• Korean Journal of Pharmacognosy
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• 제35권4호통권139호
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• pp.364-367
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• 2004

Cordyceps militaris (CM) has been used as antidiabetics, anticancer, endocrine and sexual functions enhancement in the traditional medicine. Water soluble fractions of CM showed cytotoxic activities on the three kinds of human cancer cell lines, stomachic adenocarcinoma(SNU-1), colorectal adenocarcinoma (SNU-C4), and hepatocellular carcinoma(SNH-354). Cytotoxic activity guided isolation and identification of active fractions afforded cordycepin as an active component.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 춘계학술대회
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• pp.208-211
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• 2007

Polymer Electrolyte Membrane fuel cell(PEMFC)의 촉매로는 주로 carbon을 지지체로 사용한 Pt/C 흑은 Pt alloy/C을 사용하게 된다. 이때 PEMFC의 상용화에 있어 촉매의 안정성은 상용화 실현에 있어 중요한 요인으로 인식되고 있다. 촉매의 성능 저하는 Pt의 활성 면적(Active surface area)의 감소가 원인으로 얘기되어 지고 있으며 이는 지지체로 사용한 탄소에 나노 크기로 분산되어 있던 Pt 입자가 커지면서 활성 면적이 감소되어 지기 때문이다. 이번 연구에서는 상용 Pt/C를 사용하여 Cyclic Voltammetry(CV)의 장기간 운전 및 다양한 조건 변화를 통하여 Pt입자의 크기 증가에 미치는 요인에 대한 연구를 진행하였다. 이와 더불어 Linear Sweep Voltammety(LSV), TEM, 등을 통한 분석이 진행되었다.

• Journal of Plant Biology
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• 제33권2호
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• pp.87-96
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• 1990

The role of S-adenosylmethionine (SAM) as an intermediate in interrelation between polyamine and ethylene biosynthesis was studied in suspension cultures of Nicotiana tabacum L. Exogenous SAM stimulated the polyamine and ethylene biosynthesis in 4 day-cultured cells, which were in active cell divisions, and 10 day cultured cells, which went on with active cell elongation and senescence. SAM-induced ethylene production was more effective in 10 day-cultured cells than in 4 day-cultured cells, but SAM-induced polyamine biosynthesis was more effective in 4 day-cultured cells than in 10 day-cultured cells. Polyamine contents were increased by the blockage of ethylene biosynthetic pathway in the conversion of SAM to ethylene via 1-aminocyclopropane-1-carboxylinc acid (ACC) with aminooxyacetic acid (AOA). Also, ethylene production was increased by the inhibitors of polyamine biosynthesis such as methylglyoxal bis-(guanylhydrazone) (MGBG), dicyclohexylamine (DCHA), $\alpha$-difluoromethylarginine (DFMA) and $\alpha$-difluoromethylorinithine (DFMO). These results suggest that there may be interrelations between polyamine and ethylene biosynthesis for the competition of SAM and the inherent mechanism of switch on-off in polyamine and ethylene biosynthetic activity with the progress of cell growth and senescence.

• Journal of Power Electronics
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• 제10권3호
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• pp.235-244
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• 2010

This paper presents a new isolated soft-switching bidirectional buck-boost inverter for fuel cell applications. The buck-boost inverter combines an isolated DC-DC converter with a conventional inverter to implement buck-boost DC-DC and DC-AC conversion. The main switches achieve zero voltage switching and zero current switching by using a novel synchronous switching SVPWM and the volume of the transformer in the forward and fly-back mode is also minimized. This inverter is suitable for wide input voltage applications due to its high efficiency under all conditions. An active clamping circuit reduces the switch's spike voltage and regenerates the energy stored in the leakage inductance of the transformer; therefore, the overall efficiency is improved. This paper presents the operating principle, a theoretical analysis and design guidelines. Simulation and experimental results have validated the characteristics of the buck-boost inverter.

• The Korean Journal of Physiology and Pharmacology
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• 제7권1호
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• pp.33-37
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• 2003

Oxidative damage to mitochondria is a critical mechanism in necrotic or apoptotic cell death induced by many kinds of toxic chemicals. Thioredoxin (Trx) family proteins are known to play protective roles in organisms under oxidative stress through redox reaction by using reducing equivalents of cysteines at a conserved active site, Cys-X-X-Cys. Whereas biological and physiological properties of Trx1 are well characterized, significance of mitochondrial thioredoxin (Trx2) is not well known. Therefore, we addressed physiological role of Trx2 in PC12 cells under oxidative stress. In PC12 cells, transiently overexpressed Trx2 significantly reduced cell death induced by hydrogen peroxide, whereas mutant Trx2, having serine residues instead of two cysteine residues at the active site did not. In addition, stably expressed Trx2 protected PC12 cells from serum deprivation. These results suggest that Trx2 may play defensive roles in PC12 cells by reducing oxidative stress to mitochondria.

• Preventive Nutrition and Food Science
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• 제14권2호
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• pp.162-167
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• 2009

Various fermented foods were screened in search of food-grade bacteria that produce bacteriocins active against Gram-negative pathogens. An isolate from a mold-ripened cheese presented antibacterial activity against Gram-positive and Gram-negative bacteria. The most active isolate was identified as Lactobacillus casei by a biochemical method, ribotyping, and membrane lipid analysis, and was designated as OSY-LB6A. The cell extracts of the isolate showed inhibition against Escherichia coli p220, E. coli O157, Salmonella enerica serovar Enteritidis, Salmonella Typhimurium, and Listeria monocytogenes. The antibacterial nature of the cell extract from the isolate was confirmed by eliminating the inhibitory effects of acid, hydrogen peroxide, and lytic bacteriophages. The culture supernatant and cell extract retained antibacterial activity after heating at $60{\sim}100^{\circ}C$ for $10{\sim}20$ min. The activity of the cell extract from Lb. casei was eliminated by pronase and lipase. Finally, the cell extract showed a bactericidal mode of action against E. coli in phosphate buffer solution, but it was bacteriostatic in broth medium and food extracts.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.146.1-146.1
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• 2010

최근 전력 수요의 증가와 지구 온난화 문제가 화두가 되면서 피크 전력관리와 스마트 그리드의 필요성이 강조되고 있다. 이들을 구현하기위해 반드시 필요한 것이 에너지 저장 시스템이다. 본 발표는 배터리 에너지 저장 시스템에 사용되는 능동형 셀 발란싱 기능을 갖는 배터리 관리 시스템에 관한 것이다. 전력 에너지 저장용 배터리는 원하는 전압과 전력을 저장하기위해 단전지(Cell)들을 보통 수천 개가 직렬 병렬로 연결되어 구성된다. 배터리팩을 구성하는 단전지 한 개의 용량이 다른 단전지 보다 크거나 작던지 또는 한 개의 단전지에 문제가 생기면 배터리팩 전체의 성능이 저하되든가 또는 사용 할 수가 없게 된다. 따라서 배터리팩을 구성 할 때는 용량이 동일 한 단전지들을 사용한다. 에너지 저장용 배터리팩에는 수백 와트의 단전지 들이 사용되므로 에너지 저장용 배터리팩을 위한 단전지 생산에는 많은 어려움이 있다. 능동형 셀 발란싱 기술을 사용 하면 이러한 배터리팩의 문제를 해결 할 수 있어 셀 제조원가를 절감 할 수 있을 뿐만 아니라 배터리팩의 가용용량을 늘릴 수 있고 또한 배터리팩의 수명을 연장 할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.414-421
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• 1999

Helicobacter pylori were found to be resistant to azide but sensitive to vanadate, suggesting that defect in the P-type ATPase activity rather than F-type ATPase would be lethal to cell survival or growth. To elucidate the relationship between this enzyme inhibition and H. pylori death, we determined the effect of omeprazole (OMP) plus vanadate on enzyme activity and cell growth. The minimum inhibitory concentration (MIC; ca. 0.8$\mu$mol/disk) of vanadate for H. pylori growth was lowered over l0-fold with the aid of OMP, whereby its inhibitory potential toward the P-type ATPase activity was diametrically increased. Alternatively, we found that this enzyme activity was essential for active transport in H. pylori. From these observations, we strongly suggest that the immediate cause of the growth inhibition of H. pylori cells with OMP and/or vanadate might be defective in the cell's active transport due to the lack of P-type ATPase activity. From the spectral data with circular dichroism (CD) spectroscopy, we found that activated OMP (OAS) at concentration below MIC did not disrupt helical structures of membrane proteins. Separately, we determined the cytopathic effect of OAS by SDS-PAGE, indicating the change in the production of cytoplasmic protein but not cell membrane.

• Advances in Traditional Medicine
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• 제3권4호
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• pp.187-190
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• 2003

Seven essential oils were tested for in vitro cytotoxicity against the cancerous cell lines A-549, HEp-2 and DLA and normal BRL-3A, NRK-49F and Vero cell lines using standard MTT, SRB and dye exclusion techniques. The A-549 cell line was found to be the most susceptible to all the essential oils. The essential oils of A. nilagirica, A. calamus and O. sanctum were found to be the more active against these cells with mean $CTC_{50}$ values of 17.75, 19.00 and $24.37\;{\mu}g/ml$, respectively. The essential oil of Acorus calamus was found to be the most potent with low $CTC_{50}$ values against the cancerous and comparatively higher $CTC_{50}$ values against the normal cell lines. Artemisia pellens and Pelargonium graveolens oils also showed potent activity. These oils merit further investigation to identify the active principles and nature of the anti tumor activity in animal models.

• The Transactions of the Korean Institute of Power Electronics
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• 제20권4호
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• pp.321-329
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• 2015

The static synchronous compensator (STATCOM) of cascaded H-bridge configuration accompanying multiple separate DC sides is inherently subject to the problem of uneven DC voltages. These DC voltages in one leg can be controlled by adjusting the AC-side output voltage of each cell inverter, which is proportional to the active power. However, when the phase current is extremely small, large AC-side voltage is required to generate the active power to balance the cell voltages. In this study, an alternative zero-sequence current injection method is proposed, which facilitates effective cell balancing controllers at no load, and has no effect on the power grid because the injected zero sequence current only flows within the STATCOM delta circuit. The performance of the proposed method is verified through simulation and experiments.