• Journal of Drive and Control
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• v.20 no.2
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• pp.15-23
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• 2023

In this study, two-dimensional location of crops for auto weeding was detected using deep learning. To construct a dataset for soybean detection, an image-capturing system was developed using a mono camera and single-board computer and the system was mounted on a weeding robot to collect soybean images. A dataset was constructed by extracting RoI (region of interest) from the raw image and each sample was labeled with soybean and the background for classification learning. The deep learning model consisted of four convolutional layers and was trained with a weakly supervised learning method that can provide object localization only using image-level labeling. Localization of the soybean area can be visualized via CAM and the two-dimensional position of the soybean was estimated by clustering the pixels associated with the soybean area and transforming the pixel coordinates to world coordinates. The actual position, which is determined manually as pixel coordinates in the image was evaluated and performances were 6.6(X-axis), 5.1(Y-axis) and 1.2(X-axis), 2.2(Y-axis) for MSE and RMSE about world coordinates, respectively. From the results, we confirmed that the center position of the soybean area derived through deep learning was sufficient for use in automatic weeding systems.

• Physical Therapy Korea
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• v.30 no.2
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• pp.144-151
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• 2023

Background: The gluteus maximus (GM) muscle comprise the lumbo-pelvic complex and is an important stabilizing muscle during leg extension. In patients with low back pain (LBP) with weakness of the GM, spine leads to compensatory muscle activities such as instantaneous increase of the erector spinae (ES) muscle activity. Four-point kneeling arm and leg lift (FKALL) is most common types of lumbopelvic and GM muscles strengthening exercise. We assumed that altered hip position during FKALL may increase thoraco-lumbar stabilizer like GM activity more effectively method. Objects: The purpose of this study was investigated that effects of the three exercise postures on the right-sided GM, internal oblique (IO), external oblique (EO), and multifidus (MF) muscle activities and pelvic kinematic during FKALL. Methods: Twenty eight healthy individuals participated in this study. The exercises were performed three conditions of FKALL (pure FKALL, FKALL with 120° hip flexion of the supporting leg, FKALL with 30° hip abduction of the lifted leg). Participants performed FKALL exercises three times each condition, and motion sensor used to measure pelvic tilt and rotation angle. Results: This study demonstrated that no significant change in pelvic angle during hip movement in the FKALL (p > 0.05). However, the MF and GM muscle activities in FKALL with hip flexion and hip abduction is greater than pure FKALL position (p < 0.001). Conclusion: Our finding suggests that change the posture of the hip joint to facilitate GM muscle activation during trunk stabilization exercises such as the FKALL.

• Journal of the Korean Society of Systems Engineering
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• v.19 no.1
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• pp.64-78
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• 2023

This paper introduces urban air mobility (UAM) and the definition and types of vertiports required for UAM operations. It also examines domestic policy trends related to UAM and identifies UAM routes in Seoul currently planned by the government. To do so, we reviewed prior research on vertiports, analyzed new regulations from the European Aviation Safety Agency, and studied domestic vertiport specifications and deployment plans for UAM operations based on the size of the S-A1 airframe being developed by Hyundai, and applied them to Samsung Station, the core area of the demonstration routes. Next, using the 'Travel Time Savings Ratio', a method for evaluating transportation economics, we compared and analyzed the time taken by passenger vehicles and the time saved by using UAMs to derive a highly economical demonstration route. As a result, the Samsung Station↔Cheongnyangri Station section was found to be the most efficient. These findings are expected to be utilized for adjusting the distribution of UAMs when operating the demonstration route in the future.

• Advances in concrete construction
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• v.14 no.3
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• pp.185-194
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• 2022

For producing cement and concrete, the construction field has been encouraged by the usage of industrial soil waste (or) secondary materials since it decreases the utilization of natural resources. Simultaneously, for ensuring the quality, the analyses of the strength along with durability properties of that sort of cement and concrete are required. The prediction of strength along with other properties of High-Performance Concrete (HPC) by optimization and machine learning algorithms are focused by already available research methods. However, an error and accuracy issue are possessed. Therefore, the Enhanced Deep Neural Network (EDNN) based strength along with durability prediction of HPC was utilized by this research method. Initially, the data is gathered in the proposed work. Then, the data's pre-processing is done by the elimination of missing data along with normalization. Next, from the pre-processed data, the features are extracted. Hence, the data input to the EDNN algorithm which predicts the strength along with durability properties of the specific mixing input designs. Using the Switched Multi-Objective Jellyfish Optimization (SMOJO) algorithm, the weight value is initialized in the EDNN. The Gaussian radial function is utilized as the activation function. The proposed EDNN's performance is examined with the already available algorithms in the experimental analysis. Based on the RMSE, MAE, MAPE, and R² metrics, the performance of the proposed EDNN is compared to the existing DNN, CNN, ANN, and SVM methods. Further, according to the metrices, the proposed EDNN performs better. Moreover, the effectiveness of proposed EDNN is examined based on the accuracy, precision, recall, and F-Measure metrics. With the already-existing algorithms i.e., JO, GWO, PSO, and GA, the fitness for the proposed SMOJO algorithm is also examined. The proposed SMOJO algorithm achieves a higher fitness value than the already available algorithm.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.103-103
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• 2022

Ganghwaljetongyeum (GHJTY) is a complex herbal decoction comprising 18 plants; it is used to treat arthritis. In order to develop a new anti-arthritic herbal medication, we selected 5 out of 18 GHJTY plants by using bioinformatics analysis. The new medication, called ChondroT, comprised water extracts of Osterici Radix, Lonicerae Folium, Angelicae Gigantis Radix, Clematidis Radix, and Phellodendri Cortex. This study was designed to investigate its chondroprotective and anti-inflammatory effects to develop an anti-arthritic herb medicine. ChondroT was validated using a convenient and accurate high-performance liquid chromatography. photodiode array (HPLC-PDA) detection method for simultaneous determination of its seven reference components. The concentrations of the seven marker constituents were in the range of 0.81-5.46 mg/g. The chondroprotective effects were evaluated based on SW1353 chondrocytes and matrix metalloproteinase 1 (MMP1) expression. In addition, the anti-inflammatory effects of ChondroT were studied by Western blotting of pro-inflammatory enzymes and by enzyme-linked immunosorbent assay (ELISA) of inflammatory mediators in lipopolysaccharides (LPS)-induced RAW264.7 cells. ChondroT enhanced the growth of SW1353 chondrocytes and also significantly inhibited IL-1β-induced MMP-1 expression. However, ChondroT did not show any effects on the growth of HeLa and RAW264.7 cells. The expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was induced by LPS in RAW264.7 cells, which was significantly decreased by pre-treatment with ChondroT. In addition, ChondroT reduced the activation of NF-κB and production of inflammatory mediators, such as IL-1β, IL-6, PGE2, and nitric oxide (NO) in LPS-induced RAW264.7 cells. These results show that ChondroT exerted a chondroprotective effect and demonstrated multi-target mechanisms related to inflammation and arthritis. In addition, the suppressive effect was greater than that exhibited by GHJTY, suggesting that ChondroT, a new complex herbal medication, has therapeutic potential for the treatment of arthritis.

• Journal of Korean Physical Therapy Science
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• v.30 no.3
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• pp.1-13
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• 2023

Background: The bridge exercise prevents repeated damage to the tissues around the spine by reducing stimulus transmission to the ligaments and joint capsules, thereby alleviating back pain. It also contributes to strengthening the muscles of the lower extremities. Design: A Single Subject experience design. Methods: This study was conducted on 28 healthy adults in their 20s to 30s and conducted at St. Mary's Hospital in C City from May to July 2021. Four types of bridge exercise were performed in this study: the normal bridge exercise and bridge exercises with 0.5%, 1%, or 1.5% body weight resistance applied on the pelvis through manual resistance during the bridge exercise and to determine the effect of resistance applied in the bridge exercise on the activation of the trunk and lower extremities muscles. Results:This study showed that the muscle activity of the trunk and lower extremities improved significantly in response to stronger resistance when manual resistance equivalent to 0.5%, 1%, or 1.5% of body weight was applied during the bridge exercise compared to when the normal bridge exercise was performed. Conclusion: This study shows that manual resistance can be applied as an effective method of bridge exercise since muscle activity in the trunk and lower extremities increases when manual resistance causing isometric contraction is applied.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.3
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• pp.143-150
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• 2023

Background: The successful production of superior or transgenic offspring from in vitro produced embryos in cattle relies heavily on the quality of blastocyst stage embryos. In order to enhance the developmental competency of these embryos, a novel culture method was devised. Methods: This study utilized stem cell culture medium (SCM) from hESCs as a supplement within the culture medium for bovine in vitro produced embryos. To gauge the efficacy of this approach, in vitro fertilized embryos were subjected to culture in CR1aa medium enriched with one of three supplements: 0.3% BSA, 10% FBS, or 10% SCM. Results: The blastocyst development and hatching rates of one-cell zygotes cultured in CR1aa medium supplemented with SCM (23.9% and 10.2%) surpassed those cultured in CR1aa medium supplemented with BSA (9.3% and 0.0%) or FBS (3.1% and 0.0%) (p < 0.05). Furthermore, post-zygotic gene activation, cleaved embryos cultured in CR1aa medium supplemented with SCM (57.8% and 34.5%) exhibited notably higher rates (p < 0.05) compared to those cultured with BSA (12.9% and 0.0%) or FBS (45.7% and 22.5%) supplementation. Furthermore, the microinjection of SCM into the cytoplasm or pronucleus of fertilized zygotes resulted in elevated blastocyst development and hatching rates, particularly when the microinjected embryos were subsequently cultured in CR1aa medium supplemented with SCM from the 8-cell embryo stage onwards (p < 0.05), in contrast to those cultured with FBS supplementation. Conclusions: In conclusion, this study conclusively demonstrated that the incorporation of SCM into the culture medium significantly enhances the developmental progress of preimplantation embryos.

• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1576-1586
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• 2023

Vaccination is the most effective method for preventing the spread of the influenza virus. Cell-based influenza vaccines have been developed to overcome the disadvantages of egg-based vaccines and their production efficiency has been previously discussed. In this study, we investigated whether treatment with forskolin (FSK), an adenylyl cyclase activator, affected the output of a cell-based influenza vaccine. We found that FSK increased the propagation of three influenza virus subtypes (A/H1N1/California/4/09, A/H3N2/Mississippi/1/85, and B/Shandong/7/97) in Madin-Darby canine kidney (MDCK) cells. Interestingly, FSK suppressed the growth of MDCK cells. This effect could be a result of protein kinase A (PKA)-Src axis activation, which downregulates extracellular signal-regulated kinase (ERK)1/2 activity and delays cell cycle progression from G1 to S. This delay in cell growth might benefit the binding and entry of the influenza virus in the early stages of viral replication. In contrast, FSK dramatically upregulated ERK1/2 activity via the cAMP-PKA-Raf-1 axis at a late stage of viral replication. Thus, increased ERK1/2 activity might contribute to increased viral ribonucleoprotein export and influenza virus propagation. The increase in viral titer induced by FSK could be explained by the action of cAMP in assisting the entry and binding of the influenza virus. Therefore, FSK addition to cell culture systems could help increase the production efficiency of cell-based vaccines against the influenza virus.

• Analytical Science and Technology
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• v.37 no.1
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• pp.47-62
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• 2024

Abu Gurdi area is located in the South-eastern Desert of Egypt which considered as volcanic massive sulfide deposits (VMS). The present work aims at investigating the ore mineralogy of Abu Gurdi region in addition to the effectiveness of the hydrometallurgical route for processing these ores using alkaline leaching for the extraction of Zn, Cu, and Pb in the presence of hydrogen peroxide, has been investigated. The factors affecting the efficiency of the alkaline leaching of the used ore including the reagent composition, reagent concentration, leaching temperature, leaching time, and Solid /Liquid ratio, have been investigated. It was noted that the sulfide mineralization consists mainly of chalcopyrite, sphalerite, pyrite, galena and bornite. Gold is detected as rare, disseminated crystals within the gangue minerals. Under supergene conditions, secondary copper minerals (covellite, malachite, chrysocolla and atacamite) were formed. The maximum dissolution efficiencies of Cu, Zn, and Pb at the optimum leaching conditions i.e., 250 g/L NaCO₃ - NaHCO₃ alkali concentration, for 3 hr., at 250 ℃, and 1/5 Solid/liquid (S/L) ratio, were 99.48 %, 96.70 % and 99.11 %, respectively. An apparent activation energy for Zn, Cu and Pb dissolution were 21.599, 21.779 and 23.761 kJ.mol^-1, respectively, which were between those of a typical diffusion-controlled process and a chemical reaction-controlled process. Hence, the diffusion of the solid product layer contributed more than the chemical reaction to control the rate of the leaching process. High pure Cu(OH)₂, Pb(OH)₂, and ZnCl₂ were obtained from the finally obtained leach liquor at the optimum leaching conditions by precipitation at different pH. Finally, highly pure Au metal was separated from the mineralized massive sulfide via using adsorption method.

• PNF and Movement
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• v.22 no.2
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• pp.233-242
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• 2024

Purpose: The aim of this study was to investigate the effects of different types of unstable loads on core and lower limb muscle activity during squatting. Methods: Nineteen subjects (all females) with resistance experience but no unstable resistance experience participated in the study. Subjects performed squats under three load conditions, and core and lower limb muscle activity was measured during eccentric and concentric contractions. Results: During the eccentric contraction, core and hip flexor activity was significantly higher with the aqua bag than with the barbell or resistance band, and for the quadriceps, the resistance band was significantly higher than the barbell. During the concentric contraction phase, core and hamstring muscle activity was significantly higher with the aqua bag than with the barbell and elastic band (p < 0.05). Conclusion: Squats with an aqua bag increase core and biceps brachii activation and can be recommended as a training method to improve trunk stability.