• Journal of Technology Innovation
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• v.29 no.1
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• pp.1-38
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• 2021

In this paper, an exploratory analysis study was conducted on establishing a strategy to utilize living labs to enhance the innovation of the energy sector. Through the previous research literature, it was possible to confirm the concept, essential components, innovation characteristics of living labs, and types of innovation issues in the energy sector as the theoretical background. Based on this, the case studies of energy living lab (8 overseas, 1 domestic) were analyzed focusing on the possibility of utilizing living lab as an approach to innovation issues in the energy sector, establishing a customized strategy for essential components of living lab and enhancing innovation. It was confirmed that the establishment of a customized strategy for the essential components of the living lab could be a driving force in enhancing innovation, and the Living Lab is effectively used as an approach method for innovation issues(demand management, supply technology, enhance R&D acceptance and promote commercialization, technology policies) in the energy sector. As a result of the case studies, the driving force of each living lab was derived from the viewpoint of contributing to innovation, and strategies for using the living labs for each energy innovation problem were established. This study is an exploratory and descriptive analytical study of the utilization strategy and value of the living lab model as an approach to innovation issues in the energy field, which can provide a living lab strategy framework that has not been tried in the past and enables living lab activation and network formation. It can also be considered to have academic, practical, and policy implications in that it can also contribute.

• Journal of Microbiology and Biotechnology
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• v.27 no.10
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• pp.1855-1866
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• 2017

The synergistic activation mediator (SAM) system can robustly activate endogenous gene expression by a single-guide RNA. This transcriptional modulation has been shown to enhance gene promoter activity and leads to epigenetic changes. Human $interferon-{\gamma}$ is a common natural glycoprotein involved in antiviral effects and inhibition of cancer cell growth. Large quantities of high-purity $interferon-{\gamma}$ are important for medical research and clinical therapy. To investigate the possibility of employing the SAM system to enhance endogenous human $interferon-{\gamma}$ with normal function in innate immunity, we designed 10 single-guide RNAs that target 200 bp upstream of the transcription start sites of the $interferon-{\gamma}$ genome, which could significantly activate the $interferon-{\gamma}$ promoter reporter. We confirmed that the system can effectively and highly activate $interferon-{\gamma}$ expression in several humanized cell lines. Moreover, we found that the $interferon-{\gamma}$ induced by the SAM system could inhibit tumorigenesis. Taken together, our results reveal that the SAM system can modulate epigenetic traits of non-immune cells through activating $interferon-{\gamma}$ expression and triggering JAK-STAT signaling pathways. Thus, this strategy could offer a novel approach to inhibit tumorigenesis without using exogenous $interferon-{\gamma}$.

• The Journal of Korean Physical Therapy
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• v.28 no.2
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• pp.106-111
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• 2016

Purpose: The aim of this study is to determine the effect of glenohumeral (GH) rotation position in modified knee push-up plus exercise (MKPUP) by examining the surface electromyography (EMG) amplitude in serratus anterior (SA), pectoralis major (PM), and upper trapezius (UTz) and the activity ratio of each muscle. Methods: A total of 22 healthy subjects volunteered for the study. Each subject performed the MKPUP at $0^{\circ}$, $45^{\circ}$, and $90^{\circ}$ of GH joint internal rotation. EMG of the SA and PM, UTz was compared between GH rotation positions and each muscle activity ratio. EMG was used to measure the muscle activity in terms of ratios to maximal voluntary isometric contraction (MVIC). Results: The difference in EMG activity during the exercise in three GH joint internal rotation positions was observed with the SA and the PM. The greater the GH joint internal rotation angle was, the lower the activity of the PM. In contrast, the SA showed higher activity. However, the activity of UT was similar under all conditions. The ratio of the SA and the PM was considerably greater at $90^{\circ}$ GH joint internal rotation than at $0^{\circ}$ and $45^{\circ}$. Conclusion: When excessive activation of the PM or imbalanced activation between the PM and the SA occurs, the MKPUP exercise is most effective at $90^{\circ}$ of GH joint internal rotation. Use of this position would be a beneficial strategy for selective strengthening of the SA and minimizing PM activation.

• Physical Therapy Korea
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• v.15 no.2
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• pp.30-37
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• 2008

Anticipatory postural adjustments are pre-planned by the central nervous system (CNS) before the activation of agonist muscles in the limbs, and minimize postural sway. Most previous studies on this topic have focused on upper-limb movement, and little research has been conducted on lower-limb movement. The purpose of this study was to investigate the recruitment order of left and right trunk muscles during limb movement. Fifteen healthy subjects (10 male, 5 female) were enrolled. Electro-myographic signals were recorded on the muscles of: (1) deltoid, lumbar erector spinae, latissimus dorsi and internal oblique during shoulder flexion, (2) rectus femoris, rectus abdominis, external oblique and internal oblique during hip flexion. During right upper limb flexion, the onset of left erector spinae muscle and left internal oblique muscle activity preceded the onset of right deltoid by 8.09 ms and 19.83 ms, respectively. But these differences were not significant (p>.05). A similar sequence of activation occurred with lower limb flexion. The onset of left internal oblique muscle activity preceded the onset of right rectus femoris muscle by 28.29 ms (p<.05). The onset of right internal oblique muscle activity preceded the onset of left rectus femoris muscles by 23.24 ms (p<.05). The internal oblique muscle was the first activated during limb movement. Our study established the recruitment order of trunk muscles during limb movement, and explained the postural control strategy of the trunk muscles in healthy people. We expect that this study will be used to evaluate patients with an asymmetric recruitment order of muscle activation due to impaired CNS.

• Archives of Plastic Surgery
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• v.40 no.6
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• pp.676-686
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• 2013

Background To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-${\beta}$1 (LTGF) into an electrospun poly(L-lactide) scaffold. Methods The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats. Results Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen. Conclusions We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering.

• Environmental Engineering Research
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• v.20 no.2
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• pp.141-148
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• 2015

The presence of phenols in treated palm oil mill effluent (POME) is an environmental concern due to their phytotoxicity and antimicrobial activity. In this study, phenol-degrading bacteria, Methylobacterium sp. NP3 and Acinetobacter sp. PK1 were immobilized on oil palm empty fruit bunches (EFBs) for removal of phenols in the treated POME. The bacterial exopolysaccharides (EPS) were responsible for cell adhesion to the EFBs during the immobilization process. These immobilized bacteria could effectively remove up to 5,000 mg/L phenol in a carbon free mineral medium (CFMM) with a greater degradation efficiency and rate than that with suspended bacteria. To increase the efficiency of the immobilized bacteria, three approaches, namely activation, acclimation, and combined activation and acclimation were applied. The most convenient and efficient strategy was found when the immobilized bacteria were activated in a CFMM containing phenol for 24 h before biotreatment of the treated POME. These activated immobilized bacteria were able to remove about 63.4% of 33 mg/L phenols in the treated POME, while non-activated and/or acclimated immobilized bacteria could degrade only 35.0%. The activated immobilized bacteria could be effectively reused for at least ten application cycles and stored for 4 weeks at $4^{\circ}C$ with the similar activities. In addition, the utilization of the abundant EFBs gives value-added to the palm oil mill wastes and is environmentally friendly thus making it is attractive for practical application.

• Journal of Korean Society of Rural Planning
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• v.19 no.1
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• pp.91-107
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• 2013

In recent years, the number of walking-tourists who visit Jeju olle trail are increasing every year and the ripple effect is a rapid change on the roles and leadership of local people in rural villages. The reason for the change is that most Jeju Olle Trails cuts through the rural village in Jeju island. However, most of travelers just walked along the trails, no one was interested about villages along the Olle roads. For these reasons, many rural villages would not get any benefits such as non-farm income, sales profit and also can not activate the facility of village and infrastructure. Therefore, we study on Revitalization Method for rural villages through the Analysis of characteristics of tourist in Jeju Olle Trails and a field survey research too. In this process, we investigated the status and characteristics of the rural villages of Jeju island in the first step. Then, we considered the distinct characteristics and factors for improving of the 10 places with site investigation where were recommended by Jeju Olle Corporation and Jeju Agricultural Technology Center in second step. Lastly, we arranged the resident's requirement and result of survey and interview with leaders of rural village. As a result, we suggest the activation methods for residents and leaders of rural area and this study is also expected to help management of rural villages and also is an important study to improve importance of utilizing of more people visiting in Jeju Olle Trails.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.1
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• pp.133-140
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• 2018

The purpose of this paper is to provide baseline on the current status and the activation of BIM in civil engineering which can integrate and manage the information and tasks generated during the life cycle. Hierarchical analysis was conducted to identify the level of BIM application, recognition survey for practitioners, and activation plans. The survey results show similar trends in BIM activation inhibition factor, the need for BIM, the effect of BIM and the future possibility of BIM. The utilization of BIM and the technical training in the civil engineering field were lower than those in the architectural field. The analysis of AHP(Analytic Hierarchy Process) is performed to identify the degree of importance in financial, operational, investment, technical, and institutional sections as its activating factors. As a result, the technical factor points the highest degree. Thus, study shows the direction to apply BIM in civil engineering in the future by suggesting an efficient plan.

• BMB Reports
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• v.52 no.12
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• pp.706-711
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• 2019

Cisplatin (Cis-DDP) is one of the most widely used anti-cancer drugs. It is applicable to many types of cancer, including lung, bladder, and breast cancer. However, its use is now limited because of drug resistance. p90 ribosomal S6 kinase (p90RSK) is one of the downstream effectors in the extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) pathway and high expression of p90RSK is observed in human breast cancer tissues. Therefore, we investigated the role of p90RSK in the Cis-DDP resistance-related signaling pathway and epithelial-mesenchymal transition (EMT) in breast cancer cells. First, we discovered that MDA-MB-231 cells exhibited more Cis-DDP resistance than other breast cancer cells, including MCF-7 and BT549 cells. Cis-DDP increased p90RSK activation, whereas the inactivation of p90RSK using a small interfering RNA (siRNA) or dominant-negative kinase mutant plasmid overexpression significantly reduced Cis-DDP-induced cell proliferation and migration via the inhibition of matrix metallopeptidase (MMP)2 and MMP9 in MDA-MB-231 cells. In addition, p90RSK activation was involved in EMT via the upregulation of mRNA expression, including that of Snail, Twist, ZEB1, N-cadherin, and vimentin. We also investigated NF-κB, the upstream regulator of EMT markers, and discovered that Cis-DDP treatment led to NF-κB translocation in the nucleus as well as its promoter activity. Our results suggest that targeting p90RSK would be a good strategy to increase Cis-DDP sensitivity in triple-negative breast cancers.

• Journal of Digital Convergence
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• v.9 no.3
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• pp.59-67
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• 2011

The World aerospace industry grows consistently with the development digital technology. We study the activation of the digital aerospace industry based on avionics system, with respect to demand, system and industrial structure in the gimpo area. The strategy is made for aerospace industry to be a growth driver and then we suggest some projects to carry out it. Those are lasting creation of aerospace demand, possession of core part material and competitive digital technology, construction infrastructure for activation of system operation, improvement of administrative assistance system and finally strengthening policy modulation between civil, company, army, academy, and government. Therefore, it is possible to develop into it focusing the MRO and the Helicopter, if we complement systems related in the field of administration and Industry.