• 한국치위생학회지
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• 제10권5호
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• pp.887-895
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• 2010

Objectives : In this study, 233 dental hygiene students in Seoul and Gyeonggi areas were surveyed in order to provide the basic data for more effective clinical practice by investigating the stress they experience during the clinical practice and how they cope with stress; Methods : 5 areas in stress factors as environmental factor, interpersonal factor, role and activity, ideal and value, and the amount of BEPSI and 4 areas in their coping methods as problem-oriented, social support, emotion-oriented, and wishful thinking were measured by Liker 5-point scale. Results : 1. The satisfaction level of subjects on their major was the highest as 'generally satisfied (50.6%),' and their satisfaction level of clinical practice was also the highest as 'generally satisfied (32.6%).' 2. The clinical practice stress was 2.76 points on average, and their stress factors were ideal and value (3.18), environmental factor (2.98), role and activity (2,70), the amount of BEPSI (2.55), and interpersonal factor (2.37). And the way how they cope with stress were 3.29 points on average, wishful thinking (3.71), social support (3.36), problem-oriented (3.13), and emotion-oriented (2.95). 3. The clinical practice stress according to clinical training institutions showed significant differences: university dental hospitals and general hospitals (2.83), dental clinics (2.65), and dental hospitals (2.63). 4. The clinical practice stress showed a negative correlation between the satisfaction level of major and the satisfaction level of clinical practice (p<0.01), and the more satisfaction they had in their major and clinical practice, the less stress during the clinical practice. 5. In the correlation between each subordinate factor of the stress in clinical practice and how to cope with it, interpersonal factor and emotion-oriented factor, the amount of BEPSI and wishful thinking showed a positive correlation (p<0.05). Conclusions : In this study, it is necessary to develop clinical practice program that is to increase satisfaction and provide motivation in order to reduce the stress during clinical training.

• 대한물리치료과학회지
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• 제9권1호
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• pp.123-128
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• 2002

Objectives: The objective of this study was to investigate factor analysis of low back pain patients. Methods: The data were collected from hospital located in Daegu. Observation was made on 40 low back pain patients and factor analysis study was also performed in terms of their abnormal physical findings. Results: The low back pain patients occupied 20% of all clinical patients in male group and 20% in female group. The occurrence of acute low back pain in the first factor tended to be higher among in male group than among in female group. While it was significantly higher muscle weakness of the back in female group. The most prevailing abnormal finding among low back pain patients in the first factor were tender point on the back, While it was significantly lower Decreased tendon reflex in both sexes Conclusions: The factor pattern of abnormal physical findings in low back pain patients was much different between male and female patients, suggesting the different pattern of etiology. Although low back pain is one of the most common symptoms causing limitation activity, as yet there is no known specific effective treatment.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.293.2-293.2
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• 2002

Bisphenol A ［BPA. 2.2-bis(4-hydroxyphenyl)propane］ is reported to have estrogenic activity: however. its influence on cytokine production or immune system function remains unclear. In this study. we investigated the effects of BPA on the production of nitric oxide (NO) and tumor necrosis factor-a (TNF-a), and on the level of inducible nitric oxide synthase (iNOS) and TNF-a gene expression in mouse macrophages. BPA alone did not affect NO or TNF-a production. (omitted)

• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.37-44
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• 2017

Regulation of vascular smooth muscle cell (VSMC) phenotype plays an essential role in many cardiovascular diseases. In the present study, we provide evidence that $kr{\ddot{u}}ppel$-like factor 8 (KLF8) is essential for tumor necrosis factor ${\alpha}$ ($TNF{\alpha}$)-induced phenotypic conversion of VSMC obtained from thoracic aorta from 4-week-old SD rats. Stimulation of the contractile phenotype of VSMCs with $TNF{\alpha}$ significantly reduced the VSMC marker gene expression and KLF8. The gene expression of KLF8 was blocked by $TNF{\alpha}$ stimulation in an ERK-dependent manner. The promoter region of KLF8 contained putative Sp1, KLF4, and $NF{\kappa}B$ binding sites. Myocardin significantly enhanced the promoter activity of KLF4 and KLF8. The ectopic expression of KLF4 strongly enhanced the promoter activity of KLF8. Moreover, silencing of Akt1 significantly attenuated the promoter activity of KLF8; conversely, the overexpression of Akt1 significantly enhanced the promoter activity of KLF8. The promoter activity of SMA, $SM22{\alpha}$, and KLF8 was significantly elevated in the contractile phenotype of VSMCs. The ectopic expression of KLF8 markedly enhanced the expression of SMA and $SM22{\alpha}$ concomitant with morphological changes. The overexpression of KLF8 stimulated the promoter activity of SMA. Stimulation of VSMCs with $TNF{\alpha}$ enhanced the expression of KLF5, and the promoter activity of KLF5 was markedly suppressed by KLF8 ectopic expression. Finally, the overexpression of KLF5 suppressed the promoter activity of SMA and $SM22{\alpha}$, thereby reduced the contractility in response to the stimulation of angiotensin II. These results suggest that cross-regulation of KLF family of transcription factors plays an essential role in the VSMC phenotype.

• 디지털융복합연구
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• 제13권3호
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• pp.65-73
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• 2015

본 연구의 목적은 지속가능 PLM(Product Lifecycle Management) 활동과 성능에 관한 관계를 분석하고자 한다. PLM 활동의 효과를 알아보기 위해서 전통적인 PLM 활동과 지속가능 PLM 활동을 고려하여 S-PLM 프레임워크를 디자인 했다. 또한 제조기업의 PLM성공 요소를 추적하고 각 성공요소들간의 관계를 이해하기 위해 경로 분석을 실시하였다. 본연구의 결과는 4가지로 요약할 수 있다. 첫째, 제품정보, 제조정보 그리고 엔지니어링정보와는 지속가능 성과와 아무런 연관이 없다. 둘째, 지속가능 활동과 성과 간에는 양의 상관관계가 존재한다. 셋째, 전통적이 PLM활동과 지속가능 PLM활동 요소는 혁신성과요소를 포함하고 있다. 넷째, 지속가능 성과는 경영과 비즈니스 성과에 영향을 끼친다. 이에 본 연구에서는 지속가능 PLM의 구축 메카니즘과 활동 요소간의 영향도를 확인하였다. 본 연구의 기대효과로는 융복합적 PLM을 구축하고 운영하는데 혁신적인 성과가 창출되고 효율성이 나타나게 된다.

• 한국미생물·생명공학회지
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• 제44권2호
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• pp.117-123
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• 2016

본 연구에서는 C. pubescens Burkill 에탄올 추출물(CPEE)의 항산화능과 항염증 생리활성을 DPPH 라디칼 소거능, ROS 생성 억제능, NO 소거 활성 등을 통해 분석하였다. 먼저 CPEE의 항산화능을 DPPH 라디칼 소거능으로 분석한 결과 강한 소거능을 보였으며, 좀 더 자세한 항산화능 작용기작을 알아보기 위해 ROS 생성 억제능으로 분석한 결과 농도 의존적으로 강한 ROS 소거능을 보였다. 뿐만 아니라 대표적인 항산화 효소인 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현에 미치는 영향을 분석한 결과 CPEE에 의해 HO-1 및 Nrf2의 발현이 증가됨을 보였다. 한편 CPEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도의 존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 CPEE의 높은 항산화능과 항염증 활성을 확인하였으며 향후 잠재적인 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제22권6호
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• pp.679-688
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• 2018

Autism spectrum disorders (ASDs) are neurodevelopmental disorders that share behavioral features, the results of numerous studies have suggested that the underlying causes of ASDs are multifactorial. Behavioral and/or neurobiological analyses of ASDs have been performed extensively using a valid model of prenatal exposure to valproic acid (VPA). Abnormal synapse formation resulting from altered neurite outgrowth in neural progenitor cells (NPCs) during embryonic brain development has been observed in both the VPA model and ASD subjects. Although several mechanisms have been suggested, the actual mechanism underlying enhanced neurite outgrowth remains unclear. In this study, we found that VPA enhanced the expression of brain-derived neurotrophic factor (BDNF), particularly mature BDNF (mBDNF), through dual mechanisms. VPA increased the mRNA and protein expression of BDNF by suppressing the nuclear expression of methyl-CpG-binding protein 2 (MeCP2), which is a transcriptional repressor of BDNF. In addition, VPA promoted the expression and activity of the tissue plasminogen activator (tPA), which induces BDNF maturation through proteolytic cleavage. Trichostatin A and sodium butyrate also enhanced tPA activity, but tPA activity was not induced by valpromide, which is a VPA analog that does not induce histone acetylation, indicating that histone acetylation activity was required for tPA regulation. VPA-mediated regulation of BDNF, MeCP2, and tPA was not observed in astrocytes or neurons. Therefore, these results suggested that VPA-induced mBDNF upregulation was associated with the dysregulation of MeCP2 and tPA in developing cortical NPCs.

• 복식
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• 제25권
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• pp.233-247
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• 1995

The objectives of this study were to classify the contents of clothing involvement, to group elderly women into life style types. A method of this study was face to face re-search and questionnaire. Questionnaire was comprised of four sections : 18 Likert type items of clothing involvement measure ; 26 Likert type items of life style measure ; 3 items of clothing purchase measure ; and 3 demographic variables. Samples were 215 elderly women(60∼79 years of age) in Seoul, Korea. The data were analyzed using factor analy-sis, cluster analysis, one-way ANOVA, Dun-can's multiple range test,χ2 test. The results of the study were the follow-ings. 1. Four factors of clothing involvement derived by factor analysis : F.1 'clothing pleasure'; F.2 'clothing symbolism' ; F.3 'perceived risk in clothing purchase' ; F.4 'clothing interest'. 2. Four factors of life style derived by factor analysis : F.1 'active-leisure';F.2 'confidence oriented';F.3 'appearance interest';F.4 'house-work interest and community conciousness'. Three types of life style were defined by the cluster analysis of the 4 factors : T.1 'passive stag-nation'; T.3'outside activity'. 3. There were significant differences in clothing involvement factors according to life style types. Outside activity type perceived 'clothing pleasure' highest level among 3 life style types. Outside activity type and house-work and positive living type perceived 'cloth-ing symbolism' and 'clothing interest' higher level than did passive stagnation types. 4. Elderly women high in educational level were more distributed in outside activity type and the low in educational level in passive stagnation types. 5. There were significant relationships be-tween life style types and source of a clothing allowance, clothing purchase frequency, and a companion of dress store.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.291-295
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• 2010

The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors and individual host responses to these determinants, In both humans and mice, liver injury is involved after parasite entry, which persists until the erythrocyte stage after infection with the fatal strain Plasmodium falciparum (Pf), Hepatocyte growth factor (HGF) has strong anti-apoptotic effects in various kinds of cells, and also has diverse metabolic functions. In this work, Pf-subtilisin-like protease 2 (Pf-Sub2) 5' untranslated region (UTR) was analyzed and its transcriptional activity was estimated by luciferase expression. Fourteen TATA boxes were observed but only one Oct-1 and c-Myb were done. In addition, host HGF interaction with Pf-Sub2 was evaluated by co-transfection of HGF- and Pf-Sub2-cloned vector. Interestingly, -1,422/+12 UTR exhibited the strongest luciferase activity but -329 to + 12 UTR did not exhibit luciferase activity. Moreover, as compared with the control of unexpressed HGF, the HGF protein suppressed luciferase expression driven by the 5' untranslated region of the Pf-Sub2 promoter. Taken together, it is suggested that HGF controls and interacts with the promoter region of the Pf-Sub2 gene.

• 한국콘텐츠학회논문지
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• 제16권1호
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• pp.642-654
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• 2016

이 연구는 초등학생의 자기관리의 특성을 분석하고 신체활동 자기관리의 검사지를 개발하는데 목적이 있다. 이를 위해 일반학생에서부터 체육영재로 선발되어 훈련받고 있는 학생, 그리고 현재 각급 학교에서 운동선수로 활동하고 있는 어린이들까지 망라하여 총 1005명의 다양한 학생들이 참여하였다. 단계별로 전문가 회의, 귀납적 범주화, 문항분석, 탐색적 요인분석, 확인적 요인분석을 실시하고, 변별타당도 검증을 위해 집단 간 차이검증을 실시하였다. 그 결과, 신뢰도와 타당도 검증을 거쳐 초등학생의 신체활동 자기관리 요인은 운동관리, 생활관리, 재미, 자신감, 부모의 지지, 대인관계 6요인 총 24문항으로 확정되었다. 또한, 변별 타당도를 검증하기 위해 일반 어린이와 체육 영재 어린이의 집단 간 신체활동 자기관리를 검증한 결과 운동관리, 대인관리, 재미, 자신감, 부모의 지지, 대인관계의 모든 변인에서 유의한 차이가 나타났으며, 체육영재 어린이가 일반 어린이 집단에서 보다 신체활동 자기관리의 모든 하위요인에서 더 높게 나타났다. 결과적으로, 초등학교 현장에서 신체활동 자기관리를 진단하는 검사지로서의 타당성을 확보하였다.