• Archives of Pharmacal Research
• /
• v.21 no.1
• /
• pp.6-11
• /
• 1998

The activities of two types of antiulcer agents against 9 strains of Helicobacter pylori (H. pylori) were determined by the agar dilution method. The antiulcer agents were YJA20379, a newly synthesized proton pump inhibitor developed by Yung-jin Pharmaceutical company, and omeprazole. Both compounds were found to have significant activities against this organism. The MIC values of YJA20379 and omeprazole were 11.7 and $31.25{\mu.g/ml}$ respectively. In addition, the inhibitory potency of both compounds was investigated on H. pylori urease which is believed to be an important colonization and virulence factor in the pathogenesis of gastritis and peptic ulcers. These compounds dose-dependently inhibited urease extracted with distilled water and their $IC_50$ values were $16.4{\times}10^{-5} M and 14.3{\times}10^{-5}M,$ respectively. In addition, a pH-dependent study to determine whether inhibitory potency would be activated by acid condition was performed. It was found that unlike omeprazole, YJA20379 was not affected by acid condition. To determine the inhibition pattern and optimal concentration of substrate, kinetics were evaluated at various pH levels (pH 5.0, 7.0, and 8.5). The data show that YJA20379 noncompetitively inhibited H. pylori urease and $K_M/K_i$values were 0.96 $mM/60{\mu}M (pH 5.0), 0.56 mM/141.5 {\mu}M (pH 7.0)$, and $1.94mM/34{\mu}M (pH 8.5)$, respectively. Based on data obtained, it is concluded that YJA20379 is a significant inhibitor of H. pylori growth and urease and therefore, taking these results into consideration, YJA20379 might be a beneficial therapy for gastritis and peptic ulcers induced by H. pylori.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• v.14 no.1
• /
• pp.3-11
• /
• 2003

Attention-deficit/Hyperactivity disorder(ADHD) is the most common psychiatric disorder of childhood and among the most prevalent chronic health conditions affecting school-aged children. Children with ADHD experience significant functional problems, such as school difficulties, academic underachievement, troublesome interpersonal relationships with family members and peers, and low self-esteem. The most widely used pharmacological treatments for ADHD are psychostimulants, such as methylphenidate and amphetamine salts. These medications provide clinical efficacy by increasing the availability of catecholamines, primarily dopamine, in the frontal lobe of the brain. immediate-release(IR) formulations of sychostimulants were among the most effective psychotrophic medications in the psychopharmacological treatment. However, there are some limitations of IR formulations：the short half-life and duration of efficacy, which result in the need for multiple daily dosing and the poor compliance. These limitations have led to the development of once-daily, extended-release(ER) formulations of methylphenidate and amphetamine salts. However, these ER formulations may not be as immediately helpful to ADHD children due to delayed onset of action and the acute tolerance which is the failure to sustain the efficacy with the same concentration of drug as the initial stage of medication. OROS-methylphenidate(Concerta$^{\circledR}$) given once a day produces an ascending-pattern plasma drug level generated by the osmotically released, timed drug-delivery system. These new formulations of the psychostimulants have been shown to be a useful alternative to old stimulant medications through the evidence by the clinical trials.

• Animal Systematics, Evolution and Diversity
• /
• v.18 no.1
• /
• pp.13-21
• /
• 2002

To add genetic information to the conservation efforts on grey coral (Naemorhedus caudatus) in Korea, we investigated the pattern of mitochondrial cytochrome b gene sequence (606 bp) of six specimens from two localities in Korea. The corresponding sequences of N. caudatus in China obtained from GenBank were also used. The nucleotide Tamura-Nei distances between each of four haplotypes of N. caudatus in Korea and the haplotype of N. caudatus in China varied from 0.0650 to 0.0803: N. caudatus revealed high level of sequence diversity in Bovidae. In N. caudatus in Korea, the distances among three haplotypes at Yanggu were 0.0151 to 0.0185, and it suggests that the genetic diversity of Yanggu population was decreased in low level. Moreover, the distances between each of three haplotypes at Yanggu and one haplotype at Samcheok were 0.0343 to 0.0489. It indicates that habitat isolation caused the continuous increase of genetic distance with geographic distance in N. caudatus, and various conservation plans for mitigating the loss of genetic diversity in Korea have to be in immediate action. To clarify the taxonomic status of N. caudatus, the sequence (276 bp) of N. goral available from GenBank were also utilized, and n goral was not distinct from N. caudatus. It suggests that they may be conspecific, but further analyses with additional specimens of two species are necessary.

• Korean Journal of Agricultural Science
• /
• v.47 no.3
• /
• pp.615-624
• /
• 2020

An effective sampling method is necessary to monitor potato tuber moths (Phthorimaea operculella) because they are the biggest concern in potato-cultivating areas. In this study, a sequential sampling method was developed based on the results of field surveys of potato tuber moths in South Korea. Potato tuber moths were collected in fields cultivating potatoes at six sites, and their spatial distribution was investigated using the Taylor power law. The optimal sampling size and cumulative number of potato tuber moths in traps to stop sampling were determined based on the spatial distribution pattern and mean density of the collected potato tuber moths. Finally, the developed sampling method was applied to propose a control action, and its sampling efficiency was compared with that of the traditional sampling method using a binomial distribution. The potato tuber moths tended to aggregate; the optimal number was approximately 5 - 16 traps for sampling, and the number varied with the mean density of potato tuber moths according to the sampling sites. In addition, one, two, and three sites might require the following actions: Continued sampling, control, and no control, respectively. Sampling with the binomial distribution showed the minimum sample size was 12 when considering the economic threshold level. Here, we propose an effective sampling method that can be applied for future monitoring and field surveys of potato tuber moths in South Korea.

• Journal of The Korean Association For Science Education
• /
• v.30 no.6
• /
• pp.799-814
• /
• 2010

This study aimed at developing a set of question cards for fostering deep understanding and encouraging reasoning about fossils and analyze the characteristics of visitors' communication depending on whether to use the question cards in a fossil gallery. Through several steps, a card set consisted of nine generic questions about fossil exhibitions and guidance for using question cards were developed. Data related to visitors' communications were collected from 18 peer groups (from 5th to 9th grade) visiting the fossil gallery of Gwacheon National Science Museum. Visiting groups' interactions were videotape recorded and transcribed. 'Holding time,' the types of 'actions,' and the types of 'conversation' were analysed. Visitors' actions were divided into three categories: ‘look’, 'speech', and 'motion.' Furthermore, visitors' conversations categorized as 'speech' were subdivided into four patterns: 'enumerative,' 'consensual,' 'responsive,' and 'argumentative.' Using the question cards contributes to increase holding time and most of the visiting actions. Most of the conversation patterns also increased except the responsive pattern. In conclusion, using question cards in a fossil gallery could facilitate concentrated and meaningful visits by enhancing active verbal and non-verbal communications between exhibit and visitor or among visitors, encouraging visitors' reasoning about exhibits, and guiding visitors what and how to focus on exhibits.

• Molecules and Cells
• /
• v.37 no.11
• /
• pp.833-840
• /
• 2014

Cullin4-RING ubiquitin ligase (CRL4) is a family of multi-subunit E3 ligases. To investigate the possible involvement of CRL4 in heat stress response, we screened T-DNA insertion mutants of putative CRL4 substrate receptors that exhibited altered patterns in response to heat stress. One of the mutants exhibited heat stress tolerance and was named heat stress tolerant DWD1 (htd1). Introduction of HTD1 gene into htd1-1 led to recovery of heat sensitivity to the wild type level, confirming that the decrease of HTD1 transcripts resulted in heat tolerance. Therefore, HTD1 plays a negative role in thermotolerance in Arabidopsis. Additionally, HTD1 directly interacted with DDB1a in yeast two-hybrid assays and associated with DDB1b in vivo, supporting that it could be a part of a CRL4 complex. Various heat-inducible genes such as HSP14.7, HSP21, At2g03020 and WRKY28 were hyper-induced in htd1-1, indicating that HTD1 could function as a negative regulator for the expression of such genes and that these genes might contribute to thermotolerance of htd1-1, at least in part. HTD1 was associated with HSP90-1, a crucial regulator of thermotolerance, in vivo, even though the decrease of HTD1 did not affect the accumulation pattern of HSP90-1 in Arabidopsis. These findings indicate that a negative role of HTD1 in thermotolerance might be achieved through its association with HSP90-1, possibly by disturbing the action of HSP90-1, not by the degradation of HSP90-1. This study will serve as an important step toward understanding of the functional connection between CRL4-mediated processes and plant heat stress signaling.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.21 no.2
• /
• pp.195-200
• /
• 1992

Rhizopus oryzae CJ-2114 was selected for its strong polygalacturonase activity among various strains of mold found in soil. The optimum pH for the enzyme activity was 4.0 and optimum temperature was 4$0^{\circ}C$. The activation energy for the polygalacturonase was calculated by Arrhenius equation was 2.048㎉/㏖. The reaction of this enzyme followed typical Michaelis-Menten kinetics with the Km value of 54.05mM with the $V_{max}$ of 13.9m mole/min. The enzyme is relatively stable in acidic condition. The activity of polygalactur-onase was inhibited completely by C $u^{2+}$, P $b^{2+}$ and Z $n^{2+}$, $_Mn^{2+}$ at concentration of 1 mM. The enzyme can be inactivated by the treatment with maleic anhydride and iodine. The results indicate the possible involvement of histidine at active site. When polygalacturonase from Rhizopus oryzae CJ-2114 was reacted with poly-galacturonic acid as a substrate mono-, di-, and oligogalacturonic acid were produced at early and mono-, digalacturonic acid produced at late incubation time. time.

• Journal of Environmental Science International
• /
• v.2 no.2
• /
• pp.153-160
• /
• 1993

The problem of supply and transport of sediment from a mountainous catchment is very important in explaining dynamic geomorphology and the hydrological cycle. The discharge of suspended sediment is determined by a morphological system. Human interference to environment Is also an important, not negligible factor in sediment production. Moreover, growing concern in recent years for the problems of nonpoint pollution and for the transport of contaminants through terrestrial and aquatic ecosystems has highlighted the role of sediment-associated transport in fluvial systems. This study was conducted in forested and quarried catchments in order to clarify the different discharge process and the mechanism of suspended sediment dynamics for each catchment. As a forested catchment, the Yamaguchi River catchment which drains a $3.12km^2$ area was chosen. On the other hand, the Futagami River basin which is formed by three subbasins (1.07, 1.59 and $1.78km^2$), as a quarried catchment was selected. These catchments are situated to the north and east of Mt. Tsukuba, Ibaraki, Japan. The discharge pattern of suspended sediment from the Futagami River basin is more unstable and irregular than that from forested catchment, the Yamaguchi River catchment. Under the similar rainstorm conditions, suspended sediment concentration from quarried catchment during a rainstorm event increases from 43 to 27,340 mg/l. However, in the case of the forested catchment it changes only from nearly zero to 274 mg/l. Generally, the supply source of suspended sediment is classified into two areas, the in-channel and non-channel source areas. As a result of field measurements, in the case of the forested catchment the in-channel (channel bed, channel bank and channel margin) is the main source area of suspended sediment. On the other hand, remarkable sediment source area on the Quarried catchment is the non-channel that is unvegetated ground.

• Applied Biological Chemistry
• /
• v.41 no.7
• /
• pp.489-495
• /
• 1998

The optimum culture condition of Scopulariopsis brevicaulis for the production of ${\alpha}$-galactosidase was as follows: Tryptone 1.5%, $NH_4NO_3$ 0.2%, Raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$. The optimum pH and temperature for the enzyme activity of ${\alpha}$-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and $27^{\circ}C$, respectively. The enzyme was relatively stable at $pH\;6.0{\sim}8.0$ and at temperature below $40^{\circ}C$. The activity of the enzyme was inhibited by $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$, p-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for $p-nitrophenyl-{\alpha}-D-galactopyranoside$ and Vmax value was $9.66{\times}10^2\;{\mu}M/min$. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.26 no.1
• /
• pp.93-100
• /
• 2008

The parameters of lens such as focal length, focus, and aperture stop changes while shooting the scenes with zoom lens. Especially, zooming action dramatically changes the geometry of lens system that causes significant change of lens model. We investigated how the lens model changes while zooming in general shooting condition. Each parameters of lens model was estimated and checked whether they can be modeled well in the condition of auto-controlling focus, aperture and vibration reduction. In order to do this, calibration images were taken, modeled in different fecal length setting. And changing patterns of models were inspected to find out if there is some elements that have some particular pattern in changing with respect to focal length. The result showed us that although we didn't control the focus and aperture setting, there's specific changing patterns in radial and do-centering distortion. Especially, the strong linear correlation was found between coefficient of $r^2$ and focal length. It is expected that many parts of distortion can be eliminated without additional self calibration even if zoom operation is done when shooting the scenes if we know its fecal length and model of this coefficient.