• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7785-7792
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• 2014

Background: Valproic acid (VPA) is a potent anticancer and antiangiogenic agent. However, design and synthesis of chemical derivatives with improved antiangiogenic and anticancer activities are still necessary. In this study a library of novel derivatives of VPA was synthesized and tested. Methods: A human liver cancer cell line (HepG2) and a human normal embryonic kidney cell line (HEK 293) were exposed to various concentrations of VPA derivatives for 24 hours and cell viability was checked by MTT colorimetric assay. Anti-angiogenic properties were evaluated in transgenic zebrafish embryos. Results: N-valproylglycine derivatives suppressed survival almost 70% (p value 0.001) in HepG2 cells but only 10-12% in HEK 293 cells (p value 0.133). They also suppressed angiogenic blood vessel formation by 80% when used between $2-20{\mu}M$ in zebrafish embryos. Valproic acid hydrazides showed moderate level of anticancer activity by affecting 30-50% (p value 0.001) of cell viability in HepG2 cells and 8-10% in HEK293 cells (p value 0.034). Conclusion: The majority of compounds in this study showed potent and stronger antiangiogenic and anticancer activity than VPA. They proved selectively toxic to cancer cells and safer for normal cells. Moreover, these compounds inhibited developmental angiogenesis in zebrafish embryos. Based on the fact that liver is a highly vascularized organ, in case of liver carcinoma these compounds have the potential to target the pathological angiogenesis and could be an effective strategy to treat hepatocellular carcinoma.

• 사상체질의학회지
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• 제18권3호
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• pp.155-165
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• 2006

1. Objectives The purpose of this research is to find out the Constitutional difference of Oxidative stress by Free oxygen radicals. 2. Methods We enrolled 55 patients who visited our hospital for medical examination from February 1, 2006 to May 31, 2006. This research investigated the Constitutional difference of Oxidative stress by Free oxygen radicals. 3. Results and Conclusions l) The Sasang Constitutional distribution of patients have oxidatative stress by Free oxygen radicals was Taeunmin 30%(8persons of total 27persons), Soeumin 56%(9persons of total 16persons), Soyangin 33%(4persons of total 12persons). The rate of patients have oxidatate stress was high in Soeumin. The Sasang Constitution had significance relation with oxidatative stress by Free oxygen radicals. 2) Triglyceride on the average of Taeumin patients have oxidatative stress by Free oxygen radicals is ststistical significantly higher than Triglyceride on the average of controlled normal persons in Taeumin(p-value=0.010) and Soyangin(p-value=0.015). Blood uric acid level on the average of patients have oxidatate stress by Free oxygen radicals is ststistical significantly lower than Blood uric acid level on the average of controlled normal persons in Taeumin(p-value=0.004) and Soyangin(p-value=0.037). This research has shown that there is a statistical significance between the Triglyceride level, Blood uric acid level and oxidatate stress by Free oxygen radicals.

• 한국식품영양과학회지
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• 제31권2호
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• pp.355-360
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• 2002

녹차 추출물의 불용색소를 감마선으로 제거한 후 저장기간 동안의 색상의 환원을 최소화하기 위하여 BHA와 아스콜빈산을 이용하여 실험을 실시하였다. 명도(Hunter color L-value)의 경우 대조구에서는 비조사구보다 조사구가 유의적으로 높아 밝은 색을 보였으며, BHA 첨가구(200 ppm)는 대조구와 유의적으로 차이가 없었다. 아스콜빈산 첨가구(200 ppm)는 대조구와 BHA 첨가구보다 명도가 저장기간 동안 유의적으로 높아 가장 밝은 색을 나타내었다. 저장기간에 따라 대조구, BHA처리구, 아스콜빈산 처리구 등 모두 20 kGy가 10 kGy보다 유의적으로 더 낮은 명도 값을 보여 감마선 조사 선량이 20 kGy또는 그 이상의 고선량일 경우 색의 환원이 증가됨을 알 수 있었다. 적색도(a값)는 감마선 조사선량에 의해 유의적으로 감소하였으며 처리구 중 아스콜빈산 처리구가 가장 낮은 적색도를 나타내어 명도에 이어 적색도도 아스콜빈산 처리가 가장 효과적이었다. 황색도(b값) 또한 유사한 결과를 보여 결론적으로 감마선 조사를 이용하여 녹차 추출물의 색도를 개선시킨 후 아스콜빈산과 같은 항산화제를 이용할 경우 저장 또는 운반 중 색의 환원을 최소화시킬 수 있을 것으로 기대된다.

• 한국수산과학회지
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• 제55권3호
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• pp.239-248
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• 2022

This study was conducted to compare sensory and nutritional and digestive properties of the Israeli carp Cyprinus carpio muscle (ICM) by size [normal large (NLF) and small (NSF) fish], part [ventral (VM) and dorsal (DM) muscle], and commercial value [NLF, recessive (RF) or deformed (DF) fish]. In NLF, the major amino acids were lysine, aspartic acid, and glutamic acid, whereas aspartic acid and glutamic acid were predominant in NSF, DM, VM, DF, and RF. Taurine content increased with fish size, and was higher in DM than in VM, and higher in NLF than in DF but lower than that in RF. Regardless of ICM size, part, and commercial value, potassium and iron were expected in the health function, but calcium and zinc were not expected. The major fatty acids present in ICM were 16:0, 18:1n-9, and 18:2n-6, regardless of size, part, and commercial value. The digestion rate of ICM by digestive organ was lower in NLF than in NSF, and lower in VM with a high lipid content than in DM, and lower in DF than in RF.

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.1-14
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• 2023

Polyethylene terephthalate (PET) is a plastic material commonly applied to beverage packaging used in everyday life. Owing to PET's versatility and ease of use, its consumption has continuously increased, resulting in considerable waste generation. Several physical and chemical recycling processes have been developed to address this problem. Recently, biological upcycling is being actively studied and has come to be regarded as a powerful technology for overcoming the economic issues associated with conventional recycling methods. For upcycling, PET should be degraded into small molecules, such as terephthalic acid and ethylene glycol, which are utilized as substrates for bioconversion, through various degradation processes, including gasification, pyrolysis, and chemical/biological depolymerization. Furthermore, biological upcycling methods have been applied to biosynthesize value-added chemicals, such as adipic acid, muconic acid, catechol, vanillin, and glycolic acid. In this review, we introduce and discuss various degradation methods that yield substrates for bioconversion and biological upcycling processes to produce value-added biochemicals. These technologies encourage a circular economy, which reduces the amount of waste released into the environment.

• 신재생에너지
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• 제19권4호
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• pp.2-10
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• 2023

The current mandatory domestic biodiesel blending ratio is 3.5%, which is planned to be gradually increased to achieve carbon neutrality by 2050. The aim of this study was to improve domestic self-sufficiency in biodiesel raw oil by conducting a technical review on the possibility of utilizing waste oils, such as soup oil, chicken oil, and leather oil, as biodiesel feedstocks. These waste oils have an acid value that is too high to be converted directly into biodiesel. Therefore, a pretreatment to reduce the acid value is necessary. The neutralization process was examined as a potential technology for reducing the acid value. The oil recovery rate of the soup oil after neutralization was significantly low at 37.6 wt%. The oil recovery rates of leather oil and chicken oil were 66.49 wt% and 79.08 wt%, respectively. Based on biodiesel conversion experiment using waste oil with a reduced acid value, the conversions were analyzed as 89 wt%, 91.1 wt%, and 90.5 wt% for soup oil, leather oil, and chicken oil, respectively. Thus, it is technically possible to use soup oil, leather oil, and chicken oil as raw materials for producing biodiesel.

• 생약학회지
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• 제30권3호
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• pp.345-349
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• 1999

Five compounds were isolated from the BuOH fraction of Polygonum bistorta (Polygonaceae). On the basis of spectral data, these compounds were established as caffeic acid, quercimeritrin, avicularin, gallic acid and protocatechuic acid. The inhibitory activies on $3{\alpha}-hydroxysteroid$ dehydrogenase $(3\;{\alpha}-HSD)$ of isolated compounds were compared. $IC_{50}$ value of isolated compounds were $133.57\;{\mu}g/ml\;(caffeic\;acid)$, $89.1\;{\mu}g/ml\;(quercimeritrin)$, $189.85\;{\mu}g/ml\;(avicularin)$, $140.69\;{\mu}g/ml\;(gallic\;acid)$ and $165.27\;{\mu}g/ml\;(protocatechuic\;acid)$ respectively. Although all compounds showed lower inhibition activities than BuOH fraction $(IC_{50}<50\;{\mu}g/ml)$ of Polygonum bistorta, it showed higher inhibition activities than aspirin $(IC_{50}\;246.81\;{\mu}g/ml)$.

• 한국식품위생안전성학회지
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• 제22권4호
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• pp.268-272
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• 2007

본 연구는 식용 번데기를 냉동$(-18^{\circ}C)$, 상온$(25^{\circ}C)$ 및 실온$(35^{\circ}C)$에 저장하면서, 식용 번데기의 기준규격인 산가와 과산화물가의 경시변화를 살펴보고, biogenic amine의 함량과의 상관관계를 살펴봄으로써 식용 번데기의 새로운 품질지표로 biogenic amine을 검토하였다. 번데기 원료에서는 7개의 biogenic amine이 검출되었으며, 그 중 putrescine이 $243.0{\pm}5.4mg/kg$으로 가장 높게 나타났다. 저장기간에 따른 산가 및 과산화물가의 변화에 있어서는 냉동$(-18^{\circ}C)$에서는 저장 7일 동안 변화를 보이지 않았고, 상온$(25^{\circ}C)$에서는 2일, 실온$(35^{\circ}C)$에서는 2일 이전에 번데기 산가의 기준규격치인 5.0을 초과하였다. 또한, 상온$(25^{\circ}C)$에서 저장하는 동안 biogenic amine는 histamine과 tyramine이 유의적으로 증가하는 것으로 나타났고, 이를 바탕으로 산가와 histamine, tyramine의 상관관계를 살펴본 결과 저장기간과 산가는 simple linear식으로, 산가와 histamine, tyramine의 변화는 2차 simple linear관계식으로 제시되었다. 결과적으로 식용 번데기에 있어 산가 이외에 biogenic amine인 histamine과 tyramine이 유의적인 상관관계를 가짐으로써 새로운 품질지표로 사용이 가능하였다.

• 한국조리학회지
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• 제17권4호
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• pp.316-325
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• 2011

튀김 기름은 튀김음식의 품질에 중요한 인자중의 하나이다. 본연구의 목적은 전통적 튀김기와 개량식 튀김기를 사용하여 4일간 연속적으로 사용한 튀김 기름의 품질을 분석하는데 그 목적을 지닌다. 돈가스를 튀긴 후, 뒤김 기름은 일부를 유리병에 분석을 위해 수집하여 여러 이화학적 특성의 실험에 사용하였다. 전통적 튀김기를 사용한 튀김 기름은 사용횟수에 따라 색과 접도 값이 개량식으로 튀긴 기름에 비하여 유의적으로 증가하였다. 유리지방산의 정도와 관련된 산가의 경우, 전통식 튀김기를 사용한 튀김 기름은 개량식 튀김기를 사용한 튀김 기름에 비하여 유의적으로 증가하였다. 반면 이중결합의 농도와 연관이 있는 요오드가는 전통식 튀김기를 사용한 튀김 기름의 값이 개량식을 사용한 튀김 기름에 비하여 유의적으로 감소하였다. 1차지방산화의 지표인 과산화물가의 경우, 전통식 튀김기와 개량식 튀김기를 사용한 튀김기름 모두에서 2일까지는 유의적으로 그 값이 증가하였으나 3 일 부터는 그 값이 감소하였다. 이는 형성된 과산화물이 불안정한 형태이므로 분해를 통하여 다른 2차 산화로 진행된 것으로 사료된다. 2차 산화의 지표인 p-Anisidine 값을 확인한 결과, 전통식 튀김기를 사용한 튀김 기름은 개량식을 사용한 튀김 기름에 비하여 시간 의존적으로 유의적 증가를 나타내었다. 따라서 개량식 튀김기를 사용한 튀김 기름이 유리지방산과 산패의 정도가 전통식 튀김기를 사용한 튀김 기름에 비하여 늦게 진행된는 것으로 사료된다.

• 한국약용작물학회지
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• 제26권6호
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• pp.455-463
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• 2018

Background: Sancho (Zanthoxylum schinifolium Siebold and Zucc) oil is used as a traditional medicinal material to treat severs stomach inflammation and as a diuretic. This study was carried out to investigate the effect of addition of antioxidants and blended oil the storage stability and safety of the biomaterial. Methods and Results: The effects of temperature and light on sancho oil were investigated, and the ability of antioxidants in preventing rancidity of the oil was discovered. Under fluorescent light and in darkness, the acidity of the oil was much lower than that under direct sunlight. The addition of antioxidants decreased the acid value of sancho oil; the antioxidant that showed the best results in this regard was 0.5% propolis. The acid value of canola oil, which had the lowest acid value compared with that of other oils, and blended oil, containing 5% canola oil in sancho oil, decreased by 5.5% and 15%, respectively. About one acid value decrease was observed for every 1% increase in blending with canola oil. As the concentration of canola oil increased, the viscosity and the elightness (L valu) of sancho oil increased slightly, while the blueness (b value) decreased. Conclusions: The results of this study may contribute to ensuring food safety during preservation and the industrialization of the presevation of sancho oil.