the crystal form of corticosteroids on the topical anti-inflammatory activity. Two different crystal forms, Form A and Form B, of the drugs of prednicarbate, hydrocortisone, betamethasone 17-valerate, prednisolone, and methyl prednisolone were prepared and their topical anti-inflammatory activities were measured using arachidonic acid induced ear edema assay in mice. Two crystal forms of the drugs showed differences in anti-inflammatory activity. Among the drugs examined, Form B of prednicarbate and betamethasone 17-valerate showed significantly more potent anti-inflammatory activities as compared to their Form A.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.97-100
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• 2015

The thermostable pectinase gene TmPec isolated from Thermotoga maritima was introduced into the NdeI site of pRSET-B vector and expressed in its intact form in Escherichia coli BL21. The overexpressed intact form of pectinase (TmPecN protein) was partially purified by heat-denaturation procedure. TmPecN showed the highest activity between 85 and $95^{\circ}C$, and at approximately pH 6.5. Enzyme activity was stably maintained at temperatures below $85^{\circ}C$. In the presence of $Ca^{2+}$, pectinase activity of TmPecN increased to 128.4% of normal level. In contrast, $Ba^{2+}$, $Zn^{2+}$, and $Mn^{2+}$ strongly inhibited TmPecN activity. We conclude that the biochemical properties of the intact form of TmPecN are comparable to those of the recombinant protein TmPec reported previously.

• Microbiology and Biotechnology Letters
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• v.21 no.3
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• pp.247-255
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• 1993

Antitumor activity of Lactobacillus casei YIP 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for supperssion of local tumor were examined. Administration of 100ng/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL.

• Korean Journal of Pharmacognosy
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• v.14 no.1
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• pp.17-23
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• 1983

To experimentally research out the effect of 'Hagocho-San' used for liver disease on the clinical treatment of oriental medicine, I induced liver damage rats by thioacetamide and dosed the extracts orally and measured the activity rate of GOT, GPT, ALP and LDH. The following results were obtained: 1) GOT activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was more efficacious than those mixed form of the extracts after individual decoction. Extracts from decoction showed continuous significance after 48 hours on, but mixed form of extracts after individual decoction was significant only after 48 hours and 96 hours. 2) GPT activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was effective after 48 hours on but mixed form of extracts after individual decoction was significant after 48 hours and 72 hours. 3) ALP activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was more efficacious than the mixed form of extracts after individual decoction and significance was verified after 72 hours on. 4) The significance of LDH activity in the extracts from mixed decoction was revealed after 72 hours and 96 hours but mixed form of extracts after individual decoction showed significance 72 hours later. 5) From the above results, it was showed that 'Hagocho-San' extract was effective on liver disease and extracts from mixed decoction was more effective than mixed form of extracts after individual decoction.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.2
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• pp.308-315
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• 2014

The aims of this study are to analysis about surface muscle activity of lower and upper musculus erector spinae according to walkers form. The subjects of the current step is inconvenient to use a walker and five people over the age of 65 valid samples were selected as final. All the subjects were measured in three forms. Wireless EMG muscle activity is a measure of surface measurements were measured using a system, both musculus vastus lateralis, outside the erector spinae muscle, musculus biceps femoris, gastrocnemius muscle was attached to the channel electrodes. According to the results, First, gastrocnemius muscle activity showed significant differences by type of gait. Second, the muscle activity of the erector spinae muscle walking showed significant differences by type, side grip type showed the lowest muscle activity. Consequently, the present study is the efficient use of the four-wheel walker will provide the baseline data.

• Archives of Pharmacal Research
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• v.17 no.5
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• pp.318-322
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• 1994

Upon gel filtration, the commercial bovine milk xanthine oxidase preparation was fractionated into two preparations showing enzyme activity. Native polyacrylamide gel electrophoresis showed that one was in a dimeric form and the other was a monomer having molecular weight of 150 kDa. It was also found that this commercial enzyme existed mostly in an active monomeric form without loss of enzyme activity. The rabbit antisera produced against two enzyme preparations cross-reacted well each other. In SDS-polyacrylamide gtel electro-phoresis, however, both enzyme preparations yielded two smaller protein bands below 150 kDa, which appeared to bind with both antisera with high affinity but not to retain enzyme activity. It implies that bovine milk xanthine oxidase can lose its activity when monomeric subunit is further degraded.

• Journal of the Korean Institute of Landscape Architecture
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• v.21 no.4
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• pp.43-53
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• 1994

This Study is to construct the Activity System Methods of Site Planning and Design. A planned spaces and facilities is considered usually in relation to users of space and facilities, user attributes, needs and values, and activity patterns. Information obtained form this study adds a qualitative dimension to the quantitative emphasis and supplies information for design-oriented analyses of site planning. In the design-oriented aspect, the concern is with devising desirable land use arrangements and facility supply considering objectives defined in the public interest. The design-oriented approache in site planning and design must use information on activity systems and space qualities in defining planning and design requirements of site development-one in the form of principles and standards of design used in land use and facility planning guidelines, and the other form of input variables for land use model and facility supply model.

• Physical Therapy Korea
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• v.21 no.4
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• pp.56-66
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• 2014

For maintaining adequate psychometric properties when reducing the number of items from an instrument, item level psychometrics is crucial. Strategies such as low item correlation or factor loadings, using classical test theory, have traditionally been advocated. The purpose of this study is to describe the development of a new short form assessing the impact of low back pain on physical activity. Rasch measurement model has been applied to the International Classification of Functioning, Disability and Health Activity Measure (ICF-AM). One hundred and one individuals with low back pain aged 19-89 years (mean age: $48.1{\pm}17.3$) who live in the community were participated in the study. Twenty-seven items of lifting/carrying construct of the ICF-AM were analyzed. Ten items were selected from the construct to create a short form. Item elimination criteria include: 1) high or low mean square (out of the range: .6-1.4 for the fit statistics), 2) similar item calibrations to adjacent items, 3) person separation value, and item-person map for potential gap in person ability continuum. All 10 items of the short form fit to the Rasch model except one item (i.e., carrying toddler on back). Despite its high infit and outfit statistics (1.90/2.17), the item had to be reinstated due to potential gaps at the upper extreme of person ability level. The short form had a slightly better spread of person ability continuum compared to the entire set of item. The created short form separated individuals with low back pain into nearly 4 groups, while the entire set of items separated the individuals into 6 groups. The findings prompted multidimensional models for better explanation of the lifting/carrying domain. The item level psychometrics based on the Rasch model can be useful in developing short forms with rationally retained items.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.507-521
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• 2014

A phase variation has been reported in an entomopathogenic bacterium, Xenorhabdus nematophila. Compared with a wild-type primary form, a secondary form usually loses several physiological and biochemical characters. This study showed that the phase variation of X. nematophila caused a significant alteration in its immunosuppressive activity and subsequent entomopathogenicity. A secondary form of X. nematophila was detected in laboratory colonies and exhibited significant differences in dye absorption and entomopathogenicity. In addition, the secondary form was different in its production of eicosanoid-biosynthesis inhibitors (EBIs) compared with the primary form of X. nematophila. Production of oxindole and p-hydroxypropionic acid was significantly reduced in the culture broth of the secondary form of X. nematophila. The reduced EBI production resulted in significant suppression in the inhibitory effects on cellular nodule formation and phenoloxidase activity. Culture broth of the primary form of X. nematophila enhanced the pathogenicity of Bacillus thuringiensis ( Bt) significantly more than the culture broth of the secondary form. Furthermore, this study developed a highly efficient "Dual Bt-Plus: to control both lepidopteran insect pests Plutella xylostella and Spodoptera exigua, by mixing two effective Bt strains along with the addition of potent bacterial metabolites or 100-fold concentrated X. nematophila culture broth.

• Korean Journal of Microbiology
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• v.8 no.2
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• pp.65-68
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• 1970

In continuation of the previous work (The New Medical Journal, Vol. 12, No. 3, 111, 1969), the effects on the bactericidal activity against coli form group, on vitamin C content and ascorbate oxidase activity of the purple laver due to the $^{80}Co$ gamma-irradiation were studied. The results obtained are ; 1) After the 0.1m rad./hr. doses treatment of gamma-irradiation for 1 hours to the laver, the coli form group was being remarkably destoryed and after the application for 10 hours the coli form group was completely destroyed. 2) The content of vitamin C was gradually decreased during the gamma-irradiation to the laver. According to the sensory test, no changes in flavor nad color were indicated for 9-10 hours treatment. But, the loss of ascorbic was much than that of dehydroascorbic acid after 10-hour treatment. 3) And also, the ascorbate oxidase activity due to the irradiation waas conspicuously decreased.