• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.68-78
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• 1997

Laboratory and greenhouse studies were conducted to determine differential sensitivities on absorption of $^{14}$ C-oxyfluorfen and the anatomical responses in wheat and barley to protoporphyrinogen oxidase-inhibiting herbicides [oxyfluorfen (2-chloro-1- (3-ethoxy -nitrophen-oxy)-4-(trifluoromethyl) benzene, acifluorfen(5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitro-benzoic acid), bifenox(methyl-5-(2, 4-dichlorophenoxy)-2-nitrobenzoate) and oxadiazon(5-tert-butyl-3-(2, 4-dichloro-5-isopropoxyphenyl)-1, 3, 4-oxadiazol-2-one)]. I$_{50}$ value of the tolerant wheat cultivars to oxyfluorfen was about 10$^{-4}$ , whereas that of the susceptible barley cultivars was about 10$^{-6}$ M, showing significant difference between the two groups. When foliage were applied with acifluorfen, bifenox or oxadiazon, the oxyfluorfen-tolerant wheat showed less decreased in shoot fresh weight and chlorophyll content than the susceptible barley. Also, when soil-applied with these herbicides test plants showed similar tendency in foliar application. Electrolyte leakage from the tissue treated with these compounds was the more influenced in the barley than the wheat. Malondialdehyde(MDA) production as index of lipid peroxidation was greater in the barley than the wheat by treatment of these compounds. Therefore, the differential sensitivities of wheat and barley to protoporphyrinogen oxidaseinhibiting herbicides was showed by our greenhouse and in vitro experiment. The absorption rates of $^{14}$ C-oxyfluorfen were higher in the barley than the wheat. And this tendency was showed appararitly difference by increase of treatment durations. After the oxfluorfen and oxadiazon treatment, the tolerant wheat did not show the structural damage in leaf surface, but the susceptible barley was damaged in the leaf waxy layer. However, the acifluorfen and bifenox treatment showed no difference between wheat and barley. The anatomical changes by these compounds treatment were not observed in the tolerant wheat but epidermal cell and mesophyll cell were highly broken in the susceptible barley.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.52-60
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• 2005

The objectives of present study were to investigate the hepatoprotective and antioxidative effects of onion extracts. Primary cultures of rat hepatocytes were incubated with 1.5 mM tert-butyl hydroperoxide(t-BHP), potent oxidizing agent for liver injury for 1 hr in the presence or absence of various concentrations (0, 0.01, 0.05, 0.1 or 0.3 mg/ml) of onion extract. Cytotoxicity and cell viability were determined by measuring glutamic oxaloacetic transaminase(GOT) activity, lactate dehydrogenase(LDH) activity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) value. Lipid peroxidation was evaluated using thiobarbituric acid reactive substances(TBARS) assay. Effects on antioxidant system were determined by measuring catalase, glutathione peroxidase(GSH-Px), glutathione reductase(GSH-Rd) activities as well as DNA strand breaking assay. Incubation with t-BHP alone increased GOT and LDH activities and TBARS concentration but decreased MTT reduction. Onion extracts at the concentration of 0.05 mg/ml began to decrease GOT and LDH activities induced by 1.5 mM t-BHP. Decreased MTT reduction began to be increased by onion extract at the concentration of 0.01 mg/ml. Onion extracts at the concentration of 0.01 mg/ml began to decrease TBARS concentration induced by t-BHP. Taken together, onion extracts prevented t-BHP-induced hepatocyte injury and lipid peroxidation. Catalase, GSH-Px and GSH-Rd activities of hepatocytes were significantly decreased by 1.5 mM t-BHP for 1 hr incubation. Onion extracts, on the other hand, at the concentration of 0.1 mg/ml began to prevent t-BHP-induced decrease in catalase, GSH-Px and GSH-Rd activities. Onion extracts prevented hydroxyl radical-induced single-strand breakage in dose-dependent manner when plasmid DNA was incubated with various concentrations of onion extracts in the presence of Fenton regents producing hydroxyl radical. These results demonstrate that onion extracts suppressed t-BHP-induced cytoctoxicity, decreased viability and lipid peroxidation and increased GSH-Px, GSH-Rd and catalase activities. Thus hepatoprotective and antioxidant effects of onion extract seem to be due to, at least in part, the increase in antioxidant enzyme activities as well as prevention from hydroxyl radical-induced oxidation, followed by inhibition in lipid peroxidation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.2
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• pp.121-128
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• 2003

This study was conducted to investigate the effect of tall type mixtures and tall+short type mixtures on dry matter yield, botanical composition and quality. The experimental design includes two mixture types: Tall type mixtures(TM); orchardgrass(Potomac) 40+tall fescue(Fawn) 20+Festulolium braunii (Paulita) 10+perennial ryegrass(Reveille) 10+timothy(Climax) 10+red clover(Kenland) 5+alfalfa(Vernal) 5%) and tall+short type mixtures(TSM); orchardgrass(Potomac) 40+tall fescue(Fawn) 20+Kentucky bluegrass(Newport, turf type) 10+redtop(Barricuda, turf type) 10+perennial ryegrass(Palmer III, turf type) 10+red fescue(Flyer II, turf type) 5+white clover(Regal) 5%. The dry matter(DM) yield was higher obtained in TSM than that of TM, but there was no significant difference between TM and TSM. In the chemical composition, there was no significant difference between TM and TSM, but the content of cellulose, lignin were higher in TM than those in TSM(p<0.05). Also, the content of acid detergent fiber which affected dry matter digestibility was higher in TM than that of TSM. The crude protein dry matter yield was not observed significant difference between TM and TSM, but digestible dry matter(DDM) yield was higher in TSM than that of TM. In botanical composition, tall fescue, alfalfa and orchardgrass were highly maintained in TM but white clover, tall fescue and orchardgrass were highly, and redtop, red fescue were continuously maintained in TSM. In this experiment, DM yields and quality of two mixture types were no difference. However, the tall+short type mixtures(TSM) were more effective in enhancing the dry matter digestibility and DDM yield, and in maintaining the various botanical composition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.239-248
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• 2006

This study was conducted not only to investigate effects of different supplementation levels of Acanthopanax senticosus leaves on feed intakes, nutrient digestibility, nitrogen retention, and blood metabolites in Korean black goats but also to obtain basic data for nutritive value of Acanthopanax senticosus leaves and production of high quality functional animal products. Twelve Korean black goats were allotted to treatments in four groups of three goats per treatment and then they were housed in individual metabolism cages with completely random arrangements for 21 days. Four treatments were separated into controls and three different supplementation levels of Acanthopanax senticosus such as 10, 20 and 30%, respectively. For the chemical composition of experimental diets, crude protein (CP) contents of controls were 13.39% and those of supplementation treatments were lowered with increasing levels of Acanthopanax senticosus. Control groups tended to have higher acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents as compared to supplementation groups, but non-fibrous carbohydrate (NFC) contents tended to be higher far supplementation groups than those for controls. Feed intakes had no significant difference among treatments, but digestible dry matter amounts were significantly higher for 20 and 30% supplementation groups than those for other treatments. Dry matter intakes per metabolic body weight and their ratio per body weight were highest in 20% group, but those of 40% groups were lowest (p<0.05). Crude protein, ADF and NDF digestibilities were significantly higher in Acanthopanax senticosus supplementation groups in comparison to controls (p<0.05), and in particular, they were increased with increasing supplementation levels of tranthopanax senticosus. Nitrogen retention was significantly higher for 20 and 30% treatments than those fur controls (p<0.05). Plasma urea nitrogen concentration was significantly lower for 30% supplementation group than those fur controls (p<0.05). Furthermore, plasma triglyceride concentration was significantly lower for Acanthopanax senticosus groups than those for controls (p<0.05). The results showed that supplemental level of 20% Acanthopnnax senticosus leaves to Korean black goats improved nutrient digestibility, nitrogen retention, and plasma urea nitrogen and triglyceride concentrations. Consequently, it might contribute to more efficient production of Korean black goats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.5
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• pp.744-751
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• 1995

Effect of licorice root(Glycyrrhiza Uralensis Fischer) on the Dongchimi(watery radish kimchi) fermentation was investigated by measuring physicochemical, microbiological and sensory properties during fermentation up to 41 days. Dongchimi with the various levels(0, 0.5, 1.0, 1.5%) of licorice root was fermented at two temperatures : Group A was fermented at $10^{\circ}C$ and group B was fermented at $4^{\circ}C$ after keeping at room temperature($16{\pm}0.5^{\circ}C$) for 24 hours. During the fermentatiion, pH was slowly lowered in all Dongchimi samples and pH of Dongchimi added licorice root was a little higher than that of Dongchimi without licorice root. Total acidity of group A was slightly larger than that of group B and Dongchimi added licorice root showed small content than that of Dongchimi without licorice root. Reducing sugar content was reached maximum value in 13 days of fermentation in Dongchimi added licorice root of group A and B, group A decreased remarkably in 32 days of fermentation in all samples and group B was maintained in the rest sample except for Dongchimi without licorice root and Dongchimi added 1.5% licorice root. Total vitamin C of Dongchimi added licorice root was slightly higher than that of Dongchimi without licorice root of two groups. Lightness lowered gradually with the fermentation and Dongchimi without licorice root decreased a little than that of Dongchimi added licorice root. The number of lactic acid bacteria in Dongchimi added licorice root was more numerous than that of Dongchimi without licorice root. As a result of the sensory evaluation, group B showed higher scores than group A and Dongchimi added 0.5% licorice root was the most preferable one.

• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.415-423
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• 1995

This study was conducted to analyze the chemical composition, nutritional contents, and saponin in the xylem sap of Acer mono Max. From Feb 25 to Mar 4, 1994, spring sap was collected by making holes with 1.7cm diameter on the trunk of trees in Mt. Baekwoon and Mt. Jiri. Sugars were quantified by HPIC, amino acids by amino acid analyzer, saponins by TLC, HPLC, and $^1H$ & $^{13}C$ NMR. Major component of the sap was sucrose at a concentration range of 0.68 to 2.01%. Following minor components were found: glucose at 0.03-0.11%, and fructose at 0.01-0.03% as sugars, lipid at 0.03%, threonine at 0.152%, lysine at 0.038%, arginine at 0.068% as amino acids, ash at 0.1%, Ca at 175ppm, Fe at 2ppm, P at 19ppm, K at 16ppm, Na at 31ppm as minerals, vitamin $B_1$ at 0.6ppm, vit. $B_2$ at 0.1ppm, and vit. C at 19ppm. A trace of phenolic compounds was found by TLC, while saponin commonly reported in high-quality ginseng roots was not found in maple sap. It is concluded that sap of Acer mono contains a good variety of natural compounds such as sugars, amino acids, Ca, Fe, and vitamins to serve as an excellent source of very natural and health-promoting drink.

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.157-168
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• 2020

The sensitivity of forest soil to acidification in the Republic of Korea (ROK) was evaluated based on pH_H2O, cation exchange capacity (CEC), and base saturation (BS). Sensitivity to acidification was categorized into three grades: adequate level (AL, pH ≧ 4.2, CEC ≧ 15cmol/kg, BS ≧ 15%), caution level (CL, at least one indicator is below AL), and severe Level (SL, all three indicators are below AL). Soil samples were collected from the 65 stationary monitoring plots (40 × 40 ㎢), distributed throughout ROK. Only 19% of soil samples were classified as AL, while 66% and 15% were CL and SL, respectively. The median of pH_H2O, CEC, BS, and Ca/Al indicator in AL soils was pH 4.64, 20.7cmol/kg, 29%, and 6.3, respectively. Moreover, BC_ex (K⁺, Mg²⁺, Ca²⁺) and available phosphorus (AP) concentration compared with a threshold value and molar ratio of BC_ex and AP to total nitrogen (TN) was high. This indicates that AL soils have a good nutrient condition. The molar Ca/Al ratio, an indicator for toxicity of exchangeable aluminum (Al_ex), was more than 1, indicating no negative impact of Al_ex on plant growth. On the contrary, the median of pH_H2O, CEC, and BS in SL soils was pH 4.02, 13.2cmol/kg, and 10%, respectively. The Ca/Al index was less than 0.6, which indicates that negative impacts of Al_ex on plants were high. Furthermore, both the concentration of BC_ex in SL soils and the BC_ex/TN ratio were the lowest among the three acidity degrees. This shows that SLsoils can be degraded by soil acidification compared with less acidic soils.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.7
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• pp.869-878
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• 2008

The purpose of this study was to obtain baseline data on nutritional management of women's bone health. We examined the bone mineral density (BMD) by ultrasound bone densitometer, anthropometric measurement and dietary intake to assess the nutrient intakes. The subjects were 102 Korean female college students (normal=59, bone disease group [osteopenia+osteoporosis]=43) and the mean age was 21.4 yr. Mean T-score (BMD) were -0.42 and -1.52 in normal and bone disease groups, respectively. Anthropometric measurement showed that 59% of the subjects were normal weight and bone disease group had lower value than normal group in majority of anthropometric index. The average energy intake was 1539.7 kcal, which was 73% of Korean EER. The subjects had lower vitamin C, folic acid, Ca and K intake than Korean RI independent of BMD. Bone disease group (1:2.05) showed significantly lower Ca:P ratio than normal group (1:1.86). Normal group had more intake frequency of milk and milk product than bone disease group. In relation to anthropometric index with T-score, significant correlations have been found in weight, PM, BFM, MM, TBW, SLM, FFM WHR, BMI and SMM. In relation to dietary factors with T-score, significant correlations have been found relating to intake frequency of milk and milk product. Our results indicate that for nutrients, ratio and balance may be more influential than intake for bone health in young women.

• Journal of Life Science
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• v.26 no.11
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• pp.1320-1329
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• 2016

The present study examined the cytotoxic effects of 1, 2, 3, 4, 6-penta-O-galloyl-${\beta}$-D-glucose (PGG), known as the pentahydroxy gallic acid ester of glucose, in the various human cancer cell lines (A-549, MDA-MB-231, U87-MG, MCF-7 and PANC-1), normal MRC-5 fetal fibroblasts, and dental papilla tissue- derived mesenchymal stem cells (DPSCs). Significantly (p<0.05) lower half maximal inhibitory concentration ($IC_{50}$) values were observed in the A-549 and MDA-MB-231 cells showing a high proliferation capacity, compared with other cancer and normal cell lines with a relatively low proliferation capacity. The population doubling time (PDT) was significantly (p<0.05) higher in the $10{\mu}M$ PGG-treated cell lines than those of untreated control cell lines. The present study demonstrated that the $IC_{50}$ value increases proportionally to the extending PDT. A high cell number with senescence-associated ${\beta}-galactosidase$ activity was also observed in the $10{\mu}M$ PGG-treated cells compared with those of untreated control cells. Moreover, the level of telomerase activity was significantly (p<0.05) decreased with $10{\mu}M$ PGG treatment, especially in A-549 and MDA-MB-231 cells showing a high proliferation capacity. Based on these observations, PGG could serve as a potent agent for cancer chemotherapy, as its treatment was more effective in cells with a high proliferation capacity.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.128-134
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• 2004

This experiment was conducted to investigate the effects of dietary pigment sources on the performance, color and antioxidant properties in broiler chick. Experimental diet was formulated to have isocalories and isonitrogen during the experiment period. Total xanthophylls content in the experimental diet was formulated to have 30ppm. Experimental trials were done for five weeks with six treatment groups; T1 (Control), T2 (Olo Glo, natural yellow pigment), T3 (Kern Glo, natural red pigment), T4 (canthaxanthin, synthetic red pigment), T5 (asthaxanthine, natural red pigment), and T6 (seaweed by-products). Body weight gain and feed intake were significantly lower (p＜0.05) in T6 group than in other treatments. Mortality was lower in T2, T3 and T4 than in control, but higher (p＜0.05) in T5 and T6. The sources of pigments did not have any effects on the dressed carcass and abdominal fat pad (p＞0.05). The gizzard weight was significantly lower in T6 (p＜0.05) than in others. Pigmentation of leg skin was significantly lower (p＜0.05) in control and T6. Effects of dietary pigments was greater with red pigments than with yellow pigments, and those were also greater with natural pigments than with synthetic ones. The peroxide value (POV), thiobarbituric acid reactive substances (TBARS) and pH values of chicken meat were increased (p＜0.05) in all treatments at 12 day storage, and was higher (p＜0.05) in pigments supplementation group. No differences of CIE L$\^$*/(lightness) and b$\^$*/(yellowness) were not found by storage days and xanthophylls sources. The a$\^$*/(redness) after 12 day storage was significantly (p＜0.05) decreased in all treatments, but those of T4 and T5 were higher than those of others. These results showed that feeding of xanthophylls sources to chick could improve color intensity and inhibit lipid oxidation of leg meat.