• 제목/요약/키워드: Activity Determination

검색결과 773건 처리시간 0.048초

Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

  • Kim, Dongwook;Kim, Hee-Jin;Chae, Hyun-Seok;Park, Nam-Gun;Kim, Young-Boong;Jang, Aera
    • 한국축산식품학회지
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    • 제34권6호
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    • pp.844-851
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    • 2014
  • This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was $364.72{\mu}M/TE$ and ORAC activity (1 mg/mL) was $101.85{\mu}M/TE$. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of $100mJ/cm^2$. After 24 h of incubation with $500{\mu}g/mL$ LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.

청도반시 추출물에 의한 라디칼소거 활성과 면역조절 활성의 상호 비교 (Comparison of Radical Scavenging and Immunomodulatory Activities Exhibited by an Aqueous Extract of Diospyros kaki Thunb. Fruit (Persimmon))

  • 허진철;채장희;이숙희;이윤래;문광덕;정신교;이상경
    • 한국식품저장유통학회지
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    • 제15권5호
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    • pp.749-753
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    • 2008
  • 청도반시 추출물로부터 라디칼 소거활성, T-bet promoter 활성, IL-4 발현 활성의 상관관계를 분석하고자 7월부터 10월까지 감을 시기별로 수확하여 분자염증 활성 여부를 확인하였다. 감의 무게는 시기가 경과할수록 무거워지면서 항산화 활성에 있어서는 감이 미숙과일수록 항산화활성이 우수하였다. 4가지의 용매로 추출한 감의 항산화 활성도 유사한 양상을 보였다. T-bet promoter 활성은 추숙이 될 수록 억제되는 양상을 보였는데, 이에 반하여 IL-4의 발현 정도는 숙성함에 따라 증가하는 것을 알 수 있었다. 이로 미루어 보아 수확시기에 따라 이러한 분자염증에 관련한 일련의 활성의 억제 효과는 감이 미숙과일수록 활성이 상승하였다.

Comparison of Biological Activities of Dendropanax morbiferus by Different Cultivation Areas in Korea

  • Oh, Junseok;Hong, Jae-Heoi;Park, Tae-Young;Jeon, Byeonggyu;Ha, Neul-I;Kim, Kyung Je;Jin, Seong Woo;Koh, Young Woo;Im, Seung Bin;Jeong, Hee Gyeong;Seo, Kyoungsun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.105-105
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    • 2019
  • Dendropanax morbifera H.Lev is an evergreen tree that lives in subtropical climates. About 75 species of D. morbifera live in East Asia, but only one species live in Korea(Korean dendropanax). D. morbifera belonging to Aralicacae family 98% of D. morbifera are distributed in Jeollanam-do in the Korea and the grow wild in Gyeongsangnam-do and Jeju Island. The harvest time and usage of D. morbifera were recorded in traditional medicinal books. The roots and stems of D. morbifera had been used for traditional medicine to treat migraine, menstrual irregularity and skin disease. And D. morbifera leaves are contain flavonoids and polyacetylene compounds. In this study, we were investigated the physiological activity of D. morbifera by different areas collected at the same time, and compared to characteristics of plants. D. morbifera collected from Jeollanam-do (Goheung-gun), Gyeongsangnam-do (Namhae-gun) and Jeju Island, and dried at $50^{\circ}C$ for three days. We used dried D. morbifera powder for antioxidant tests. Each sample was extracted with hot water under the same conditions. The contents of total polyphenols and total flavonoids from D. morbifera were identified. Also, we performed to DPPH radical scavenging activity, ABTS cation radical scavenging activity and Superoxide anion scavenging activity efficacy for antioxidant activity determination. The contents of total polyphenols and total flavonoids in hot water extract of D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do were higher than Jeju. However, D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do showed no significant difference those content of total polyphenols and total flavonoids. And the antioxidant capacity was showed the similar patterns in antioxidant activity.

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A Study on the Radiometric Method for the Determination of Uranium and Thorium in Ores

  • Lee, Chul;Lee, Keun-Min
    • Nuclear Engineering and Technology
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    • 제12권2호
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    • pp.106-110
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    • 1980
  • 옥천계 흑혈암(black shale) 및 모나자이트에 함유된 우라늄 및 토륨의 방사화 평형조건에 관하여 연구하였다. 이 연구의 결과로 감마선 계측에 의한 이들 원소의 분석법을제시할 수 있었으며 많은 수의 광물 시료분석이 손쉽게 되었다.

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Studies on the Venom Inhibtor (Part Ⅵ) Reaction of the sample in vivo

  • 서정훈
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 1978년도 추계학술대회
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    • pp.209.4-209
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    • 1978
  • Previously, we reported the inhibitory substane which reacts on venoms proteinases and haemorrha-ghagic factors. The active substsnce was originated from soil fungi. This report describes the results of molecular weight determination, the activity by the derivatives, and also the reaction in vivo by the administration of sample L175-68-B.

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청각 감성의 생리적 신호변화에 대한 연구

  • 황민철;김지은;김철중
    • 대한인간공학회:학술대회논문집
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    • 대한인간공학회 1996년도 춘계학술대회논문집
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    • pp.259-263
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    • 1996
  • Psychological action is physiological response of outernal stimulus. Physiological response is accompanied b physiological signals which are EEG, EMG, GSR, ECG, BP, and tec. Physiological signals are recently studied for determination of human phychological state. Psychological activity causes electric potential of brain. Physiological signal is considered as measurement of human psychological state. Aditory sensibility which is one of the sense of human may determine differences between positive and negative feeling. EEG and GSR variation with auditory quality of stimulus can be define human negative and positive mental state. This study is to characterize parameters which can determine negative and positive psycholigical state of human.

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