• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1347-1354
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• 2006

The aims of this study were to establish a simple and effective method for isolating male germline stem cells (GSCs), and to test the possibility of using these cells as a new approach for male infertility treatment. Testes obtained from neonatal and adult mice were manually decapsulated. GSCs were collected from seminiferous tubules by a two-step enzyme digestion method and plated on gelatin-coated dishes. Over 5-7 days of culture, GSCs obtained from neonates and adults gave rise to large multicellular colonies that were subsequently grown for 10 passages. During in vitro proliferation, oct-4 and two immunological markers (Integrin ${\beta}1,\;{\alpha}6$) for GSCs were highly expressed in the cell colonies. During another culture period of 6 weeks to differentiate to later stage germ cells, the expression of oct-4 mRNA decreased in GSCs and Sertoli cells encapsulated with calcium alginate, but the expression of c-kit and testis-specific histone protein 2B(TH2B) mRNA as well as the localization of c-kit protein was increased. Expression of transition protein (TP-l) and localization of peanut agglutinin were not seen until 3 weeks after culturing, and appeared by 6 weeks of culture. The putative spermatids derived from GSCs supported embryonic development up to the blastocyst stage with normal chromosomal ploidy after chemical activation. Thus, GSCs isolated from neonatal and adult mouse testes were able to be maintained and proliferated in our simple culture conditions. These GSCs have the potential to differentiate into haploid germ cells during another long-term culture.

• Journal of the Korean Geosynthetics Society
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• v.19 no.1
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• pp.103-110
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• 2020

The DMM (Deep mixing method) is a construction method in which an improved pile is installed in the soft ground by excavation ground using an auger and then mixing ground stabilizer with soil. Improved pile installed in the soft ground by the DMM may have different compressive strength depending on the properties and characteristics of the soil. In the previous study, laboratory tests were performed on the ground stabilizer for the DMM developed by using the ash of the circulating fluidized bed boiler as a stimulator for alkali activation of the blast furnace slag. And the test results were analyzed to derive the correlation between the unit weight of binder (γ_B) and the uniaxial compressive strength (q_u). In this study, comparative reviews were conducted on the correlations derived from the same laboratory tests on soil material collected from the Saemangeum area and the stability of the site was evaluated by analyzing the test results performed at the site. As a result, the clay collected from the Saemangeum area satisfies the correlation between the unit weight of binder (γ_B) and the uniaxial compressive strength (q_u) derived from the previous study. And the result of the test at the field showed a higher uniaxial compressive strength than the standard strength at the field, indicating excellent stability.

• Korean Journal of Construction Engineering and Management
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• v.17 no.5
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• pp.108-119
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• 2016

Since the introduction of the converted accident rate in Pre-Qualification(PQ) process in 1992, the safety evaluation system has contributed to a reduction of construction accidents and development of the safety management system in public construction projects. As the comprehensive evaluation method the government plans to introduce includes the indicators 'accident rate' and 'death rate per 10,000 workers', the influences on the safety evaluation in the bidding process would be broader in public sector construction projects. However, the current safety evaluation system is operated by different estimating standards in the bidding process. At this point, a study of improvement on the safety evaluation index is required to review its current conditions and to propose its efficient operating method. Therefore, the purpose of this study is to analyze problems of the current safety evaluation system through the questionnaire survey on the qualified workers in construction safety management and to propose an improvement plan for the problems noticed. Our results suggest the standards of the unified process for the safety evaluation index, the size of construction firms needed for accident rate estimation, and the improvement plan for unreported accidents. The proposed improvement plan enables the reasonable estimation and efficient operation of the safety evaluation index, and further, it would contribute to reducing construction accidents through the activation of voluntary safety management by construction firms.

• Journal of the Korea Institute of Building Construction
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• v.18 no.1
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• pp.89-98
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• 2018

Recently, the importance of the construction technology of the free-formed buildings is becoming more significant, as the interest and demand for the free-formed buildings are increasing. However, it takes much time and cost during the construction of free-formed buildings because the current construction technique of freeform concrete segments is manufactured by a formwork. Therefore, in this study, we suggested a new manufacturing process based on the LOM-type 3D printer for freeform concrete segments that can shorten construction time and reduce cost when constructing freeform concrete segments, and we also verified the feasibility of production process through mock-up test. The result shows that the suggested process shortened 47.8% of production time and saved 56.2% of cost compared to the existing steel formwork method. In conclusion, it is expected that the production method of freeform concrete segments using 3D printer will contribute to the improvement of productivity of freeform concrete segments construction and the activation of new construction method for free-formed building construction.

• The Journal of the Korea Contents Association
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• v.17 no.9
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• pp.672-680
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• 2017

Today the distribution of characters in the consumer market based on digital smart phones is expanding, and the characters themselves are sold with independent merchandise, also various application researches about character are being activated. However, depending on the style of worker 's work on the design process of Korea and China regarding characters, there are differences in design characteristics and the diversity of work. In this study, we attempted to investigate the implications of these designers on the development of creative character design through in-depth research and experiment. Therefore, previous researches of cognitive science were investigated and cognitive experiments were conducted on design process for experts. For this research experiment, the initial sketch stage in the character work of Korea and China was recorded by the method of the designer by the subject through the protocol analysis method which is the qualitative research method. We coded the collected language based on this recording experiment and analyzed the problem behavior. We examined how the cognitive acts are done by the designer to develop the characters. The behavior characteristics and the accidental characteristics. The differences of the behavioral characteristics and the accidental characteristics in each step of the character design process were identified. Through these cognitive experiments, we could examine the behavior analysis of the design thinking process in Korea and China. In the field of design practice, we can set the direction of the design work process of the two countries and help us to produce creative and individual results. I think. These studies are expected to contribute to practical application of product marketing and new cooperation development methods in terms of activation of character industry in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.5376-5383
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• 2011

The objective of this study is the development of carbon-recycle technology, that converts carbon dioxide captured from flue gas to carbon monoxide or carbon for reuse in industrial fields. It is difficult to decompose $CO_2$ because $CO_2$ is very stable molecule. And then metal oxide was used as an activation agent or catalyst for the decomposition of $CO_2$ at low temperature. Metal oxides, which converts $CO_2$ to CO or C, were prepared using Ni-ferrite by solid state method and hydrothermal synthesis in this study. TPR/TPO and TGA were used as an analysis method to analyze the decomposition characteristics of $CO_2$. As the results, the reduction area of $H_2$ was high value at 15 wt% of NiO and the decomposition area of $CO_2$ was superior capacity at 5 wt% of NiO. However, TGA data showed contrary results that reduction area of $H_2$ was 28.47wt% and oxidation area by $CO_2$ was 26.95wt% at 2.5 wt% of NiO, one of the Ni-ferrite powders synthesized using solid state method. $CO_2$ decomposition efficiency was 94.66% and it is excellent results in comparison with previous studies.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.5357-5364
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• 2011

The objective of this study is development of carbon recycle technology which convert carbon dioxide captured from flue gas to carbon monoxide or carbon and reuse in industrial fields. Since carbon dioxide is very stable and difficult to decompose, metal oxide was used as activation agent for the decomposition of carbon dioxide at low temperature. Metal oxides which convert $CO_2$ to CO or carbon were prepared using Ba-ferrite by solid and hydrothermal synthesis. TPR/TPO and TGA were used in this study. The results of TPR by H2 and TPO by $CO_2$ showed that Ba-ferrite powders synthesized by hydrothermal method were better than those by solid method. TGA showed contrary results that reduction of Ba-ferrite powders synthesized using solid method by $H_2$ was 21.96 wt%, oxidation by $CO_2$ was 21.24 wt% and 96.72 wt% of $CO_2$ decomposition efficiency showing excellent oxidation-reduction characteristics at $500^{\circ}C$.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.888-895
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• 2020

In the construction industry, off-site construction (OSC) is drawing attention as a production method due to changes in working hours and the supply and demand of manpower. In 1991, there was a policy of spreading and expanding the use of precast concrete (PC) apartment homes, but they have not been actively used so far since they were discontinued due to quality problems. In this study, policy tasks were analyzed to motivate the application of OSC-based PCs in the apartment housing sector, and policy directions were derived by conducting focus group interviews (FGI). Nine policies are suggested regarding the following topics: PC apartment supply quantity provision, priority application of public housing, priority supply of public housing, preferential floor area ratio, funding, tax support, improvement of business area structure, improvement of delivery method, factory certification system, and training of experts. The results of the FGIs are as follows. First, in order to revitalize PC apartment homes, leading efforts from the public sector are required. Second, rather than reorganizing the business sector or introducing a new delivery method, a policy direction that induces the strengthening of cooperation is desirable. Third, PC activation should be promoted on an institutional basis for securing appropriate construction costs and quality.

• Journal of Convergence for Information Technology
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• v.11 no.5
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• pp.30-37
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• 2021

Various devices are connected to the Internet, and attacks using the Internet are occurring. Among such attacks, there are attacks that use malicious URLs to make users access to wrong phishing sites or distribute malicious viruses. Therefore, how to detect such malicious URL attacks is one of the important security issues. Among recent deep learning technologies, neural networks are showing good performance in image recognition, speech recognition, and pattern recognition. This neural network can be applied to research that analyzes and detects patterns of malicious URL characteristics. In this paper, performance analysis according to various parameters was performed on a method of detecting malicious URLs using neural networks. In this paper, malicious URL detection performance was analyzed while changing the activation function, learning rate, and neural network structure. The experimental data was crawled by Alexa top 1 million and Whois to build the data, and the machine learning library used TensorFlow. As a result of the experiment, when the number of layers is 4, the learning rate is 0.005, and the number of nodes in each layer is 100, the accuracy of 97.8% and the f1 score of 92.94% are obtained.

• Clean Technology
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• v.28 no.3
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• pp.210-217
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• 2022

Boron(B) is a rare resource used for various purposes such as glass, semiconductor materials, gunpowder, rocket fuel, etc. However, Korea depends entirely on imports for boron. Considering the global boron reserves and its current production rate, boron will be depleted on earth in 50 years. Thus, a process including proper adsorbent materials recovering boron from seawater is demanded. This research proposed carbonized nanofibers prepared from electrospun PAN(polyacrylonitrile) mats as promising materials to adsorb boron in aqueous solution. First, the mechanism of boron adsorption on carbonized nanofibers was investigated by DFT(density functional method)-based molecular modeling and the calculated energetics demonstrated that the boron chemisorption on the nitrogen-doped graphene surface by a two-step dehydration is possible with viable activation energies. Then, the electrospun PAN mats were stabilized in air and then carbonized in an argon atmosphere before being immersed in the boric acid aqueous solution. Analytically, SEM(scanning electron microscopy) and Raman measurements were employed to confirm whether the electrospinning and carbonization of PAN mats proceeded successfully. Then, XPS(X-ray photoelectron spectroscopy) peak analysis showed whether the intended nitrogen-doped carbon nanofiber surface was formed and boron was properly adsorbed on nanofibers. Those results demonstrated that the carbonized nanofibers prepared from electrospun PAN mats could be feasible adsorbents for boron recovery in seawater.