• 제목/요약/키워드: Active Application

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고등학교 천체 관측 동아리를 위한 방과 후 학교 프로그램 개발 및 적용: 관측지 주변 환경을 고려한 표준화 계수 결정 프로그램 (Development and Application of an After-school Program for an Astronomy Observation Club in a Highschool: Standardized Coefficient Decision Program in Consideration of the Observation Site's Environment)

  • 김승환;이효녕;이현동;정재화
    • 한국지구과학회지
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    • 제29권6호
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    • pp.495-505
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    • 2008
  • 이 연구의 목적은 방과 후 학교 활동으로 직접 활용될 수 있는 고등학교 천체관측반을 대상으로 한 천체 관측 프로그램 개발 및 적용이다. 연구방법은 먼저 현행 지구과학교과서의 천문관련 탐구활동을 분석한 다음, 가장 기본적인 관측 장비의 표준화 프로그램을 개발하였으며, 프로그램을 이용하여 학교 인근 여러 관측지에서 학생들이 직접 탐구활동을 수행하였다. 특히 관측 장비의 경우 일선학교에서도 쉽게 구비할 수 있는 102mm급 굴절망원경과 디지털카메라를 이용하였으며, 대부분 도심지에 위치한 학교의 관측지 환경을 고려한 탐구활동 및 그 수행을 위한 컴퓨터 프로그램을 개발하였다. 이 연구의 결과는 다음과 같다. 첫째, 현행 교육과정상의 천문관련 탐구활동은 대부분 제시된 자료를 분석하는 자료 해석이나 가상실험활동으로 구성되어 있다. 둘째, 대부분의 학교가 위치한 도심지의 경우 도시 외곽보다 관측 환경이 열악하다. 셋째, 학교 인근 도심지에서 보편화된 관측 장비를 이용한 효율적인 관측 기법과 도심지에서의 관측 기기의 표준화 계수를 결정하였다. 넷째, 이와 같은 일련의 과정을 프로그램화하여 측정한 결과, 도시와 도시외곽에서 실제 별의 등급과 큰 차이를 보이지 않았으며, 학습 프로그램 개발을 통하여 천체 관측에 관한 다양한 탐구 활동을 제공할 수 있었다 현재 천체 관련 교육은 실제 탐구 활동 영역이 결여되어 있으며, 대부분의 학교가 도심지에 위치하고 있어서 관측환경 또한 좋지 못하다. 하지만 이 프로그램을 이용하면 실질적인 천체관측 활동과 관측 결과에 대해 정형화된 보정활동을 거쳐 유의미한 관측 데이터를 수집할 수 있으며, 이를 여러 가지 활동을 통해 재해석 할 수 있다. 이를 통해 현장 체험 중심의 천체 탐구 활동을 활성화시킬 수 있으며, 과학적 흥미를 높일 수 있다.

Low temperature plasma deposition of microcrystalline silicon thin films for active matrix displays: opportunities and challenges

  • Cabarrocas, Pere Roca I;Abramov, Alexey;Pham, Nans;Djeridane, Yassine;Moustapha, Oumkelthoum;Bonnassieux, Yvan;Girotra, Kunal;Chen, Hong;Park, Seung-Kyu;Park, Kyong-Tae;Huh, Jong-Moo;Choi, Joon-Hoo;Kim, Chi-Woo;Lee, Jin-Seok;Souk, Jun-H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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    • pp.107-108
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    • 2008
  • The spectacular development of AMLCDs, been made possible by a-Si:H technology, still faces two major drawbacks due to the intrinsic structure of a-Si:H, namely a low mobility and most important a shift of the transfer characteristics of the TFTs when submitted to bias stress. This has lead to strong research in the crystallization of a-Si:H films by laser and furnace annealing to produce polycrystalline silicon TFTs. While these devices show improved mobility and stability, they suffer from uniformity over large areas and increased cost. In the last decade we have focused on microcrystalline silicon (${\mu}c$-Si:H) for bottom gate TFTs, which can hopefully meet all the requirements for mass production of large area AMOLED displays [1,2]. In this presentation we will focus on the transfer of a deposition process based on the use of $SiF_4$-Ar-$H_2$ mixtures from a small area research laboratory reactor into an industrial gen 1 AKT reactor. We will first discuss on the optimization of the process conditions leading to fully crystallized films without any amorphous incubation layer, suitable for bottom gate TFTS, as well as on the use of plasma diagnostics to increase the deposition rate up to 0.5 nm/s [3]. The use of silicon nanocrystals appears as an elegant way to circumvent the opposite requirements of a high deposition rate and a fully crystallized interface [4]. The optimized process conditions are transferred to large area substrates in an industrial environment, on which some process adjustment was required to reproduce the material properties achieved in the laboratory scale reactor. For optimized process conditions, the homogeneity of the optical and electronic properties of the ${\mu}c$-Si:H films deposited on $300{\times}400\;mm$ substrates was checked by a set of complementary techniques. Spectroscopic ellipsometry, Raman spectroscopy, dark conductivity, time resolved microwave conductivity and hydrogen evolution measurements allowed demonstrating an excellent homogeneity in the structure and transport properties of the films. On the basis of these results, optimized process conditions were applied to TFTs, for which both bottom gate and top gate structures were studied aiming to achieve characteristics suitable for driving AMOLED displays. Results on the homogeneity of the TFT characteristics over the large area substrates and stability will be presented, as well as their application as a backplane for an AMOLED display.

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초등학생의 또래 칭찬활동 프로그램이 자아존중감 및 대인관계에 미치는 효과 (A Study on the effect of Friend-praise activity program of Elementary students for Self-esteem and Personal Relationship)

  • 오은주
    • 초등상담연구
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    • 제5권1호
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    • pp.159-184
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    • 2006
  • Lately, the rapid social and family system change, the entrance examination-centered circumstance in education have a negative influence not only on making up the affirmative self-esteem but also on building up the personal relationship through mutual communication with friends of the same age. Making children adapt well to school life and develop a good relationship in the class is an important subject in educational field. Thus, various school activities should offer lots of opportunities to increase their affirmative self-esteem and more specified performance-centered programs are required toform a harmonious relationship among parties of students. The purpose of this study is to improve an affirmative self-esteern and a desirable personal relationship of children through developing friend-praise program and adapting it to children. Based on the above purpose, the following subjects for this study 'are suggested. Firstly, is the friend-praise program effective in forming an affirmative self-esteem of elementary students? Secondly, is the friend-praise program successful in improving a desirable personal relationship in an elementary school? In addition, two hypotheses of study are decided on the basis of theoretical background and the former study. Hypothesis 1. The friend-praise program will have a meaningful effect on improving an affirmative self-esteem. Hypothesis 2. The friend-praise program will have a meaningful effect on improving a desirable personal relationship. In order to improve the above hypotheses, the experiment was carried out with two classes on the fourth grade in M elementary school in Wonju with being divided into one experimental group and the other comparative group. The friend-praise program which was adapted to the experimental group was composed with four steps as 'introduction', 'preparation', 'operation' and 'closing'. This program which has twelve categories was executed once or twice in a week and each class was continued for forty or sixty minutes. Contrastively, comparative group had no application of the main program. For the purpose of proving the hypotheses after adapting the program to students, I have executed both self-esteem test and personal relationship test. The tests were performed with using SPSS/Windows V10.0 and the statistic was conducted through independant sample t-test for the difference between groups and contrastive sample t-test for the individual difference in each group. The results of this study can be summarized like the followings: Firstly, from the result of previous and after tests, there was no meaningful change in the comparative group, but the experimental group showed meaningful difference in all categories of self-esteem(t=-4.496, p=.000). Especially, the general self-esteem(t=-3.216, p=.003), social self-esteem(t=-2.680, p=.012), homely self-esteem(t=-3.732, p=.001), and school self-esteem(t=-3.902, p=.000) showed a meaningful difference. At the same time, the experimental group also showed meaningful difference with the comparative group in self-esteem(t=-4.758, p=.000). Especially, the general self-esteem(t=2.581, p=.017), social self-esteem(t=3.160, p=.003), homely self-esteem(t=4.283, p=.000), and school self-esteem(t=4.110, p=.000) showed a meaningful difference. As a result of this experiment, the friend-praise program will have a meaningful effect on improving an affirmative self-esteem was proved. Secondly, there was no meaningful change in the comparative group, but the experimental group showed meaningful difference in all categories of personal relationship(t=-4.131, p=.000). Specifically, satisfaction(t=-2.113, p=.045), communication(t=-3.381, p=.002), confidence(t=-3.517, p=.001), intimacy(t=-3.958, p=.000), sensibility(t=-2.955, p=.006), openness(t=-4.318, p=.000) and interest(t=-2.941, p=.000) showed a meaningful difference. In the same instant, the experimental group also showed meaningful difference with the comparative group in all categories of personal relationship(t=3.897, p=.000). Especially, satisfaction(t=2.257, p=.003), communication(t=3.527, p=.001), confidence(t=3.704, p=.001), intimacy(t=3.904, p=.000), sensibility (t=4.382, p=.000), openness(t=2.648, p=.013) and interest(t=2.944, p=.006) showed a meaningful difference. Accordingly, the friend-praise program will have a meaningful effect on improving a desirable personal relationship was proved. Judging from all these results, we should provide primary students with various opportunities to take part in active programs which help them to set up their righteous sense of value, to solve their own problems and to develop their potentials. Consequently, many kinds of practice-centered program like the friend-praise program should be developed more systematically and teachers should apply all those programs to students according to their individual level and developmental stage.

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희토류 자석의 자성이 골모세포 성장인자 수용체의 증가에 미치는 영향에 관한 연구 (Effect of the magnetism(neodymium magnet) on growth factor receptors of osteoblasts)

  • 이상민;이성복;최부병
    • 구강회복응용과학지
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    • 제19권2호
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    • pp.87-96
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    • 2003
  • The purposes of this study were to find out the optimum intensity of magnetic field where magnetism could promote the activity of osteoblast, and to discover the possibility of clinical application in the areas of dental implants and bone grafts by confirming the effect of clinically increasing bone formation. In this experiment, we used the Neodymium magnet, which had magnetic power six times as strong as the current ones and enabled the resistances against the demagnetization up to 20 to 50 times to be minimized with the size of 1mm in sight. In order to culture cells, a specially designed device was used. It was made to adjust the distance and accordingly to control the intensity of the magnetic field, by placing the cell culture plate in the center with a magnet of 1mm long and thick installed on the both ends. Using MC3T3-E1 cell, a kind of osteoblast-like cell, we cultured, for 24 hours, not only the test group which had been cultured under the magnetic fields with different intensity of 5, 10, 50, 100, 500, and 1000 Gauss, but also the control group excluding the influences of the magnetic field. After observing the cell's form and the density of the culture medium through an inverted microscope, we made a series of proceedings needed for the immunofluoroscence staining, such as fixation, normal serum reaction, primary antibody reaction, and secondary antibody reaction. And with a fluorescence microscope, we observed those-above and compared the frequency of expression of IFG-1 receptor. To make a Western immunoblotting analysis, the cells cultured under the same condition as the above had the procedure of the lysis buffer and the acrylamide gel electrophoresis was carried out. Protein transferred into the nitrocellulose membrane and tested on the primary and the secondary antibody reactions was observed and compared. The results were as follows: When observed through an inverted microscope, the nuclear divisions of the cells under the magnetic field of 10 Gauss were the most active, and the density of the cells could be observed the most enormously. As the result of an immunofluoroscence staining of IGF-1 receptor, the expression of IFG-1 was the most frequently observed under the magnetic field of 10 Gauss. On the other hand, few differences of consideration were made between the test group cultured under the magnetic fields of 5, 500, and 1000 Gauss and the control group. In respect of the expression of IFG-1 receptor, the test group cultured under the magnetic fields of 50 and 100 Gauss were higher than the control group, and lower than that cultured under the magnetic field of 10 Gauss.(p<0.05) According to the Western immunoblotting analysis, the band of IFG-1 receptor which had 85KDa of molecular weight was the darkest. Judging from the above-mentioned results, the growth factor receptor of an osteoblast cell which was an important criterion for the bone formation was increased in maximum under the magnetic field of 10 Gauss. Moreover it was observed that the optimum intensity of magnetic field in which magnetism made the activity of the osteoblast cell increase was about 10 Gauss.

1920년대 소비에트 구성주의 패션에 관한 연구 (A Study on Soviet Constructive Fashion in 1920s)

  • 조윤경;금기숙
    • 복식
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    • 제36권
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    • pp.183-203
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    • 1998
  • The wave of Avant-garde swept away all in the unique social background so called 'October Revolution' and the early 1900 Russian society which was able to absorb and accept anything. The Russian avant-garde has been affected by the Cubism and the Futurism those had peculiarly appeared in the early twentieth century, spreaded out to three spheres: the Suprematism, the Rayonism and the Constructivism. The Russian Constructivism has appeared in this background, concretely and ideally ex-pressed the ideology of the revolution into the artistic form and made an huge influence to the whole Russian society. The Constructivist like Tatlin, naum Gabo, Pevaner, Rodchenko, Stepanova, Popova and Exter gave great effect on the Soviet Constructive fashion design in 1920's after the Revolution. The Soviet costume in 1920s hold in common the characteristicss of the Constructive graphic as it is, geometrical and abstractive form, energetic and motility. In fashion design, these graphic qualities have been showed as the application of geometrical form and architectural image, physical distortion and transformation. And in textile design, the simple, dynamical presentation has been appeared. We can classify the Soviet costume at this time into three occasions. The first term is from late 1910 th mid 1920, and it is altered from folk costume design to modern one. With Lamanova as the first on the list, using the folk mitif, the Constructive expression of simple form has been gradually revealed in design. Designers like Makarova, Pribylskaia and Mukhina produced the plane, simple chemise style with the decoration of the Russian traditional motif. From early to late 1920 is the second term, and it is at the pick of the most active processing of the Constructive design. Not only at the costume in daily life but also at the theatrical costume and textile, the con-structive design has been represented all avail-able fields. Many Constructivists including Stepanova, Popova, Exter and Rodchenko took part in the textile design and costume design so as to evlvo their aesthetic concept. The third term is from late 1920 to early 1930. The socialistic realism has dominated over the whole culture and art, the revolutionary dynamic motif has been presented also in textile design. The formative features of Soviet Constructive fashion design are; silhouette, from, motif, color and fabric. The first, the silhouette : a straight rectangular silhouetted has been expressed through the whole period and a volumed one with distorted human body shape has introduced in the theatrical costume design. The second, the form: many lengthened rectangular forms have been made at beginnings, but to the middle period, geometrical, architectural forms have been more showed and there are energy and movement in design. At the last period, only a partial feature-division has been seen. The third, the motif; no pattern or ethnic motif has been partly used at beginnings, a figure like circle, tri-angle has gradually appeared in textile design. At latter period, a real-existent motif like an airplane has been represented with graphing and simplicity. The fourth, the color ; because of insufficient dyeing, neutral color like black or grey color has been mainly covered, but after middle term, a primary color or pastel tone has been seen, contrast of the fabric; without much development of textile industry after the Revolution, thick and durable fabrics have been the main stream, but as time had going to the last period, fabrics such as linen, cotton, velvet and silk have been varously choesn. At the theatrical costume, new materials like plastics and metals that were able to accentuate the form. The pursuit of popularity, simplicity and functionalism that the basic concept of Constructive fashion is one of the "beauty" which has been searching in modern fashion. And now we can appreciate how innovative and epochal this Soviet Constructive fashion movement was.ement was.

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STEAM 교육의 한계와 개선방향 -STEAM 교육 전문성을 가진 교사의 견해를 바탕으로- (Limits of STEAM Education and its Improvement Alternative : Based on the Viewpoints of STEAM Expert Teachers)

  • 손미현;정대홍
    • 한국과학교육학회지
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    • 제39권5호
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    • pp.573-584
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    • 2019
  • 2011년부터 시도된 STEAM 교육은 여전히 자생적 확산은 어려운 상태이다. STEAM 교육의 확산이 자율적으로 일어나지 않는데는 STEAM 교육의 정체성 부족과 이로 인한 교사들의 혼란, 정책의 변화로 인한 현실과의 괴리가 있기 때문이다. 따라서 교사들의 경험적 지식을 활용한 현장 적용에 관련된 연구에서 벗어나 주로 전문연구자들에 의해 이루어졌던 STEAM 교육에 대한 본질을 교육 실행의 주체인 교사의 눈에서 바라보고 한계와 방향성을 모색할 필요가 있다. 따라서 본 연구에서는 정책 경험과 현장 적용 경험이 모두 풍부한 8인의 초중고 교사로부터 STEAM 교육의 목적, 정의, 준거틀, 변화 등에 대한 설문 및 면담을 실시하였으며, 유형적 분석법을 사용하여 자료를 분석하였다. 분석 결과 STEAM 교육의 목적은 정책적인 목적보다는 교사 개인의 경험에 치중되어 있었으며, 이는 STEAM 교사의 실행 당위성을 감소시키므로, STEAM 교육의 목적을 교사가 직접 경험할 수 있는 융합적 사고력과 문제해결력에 대한 평가 방안이 제시되어야 한다. STEAM 교육의 정의에 사용된 '과학기술 기반' 과 '분야' 대한 단어의 수준 정립은 STEAM 교육의 목표가 명확해지고 꾸준하게 실행될 때 이루어질 수 있다. '과학기술 기반' 이라는 용어를 사용함으로써 교육과정을 넘어 융합 교육을 좀 더 수월하게 할 수 있도록 하였으며, '분야' 또는 '요소' 라는 용어는 정해진 기준 대신 학생들이 하는 활동의 맥락과 수준을 고려하여 상황에 맞게 변할 수 있다. 교수학습 준거틀은 STEAM 교육이 일관성을 유지시키는 가이드라인일 수 있으나 접근을 어렵게 하는 걸림돌이 될 수도 있으므로 좀 더 넓은 의미를 허용할 필요가 있다. 상황제시의 경우 학생의 흥미와 수업의 안내를 강조하고, 실생활 연계의 의미를 학생 수준에서 확대할 필요가 있으며, 창의적 설계는 산출물을 강조하는데서 벗어나 그 과정에 방점을 맞출 수 있도록 해야 하고, 감성적 체험은 현장의 적용 사례를 분석하여 의미와 정의를 수정할 필요가 있다. STEAM 교육이 교육과정에 포함되면서 확대되고는 있으나 여전히 초등에 머물고 있어 중등 교육과정의 편입이 무엇보다 중요하며 시도별 연수의 질 관리, 꾸준한 예산과 제도적 지원이 뒷받침되어야 STEAM 교육에 대한 확신이 높아지고 실행이 활성화될 것이다. 또한 이제까지 지속되는 수많은 STEAM 정책들의 피드백 과정이 보완되어야 수요자 중심의 STEAM 교육이 자리잡을 수 있을 것이다.

한반도 동남부 제4기 단층 비지의 광물학적 및 지구화학적 연구 (Mineralogy and Geochemistry of Quaternary Fault Gouges in the Southeastern Korean Peninsula)

  • 손승완;장태우;김영규
    • 한국광물학회지
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    • 제15권2호
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    • pp.85-94
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    • 2002
  • 한반도 동남부 일대에 발견된 제4기 단층인 상천, 입실, 왕산단층 비 지대에서 단층운동과 이에 수반된 유체의 영향으로 생긴 미구조 및 광물학적인 변화 양상을 알아보기 위해 단층 비지와 주위 모암의 전암분석과 전자현미분석이 수행되었다. 단층 비지와 주위 모암의 X-선형광분석 결과 유동성이 낮은 원소 즉 $TiO_2$, MgO, $P_2$$O_{5}$ , $Fe_2$$O_3$가 신선한 모암에 비해 비지대에 집적되는데 이는 단층운동과 이에 수반된 유체들의 활동이 활발했음을 이야기 해준다. 단층 비지 물질의 X-선회절분석 결과 관찰되는 광물은 석영, 장석, 방해석과 점토광물이고, 점토광물은 대부분 스멕타이트이며 (060) 회절선의 값은 평균 1.50 $\AA$으로써 이팔면체 구조를 가진다. 전자현미분석 결과 비지대 및 모암내 다양한 종류의 황화, 탄산염 및 인산염광물들이 관찰된다. 상천단층 회색 비지대의 제노타임, 입실단층 접촉부 안산암과 왕산단층 접촉부 회색 안산암의 황화광물은 제4기 이전의 단층운동과 관련된 열수의 유입으로 인해 생성된 것으로 추측 된다. 입실 접촉부 안산암과 비지대의 맥상의 탄산염광물은 제4기 이전의 단층운동과 관련된 유체에 의해 생성되었고, 이들 탄산염광물이 물리적으로 심하게 파쇄되어 있으며 탄산염광물 가장자리에 반응연이 있는 것으로 보아 탄산염광물이 생성되고 난 후에도 단층운동과 유체의 활동이 활발했음을 알 수 있다. 왕산단층의 맥상의 방해석은 제4기 단층운동과 동시기 또는 후기에 생성된 것으로 볼 수 있다.>${\mu}c$-Si:H의 Ea 가 가장 낮은 것을 관찰 할 수 있었다.s derived by combining the numerous rainfall-runoff data. The conclusions are as follows; 1)The oscillations in the derived unit hydrograph are reduced by combining the data from each flood event. 2) The reciprocals of the minimum eigen\value of XTX, 1/k and the condition number CN are increased when the oscillations are active in the derived unit hydrograph. 3)The parameter estimates are validated by extending the model to the Soyang river Dam site with elimination of the autocorrelation in the disturbances. Finally, this paper illustrates the application of the multiple regression model to drive an optimal unit hydrograph dealing with the multicollinearity and the autocorrelation which cause some problems. 우선적으로 고려하여 사용할 농약을 선택해야 할 것으로 보이나, 그 외 약제의 잔류성, 사용량, 사용시기와 함께

국경을 넘는 해양환경오염에 대한 국가책임과 적용의 한계 -중국의 산샤댐 건설로 인한 동중국해 해양환경 영향을 중심으로- (Limitations of National Responsibility and its Application on Marine Environmental Pollution beyond Borders -Focused on the Effects of China's Three Gorges Dam on the Marine Environment in the East China Sea-)

  • 양희철
    • Ocean and Polar Research
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    • 제37권4호
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    • pp.341-356
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    • 2015
  • A nation has a sovereign right to develop and use its natural resources according to its policies with regard to development and the relevant environment. A nation also has an obligation not to harm other countries or damage environments of neighboring countries as consequences of such actions of developments or use of natural resources. However, international precedents induce a nation to take additional actions not to cause more damages from the specific acts causing environmental damages beyond national borders, when such acts have economic and social importance. That is to say that there is a tendency to resolve such issues in a way to promote the balance between the mutual interests by allowing such actions to continue. A solution to China's Three Gorges Dam dilemma based on a soft law approach is more credible than relying on a good faith approach of national responsibilities and international legal proceedings since the construction and operation of the dam falls within the category of exercising national sovereign rights. If a large scale construction project such as the Three Gorges Dam or operation of a nuclear power plant causes or may cause environmental damage beyond the border of a nation engaged in such an undertaking, countries affected by this undertaking should jointly monitor the environmental effects in a spirit of cooperation rather than trying to stop the construction and should seek cooperative solutions of mutual understanding to establish measures to prevent further damages. If China's Three Gorges Dam construction and operation cause or contain the possibility of causing serious damages to marine environment, China cannot set aside its national responsibility to meet international obligations if China is aware of or knows about the damage that has occurred or may occur but fail to prevent, minimize, reverse or eliminate additional chances of such damages, or fails to put in place measures in order to prevent the recurrence of such damages. However, Korea must be able to prove a causal relationship between the relevant actions and resulting damages if it is to raise objections to the construction or request certain damage-prevention actions against crucial adverse effects on the marine environment out of respect for China's right to develop resources and acts of use thereof. Therefore, it is essential to cumulate continuous monitoring and evaluations information pertaining to marine environmental changes and impacts or responses of affected waters as well as acquisition of scientific baseline data with observed changes in such baseline. As China has adopted a somewhat nonchalant attitude toward taking adequate actions to protect against marine pollution risks or adverse effects caused by the construction and operation of China's Three Gorges Dam, there is a need to persuade China to adopt a more active stance and become involved in the monitoring and co-investigation of the Yellow Sea in order to protect the marine environment. Moreover, there is a need to build a regular environmental monitoring system that includes the evaluation of environmental effects beyond borders. The Espoo Convention can serve as a mechanism to ease potential conflicts of national interest in the Northeast Asian waters where political and historical sensitivities are acute. Especially, the recent diplomatic policy advanced by Korea and China can be implemented as an important example of gentle cooperation as the policy tool of choice is based on regional cooperation or cooperation between different regions.

주산기 저산소성 허혈성 뇌손상에서 항세포자멸사를 통한 mycophenolic acid의 신경보호 효과 (The neuroprotective effect of mycophenolic acid via anti-apoptosis in perinatal hypoxic-ischemic brain injury)

  • 김지영;양승호;차선화;김지언;장영채;박관규;김진경;정혜리;서억수;김우택
    • Clinical and Experimental Pediatrics
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    • 제50권7호
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    • pp.686-693
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    • 2007
  • 목 적 : Mycophenolate mofetil (MMF)의 활성 대사산물인 (MPA는 IMPDH의 잠재적인 반응 억제제로써 새로운 면역치료제로 사용되고 있다. 이러한 MPA는 신경계에서 흥분독성 손상 후 뇌세포를 보호하고, 미세아교세포에서는 세포사멸사(apoptosis)를 유도하지만, 저산소성 허혈성 뇌질환에서 MPA의 효과는 아직 알려지지 않아, 본 연구에서 Rice-Vannucci 모델을 이용한 신생 백서의 저산소성 허혈성 뇌 손상과 저산소 상태의 태아 백서 뇌세포 배양에서 MPA의 뇌보호 효과를 알아보고자 실험하였다. 방 법 : 생후 7일된 백서의 좌측 총 경동맥을 결찰한 후 저산소 (8% $O_2$) 상태에서 2시간 노출하여, 저산소성 허혈성 뇌 손상을 유발하고 뇌 손상 전후에 MPA(10 mg/kg)를 투여하여 대조군과 비교하였다. 또한, 재태기간 18일된 태아 백서의 대뇌피질 세포를 배양하여 1% $O_2$ 배양기에서 저산소 상태로 세포손상을 유도하여 저산소군, 손상 전후 MPA 투여군($10{\mu}g/mL$)으로 나누어 정상산소군과 비교하였다. 세포사멸사와의 관련을 알아보기 위해서 Bcl-2, Bax, caspase-3 항체로 western blotting하였고 Bcl-2, Bax, caspase-3 primer를 이용하여 real-time PCR을 하였다. 결 과 : 형태학적으로 H&E 염색상 MPA를 투여한 군에서 뇌 보호 효과를 보였다. Western blotting과 real-time PCR을 이용한 저산소성 허혈성 뇌손상 동물 모델뿐만 아니라 저산소 상태로 태아 백서 뇌세포 배양 실험에서도 MPA 투여한 경우 caspase-3의 발현과 Bax/Bcl-2의 비율이 감소함을 보였다. 결 론 : 본 연구에서 MPA가 anti-apoptosis 작용을 통하여 주산기 저산소성 허혈성 뇌 손상에 뇌보호 역할을 하는 것을 알 수 있었고 향후 신생아 저산소성 허혈성 뇌병증의 치료에 임상적 적용이 가능하리라 생각된다.

Onco. Flash Processing 적용에 따른 핵의학 영상의 유용성 평가 (Usefulness in Evaluation of NM Image which It Follows in Onco. Flash Processing Application)

  • 김정수;김병진;김진의;우재룡;김현주;신희원
    • 핵의학기술
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    • 제12권1호
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    • pp.13-18
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    • 2008
  • 목적: 다양한 algorism에 의한 영상처리기법은 핵의학 영상을 결정짓는 중요한 부분을 차지하고 있다. 이에 새로운 영상처리기법인 SIEMENS (made by pixon)사의 Onco. flash processing reconstruction을 적용하여 기존의 영상처리기법을 이용한 영상과 비교 분석함으로써 그 임상적 유용성을 평가한다. 대상 및 방법: 1) Scan speed의 차이에 의한 whole body bone scan을 시행하고, raw data와 processing data의 imaeg quality를 비교 분석하여 상대 평가한다. 2) Bone static scan을 acquisition count를 달리하여 시행하고, raw data와 processing data의 image quality를 비교 분석하여 상대 평가한다. 3) 4 quadrant - bar phantom을 이용하여 raw data와 processing data와의 육안적 평가를 통한 image quality를 확인한다. 4) LSF을 통한 raw data와 processing data의 FWHM을 구하여 해상력 평가를 확인한다. 결과: 1) Whole body bone scan을 시행하여 본원 핵의학 판독의의 blinding test한 결과 scan speed 20 cm/min의 raw data와 30 cm/min의 processing data에는 임상 판독에 영향을 미칠 수준의 image quality 저하가 없었으나, 40 cm/min processing data는 영상 판독과 진단에 오류의 가능성을 배제 할 수 없는 image quality의 향상을 볼 수 없었다. 2) Bone static scan의 경우 200 kcts processing data는 200 kcts raw data보다 확실한 image quality의 향상을 가져왔으며 400 kcts raw data와 비교한 본원 핵의학 판독의 blinding test 결과 판독과 진단에 무리가 없을 수준의 유사한 image quality를 보였다. 3) 4 quadrant - bar phantom을 이용하여 raw data와 processing data와의 육안적 평가는 processing을 통한 image quality의 향상을 확인할 수 있었다. 4) LSF을 통한 raw data와 processing data의 FWHM 평가 결과, resolution의 뚜렷한 증가나 감소의 확인은 할 수 없었다. 이는 noise level의 감소와 high S/N ratio 때문이라 판단된다. 결론: 기존의 영상과 비교 분석하여 평가한 결과 Onco. flash processing reconstruction을 적용한 경우 일정 수준까지 뚜렷한 image quality의 향상을 보였으며, 이는 장비 가동률의 상승과 환자 대기일수의 단축 그리고 저선량 검사에 따른 방사선 피폭에 대한 적극적 방어의 관점에서 현재 임상 핵의학에 충분한 유용성과 타당성이 있을 것으로 사료된다.

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