This study was a cross-sectional descriptive investigation study that analyzed the effects of spiritual well-being on stress coping in twenties. Data collection period was from May 1, 2019 to May 20, and 207 people voluntarily participated in the study. The sub-factors of spiritual well-being consisted of religious well-being and existential well-being. The types of stress coping consist of active coping, passive coping, aggressive coping, evasive coping, and social supportive coping. Data was analyzed using frequency analysis, t-test, Pearson correlation and multiple regression analysis using SPSS 18.0. As a result of the analysis, 90 (43.5%) were male and 117 (56.5%) were female. The 71.0% of the participants had no religion, the Buddhists were 14.5%, other religions were 5.8%, Protestants were 4.3%, Catholics were 2.9%, and Won Buddhists were 1.4%. Active coping in stress coping was correlated with religious well-being (r=-.357, p<0.01) and existential well-being (r=.301, p<0.01) under statistical significance. Religious well-being was significantly higher in males (Mean=2.87, SD=.753) than females (Mean=2.49, SD=.772, p<0.01). Existential well-being was also higher in males (Mean=4.25, SD=.841) than in females (Mean=3.95, SD=.694, p<0.01). The religious well-being was significantly higher than active coping (t=6.183, p<0.01), passive coping (t=-3.595, p<0.01), aggressive coping (t=1.991, p<0.05). Existential well-being was significantly higher than active coping (t=5.339, p<0.01), aggressive coping (t=1.659, p<0.05), evasive coping (t=-3.709, p<0.05) at the statistical significance level. Based on the results, it can be suggested that it was necessary to systematically plan spiritual well-being program as a way to cope with stress by knowing that spiritual well-being positively affects active, aggressive stress coping.